ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart49c11
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SMT2_ORYSA (O82427) 24-methylenesterol C-methyltransferase 2 (EC... 158 3e-39
2SMT2_ARATH (Q39227) 24-methylenesterol C-methyltransferase 2 (EC... 127 6e-30
3SMT3B_ARATH (Q94JS4) 24-methylenesterol C-methyltransferase 3 (E... 118 4e-27
4SMT1_ORYSA (Q6ZIX2) Cycloartenol-C-24-methyltransferase 1 (EC 2.... 51 9e-07
5ERG6_MAGGR (Q5EN22) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 50 1e-06
6ERG6_NEUCR (Q9P3R1) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 49 3e-06
7ERG6_YARLI (Q6C2D9) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 49 3e-06
8ERG6_CLALS (Q875K1) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 48 6e-06
9ERG6_SCHPO (O14321) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 48 6e-06
10ERG6_CANAL (O74198) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 47 2e-05
11ERG6_DEBHA (Q6BRB7) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 47 2e-05
12SMT1_ARATH (Q9LM02) Cycloartenol-C-24-methyltransferase (EC 2.1.... 44 9e-05
13ERG6_KLULA (Q6CYB3) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 44 9e-05
14ERG6_YEAST (P25087) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 44 1e-04
15ERG6_ASHGO (Q759S7) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 44 1e-04
16ERG6_CANGA (Q6FRZ7) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 44 1e-04
17ERG6_PNECA (Q96WX4) Sterol 24-C-methyltransferase (EC 2.1.1.41) ... 39 0.004
18RAD5_DEBHA (Q6BIP2) DNA repair protein RAD5 (EC 3.6.1.-) 30 1.7
19AREA_GIBFU (P78688) Nitrogen regulatory protein areA 28 4.8
20AMPM1_SCHPO (O59730) Probable methionine aminopeptidase 1 (EC 3.... 28 8.2

>SMT2_ORYSA (O82427) 24-methylenesterol C-methyltransferase 2 (EC 2.1.1.143)|
           (24-sterol C-methyltransferase 2)
           (Sterol-C-methyltransferase 2)
          Length = 363

 Score =  158 bits (400), Expect = 3e-39
 Identities = 72/80 (90%), Positives = 78/80 (97%)
 Frame = +1

Query: 1   KRAVDLKMGSITRDKVQDKYTQYWSFFRPPQETATTQASADQVPAFVDTFYNLVTDIYEW 180
           KRAVDLKMGSIT DKV+DKYTQYWSFFR P+ETATT+ASA++VPAFVDTFYNLVTDIYEW
Sbjct: 33  KRAVDLKMGSITNDKVKDKYTQYWSFFRRPKETATTEASAEKVPAFVDTFYNLVTDIYEW 92

Query: 181 GWGQSFHFSPSLPGRSHRDA 240
           GWGQSFHFSPSLPGRSHR+A
Sbjct: 93  GWGQSFHFSPSLPGRSHREA 112



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>SMT2_ARATH (Q39227) 24-methylenesterol C-methyltransferase 2 (EC 2.1.1.143)|
           (24-sterol C-methyltransferase 2)
           (Sterol-C-methyltransferase 2)
          Length = 361

 Score =  127 bits (320), Expect = 6e-30
 Identities = 59/80 (73%), Positives = 67/80 (83%)
 Frame = +1

Query: 1   KRAVDLKMGSITRDKVQDKYTQYWSFFRPPQETATTQASADQVPAFVDTFYNLVTDIYEW 180
           KRAVDL  GSI+ +KVQD Y QYWSFFR P+E  T    A++VP FVDTFYNLVTDIYEW
Sbjct: 32  KRAVDLSGGSISAEKVQDNYKQYWSFFRRPKEIET----AEKVPDFVDTFYNLVTDIYEW 87

Query: 181 GWGQSFHFSPSLPGRSHRDA 240
           GWGQSFHFSPS+PG+SH+DA
Sbjct: 88  GWGQSFHFSPSIPGKSHKDA 107



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>SMT3B_ARATH (Q94JS4) 24-methylenesterol C-methyltransferase 3 (EC 2.1.1.143)|
           (24-sterol C-methyltransferase 3)
           (Sterol-C-methyltransferase 3)
          Length = 359

 Score =  118 bits (296), Expect = 4e-27
 Identities = 55/80 (68%), Positives = 64/80 (80%)
 Frame = +1

Query: 1   KRAVDLKMGSITRDKVQDKYTQYWSFFRPPQETATTQASADQVPAFVDTFYNLVTDIYEW 180
           KRA DL  GSI+ +KV+D Y QYWSFFR P+E      SA++VP FVDTFYNLVTDIYEW
Sbjct: 32  KRASDLSGGSISAEKVKDNYNQYWSFFRKPKEIE----SAEKVPDFVDTFYNLVTDIYEW 87

