Clone Name | bart28b04 |
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Clone Library Name | barley_pub |
>EI2BA_SCHPO (Q9USP0) Translation initiation factor eIF-2B alpha subunit (eIF-2B| GDP-GTP exchange factor) Length = 341 Score = 63.9 bits (154), Expect = 3e-10 Identities = 38/94 (40%), Positives = 53/94 (56%) Frame = +1 Query: 307 SRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLI 486 S+A T+ E L+ S+ LK +ISLSA CD+F RFVTR+ H + F+ K L+ Sbjct: 50 SQAKTISEFMDILQNGSNTLKEGVQNNISLSAGCDIFQRFVTRSLH-DVGDFEQCKRHLV 108 Query: 487 ERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588 E G+ F + + R+ IA L I DGS +L H Sbjct: 109 ENGKLFIQRARACRQRIAHLGYPLIRDGSVILTH 142
>EI2BA_YEAST (P14741) Translation initiation factor eIF-2B alpha subunit (eIF-2B| GDP-GTP exchange factor) (Guanine nucleotide exchange factor subunit GCN3) (GCD complex subunit GCN3) (Transcriptional activator GCN3) Length = 305 Score = 62.0 bits (149), Expect = 1e-09 Identities = 35/90 (38%), Positives = 52/90 (57%) Frame = +1 Query: 319 TMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLIERGE 498 T E+ +K ++++L S+SL A CD+FMRFV R HL + ++ K LIE G+ Sbjct: 41 TAAEMINTIKSSTEELIKSIPNSVSLRAGCDIFMRFVLRNLHL-YGDWENCKQHLIENGQ 99 Query: 499 KFGEISLKARKTIAMLSQDFISDGSTMLVH 588 F + K+R IA + DFI+D +LVH Sbjct: 100 LFVSRAKKSRNKIAEIGVDFIADDDIILVH 129
>EI2BA_RAT (Q64270) Translation initiation factor eIF-2B alpha subunit (eIF-2B| GDP-GTP exchange factor) Length = 305 Score = 54.3 bits (129), Expect = 3e-07 Identities = 37/97 (38%), Positives = 57/97 (58%), Gaps = 1/97 (1%) Frame = +1 Query: 301 RSSRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSR 480 + + T+ L L KA + L + D+ S+++S+ +LF+RF++ TS LE+ + K Sbjct: 35 KRDKGETIQGLRAHLTKAIETLCAVDS-SVAVSSGGELFLRFISLTS-LEYSDYSKCKKI 92 Query: 481 LIERGEKF-GEISLKARKTIAMLSQDFISDGSTMLVH 588 +IERGE F ISL +R IA L FI DG+ +L H Sbjct: 93 MIERGELFLSRISL-SRTKIASLCHAFIKDGARILTH 128
>EI2BA_MACFA (Q4R4V8) Translation initiation factor eIF-2B alpha subunit (eIF-2B| GDP-GTP exchange factor) Length = 305 Score = 53.9 bits (128), Expect = 3e-07 Identities = 34/96 (35%), Positives = 53/96 (55%) Frame = +1 Query: 301 RSSRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSR 480 + + T+ L L A + L D+ S+++S+ +LF+RF++ TS LE+ + K Sbjct: 35 KRDKGETIQGLRANLTSAIETLCGVDS-SVAVSSGGELFLRFISLTS-LEYSDYSKCKKI 92 Query: 481 LIERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588 +IERGE F +R IA L FI DG+T+L H Sbjct: 93 MIERGELFLRRISLSRNKIADLCHTFIKDGATILTH 128
>EI2BA_HUMAN (Q14232) Translation initiation factor eIF-2B alpha subunit (eIF-2B| GDP-GTP exchange factor) Length = 305 Score = 52.0 bits (123), Expect = 1e-06 Identities = 33/96 (34%), Positives = 52/96 (54%) Frame = +1 Query: 301 RSSRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSR 480 + + T+ L L A + L D+ S+++S+ +LF+RF++ S LE+ + K Sbjct: 35 KRDKGETIQGLRANLTSAIETLCGVDS-SVAVSSGGELFLRFISLAS-LEYSDYSKCKKI 92 Query: 481 LIERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588 +IERGE F +R IA L FI DG+T+L H Sbjct: 93 MIERGELFLRRISLSRNKIADLCHTFIKDGATILTH 128
