ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart35e05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1ZRP4_MAIZE (P47917) O-methyltransferase ZRP4 (EC 2.1.1.-) (OMT) 82 8e-16
2CVMT1_OCIBA (Q93WU3) Chavicol O-methyltransferase (EC 2.1.1.146)... 77 3e-14
3EOMT1_OCIBA (Q93WU2) Eugenol O-methyltransferase (EC 2.1.1.146) ... 70 3e-12
47OMT9_MEDSA (O22309) Isoflavone-7-O-methytransferase 9 (EC 2.1.1... 60 4e-09
57OMT8_MEDSA (O24529) Isoflavone-7-O-methytransferase 8 (EC 2.1.1... 60 5e-09
67OMT6_MEDSA (O22308) Isoflavone-7-O-methytransferase 6 (EC 2.1.1... 60 5e-09
76OMT_COPJA (Q9LEL6) (RS)-norcoclaurine 6-O-methyltransferase (EC... 54 3e-07
8COOMT_COPJA (Q8H9A8) Columbamine O-methyltransferase (EC 2.1.1.1... 50 5e-06
94OMT_COPJA (Q9LEL5) 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-meth... 44 4e-04
10SPTA2_CHICK (P07751) Spectrin alpha chain, brain (Spectrin, non-... 32 1.2
11K1C4_XENLA (P05781) Keratin, type I cytoskeletal 47 kDa 30 4.5
12K1C12_HUMAN (Q99456) Keratin, type I cytoskeletal 12 (Cytokerati... 30 4.5
13SPTA2_RAT (P16086) Spectrin alpha chain, brain (Spectrin, non-er... 30 5.9
14EPN4_MOUSE (Q99KN9) Epsin-4 (Epsin-related protein) (EpsinR) (En... 29 10.0
15PUR6_PYRAB (Q9UY68) Phosphoribosylaminoimidazole carboxylase cat... 29 10.0
16SMT_COPJA (Q39522) (S)-scoulerine 9-O-methyltransferase (EC 2.1.... 29 10.0
17SPTA2_HUMAN (Q13813) Spectrin alpha chain, brain (Spectrin, non-... 29 10.0

>ZRP4_MAIZE (P47917) O-methyltransferase ZRP4 (EC 2.1.1.-) (OMT)|
          Length = 364

 Score = 82.4 bits (202), Expect = 8e-16
 Identities = 54/154 (35%), Positives = 74/154 (48%), Gaps = 5/154 (3%)
 Frame = +2

Query: 77  TDAELLQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDLMAALSLPPVKR 256
           TD  LL AQ +LW  +  ++ SMAL+ A+ L I  AIH  GGAASL  +++ + L P + 
Sbjct: 9   TDQSLLDAQLELWHTTFAFMKSMALKSAIHLRIADAIHLHGGAASLSQILSKVHLHPSRV 68

Query: 257 PFLRRVMRVLVTSGAFXX-----XXXXXXXXIYRLTPLSRILVHGVVADEHHSQKYFVLG 421
             LRR+MRVL T+  F               +Y LTP+SR+L+         +Q      
Sbjct: 69  SSLRRLMRVLTTTNVFGTQPLGGGSDDDSEPVYTLTPVSRLLIGS--QSSQLAQTPLAAM 126

Query: 422 VTSPHYTEAAMGLAGWFRKDHEPPVPSPFEDIFG 523
           V  P        L  WF+  HE P P  F+   G
Sbjct: 127 VLDPTIVSPFSELGAWFQ--HELPDPCIFKHTHG 158



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>CVMT1_OCIBA (Q93WU3) Chavicol O-methyltransferase (EC 2.1.1.146) ((Iso)eugenol|
           O-methyltransferase CVOMT1)
           (S-adenosysl-L-methionine:(Iso)eugenol
           O-methyltransferase CVOMT1)
          Length = 356

 Score = 77.0 bits (188), Expect = 3e-14
 Identities = 55/182 (30%), Positives = 84/182 (46%), Gaps = 15/182 (8%)
 Frame = +2

Query: 44  MAAQAQTIEVPTDAELLQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDL 223
           MA Q   I + T+ +LLQAQA +W H   + +SM+L+CA++L IP  +H+     +L  L
Sbjct: 1   MALQNMDISLSTE-QLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKHDHPMTLSQL 59