Query: 181 GWGQSFHFSPSLPGRSHRDA 240
           GWGQSFHFSP +PG+S +DA
Sbjct: 88  GWGQSFHFSPHVPGKSDKDA 107



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>SMT1_ORYSA (Q6ZIX2) Cycloartenol-C-24-methyltransferase 1 (EC 2.1.1.41)|
           (24-sterol C-methyltransferase 1) (Sterol
           C-methyltransferase 1)
          Length = 344

 Score = 50.8 bits (120), Expect = 9e-07
 Identities = 26/75 (34%), Positives = 42/75 (56%), Gaps = 3/75 (4%)
 Frame = +1

Query: 25  GSITRDKVQ---DKYTQYWSFFRPPQETATTQASADQVPAFVDTFYNLVTDIYEWGWGQS 195
           G IT+D+V+   D+Y +Y  ++   +E     A        V+ +Y+L T  YE+GWG+S
Sbjct: 15  GKITKDEVKSAVDEYEKYHGYYGGKEE-----ARKSNYTDMVNKYYDLATSFYEYGWGES 69

Query: 196 FHFSPSLPGRSHRDA 240
           FHF+    G S R++
Sbjct: 70  FHFAHRWNGESLRES 84



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>ERG6_MAGGR (Q5EN22) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 390

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 26/65 (40%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
 Frame = +1

Query: 52  DKYTQYWSFFRPPQETATT--QASADQVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGR 225
           D+Y ++W       ET     +  AD        +YNLVTD YE+GWGQSFHF    PG 
Sbjct: 55  DQYFRHWDGKTAKTETTKVREERKADYA-TLTRQYYNLVTDFYEYGWGQSFHFCTFAPGE 113

Query: 226 SHRDA 240
           +   A
Sbjct: 114 TFASA 118



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>ERG6_NEUCR (Q9P3R1) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 379

 Score = 48.9 bits (115), Expect = 3e-06
 Identities = 27/76 (35%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
 Frame = +1

Query: 16  LKMGSITRDKVQDKYTQYWSFFRPPQET-ATTQASADQVPAFVDTFYNLVTDIYEWGWGQ 192
           LK  +  +    D+Y +++       ET A  +A   +       +YNL TDIYE+GWGQ
Sbjct: 37  LKKNTEAQQIAVDEYFKHFDNKTAEAETQADREARTKEYATLTRHYYNLATDIYEYGWGQ 96

Query: 193 SFHFSPSLPGRSHRDA 240
            FHF    PG S   A
Sbjct: 97  CFHFCRYSPGESFYQA 112



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>ERG6_YARLI (Q6C2D9) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 381

 Score = 48.9 bits (115), Expect = 3e-06
 Identities = 22/49 (44%), Positives = 26/49 (53%)
 Frame = +1

Query: 94  ETATTQASADQVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDA 240
           E A  Q   +        +YNLVTD YE+GWG SFHFS    G + R A
Sbjct: 64  EDADEQGRIEDYAGLTKHYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQA 112



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>ERG6_CLALS (Q875K1) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 375

 Score = 48.1 bits (113), Expect = 6e-06
 Identities = 20/40 (50%), Positives = 24/40 (60%)
 Frame = +1

Query: 121 DQVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDA 240
           D   +    +YNLVTD YE+GWG SFHFS    G + R A
Sbjct: 67  DDYSSLTKNYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQA 106



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>ERG6_SCHPO (O14321) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 378

 Score = 48.1 bits (113), Expect = 6e-06
 Identities = 22/66 (33%), Positives = 38/66 (57%), Gaps = 3/66 (4%)
 Frame = +1

Query: 40  DKVQDKYTQYWSFFRPPQETATTQASADQVPAF---VDTFYNLVTDIYEWGWGQSFHFSP 210
           D+   K  +Y+ F+    E  + +  A ++  +   V+++Y+L TD+YE+GW QSFHFS 
Sbjct: 39  DEQSRKLQEYFEFWDRNHENESEEDRARRIDGYKSVVNSYYDLATDLYEYGWSQSFHFSR 98

Query: 211 SLPGRS 228
              G +
Sbjct: 99  FYKGEA 104



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>ERG6_CANAL (O74198) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 376

 Score = 46.6 bits (109), Expect = 2e-05
 Identities = 19/31 (61%), Positives = 22/31 (70%)
 Frame = +1

Query: 148 FYNLVTDIYEWGWGQSFHFSPSLPGRSHRDA 240
           +YNLVTD YE+GWG SFHFS    G + R A
Sbjct: 76  YYNLVTDFYEYGWGSSFHFSRYYKGEAFRQA 106



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>ERG6_DEBHA (Q6BRB7) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 377

 Score = 46.6 bits (109), Expect = 2e-05
 Identities = 19/31 (61%), Positives = 22/31 (70%)
 Frame = +1

Query: 148 FYNLVTDIYEWGWGQSFHFSPSLPGRSHRDA 240
           +YNLVTD YE+GWG SFHFS    G + R A
Sbjct: 77  YYNLVTDFYEYGWGSSFHFSRYYTGEAFRQA 107



