ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart28b04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1EI2BA_SCHPO (Q9USP0) Translation initiation factor eIF-2B alpha ... 64 3e-10
2EI2BA_YEAST (P14741) Translation initiation factor eIF-2B alpha ... 62 1e-09
3EI2BA_RAT (Q64270) Translation initiation factor eIF-2B alpha su... 54 3e-07
4EI2BA_MACFA (Q4R4V8) Translation initiation factor eIF-2B alpha ... 54 3e-07
5EI2BA_HUMAN (Q14232) Translation initiation factor eIF-2B alpha ... 52 1e-06
6EI2BA_MOUSE (Q99LC8) Translation initiation factor eIF-2B alpha ... 50 4e-06
7EI2BA_CAEEL (P34604) Putative translation initiation factor eIF-... 40 0.005
8EI2B2_METJA (Q57586) Putative translation initiation factor eIF-... 35 0.12
9EI2B1_METJA (Q57896) Putative translation initiation factor eIF-... 31 3.0
10KTHY_CHLPN (Q9Z8R5) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase) 29 8.8
11SEP15_HUMAN (O60613) 15 kDa selenoprotein precursor 29 8.8

>EI2BA_SCHPO (Q9USP0) Translation initiation factor eIF-2B alpha subunit (eIF-2B|
           GDP-GTP exchange factor)
          Length = 341

 Score = 63.9 bits (154), Expect = 3e-10
 Identities = 38/94 (40%), Positives = 53/94 (56%)
 Frame = +1

Query: 307 SRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLI 486
           S+A T+ E    L+  S+ LK     +ISLSA CD+F RFVTR+ H +   F+  K  L+
Sbjct: 50  SQAKTISEFMDILQNGSNTLKEGVQNNISLSAGCDIFQRFVTRSLH-DVGDFEQCKRHLV 108

Query: 487 ERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588
           E G+ F + +   R+ IA L    I DGS +L H
Sbjct: 109 ENGKLFIQRARACRQRIAHLGYPLIRDGSVILTH 142



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>EI2BA_YEAST (P14741) Translation initiation factor eIF-2B alpha subunit (eIF-2B|
           GDP-GTP exchange factor) (Guanine nucleotide exchange
           factor subunit GCN3) (GCD complex subunit GCN3)
           (Transcriptional activator GCN3)
          Length = 305

 Score = 62.0 bits (149), Expect = 1e-09
 Identities = 35/90 (38%), Positives = 52/90 (57%)
 Frame = +1

Query: 319 TMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLIERGE 498
           T  E+   +K ++++L      S+SL A CD+FMRFV R  HL +  ++  K  LIE G+
Sbjct: 41  TAAEMINTIKSSTEELIKSIPNSVSLRAGCDIFMRFVLRNLHL-YGDWENCKQHLIENGQ 99

Query: 499 KFGEISLKARKTIAMLSQDFISDGSTMLVH 588
            F   + K+R  IA +  DFI+D   +LVH
Sbjct: 100 LFVSRAKKSRNKIAEIGVDFIADDDIILVH 129



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>EI2BA_RAT (Q64270) Translation initiation factor eIF-2B alpha subunit (eIF-2B|
           GDP-GTP exchange factor)
          Length = 305

 Score = 54.3 bits (129), Expect = 3e-07
 Identities = 37/97 (38%), Positives = 57/97 (58%), Gaps = 1/97 (1%)
 Frame = +1

Query: 301 RSSRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSR 480
           +  +  T+  L   L KA + L + D+ S+++S+  +LF+RF++ TS LE+  +   K  
Sbjct: 35  KRDKGETIQGLRAHLTKAIETLCAVDS-SVAVSSGGELFLRFISLTS-LEYSDYSKCKKI 92

Query: 481 LIERGEKF-GEISLKARKTIAMLSQDFISDGSTMLVH 588
           +IERGE F   ISL +R  IA L   FI DG+ +L H
Sbjct: 93  MIERGELFLSRISL-SRTKIASLCHAFIKDGARILTH 128



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>EI2BA_MACFA (Q4R4V8) Translation initiation factor eIF-2B alpha subunit (eIF-2B|
           GDP-GTP exchange factor)
          Length = 305

 Score = 53.9 bits (128), Expect = 3e-07
 Identities = 34/96 (35%), Positives = 53/96 (55%)
 Frame = +1

Query: 301 RSSRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSR 480
           +  +  T+  L   L  A + L   D+ S+++S+  +LF+RF++ TS LE+  +   K  
Sbjct: 35  KRDKGETIQGLRANLTSAIETLCGVDS-SVAVSSGGELFLRFISLTS-LEYSDYSKCKKI 92

Query: 481 LIERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588
           +IERGE F      +R  IA L   FI DG+T+L H
Sbjct: 93  MIERGELFLRRISLSRNKIADLCHTFIKDGATILTH 128



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>EI2BA_HUMAN (Q14232) Translation initiation factor eIF-2B alpha subunit (eIF-2B|
           GDP-GTP exchange factor)
          Length = 305

 Score = 52.0 bits (123), Expect = 1e-06
 Identities = 33/96 (34%), Positives = 52/96 (54%)
 Frame = +1