Query: 224 MAALSLPPVKRPFLRRVMRVLVTSGAFXXXXXXXXXXIYRLTPLSRILVHG--------- 376
           + A+ +   K    +R+MR LV S  F           Y LTP SR+L+ G         
Sbjct: 60  LKAIPINKEKSQSFQRLMRALVNSNFFIEENSNNQEVCYWLTPASRLLLKGAPLTVAPLV 119

Query: 377 -VVADEHHSQKYFVLGVTSPHYT-----EAAMGLAGWFRKDHEPPVPSPFEDIFGVPLCD 538
            VV D   +  +  +     H       EAA G   W +  ++P +   F++      CD
Sbjct: 120 QVVLDPTFTNPWHYMSEWFKHENHATQFEAANGCTFWEKLANKPSMGRFFDEAMS---CD 176

Query: 539 DR 544
            R
Sbjct: 177 SR 178



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>EOMT1_OCIBA (Q93WU2) Eugenol O-methyltransferase (EC 2.1.1.146) ((Iso)eugenol|
           O-methyltransferase EOMT1)
           (S-adenosysl-L-methionine:(Iso)eugenol
           O-methyltransferase EOMT1)
          Length = 357

 Score = 70.5 bits (171), Expect = 3e-12
 Identities = 55/184 (29%), Positives = 85/184 (46%), Gaps = 17/184 (9%)
 Frame = +2

Query: 44  MAAQAQTIEVPTDAELLQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDL 223
           MA Q   I + T+ +LLQAQ  +W H   + +SM+L+CA++L IP  +H+ G   +L  L
Sbjct: 1   MALQKVDISLSTE-QLLQAQVHVWNHMYAFANSMSLKCAIQLGIPDILHKHGRPMTLSQL 59

Query: 224 MAALSLPPVKRPFLRRVMRVLVTSGAFXXXXXXXXXXI-YRLTPLSRILVH--------- 373
           + ++ +   K    +R+MR LV S  F          + Y LTP S +L+          
Sbjct: 60  LQSIPINKEKTQCFQRLMRALVNSNFFIEENNSNNQEVCYWLTPASCLLLKEAPLTVTPL 119

Query: 374 -GVVADE------HHSQKYFVLGVTSPHYTEAAMGLAGWFRKDHEPPVPSPFEDIFGVPL 532
             VV D       HH  ++F     +  + EAA G   W +  +EP     F++      
Sbjct: 120 VQVVLDPTFTNPWHHMSEWFTHEKHATQF-EAANGCTFWEKLANEPSKGRFFDEAMS--- 175

Query: 533 CDDR 544
           CD R
Sbjct: 176 CDSR 179



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>7OMT9_MEDSA (O22309) Isoflavone-7-O-methytransferase 9 (EC 2.1.1.150)|
           (Isoflavone-O-methytransferase 9) (7 IOMT-9)
          Length = 352

 Score = 60.1 bits (144), Expect = 4e-09
 Identities = 38/107 (35%), Positives = 57/107 (53%)
 Frame = +2

Query: 56  AQTIEVPTDAELLQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDLMAAL 235
           A +I     +E+ +AQA L++H   ++ SM+L+ AV + IP  IH  G   SL +L++ L
Sbjct: 2   ASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHGKPISLSNLVSIL 61

Query: 236 SLPPVKRPFLRRVMRVLVTSGAFXXXXXXXXXXIYRLTPLSRILVHG 376
            +P  K   +RR+MR L  +G F           Y LT  S +LV G
Sbjct: 62  QVPSSKIGNVRRLMRYLAHNGFFEIITKEEES--YALTVASELLVRG 106



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>7OMT8_MEDSA (O24529) Isoflavone-7-O-methytransferase 8 (EC 2.1.1.150)|
           (Isoflavone-O-methytransferase 8) (7-IOMT-8)
          Length = 352

 Score = 59.7 bits (143), Expect = 5e-09
 Identities = 38/107 (35%), Positives = 57/107 (53%)
 Frame = +2

Query: 56  AQTIEVPTDAELLQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDLMAAL 235
           A +I     +E+ +AQA L++H   ++ SM+L+ AVE+ IP  I   G   SL +L++ L
Sbjct: 2   ASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSIL 61

Query: 236 SLPPVKRPFLRRVMRVLVTSGAFXXXXXXXXXXIYRLTPLSRILVHG 376
            +P  K   +RR+MR L  +G F           Y LT  S +LV G
Sbjct: 62  QVPSSKIGNVRRLMRYLAHNGFFEIITKEEES--YALTVASELLVRG 106