>EI2BA_MOUSE (Q99LC8) Translation initiation factor eIF-2B alpha subunit (eIF-2B| GDP-GTP exchange factor) Length = 305 Score = 50.4 bits (119), Expect = 4e-06 Identities = 33/96 (34%), Positives = 51/96 (53%) Frame = +1 Query: 301 RSSRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSR 480 + + T+ L L A L D+ S+++S+ +LF+RF++ TS LE+ + K Sbjct: 35 KRDKGETLQGLRANLTYAIKTLCGVDS-SVAVSSGGELFLRFISLTS-LEYSDYSKCKKI 92 Query: 481 LIERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588 +IERGE F +R IA L FI DG+ +L H Sbjct: 93 MIERGELFLRRISLSRNKIANLCHTFIKDGARILTH 128
>EI2BA_CAEEL (P34604) Putative translation initiation factor eIF-2B alpha| subunit (eIF-2B GDP-GTP exchange factor) Length = 305 Score = 40.0 bits (92), Expect = 0.005 Identities = 28/96 (29%), Positives = 46/96 (47%), Gaps = 2/96 (2%) Frame = +1 Query: 307 SRATTMMELEIELKKASDKLKSWDATSISLSAACDLFMRF--VTRTSHLEHEKFDAAKSR 480 SRATT+ E + EL + L+ D +S S+ +A DLF RF + + L+ E F Sbjct: 37 SRATTVAEFQNELNQVVAALEKTDYSSTSIRSAADLFTRFTSLAPAALLDQEDFSQVLDL 96 Query: 481 LIERGEKFGEISLKARKTIAMLSQDFISDGSTMLVH 588 +R F + +R I+ ++ F + +L H Sbjct: 97 YRQRARSFIKNVRGSRAKISKCARLFFTHHMNILTH 132
>EI2B2_METJA (Q57586) Putative translation initiation factor eIF-2B subunit 2| (eIF-2B GDP-GTP exchange factor) Length = 308 Score = 35.4 bits (80), Expect = 0.12 Identities = 26/87 (29%), Positives = 46/87 (52%) Frame = +1 Query: 328 ELEIELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLIERGEKFG 507 E + ++++A + L S T++SL +++V + + E+ K R+IER ++F Sbjct: 51 EFKNKMREAGNILISARPTAVSLPNV----VKYVLKGLNEENPK-----ERVIERADEFI 101 Query: 508 EISLKARKTIAMLSQDFISDGSTMLVH 588 SLKA + I + I DG T+L H Sbjct: 102 NSSLKAIENIGKFGANRIKDGDTILTH 128
>EI2B1_METJA (Q57896) Putative translation initiation factor eIF-2B subunit 1| (eIF-2B GDP-GTP exchange factor) Length = 329 Score = 30.8 bits (68), Expect = 3.0 Identities = 21/83 (25%), Positives = 41/83 (49%) Frame = +1 Query: 340 ELKKASDKLKSWDATSISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLIERGEKFGEISL 519 ++ KA + LK+ T+++L A D + T++ E + +++ +K E + Sbjct: 72 DIYKAYEVLKNTRPTAVNLFWALDRCL-----TAYKEGKS-------ILDEAKKIHEEDI 119 Query: 520 KARKTIAMLSQDFISDGSTMLVH 588 + K I M+ + I DG T+L H Sbjct: 120 ETCKKIGMIGEKLIEDGDTILTH 142
>KTHY_CHLPN (Q9Z8R5) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase)| Length = 206 Score = 29.3 bits (64), Expect = 8.8 Identities = 15/60 (25%), Positives = 29/60 (48%) Frame = +1 Query: 388 ISLSAACDLFMRFVTRTSHLEHEKFDAAKSRLIERGEKFGEISLKARKTIAMLSQDFISD 567 + LS C+LF+ +R H++ A + I E+F + ++ + L DF++D Sbjct: 56 LELSRCCELFLFLGSRAQHIQEVIIPALRDGYIVICERFHDSTIVYQGIAEGLGADFVAD 115
>SEP15_HUMAN (O60613) 15 kDa selenoprotein precursor| Length = 162 Score = 29.3 bits (64), Expect = 8.8 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = -2 Query: 548 LSIAIVLRAFNDISPNFSPRSIRRDFAASNFSCSKCDVL 432 L +A VL+A + FS + R +SN CS CD+L Sbjct: 17 LLLATVLQAVSAFGAEFSSEACRELGFSSNLLCSSCDLL 55 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.317 0.129 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 52,732,301 Number of Sequences: 219361 Number of extensions: 731004 Number of successful extensions: 2179 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 2144 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 2173 length of database: 80,573,946 effective HSP length: 106 effective length of database: 57,321,680 effective search space used: 5101629520 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)