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>SMT1_ARATH (Q9LM02) Cycloartenol-C-24-methyltransferase (EC 2.1.1.41)|
           (24-sterol C-methyltransferase 1) (Sterol
           C-methyltransferase 1) (STEROL METHYLTRANSFERASE 1)
           (CEPHALOPOD protein)
          Length = 336

 Score = 44.3 bits (103), Expect = 9e-05
 Identities = 22/63 (34%), Positives = 33/63 (52%)
 Frame = +1

Query: 52  DKYTQYWSFFRPPQETATTQASADQVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSH 231
           +KY QY  F    +E      +       V+ +Y+L T  YE+GWG+SFHF+    G S 
Sbjct: 21  EKYEQYHVFHGGNEEERKANYTD-----MVNKYYDLATSFYEYGWGESFHFAQRWKGESL 75

Query: 232 RDA 240
           R++
Sbjct: 76  RES 78



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>ERG6_KLULA (Q6CYB3) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 371

 Score = 44.3 bits (103), Expect = 9e-05
 Identities = 17/28 (60%), Positives = 21/28 (75%)
 Frame = +1

Query: 145 TFYNLVTDIYEWGWGQSFHFSPSLPGRS 228
           ++YN+VTD YE+GWG SFHFS    G S
Sbjct: 72  SYYNVVTDFYEYGWGSSFHFSRFFKGES 99



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>ERG6_YEAST (P25087) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 382

 Score = 43.9 bits (102), Expect = 1e-04
 Identities = 17/28 (60%), Positives = 21/28 (75%)
 Frame = +1

Query: 145 TFYNLVTDIYEWGWGQSFHFSPSLPGRS 228
           ++YN+VTD YE+GWG SFHFS    G S
Sbjct: 71  SYYNVVTDFYEYGWGSSFHFSRFYKGES 98



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>ERG6_ASHGO (Q759S7) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 373

 Score = 43.5 bits (101), Expect = 1e-04
 Identities = 17/28 (60%), Positives = 21/28 (75%)
 Frame = +1

Query: 145 TFYNLVTDIYEWGWGQSFHFSPSLPGRS 228
           ++YN+VTD YE+GWG SFHFS    G S
Sbjct: 73  SYYNVVTDFYEYGWGASFHFSRFYTGES 100



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>ERG6_CANGA (Q6FRZ7) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 372

 Score = 43.5 bits (101), Expect = 1e-04
 Identities = 17/32 (53%), Positives = 23/32 (71%)
 Frame = +1

Query: 145 TFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDA 240
           ++YN+VTD YE+GWG SFHFS    G + + A
Sbjct: 72  SYYNVVTDFYEYGWGSSFHFSRFYKGENFQAA 103



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>ERG6_PNECA (Q96WX4) Sterol 24-C-methyltransferase (EC 2.1.1.41)|
           (Delta(24)-sterol C-methyltransferase)
          Length = 377

 Score = 38.9 bits (89), Expect = 0.004
 Identities = 14/19 (73%), Positives = 16/19 (84%)
 Frame = +1

Query: 148 FYNLVTDIYEWGWGQSFHF 204
           +YNLVTD YE+GW  SFHF
Sbjct: 80  YYNLVTDFYEYGWSTSFHF 98



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>RAD5_DEBHA (Q6BIP2) DNA repair protein RAD5 (EC 3.6.1.-)|
          Length = 1153

 Score = 30.0 bits (66), Expect = 1.7
 Identities = 18/45 (40%), Positives = 22/45 (48%)
 Frame = +1

Query: 103 TTQASADQVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRD 237
           TTQ  A+ +    D F N + DI+EW    S H S SLP     D
Sbjct: 470 TTQDKAN-IQERNDAFMNPLWDIFEWPKDTSMHKSESLPTEDRMD 513



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>AREA_GIBFU (P78688) Nitrogen regulatory protein areA|
          Length = 971

 Score = 28.5 bits (62), Expect = 4.8
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +1

Query: 70  WSFFRPPQETATTQASADQVPAFVDTFYNLVTDIYEWGWGQSFHFSPS 213
           +S  +P Q     Q++   VP  +DTF    + +    + Q+F FSPS
Sbjct: 322 YSLDQPNQAGIPQQSNGSNVPFNIDTFMENDSMVNNGNFQQNFSFSPS 369



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>AMPM1_SCHPO (O59730) Probable methionine aminopeptidase 1 (EC 3.4.11.18) (MetAP|
           1) (MAP 1) (Peptidase M 1)
          Length = 379

 Score = 27.7 bits (60), Expect = 8.2
 Identities = 12/21 (57%), Positives = 13/21 (61%)
 Frame = +1

Query: 181 GWGQSFHFSPSLPGRSHRDAP 243
           G  Q FH SPS+P  SH  AP
Sbjct: 290 GINQLFHCSPSIPHYSHNKAP 310


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,512,417
Number of Sequences: 219361
Number of extensions: 298744
Number of successful extensions: 1195
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1193
length of database: 80,573,946
effective HSP length: 56
effective length of database: 68,289,730
effective search space used: 1638953520
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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