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>7OMT6_MEDSA (O22308) Isoflavone-7-O-methytransferase 6 (EC 2.1.1.150)|
           (Isoflavone-O-methytransferase 6) (7-IOMT-6)
          Length = 352

 Score = 59.7 bits (143), Expect = 5e-09
 Identities = 38/107 (35%), Positives = 57/107 (53%)
 Frame = +2

Query: 56  AQTIEVPTDAELLQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDLMAAL 235
           A +I     +E+ +AQA L++H   ++ SM+L+ AVE+ IP  I   G   SL +L++ L
Sbjct: 2   ASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSIL 61

Query: 236 SLPPVKRPFLRRVMRVLVTSGAFXXXXXXXXXXIYRLTPLSRILVHG 376
            +P  K   +RR+MR L  +G F           Y LT  S +LV G
Sbjct: 62  QVPSSKIGNVRRLMRYLAHNGFFEIITKEEES--YALTVASELLVRG 106



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>6OMT_COPJA (Q9LEL6) (RS)-norcoclaurine 6-O-methyltransferase (EC 2.1.1.128)|
           (S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase) (6-OMT)
          Length = 347

 Score = 53.9 bits (128), Expect = 3e-07
 Identities = 33/95 (34%), Positives = 46/95 (48%)
 Frame = +2

Query: 92  LQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDLMAALSLPPVKRPFLRR 271
           L +QA LW     +  S+ L+CAV+L++   IH  G + +L +L + L   PV    L R
Sbjct: 8   LSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNEDALYR 67

Query: 272 VMRVLVTSGAFXXXXXXXXXXIYRLTPLSRILVHG 376
           VMR LV    F           Y L P ++ LV G
Sbjct: 68  VMRYLVHMKLFTKASIDGELR-YGLAPPAKYLVKG 101



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>COOMT_COPJA (Q8H9A8) Columbamine O-methyltransferase (EC 2.1.1.118) (CoOMT)|
          Length = 351

 Score = 49.7 bits (117), Expect = 5e-06
 Identities = 32/96 (33%), Positives = 43/96 (44%), Gaps = 1/96 (1%)
 Frame = +2

Query: 92  LQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDLMAALSLPPVKRPFLRR 271
           ++AQA +W+H   +  ++ LR  V L IP  IH   G  +L  L+  L L          
Sbjct: 13  IKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHN-NGPVTLSQLVTHLPLKSTSIDRFHH 71

Query: 272 VMRVLVTSGAFX-XXXXXXXXXIYRLTPLSRILVHG 376
            MR LV    F            Y LTP S++LVHG
Sbjct: 72  FMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHG 107



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>4OMT_COPJA (Q9LEL5) 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase|
           (EC 2.1.1.116)
           (S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase) (4'-OMT)
          Length = 350

 Score = 43.5 bits (101), Expect = 4e-04
 Identities = 32/116 (27%), Positives = 50/116 (43%)
 Frame = +2

Query: 92  LQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAASLPDLMAALSLPPVKRPFLRR 271
           ++AQA +W+    +  S+ LRCAVEL I   I       +L DL + L +  V    L R
Sbjct: 12  IKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLYR 71

Query: 272 VMRVLVTSGAFXXXXXXXXXXIYRLTPLSRILVHGVVADEHHSQKYFVLGVTSPHY 439
           ++R LV                Y L P++ +L      +   S    +LG+T   +
Sbjct: 72  ILRYLVKMEILRVEKSDDGQKKYALEPIATLLSR----NAKRSMVPMILGMTQKDF 123



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>SPTA2_CHICK (P07751) Spectrin alpha chain, brain (Spectrin, non-erythroid alpha|
           chain) (Fodrin alpha chain)
          Length = 2477

 Score = 32.0 bits (71), Expect = 1.2
 Identities = 18/59 (30%), Positives = 30/59 (50%)
 Frame = -1

Query: 529 RHAEDVLEWRRHRRLMVFPEPAGQPHRRLRVMRRGHAEHKILLAMMLIRYDTVHQDAGQ 353
           R  EDV++W   +  +V  E  GQ    + V+++   E +  LA    R + V+Q AG+
Sbjct: 156 RECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAGK 214



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>K1C4_XENLA (P05781) Keratin, type I cytoskeletal 47 kDa|
          Length = 419