Query: 301 RSSRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSR 480
           +  +  T+  L   L  A + L   D+ S+++S+  +LF+RF++  S LE+  +   K  
Sbjct: 35  KRDKGETIQGLRANLTSAIETLCGVDS-SVAVSSGGELFLRFISLAS-LEYSDYSKCKKI 92

Query: 481 LIERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588
           +IERGE F      +R  IA L   FI DG+T+L H
Sbjct: 93  MIERGELFLRRISLSRNKIADLCHTFIKDGATILTH 128



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>EI2BA_MOUSE (Q99LC8) Translation initiation factor eIF-2B alpha subunit (eIF-2B|
           GDP-GTP exchange factor)
          Length = 305

 Score = 50.4 bits (119), Expect = 4e-06
 Identities = 33/96 (34%), Positives = 51/96 (53%)
 Frame = +1

Query: 301 RSSRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSR 480
           +  +  T+  L   L  A   L   D+ S+++S+  +LF+RF++ TS LE+  +   K  
Sbjct: 35  KRDKGETLQGLRANLTYAIKTLCGVDS-SVAVSSGGELFLRFISLTS-LEYSDYSKCKKI 92

Query: 481 LIERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588
           +IERGE F      +R  IA L   FI DG+ +L H
Sbjct: 93  MIERGELFLRRISLSRNKIANLCHTFIKDGARILTH 128



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>EI2BA_CAEEL (P34604) Putative translation initiation factor eIF-2B alpha|
           subunit (eIF-2B GDP-GTP exchange factor)
          Length = 305

 Score = 40.0 bits (92), Expect = 0.005
 Identities = 28/96 (29%), Positives = 46/96 (47%), Gaps = 2/96 (2%)
 Frame = +1

Query: 307 SRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRF--VTRTSHLEHEKFDAAKSR 480
           SRATT+ E + EL +    L+  D +S S+ +A DLF RF  +   + L+ E F      
Sbjct: 37  SRATTVAEFQNELNQVVAALEKTDYSSTSIRSAADLFTRFTSLAPAALLDQEDFSQVLDL 96

Query: 481 LIERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588
             +R   F +    +R  I+  ++ F +    +L H
Sbjct: 97  YRQRARSFIKNVRGSRAKISKCARLFFTHHMNILTH 132



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>EI2B2_METJA (Q57586) Putative translation initiation factor eIF-2B subunit 2|
           (eIF-2B GDP-GTP exchange factor)
          Length = 308

 Score = 35.4 bits (80), Expect = 0.12
 Identities = 26/87 (29%), Positives = 46/87 (52%)
 Frame = +1

Query: 328 ELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLIERGEKFG 507
           E + ++++A + L S   T++SL       +++V +  + E+ K      R+IER ++F 
Sbjct: 51  EFKNKMREAGNILISARPTAVSLPNV----VKYVLKGLNEENPK-----ERVIERADEFI 101

Query: 508 EISLKARKTIAMLSQDFISDGSTMLVH 588
             SLKA + I     + I DG T+L H
Sbjct: 102 NSSLKAIENIGKFGANRIKDGDTILTH 128



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>EI2B1_METJA (Q57896) Putative translation initiation factor eIF-2B subunit 1|
           (eIF-2B GDP-GTP exchange factor)
          Length = 329

 Score = 30.8 bits (68), Expect = 3.0
 Identities = 21/83 (25%), Positives = 41/83 (49%)
 Frame = +1

Query: 340 ELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLIERGEKFGEISL 519
           ++ KA + LK+   T+++L  A D  +     T++ E +        +++  +K  E  +
Sbjct: 72  DIYKAYEVLKNTRPTAVNLFWALDRCL-----TAYKEGKS-------ILDEAKKIHEEDI 119

Query: 520 KARKTIAMLSQDFISDGSTMLVH 588
           +  K I M+ +  I DG T+L H
Sbjct: 120 ETCKKIGMIGEKLIEDGDTILTH 142



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>KTHY_CHLPN (Q9Z8R5) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase)|
          Length = 206

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 15/60 (25%), Positives = 29/60 (48%)
 Frame = +1

Query: 388 ISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLIERGEKFGEISLKARKTIAMLSQDFISD 567
           + LS  C+LF+   +R  H++     A +   I   E+F + ++  +     L  DF++D
Sbjct: 56  LELSRCCELFLFLGSRAQHIQEVIIPALRDGYIVICERFHDSTIVYQGIAEGLGADFVAD 115



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>SEP15_HUMAN (O60613) 15 kDa selenoprotein precursor|
          Length = 162

 Score = 29.3 bits (64), Expect = 8.8
 Identities = 15/39 (38%), Positives = 21/39 (53%)
 Frame = -2

Query: 548 LSIAIVLRAFNDISPNFSPRSIRRDFAASNFSCSKCDVL 432
           L +A VL+A +     FS  + R    +SN  CS CD+L
Sbjct: 17  LLLATVLQAVSAFGAEFSSEACRELGFSSNLLCSSCDLL 55


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.317    0.129    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,732,301
Number of Sequences: 219361
Number of extensions: 731004
Number of successful extensions: 2179
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 2144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2173
length of database: 80,573,946
effective HSP length: 106
effective length of database: 57,321,680
effective search space used: 5101629520
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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