Clone Name | bart50a04 |
---|---|
Clone Library Name | barley_pub |
>ZN363_MOUSE (Q9CR50) RING finger and CHY zinc finger domain-containing protein| 1 (Zinc finger protein 363) (CH-rich-interacting match with PLAG1) (Androgen receptor N-terminal-interacting protein) Length = 261 Score = 81.6 bits (200), Expect = 8e-16 Identities = 34/95 (35%), Positives = 49/95 (51%) Frame = +3 Query: 168 GQMDLGAEQHGCEHYTRRCRIRAPCCGEVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISK 347 G +L GCEHY R C ++APCC +++ CR CH D + H + R ++ + Sbjct: 9 GVRNLAQGPRGCEHYDRACLLKAPCCDKLYTCRLCH---------DTNEDHQLDRFKVKE 59 Query: 348 VICSLCNKEQDVQKNCSGCGACMGKYFCEKCNFFD 452 V C C K Q Q+ C C G+Y+C C+ FD Sbjct: 60 VQCINCEKLQHAQQTCEDCSTLFGEYYCSICHLFD 94
>ZN363_HUMAN (Q96PM5) RING finger and CHY zinc finger domain-containing protein| 1 (Zinc finger protein 363) (CH-rich-interacting match with PLAG1) (Androgen receptor N-terminal-interacting protein) Length = 261 Score = 81.3 bits (199), Expect = 1e-15 Identities = 32/87 (36%), Positives = 49/87 (56%) Frame = +3 Query: 192 QHGCEHYTRRCRIRAPCCGEVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISKVICSLCNK 371 Q GCEHY R C ++APCC +++ CR CH+ ++ H + R ++ +V C C K Sbjct: 17 QRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNED---------HQLDRFKVKEVQCINCEK 67 Query: 372 EQDVQKNCSGCGACMGKYFCEKCNFFD 452 Q Q+ C C G+Y+C+ C+ FD Sbjct: 68 IQHAQQTCEECSTLFGEYYCDICHLFD 94
>TRPA_MAIZE (P42390) Indole-3-glycerol phosphate lyase, chloroplast precursor| (EC 4.1.2.-) (Indole synthase) (Tryptophan synthase alpha chain) (EC 4.2.1.20) (Benzoxazineless 1) Length = 347 Score = 35.4 bits (80), Expect = 0.069 Identities = 23/55 (41%), Positives = 33/55 (60%), Gaps = 3/55 (5%) Frame = -3 Query: 231 VSCTSSCNARSRAA---LPPNPSVRPSPAFVPPRGDAAPPEERKEAGVRESLAAL 76 V T++ AR+ AA +P P P+PA VPP+ AA P ER+ V +++AAL Sbjct: 44 VVVTTTTTARAAAAAVTVPAAPPQAPAPAPVPPKQAAA-PAERRSRPVSDTMAAL 97
>HOT13_KLULA (Q6CIK2) Helper of Tim protein 13| Length = 114 Score = 33.9 bits (76), Expect = 0.20 Identities = 18/61 (29%), Positives = 27/61 (44%), Gaps = 3/61 (4%) Frame = +3 Query: 201 CEHYTRRCRI---RAPCCGEVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISKVICSLCNK 371 CEH+ + R CC + C CH + L V +R++I + VIC +C Sbjct: 17 CEHWHGPLDVIALRFKCCNGYYACYECHQE----LVPHVTERYNINDETVPLVICGVCKL 72 Query: 372 E 374 E Sbjct: 73 E 73
