ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart28g01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1YLE1_SCHPO (Q10451) Hypothetical protein C1093.01 in chromosome I 45 1e-04
2RF1_ORYSA (Q76C99) Rf1 protein, mitochondrial precursor (PPR pro... 39 0.013
3Y4791_ARATH (P0C043) Hypothetical PPR-repeat protein At4g17915 36 0.083
4PQE1_CAEEL (Q10124) Putative RNA exonuclease pqe-1 (PolyQ enhanc... 32 1.2
5AFSR_STRCO (P25941) Regulatory protein afsR 30 3.5
6ECTC_VIBCH (Q9KLC3) L-ectoine synthase (EC 4.2.1.-) (N-acetyldia... 30 4.5
7RUVB_SYNS9 (Q3B0J1) Holliday junction ATP-dependent DNA helicase... 30 5.9
8PDXJ_GLUOX (Q5FNS2) Pyridoxal phosphate biosynthetic protein pdx... 30 5.9
9POLN_RUBVM (Q86500) Nonstructural polyprotein [Contains: Proteas... 29 7.8

>YLE1_SCHPO (Q10451) Hypothetical protein C1093.01 in chromosome I|
          Length = 1261

 Score = 45.4 bits (106), Expect = 1e-04
 Identities = 33/96 (34%), Positives = 46/96 (47%)
 Frame = -1

Query: 530  RNGRGEDAVRAFRDMAETSKKRNEVKPDSVTFVAVLSACSHSGLVAEGRELFAEMLPAHG 351
            R G  +DA  A     ET  KR+ VKP    + AVLS    +    E  +LF EM  + G
Sbjct: 900  RRGDTDDATTALNIFEET--KRHNVKPSVFLYNAVLSKLGRARRTTECWKLFQEMKES-G 956

Query: 350  VAPGAEHYGCMVDLLCRAGLLGEAVRLVRTMPVRPN 243
            + P +  YG +++  CR G    A +L   M  +PN
Sbjct: 957  LLPTSVTYGTVINAACRIGDESLAEKLFAEMENQPN 992



 Score = 33.9 bits (76), Expect = 0.31
 Identities = 23/78 (29%), Positives = 37/78 (47%)
 Frame = -1

Query: 548  VIAGLGRNGRGEDAVRAFRDMAETSKKRNEVKPDSVTFVAVLSACSHSGLVAEGRELFAE 369
            V++ LGR  R  +  + F++M E+      + P SVT+  V++A    G  +   +LFAE
Sbjct: 932  VLSKLGRARRTTECWKLFQEMKESG-----LLPTSVTYGTVINAACRIGDESLAEKLFAE 986

Query: 368  MLPAHGVAPGAEHYGCMV 315
            M       P    Y  M+
Sbjct: 987  MENQPNYQPRVAPYNTMI 1004



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>RF1_ORYSA (Q76C99) Rf1 protein, mitochondrial precursor (PPR protein)|
           (Fertility restorer) (Restorer for CMS)
          Length = 791

 Score = 38.5 bits (88), Expect = 0.013
 Identities = 19/64 (29%), Positives = 36/64 (56%)
 Frame = -1

Query: 467 RNEVKPDSVTFVAVLSACSHSGLVAEGRELFAEMLPAHGVAPGAEHYGCMVDLLCRAGLL 288
           RN + PD   F  ++ A +  G V +   +F++M    G+ P A  YG ++ +LC++G +
Sbjct: 364 RNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQ-QGLNPNAVTYGAVIGILCKSGRV 422

Query: 287 GEAV 276
            +A+
Sbjct: 423 EDAM 426



 Score = 36.2 bits (82), Expect = 0.063
 Identities = 35/169 (20%), Positives = 70/169 (41%), Gaps = 17/169 (10%)
 Frame = -1

Query: 551  TVIAGLGRNGRGEDAVRAFRDMAETSKKRNEVKPDSVTFVAVLSACSHSGLVAEGRELFA 372
            T+I G  +  R EDA+  F++M  +      V PD +T+  +L     +   A  +EL+ 
Sbjct: 551  TLINGYCKISRMEDALVLFKEMESSG-----VSPDIITYNIILQGLFQTRRTAAAKELYV 605

Query: 371  EMLPAHGVAPGAEHYGCMVDLLCRAGLLGEAVRLVRT---MPVRPNAKVLGCLL------ 219
             +  + G       Y  ++  LC+  L  +A+++ +    M ++  A+    ++      
Sbjct: 606  RITES-GTQIELSTYNIILHGLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKV 664

Query: 218  --------LHARSSGDGVAASEWAAQRIAELDDRDGAAYGLSNLYASLQ 96
                    L    S +G+  + W  + +AE     G    L  L+ S++
Sbjct: 665  GRNDEAKDLFVAFSSNGLVPNYWTYRLMAENIIGQGLLEELDQLFLSME 713