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
 Frame = +2

Query: 38  GAMAAQAQTIEVPT---DAELLQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGG 202
           G   AQ QTI+      + +LLQ ++D+ R ++ Y   + ++  +E+EI T    L G
Sbjct: 335 GRYGAQLQTIQFSLRSLEEQLLQIRSDMERQNMEYRQLLDIKTRLEMEIETYRRLLEG 392



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>K1C12_HUMAN (Q99456) Keratin, type I cytoskeletal 12 (Cytokeratin-12) (CK-12)|
           (Keratin-12) (K12)
          Length = 494

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 18/56 (32%), Positives = 30/56 (53%)
 Frame = +2

Query: 41  AMAAQAQTIEVPTDAELLQAQADLWRHSLYYLSSMALRCAVELEIPTAIHRLGGAA 208
           A  +Q Q +    +A+LLQ +AD  R ++ +   + ++  +ELEI T    L G A
Sbjct: 382 AQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEA 437



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>SPTA2_RAT (P16086) Spectrin alpha chain, brain (Spectrin, non-erythroid alpha|
           chain) (Alpha-II spectrin) (Fodrin alpha chain)
          Length = 2472

 Score = 29.6 bits (65), Expect = 5.9
 Identities = 17/59 (28%), Positives = 29/59 (49%)
 Frame = -1

Query: 529 RHAEDVLEWRRHRRLMVFPEPAGQPHRRLRVMRRGHAEHKILLAMMLIRYDTVHQDAGQ 353
           R  EDV++W   +  +V  E  GQ    + V+++   E +  LA    R + V+Q A +
Sbjct: 156 RECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQFAAK 214



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>EPN4_MOUSE (Q99KN9) Epsin-4 (Epsin-related protein) (EpsinR) (Enthoprotin)|
          Length = 631

 Score = 28.9 bits (63), Expect = 10.0
 Identities = 12/36 (33%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
 Frame = -2

Query: 549 GVLSSQSGTPKMSSNGDGTGGSWSFLNQ-PASPIAA 445
           G  ++    P  +++G+G  G WS  NQ P+ P+A+
Sbjct: 359 GSAAASGNFPSQATSGNGDFGDWSAFNQAPSGPVAS 394



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>PUR6_PYRAB (Q9UY68) Phosphoribosylaminoimidazole carboxylase catalytic subunit|
           (EC 4.1.1.21) (AIR carboxylase) (AIRC)
          Length = 174

 Score = 28.9 bits (63), Expect = 10.0
 Identities = 16/33 (48%), Positives = 20/33 (60%)
 Frame = +2

Query: 152 RCAVELEIPTAIHRLGGAASLPDLMAALSLPPV 250
           R A E  I   I   GGAA LP ++AAL++ PV
Sbjct: 55  RTAEERGIEVIIAGAGGAAHLPGVLAALTMIPV 87



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>SMT_COPJA (Q39522) (S)-scoulerine 9-O-methyltransferase (EC 2.1.1.117)|
          Length = 381

 Score = 28.9 bits (63), Expect = 10.0
 Identities = 27/82 (32%), Positives = 38/82 (46%), Gaps = 6/82 (7%)
 Frame = +2

Query: 143 MALRCAVELEIPTAIHRLGGAASL--PDLMAALSLPPVKRP----FLRRVMRVLVTSGAF 304
           MALR A+EL +   I + G  A L   D++A +   P K P     L R++R+L  S + 
Sbjct: 53  MALRAAIELNVFEIISQAGPDAQLSPSDIVAKI---PTKNPSAAISLDRILRMLGAS-SI 108

Query: 305 XXXXXXXXXXIYRLTPLSRILV 370
                     +Y L   SR LV
Sbjct: 109 LSVSTTKSGRVYGLNEESRCLV 130



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>SPTA2_HUMAN (Q13813) Spectrin alpha chain, brain (Spectrin, non-erythroid alpha|
           chain) (Alpha-II spectrin) (Fodrin alpha chain)
          Length = 2472

 Score = 28.9 bits (63), Expect = 10.0
 Identities = 16/59 (27%), Positives = 29/59 (49%)
 Frame = -1

Query: 529 RHAEDVLEWRRHRRLMVFPEPAGQPHRRLRVMRRGHAEHKILLAMMLIRYDTVHQDAGQ 353
           R  EDV++W   +  +V  E  GQ    + V+++   E +  +A    R + V+Q A +
Sbjct: 156 RECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAK 214


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,040,235
Number of Sequences: 219361
Number of extensions: 1292765
Number of successful extensions: 3927
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 3791
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3925
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4430660157
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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