>RSPO1_BRARE (Q6DHR0) R-spondin-1 precursor (Roof plate-specific spondin-1)| Length = 261 Score = 33.1 bits (74), Expect = 0.34 Identities = 24/89 (26%), Positives = 35/89 (39%), Gaps = 7/89 (7%) Frame = +3 Query: 195 HGCEHYTRRCRIRAPCCGEVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISKVIC-----S 359 +GCEH C E GC C + +L+R+DI + I C Sbjct: 43 NGCEH-----------CSEYNGCLKCRPRL-----FILLERNDIRQIGICLAACPVGYYG 86 Query: 360 LCNKEQDVQKNCS--GCGACMGKYFCEKC 440 + N++ + C C AC + FC KC Sbjct: 87 IRNRDMNKCTQCKIENCEACFSRNFCTKC 115
>HCN2_HUMAN (Q9UL51) Potassium/sodium hyperpolarization-activated cyclic| nucleotide-gated channel 2 (Brain cyclic nucleotide gated channel 2) (BCNG-2) Length = 889 Score = 32.3 bits (72), Expect = 0.58 Identities = 17/36 (47%), Positives = 19/36 (52%) Frame = -3 Query: 192 ALPPNPSVRPSPAFVPPRGDAAPPEERKEAGVRESL 85 A PP P P+P PPR +A PPE E G R L Sbjct: 42 APPPGPG--PAPPQHPPRAEALPPEAADEGGPRGRL 75
>CENPB_HUMAN (P07199) Major centromere autoantigen B (Centromere protein B)| (CENP-B) Length = 599 Score = 32.0 bits (71), Expect = 0.76 Identities = 15/32 (46%), Positives = 18/32 (56%) Frame = -3 Query: 231 VSCTSSCNARSRAALPPNPSVRPSPAFVPPRG 136 VSC+ AR+R A P P+ SPA VP G Sbjct: 133 VSCSGVARARARNAAPRTPAAPASPAAVPSEG 164
>NIFA_AZOBR (P30667) Nif-specific regulatory protein| Length = 625 Score = 32.0 bits (71), Expect = 0.76 Identities = 17/44 (38%), Positives = 23/44 (52%) Frame = -3 Query: 216 SCNARSRAALPPNPSVRPSPAFVPPRGDAAPPEERKEAGVRESL 85 +C SRA LPP + PSPA PP P +E + +R+ L Sbjct: 544 ACPCPSRAPLPPQ-APPPSPAAAPPPAAEVPLDEPESGSLRDRL 586
>ARI3A_HUMAN (Q99856) AT-rich interactive domain-containing protein 3A (ARID| domain-containing protein 3A) (B-cell regulator of IgH transcription) (Bright) (E2F-binding protein 1) Length = 593 Score = 31.2 bits (69), Expect = 1.3 Identities = 18/55 (32%), Positives = 24/55 (43%) Frame = -3 Query: 201 SRAALPPNPSVRPSPAFVPPRGDAAPPEERKEAGVRESLAALXXXXXXXXXPAGI 37 +R LPP+P PA P R AAP E+R+ R A + AG+ Sbjct: 23 ARQQLPPDP-----PAAPPGRARAAPDEDREPESARMQRAQMAALAAMRAAAAGL 72
>TGT_PASMU (P57831) Queuine tRNA-ribosyltransferase (EC 2.4.2.29)| (tRNA-guanine transglycosylase) (Guanine insertion enzyme) Length = 385 Score = 31.2 bits (69), Expect = 1.3 Identities = 13/40 (32%), Positives = 20/40 (50%) Frame = +3 Query: 147 ARKRETAGQMDLGAEQHGCEHYTRRCRIRAPCCGEVFGCR 266 A+ RE +D + + C+HYT+ CGE+ G R Sbjct: 293 AKYREDTSPLDPECDCYTCKHYTKAYLYHLDKCGEILGAR 332
>NHS_HUMAN (Q6T4R5) Nance-Horan syndrome protein (Congenital cataracts and| dental anomalies protein) Length = 1630 Score = 30.4 bits (67), Expect = 2.2 Identities = 17/53 (32%), Positives = 23/53 (43%), Gaps = 2/53 (3%) Frame = -3 Query: 195 AALPPNPSVRPSPA--FVPPRGDAAPPEERKEAGVRESLAALXXXXXXXXXPA 43 + LPP P P+PA PP G+A+ E AG+ E+ A A Sbjct: 65 SGLPPPPPPLPAPADQTQPPHGEASVAGEESTAGIPEAAPAAGEASSAAAAAA 117
