ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart52e04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1PPA1_LYCES (P27061) Acid phosphatase 1 precursor (EC 3.1.3.2) (A... 80 2e-15
2VSP2_ARATH (O82122) Vegetative storage protein 2 precursor 74 1e-13
3VSP1_ARATH (O49195) Vegetative storage protein 1 precursor 72 9e-13
4VSPB_SOYBN (P10743) Stem 31 kDa glycoprotein precursor (Vegetati... 68 1e-11
5S25K_SOYBN (P10742) Stem 31 kDa glycoprotein precursor (Vegetati... 66 4e-11
6VSPA_SOYBN (P15490) Stem 28 kDa glycoprotein precursor (Vegetati... 66 4e-11
7CYF_PSINU (Q8WI07) Apocytochrome f precursor 34 0.20
8MMPL8_MYCTU (O07800) Putative membrane protein mmpL8 31 1.7
9RPOB_GRATL (Q6B8R8) DNA-directed RNA polymerase beta chain (EC 2... 30 2.9
10RPOB_CYACA (Q9TM35) DNA-directed RNA polymerase beta chain (EC 2... 29 5.0
11PLB3_CANAL (Q9UVX1) Lysophospholipase 3 precursor (EC 3.1.1.5) (... 29 6.6
12THII_PSEPK (Q88CY4) Thiamine biosynthesis protein thiI 28 8.6

>PPA1_LYCES (P27061) Acid phosphatase 1 precursor (EC 3.1.3.2) (Apase-1(1))|
          Length = 255

 Score = 80.5 bits (197), Expect = 2e-15
 Identities = 34/77 (44%), Positives = 49/77 (63%)
 Frame = +1

Query: 226 CDSWVLGVEAHNVRDWKTVPASCEGYVGHYMLGSHFRRDSKVVIDQALAYVDSLKLAGNG 405
           C +W   VE +N+  WKT+P  C  YV  YM+G  ++ +   V D+A  Y  S+ L  +G
Sbjct: 43  CTTWRFVVETNNLSPWKTIPEECADYVKEYMVGPGYKMEIDRVSDEAGEYAKSVDLGDDG 102

Query: 406 KEVWVFDIDETTLSNLP 456
           ++VW+FD+DET LSNLP
Sbjct: 103 RDVWIFDVDETLLSNLP 119



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>VSP2_ARATH (O82122) Vegetative storage protein 2 precursor|
          Length = 265

 Score = 74.3 bits (181), Expect = 1e-13
 Identities = 35/78 (44%), Positives = 50/78 (64%), Gaps = 1/78 (1%)
 Frame = +1

Query: 226 CDSWVLGVEAHNVRDWKTVPASCEGYVGHYMLGS-HFRRDSKVVIDQALAYVDSLKLAGN 402
           C SW LGVE  N+ D+ TVPA+C+ YV  Y++ S  ++ DSK V  +A  Y   L L  +
Sbjct: 52  CRSWHLGVETSNIIDFDTVPANCKDYVEDYLITSKQYQYDSKTVCKEAYFYAKGLALKND 111

Query: 403 GKEVWVFDIDETTLSNLP 456
              VW+FD+D+T LS++P
Sbjct: 112 TVNVWIFDLDDTLLSSIP 129



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>VSP1_ARATH (O49195) Vegetative storage protein 1 precursor|
          Length = 270

 Score = 71.6 bits (174), Expect = 9e-13
 Identities = 34/78 (43%), Positives = 50/78 (64%), Gaps = 1/78 (1%)
 Frame = +1

Query: 226 CDSWVLGVEAHNVRDWKTVPASCEGYVGHYMLGS-HFRRDSKVVIDQALAYVDSLKLAGN 402
           C SW LGVE  N+ ++ TVPA+C+ YV  Y++ S  ++ DSK V  +A  Y   L L  +
Sbjct: 57  CRSWHLGVETSNIINFDTVPANCKAYVEDYLITSKQYQYDSKTVNKEAYFYAKGLALKND 116

Query: 403 GKEVWVFDIDETTLSNLP 456
              VW+FD+D+T LS++P
Sbjct: 117 TINVWIFDLDDTLLSSIP 134



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>VSPB_SOYBN (P10743) Stem 31 kDa glycoprotein precursor (Vegetative storage|
           protein B)
          Length = 254

 Score = 67.8 bits (164), Expect = 1e-11
 Identities = 38/92 (41%), Positives = 52/92 (56%), Gaps = 1/92 (1%)
 Frame = +1

Query: 184 LRPLLGSGKHAG-VACDSWVLGVEAHNVRDWKTVPASCEGYVGHYMLGSHFRRDSKVVID 360
           LR   G G+ +  V C S+ L VEAHN+R +KT+P  C      Y+ G  FR DSK V  
Sbjct: 28  LRMKTGYGERSSEVKCASFRLAVEAHNIRAFKTIPEECVEPTKDYINGEQFRSDSKTVNQ 87

Query: 361 QALAYVDSLKLAGNGKEVWVFDIDETTLSNLP 456
           QA  Y    ++  N  ++++F ID T LSN+P
Sbjct: 88  QAFFYASEREVHHN--DIFIFGIDNTVLSNIP 117



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>S25K_SOYBN (P10742) Stem 31 kDa glycoprotein precursor (Vegetative storage|
           protein VSP25) (Fragment)
          Length = 291

 Score = 66.2 bits (160), Expect = 4e-11
 Identities = 34/79 (43%), Positives = 46/79 (58%)
 Frame = +1

Query: 220 VACDSWVLGVEAHNVRDWKTVPASCEGYVGHYMLGSHFRRDSKVVIDQALAYVDSLKLAG 399
           V C SW L VEAHN+  ++T+P  C      Y+ G  +R DSK V  QA  Y   L++  
Sbjct: 37  VKCASWRLAVEAHNIFGFETIPEECVEATKEYIHGEQYRSDSKTVNQQAYFYARDLEV-- 94