 Score = 35.4 bits (80), Expect = 0.11
 Identities = 24/97 (24%), Positives = 44/97 (45%)
 Frame = -1

Query: 548 VIAGLGRNGRGEDAVRAFRDMAETSKKRNEVKPDSVTFVAVLSACSHSGLVAEGRELFAE 369
           +I    + G+ + A+  F  M     ++  + P++VT+ AV+     SG V +    F +
Sbjct: 377 LICAYAKQGKVDQAMLVFSKM-----RQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQ 431

Query: 368 MLPAHGVAPGAEHYGCMVDLLCRAGLLGEAVRLVRTM 258
           M+   G++PG   Y  ++  LC       A  L+  M
Sbjct: 432 MID-EGLSPGNIVYNSLIHGLCTCNKWERAEELILEM 467



 Score = 30.8 bits (68), Expect = 2.7
 Identities = 22/98 (22%), Positives = 46/98 (46%)
 Frame = -1

Query: 551 TVIAGLGRNGRGEDAVRAFRDMAETSKKRNEVKPDSVTFVAVLSACSHSGLVAEGRELFA 372
           +++ G   +G+ ++A+   + M     + + V+PD VT+  ++     +G   E R++F 
Sbjct: 271 SILHGYCSSGQPKEAIGFLKKM-----RSDGVEPDVVTYSLLMDYLCKNGRCMEARKIF- 324

Query: 371 EMLPAHGVAPGAEHYGCMVDLLCRAGLLGEAVRLVRTM 258
           + +   G+ P    YG ++      G L E   L+  M
Sbjct: 325 DSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLM 362



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>Y4791_ARATH (P0C043) Hypothetical PPR-repeat protein At4g17915|
          Length = 463

 Score = 35.8 bits (81), Expect = 0.083
 Identities = 27/127 (21%), Positives = 55/127 (43%), Gaps = 1/127 (0%)
 Frame = -1

Query: 551 TVIAGLGRNGRGEDAVRAFRDMAETSKKRNEVKPDSVTFVAVLSACSHSGLVAEGRELFA 372
           ++IAG  R    +  +  F +M E       + PD  ++  ++      G   E   +  
Sbjct: 88  SLIAGAARRLMLDHVLYLFDEMLEWG-----IYPDLWSYNTLMCCYFKLGKHEEAFRVLY 142

Query: 371 EMLPAHGVAPGAEHYGCMVDLLCRAGLLGEAVRLVRTMPVRPNAKVLGC-LLLHARSSGD 195
           + L   G+ PG + Y  ++D LC+ G +  A+ L + M  R   +++   +L++      
Sbjct: 143 KDLQLAGLNPGPDTYNVLLDALCKCGYIDNALELFKEMQSRFKPELMTYNILINGLCKSR 202

Query: 194 GVAASEW 174
            V  ++W
Sbjct: 203 RVGTAKW 209



 Score = 33.9 bits (76), Expect = 0.31
 Identities = 20/74 (27%), Positives = 33/74 (44%)
 Frame = -1

Query: 470 KRNEVKPDSVTFVAVLSACSHSGLVAEGRELFAEMLPAHGVAPGAEHYGCMVDLLCRAGL 291
           +R  +K D  T   +++    +G      E F  M    G+        C+VD LC+AG 
Sbjct: 320 ERRGMKADEYTHTIIVNGLLRTGQTRRAEEHFVSM-GEMGIGLNLVTCNCLVDGLCKAGH 378

Query: 290 LGEAVRLVRTMPVR 249
           +  A+R   +M V+
Sbjct: 379 VDRAMRYFESMEVK 392



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>PQE1_CAEEL (Q10124) Putative RNA exonuclease pqe-1 (PolyQ enhancer protein 1)|
          Length = 1647

 Score = 32.0 bits (71), Expect = 1.2
 Identities = 36/144 (25%), Positives = 50/144 (34%), Gaps = 10/144 (6%)
 Frame = +3

Query: 21  PARLLAARHGRSHQPAPVPLDV-IPPLQRRVQVGQPVRGAVAVVQXXXXXXXXXXXXXAV 197
           PA    A++ +   P  VP+   + P     Q   P   A  + Q             A 
Sbjct: 281 PANAQLAQNAQQRNPQQVPMPQGVAPHHIPPQALGPHGAAPQMTQQAPARGPSGAAQHAQ 340

Query: 198 PRAARVEQEAPQDLSV-GPDGHRPHE-----PHGLAEQPRXXXXXXXXXXXLRPGSHPVG 359
                  Q+ P    V GP    P       PHG A+ P            L PG+HP+ 
Sbjct: 341 NAHRMTHQQVPLQAPVQGPHRGAPQMAQQPGPHGAAQHPSSGTVAPMHITSL-PGNHPLN 399