>HOT13_YEAST (P36078) Helper of Tim protein 13| Length = 116 Score = 30.4 bits (67), Expect = 2.2 Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 2/51 (3%) Frame = +3 Query: 228 IRAPCCGEVFGCRHCHNQ-AKNSLE-VDVLDRHDIPRHEISKVICSLCNKE 374 IR CC + + C CH + + + LE D+LD D +H +IC +C E Sbjct: 29 IRFKCCDKYYACFECHQELSSHPLEKYDLLD--DANKH---LIICGVCRHE 74
>SOX9_PONPY (P61754) Transcription factor SOX-9| Length = 509 Score = 30.0 bits (66), Expect = 2.9 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -3 Query: 186 PPNPSVRPSPAFVPPRGDAAPPEE 115 PP P P P PP+ AAPP++ Sbjct: 353 PPAPQAPPQPQAAPPQQPAAPPQQ 376
>SOX9_PANTR (Q9BG89) Transcription factor SOX-9| Length = 509 Score = 30.0 bits (66), Expect = 2.9 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -3 Query: 186 PPNPSVRPSPAFVPPRGDAAPPEE 115 PP P P P PP+ AAPP++ Sbjct: 353 PPAPQAPPQPQAAPPQQPAAPPQQ 376
>SOX9_MACMU (P61753) Transcription factor SOX-9| Length = 509 Score = 30.0 bits (66), Expect = 2.9 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -3 Query: 186 PPNPSVRPSPAFVPPRGDAAPPEE 115 PP P P P PP+ AAPP++ Sbjct: 353 PPAPQAPPQPQAAPPQQPAAPPQQ 376
>SOX9_HUMAN (P48436) Transcription factor SOX-9| Length = 509 Score = 30.0 bits (66), Expect = 2.9 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -3 Query: 186 PPNPSVRPSPAFVPPRGDAAPPEE 115 PP P P P PP+ AAPP++ Sbjct: 353 PPAPQAPPQPQAAPPQQPAAPPQQ 376
>SOX9_CALJA (Q9BG91) Transcription factor SOX-9| Length = 509 Score = 30.0 bits (66), Expect = 2.9 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -3 Query: 186 PPNPSVRPSPAFVPPRGDAAPPEE 115 PP P P P PP+ AAPP++ Sbjct: 353 PPAPQAPPQPQAAPPQQPAAPPQQ 376
>PCSK5_MOUSE (Q04592) Proprotein convertase subtilisin/kexin type 5 precursor| (EC 3.4.21.-) (Proprotein convertase PC5) (Subtilisin/kexin-like protease PC5) (PC6) (Subtilisin-like proprotein convertase 6) (SPC6) Length = 1877 Score = 30.0 bits (66), Expect = 2.9 Identities = 24/85 (28%), Positives = 34/85 (40%), Gaps = 10/85 (11%) Frame = +3 Query: 216 RRCRIRAPCCGEVFG-----CRHCH-----NQAKNSLEVDVLDRHDIPRHEISKVICSLC 365 +RCR AP C FG C C N+ +S V D +I K +C C Sbjct: 686 KRCRKCAPNCESCFGSHGDQCLSCKYGYFLNEETSSC---VTQCPDGSYEDIKKNVCGKC 742 Query: 366 NKEQDVQKNCSGCGACMGKYFCEKC 440 ++ C AC+G + C +C Sbjct: 743 SE---------NCKACIGFHNCTEC 758
>CLPX_LEPIN (Q8F353) ATP-dependent Clp protease ATP-binding subunit clpX| Length = 420 Score = 30.0 bits (66), Expect = 2.9 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = +3 Query: 345 KVICSLCNKEQDVQKNCSGCGACMGKYFCEKC 440 K+ CS C KEQD K A G Y C++C Sbjct: 13 KLFCSFCGKEQDAVKRLV---AGPGVYICDEC 41