Query: 400 NGKEVWVFDIDETTLSNLP 456
           + K+ +VF ID T LSN+P
Sbjct: 95  HPKDTFVFSIDGTVLSNIP 113



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>VSPA_SOYBN (P15490) Stem 28 kDa glycoprotein precursor (Vegetative storage|
           protein A)
          Length = 254

 Score = 66.2 bits (160), Expect = 4e-11
 Identities = 34/79 (43%), Positives = 46/79 (58%)
 Frame = +1

Query: 220 VACDSWVLGVEAHNVRDWKTVPASCEGYVGHYMLGSHFRRDSKVVIDQALAYVDSLKLAG 399
           V C SW L VEAHN+  ++T+P  C      Y+ G  +R DSK V  QA  Y   L++  
Sbjct: 40  VKCASWRLAVEAHNIFGFETIPEECVEATKEYIHGEQYRSDSKTVNQQAYFYARDLEV-- 97

Query: 400 NGKEVWVFDIDETTLSNLP 456
           + K+ +VF ID T LSN+P
Sbjct: 98  HPKDTFVFSIDGTVLSNIP 116



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>CYF_PSINU (Q8WI07) Apocytochrome f precursor|
          Length = 321

 Score = 33.9 bits (76), Expect = 0.20
 Identities = 23/84 (27%), Positives = 42/84 (50%), Gaps = 3/84 (3%)
 Frame = +1

Query: 115 VNIRMPTSAAD--VDEAVVA-PLIHALRPLLGSGKHAGVACDSWVLGVEAHNVRDWKTVP 285
           V+I +P S     V EAVV  P    ++ +LG+GK  G+   + ++  E   +  +  +P
Sbjct: 66  VDIEVPQSVFPNTVFEAVVKIPYDKQIKQVLGNGKKGGINVGAVLILPEGFELAPYNRIP 125

Query: 286 ASCEGYVGHYMLGSHFRRDSKVVI 357
           A  +  +G   L  ++R D + +I
Sbjct: 126 AEMKDKIGDLALFQNYRPDKRNII 149



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>MMPL8_MYCTU (O07800) Putative membrane protein mmpL8|
          Length = 1089

 Score = 30.8 bits (68), Expect = 1.7
 Identities = 18/50 (36%), Positives = 26/50 (52%)
 Frame = +1

Query: 244 GVEAHNVRDWKTVPASCEGYVGHYMLGSHFRRDSKVVIDQALAYVDSLKL 393
           G+  +N  D KT+PAS E  +G+  L  HF   S ++I + L    S  L
Sbjct: 418 GLARYNYDDRKTLPASVESSIGYAALDKHF--PSNLIIPEYLFIQSSTDL 465



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>RPOB_GRATL (Q6B8R8) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase beta subunit) (RNA
           polymerase beta subunit)
          Length = 1120

 Score = 30.0 bits (66), Expect = 2.9
 Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 5/70 (7%)
 Frame = +1

Query: 169 PLIHALRPLLGSGKHAGVACDSWVLGVEAHN-----VRDWKTVPASCEGYVGHYMLGSHF 333
           PL+   + ++G+G  A +A DS +  +   N     V   K    + EGY  HY L  ++
Sbjct: 591 PLLFPEKSIVGTGLEAKIAKDSGMTIISRTNGTVSYVSGTKIGIQNKEGYTIHYRLKKYY 650

Query: 334 RRDSKVVIDQ 363
           R +    I+Q
Sbjct: 651 RSNQDTCINQ 660



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>RPOB_CYACA (Q9TM35) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase beta subunit) (RNA
           polymerase beta subunit)
          Length = 1081

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 13/31 (41%), Positives = 19/31 (61%)
 Frame = +1

Query: 169 PLIHALRPLLGSGKHAGVACDSWVLGVEAHN 261
           PLI   RPL+G+G  A +A DS ++ +   N
Sbjct: 552 PLIFPERPLVGTGLEAQIAKDSGIMAISRSN 582



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>PLB3_CANAL (Q9UVX1) Lysophospholipase 3 precursor (EC 3.1.1.5) (Phospholipase|
           B 3)
          Length = 754

 Score = 28.9 bits (63), Expect = 6.6
 Identities = 24/79 (30%), Positives = 35/79 (44%), Gaps = 1/79 (1%)
 Frame = +1

Query: 178 HALRPLLGSGKHA-GVACDSWVLGVEAHNVRDWKTVPASCEGYVGHYMLGSHFRRDSKVV 354
           H L  LL S  +  G++  +W++G  A N  DW +V     G    + L       S + 
Sbjct: 209 HGLGGLLDSSTYVVGLSGGNWLVGSLALN--DWLSVGDIVNGKSTIWQLQDSILNPSGMR 266

Query: 355 IDQALAYVDSLKLAGNGKE 411
           ID+ +AY   L  A   KE
Sbjct: 267 IDKTIAYYYGLAQAVQAKE 285



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>THII_PSEPK (Q88CY4) Thiamine biosynthesis protein thiI|
          Length = 484

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 11/31 (35%), Positives = 18/31 (58%)
 Frame = +1

Query: 241 LGVEAHNVRDWKTVPASCEGYVGHYMLGSHF 333
           L VE + + D+  + A C+ + GH + G HF
Sbjct: 79  LQVEEYPLGDFDDIVAKCKHHFGHLLAGKHF 109


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.317    0.130    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,164,644
Number of Sequences: 219361
Number of extensions: 811427
Number of successful extensions: 3029
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2921
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3017
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2909956200
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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