Query: 360 RQHL---GEELPALRNQAGVAARR 422
           R HL    + +PAL  +  +A  R
Sbjct: 400 RTHLLFNRQVVPALDIRNLIAQHR 423



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>AFSR_STRCO (P25941) Regulatory protein afsR|
          Length = 993

 Score = 30.4 bits (67), Expect = 3.5
 Identities = 32/144 (22%), Positives = 52/144 (36%)
 Frame = -1

Query: 542  AGLGRNGRGEDAVRAFRDMAETSKKRNEVKPDSVTFVAVLSACSHSGLVAEGRELFAEML 363
            AG  R  R  D + A +D+ E+    ++ +  +        + + +      R + +++L
Sbjct: 694  AGTARLRRAVDLLWAAKDLTESGANSHQYEATARAMCDATGSAADTRAEGRARTVLSDVL 753

Query: 362  PAHGVAPGAEHYGCMVDLLCRAGLLGEAVRLVRTMPVRPNAKVLGCLLLHARSSGDGVAA 183
               G    AE    +   L  +     AV  V        A   G + LH R   +G   
Sbjct: 754  LVSGRIEHAEEEARLAMRLAGSAEDSAAVSWV--------ANNRGLVCLHQRRYAEGKGL 805

Query: 182  SEWAAQRIAELDDRDGAAYGLSNL 111
               A       D+R G A  LSNL
Sbjct: 806  FHQAIAGFRATDNRAGEASALSNL 829



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>ECTC_VIBCH (Q9KLC3) L-ectoine synthase (EC 4.2.1.-) (N-acetyldiaminobutyrate|
           dehydratase)
          Length = 138

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 15/50 (30%), Positives = 23/50 (46%)
 Frame = -1

Query: 221 LLHARSSGDGVAASEWAAQRIAELDDRDGAAYGLSNLYASLQRWDHVERH 72
           L   R S   V A  W + R+   DD  G ++ ++ +YA+ Q   H   H
Sbjct: 6   LEECRQSERRVVAENWESVRMLLKDDHMGFSFHITTIYANTQTHIHYRNH 55



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>RUVB_SYNS9 (Q3B0J1) Holliday junction ATP-dependent DNA helicase ruvB (EC|
           3.6.1.-)
          Length = 347

 Score = 29.6 bits (65), Expect = 5.9
 Identities = 33/118 (27%), Positives = 45/118 (38%)
 Frame = -1

Query: 506 VRAFRDMAETSKKRNEVKPDSVTFVAVLSACSHSGLVAEGRELFAEMLPAHGVAPGAEHY 327
           +R  RD+A   + +  +    V     L    H GL A  R L A ++  HG  P     
Sbjct: 241 LRRVRDVASVRQGKGTIDDAMVAEALSLHRVDHRGLDASDRRLLAMLMDQHGGGP----- 295

Query: 326 GCMVDLLCRAGLLGEAVRLVRTMPVRPNAKVLGCLLLHARSSGDGVAASEWAAQRIAE 153
              V L   A  LGE    + T+ V P     G L+   R    G   ++ A   IAE
Sbjct: 296 ---VGLETLAAALGEDPVTLETV-VEPFLLQQGLLVRTPR----GRMVTDAARSHIAE 345



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>PDXJ_GLUOX (Q5FNS2) Pyridoxal phosphate biosynthetic protein pdxJ (PNP|
           synthase)
          Length = 232

 Score = 29.6 bits (65), Expect = 5.9
 Identities = 22/56 (39%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
 Frame = +3

Query: 333 LRPGSHPVGRQHLGEELPALRNQAG-VAARRQDRHERHGVRLDLVSLLARLRHVAE 497
           L  G++ +G     EEL  LR+ AG VAA   + H  HG+  D V  +A L  +AE
Sbjct: 151 LHTGAYALGGS---EELERLRSAAGTVAACGLELHAGHGLTYDNVGAIADLTGLAE 203



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>POLN_RUBVM (Q86500) Nonstructural polyprotein [Contains: Protease (EC|
           3.4.22.-) (p150); RNA-directed RNA polymerase/helicase
           (EC 2.7.7.48) (EC 3.6.1.-) (p90)]
          Length = 2115

 Score = 29.3 bits (64), Expect = 7.8
 Identities = 23/73 (31%), Positives = 27/73 (36%)
 Frame = +1

Query: 325 P*CSAPGATPWAGSISAKSSRPSATRPEWLHADRTATNVTESGLTSFLFLLVSAMSRNAL 504
           P C  P   P   S  A      A  P WL A+R A    E            A+   A 
Sbjct: 499 PRCDVPRERP---SAPAGPPDDEALIPPWLFAERRALRCREWDF--------EALRARAD 547

Query: 505 TASSPRPFLPSPA 543
           TA++P P  P PA
Sbjct: 548 TAAAPAPLAPRPA 560


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,150,363
Number of Sequences: 219361
Number of extensions: 1109904
Number of successful extensions: 4704
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4376
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4699
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4488201198
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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