>CLPX_LEPIC (Q72SG5) ATP-dependent Clp protease ATP-binding subunit clpX| Length = 420 Score = 30.0 bits (66), Expect = 2.9 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = +3 Query: 345 KVICSLCNKEQDVQKNCSGCGACMGKYFCEKC 440 K+ CS C KEQD K A G Y C++C Sbjct: 13 KLFCSFCGKEQDAVKRLV---AGPGVYICDEC 41
>TGT_MANSM (Q65S94) Queuine tRNA-ribosyltransferase (EC 2.4.2.29)| (tRNA-guanine transglycosylase) (Guanine insertion enzyme) Length = 379 Score = 29.3 bits (64), Expect = 4.9 Identities = 11/40 (27%), Positives = 20/40 (50%) Frame = +3 Query: 147 ARKRETAGQMDLGAEQHGCEHYTRRCRIRAPCCGEVFGCR 266 A+ ++ +D + + C+HYT+ CGE+ G R Sbjct: 293 AKYKDDTSPLDPHCDCYTCQHYTKSYLYHLDKCGEILGAR 332
>CHEB2_CAUCR (Q9AAK0) Chemotaxis response regulator protein-glutamate| methylesterase of group 2 operon (EC 3.1.1.61) Length = 347 Score = 29.3 bits (64), Expect = 4.9 Identities = 13/29 (44%), Positives = 14/29 (48%) Frame = -3 Query: 201 SRAALPPNPSVRPSPAFVPPRGDAAPPEE 115 +RAA P P P P PP D A P E Sbjct: 129 ARAARPAPPQAAPRPTLAPPSSDPAGPIE 157
>H6ST3_HUMAN (Q8IZP7) Heparan-sulfate 6-O-sulfotransferase 3 (EC 2.8.2.-)| (HS6ST-3) Length = 471 Score = 29.3 bits (64), Expect = 4.9 Identities = 24/68 (35%), Positives = 26/68 (38%), Gaps = 25/68 (36%) Frame = -3 Query: 228 SCTSSCN------------------ARSRAALPPN-----PSVRPSPAFVP--PRGDAAP 124 SCTSSC RA PP P + P P P PRG AAP Sbjct: 29 SCTSSCTNLGEQPRAGEAGPPAVPGPARRAQAPPEEWERRPQLPPPPRGPPEGPRGAAAP 88 Query: 123 PEERKEAG 100 EE +E G Sbjct: 89 EEEDEEPG 96
>CYC3_DESAC (P00137) Cytochrome c3 (Cytochrome c7) (Cytochrome c551.5)| Length = 68 Score = 29.3 bits (64), Expect = 4.9 Identities = 14/52 (26%), Positives = 18/52 (34%) Frame = +3 Query: 249 EVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISKVICSLCNKEQDVQKNCSGC 404 E GC CH + +D H K C C+K + C GC Sbjct: 22 EKLGCDACHEGTPAKIAIDKKSAH--------KDACKTCHKSNNGPTKCGGC 65
>FIP1_HUMAN (Q6UN15) Pre-mRNA 3'-end-processing factor FIP1 (FIP1-like 1)| (Factor interacting with PAP) (hFip1) (Rearranged in hypereosinophilia) Length = 594 Score = 28.9 bits (63), Expect = 6.4 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = -3 Query: 189 LPPNPSVRPSPAFVPPRGDAAPP 121 LPP P+V +P +PP G PP Sbjct: 375 LPPPPTVSTAPPLIPPPGFPPPP 397
>PRDM5_MOUSE (Q9CXE0) PR domain zinc finger protein 5 (PR domain-containing| protein 5) Length = 599 Score = 28.9 bits (63), Expect = 6.4 Identities = 14/41 (34%), Positives = 19/41 (46%) Frame = +3 Query: 249 EVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISKVICSLCNK 371 E+F C+ C K + + L RH I E C +CNK Sbjct: 287 EIFDCQEC---MKKFISANQLKRHMITHSEKRPYNCEICNK 324
>CWC24_CANGA (Q6FXX1) Pre-mRNA-splicing factor CWC24| Length = 226 Score = 28.9 bits (63), Expect = 6.4 Identities = 22/74 (29%), Positives = 34/74 (45%), Gaps = 8/74 (10%) Frame = +3 Query: 243 CGEVFGCRHCHNQ----AKNSLEVD--VLDRHDIPRHEISKVICSLCNK--EQDVQKNCS 398 CG C+ H++ A +L D + ++ + R E C LC + E+ V+ NC Sbjct: 122 CGYGDSCKFLHSRDDFKAGWTLATDWKIDEQKEETRKEAVPFKCVLCKESYERPVKTNC- 180 Query: 399 GCGACMGKYFCEKC 440 G YFC+KC Sbjct: 181 ------GHYFCQKC 188
>FIP1_RAT (Q5U317) Pre-mRNA 3'-end-processing factor FIP1 (FIP1-like 1)| Length = 536 Score = 28.9 bits (63), Expect = 6.4 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = -3 Query: 189 LPPNPSVRPSPAFVPPRGDAAPP 121 LPP P+V +P +PP G PP Sbjct: 315 LPPPPTVSTAPPLIPPPGFPPPP 337
>PRDM5_HUMAN (Q9NQX1) PR domain zinc finger protein 5 (PR domain-containing| protein 5) Length = 630 Score = 28.9 bits (63), Expect = 6.4 Identities = 14/41 (34%), Positives = 19/41 (46%) Frame = +3 Query: 249 EVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISKVICSLCNK 371 E+F C+ C K + + L RH I E C +CNK Sbjct: 318 EIFDCQEC---MKKFISANQLKRHMITHSEKRPYNCEICNK 355
>SYA_PICTO (Q6L1Z4) Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine--tRNA ligase)| (AlaRS) Length = 870 Score = 28.9 bits (63), Expect = 6.4 Identities = 15/39 (38%), Positives = 19/39 (48%) Frame = +3 Query: 276 NQAKNSLEVDVLDRHDIPRHEISKVICSLCNKEQDVQKN 392 N N + D + IP ISKV L NKE D+ +N Sbjct: 425 NTISNENIAKIYDTYGIPLEIISKVFKDLTNKELDIPEN 463
>F112B_MOUSE (Q9DAN6) Protein FAM112B| Length = 167 Score = 28.9 bits (63), Expect = 6.4 Identities = 24/71 (33%), Positives = 28/71 (39%), Gaps = 4/71 (5%) Frame = +3 Query: 207 HYTRRCRIRAPCCGEVFGCRHCHNQAKNSLEVDVLD-RHDIPRHEISKVICSLCNK---E 374 H R CR + CR H N L + RH +PR EIS I S +K E Sbjct: 23 HQIRACRFPY----HLIKCRKNHPDVANKLATCPFNARHQVPRAEISHHISSCDDKSCIE 78 Query: 375 QDVQKNCSGCG 407 QDV G Sbjct: 79 QDVVNQTRNLG 89
>F112B_BOVIN (Q3SZU0) Protein FAM112B| Length = 167 Score = 28.9 bits (63), Expect = 6.4 Identities = 24/71 (33%), Positives = 28/71 (39%), Gaps = 4/71 (5%) Frame = +3 Query: 207 HYTRRCRIRAPCCGEVFGCRHCHNQAKNSLEVDVLD-RHDIPRHEISKVICSLCNK---E 374 H R CR + CR H N L + RH +PR EIS I S +K E Sbjct: 23 HQIRACRFPY----HLIKCRKNHPDVANKLATCPFNARHQVPRAEISHHISSCDDKSCIE 78 Query: 375 QDVQKNCSGCG 407 QDV G Sbjct: 79 QDVVNQTRSLG 89
>RIMS1_RAT (Q9JIR4) Regulating synaptic membrane exocytosis protein 1| (Rab3-interacting molecule 1) (RIM 1) Length = 1615 Score = 28.5 bits (62), Expect = 8.4 Identities = 19/47 (40%), Positives = 22/47 (46%) Frame = -3 Query: 231 VSCTSSCNARSRAALPPNPSVRPSPAFVPPRGDAAPPEERKEAGVRE 91 VS S AR+ AA PP P P P G A PPE R +R+ Sbjct: 442 VSPPESPRARAAAAQPPTEHGPPPPR--PAPGPAEPPEPRVPEPLRK 486
>TRAF2_HUMAN (Q12933) TNF receptor-associated factor 2 (Tumor necrosis factor| type 2 receptor-associated protein 3) Length = 501 Score = 28.5 bits (62), Expect = 8.4 Identities = 23/72 (31%), Positives = 26/72 (36%), Gaps = 1/72 (1%) Frame = +3 Query: 192 QHGCEHYTRRCR-IRAPCCGEVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISKVICSLCN 368 +H C + CR RAPCCG H K L D + IPR Sbjct: 153 EHECPERSLSCRHCRAPCCGADVKAHH-EVCPKFPLTCDGCGKKKIPR-----------E 200 Query: 369 KEQDVQKNCSGC 404 K QD K C C Sbjct: 201 KFQDHVKTCGKC 212
>NKG2D_RAT (O70215) NKG2-D type II integral membrane protein| (NKG2-D-activating NK receptor) (NK cell receptor D) (Killer cell lectin-like receptor subfamily K member 1) (NKR-P2) (NK lectin-like receptor) (NKLLR) (CD314 antigen) Length = 215 Score = 28.5 bits (62), Expect = 8.4 Identities = 12/33 (36%), Positives = 15/33 (45%) Frame = +3 Query: 351 ICSLCNKEQDVQKNCSGCGACMGKYFCEKCNFF 449 I LCNKE V CG C + C + N + Sbjct: 78 ITLLCNKEVSVSSREGYCGPCPNDWICHRNNCY 110
>FUR2_DROME (P30432) Furin-like protease 2 precursor (EC 3.4.21.75) (Furin-2)| Length = 1679 Score = 28.5 bits (62), Expect = 8.4 Identities = 17/72 (23%), Positives = 26/72 (36%), Gaps = 10/72 (13%) Frame = +3 Query: 255 FGCRHCHNQAKNSLEVDVLDRHDIPRHE-ISKVICSLCNKEQDVQKNCSGCGAC------ 413 FGC+ CH+ K + L P H + +C C Q + C C Sbjct: 1349 FGCQKCHHYCKTCNDAGPLACTSCPPHSMLDGGLCMECLSSQYYDTTSATCKTCHDSCRS 1408 Query: 414 ---MGKYFCEKC 440 G++ C+ C Sbjct: 1409 CFGPGQFSCKGC 1420
>RNZ_BACTN (Q89ZN1) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNase Z) (tRNA 3| endonuclease) Length = 316 Score = 28.5 bits (62), Expect = 8.4 Identities = 13/42 (30%), Positives = 24/42 (57%) Frame = +3 Query: 225 RIRAPCCGEVFGCRHCHNQAKNSLEVDVLDRHDIPRHEISKV 350 R R PCCG +F Q N + D++D + +P +E++++ Sbjct: 142 RHRIPCCGFLFE----EKQRPNHIIRDMVDFYKVPVYELNRI 179 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.316 0.121 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 53,352,709 Number of Sequences: 219361 Number of extensions: 900314 Number of successful extensions: 5830 Number of sequences better than 10.0: 37 Number of HSP's better than 10.0 without gapping: 4956 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5759 length of database: 80,573,946 effective HSP length: 102 effective length of database: 58,199,124 effective search space used: 2851757076 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)