ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart27g05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1IMDH_METJA (Q59011) Inosine-5'-monophosphate dehydrogenase (EC 1... 34 0.24
2CWC22_MAGGR (Q52B63) Pre-mRNA-splicing factor CWC22 32 1.6
3CYSJ_ECO57 (Q8X7U1) Sulfite reductase [NADPH] flavoprotein alpha... 30 3.5
4AROK_PYRKO (Q5JFT2) Shikimate kinase (EC 2.7.1.71) (SK) 30 4.5
5GCST_NOCFA (Q5YZ49) Aminomethyltransferase (EC 2.1.2.10) (Glycin... 30 4.5
6GCST_GEOKA (Q5KX76) Aminomethyltransferase (EC 2.1.2.10) (Glycin... 30 4.5
7CYSJ_SALTY (P38039) Sulfite reductase [NADPH] flavoprotein alpha... 30 4.5
8CYSJ_SALTI (Q8Z458) Sulfite reductase [NADPH] flavoprotein alpha... 30 4.5
9MUTS_TREDE (P61672) DNA mismatch repair protein mutS 30 5.9
10THIC_STRCO (Q9X9U0) Thiamine biosynthesis protein thiC 29 7.8
11RPOB_LEIXX (Q6ACX5) DNA-directed RNA polymerase beta chain (EC 2... 29 7.8

>IMDH_METJA (Q59011) Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) (IMP|
           dehydrogenase) (IMPDH) (IMPD)
          Length = 496

 Score = 34.3 bits (77), Expect = 0.24
 Identities = 21/72 (29%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
 Frame = -1

Query: 446 ESPGRQLVAVAVVDEPLLRQTQLLAADGEEGEAVGVHE-YDLAGRAAGVGLDAEDEVAVA 270
           E    Q+ AV   DE +++    ++ D   GEA+ V E Y ++G      +D ED++   
Sbjct: 80  EEQVHQVQAVKKADEVVIKDVITVSPDDTVGEAINVMETYSISGLPV---VDNEDKLVGI 136

Query: 269 VEHREPLAVEEQ 234
           + HR+  A+E++
Sbjct: 137 ITHRDVKAIEDK 148



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>CWC22_MAGGR (Q52B63) Pre-mRNA-splicing factor CWC22|
          Length = 907

 Score = 31.6 bits (70), Expect = 1.6
 Identities = 17/40 (42%), Positives = 23/40 (57%)
 Frame = -1

Query: 548 PAARRIPSRTVQRSRRRQATEGLCREQTHVPPRRESPGRQ 429
           P   R  SR+V RSR R  +  + R  T  PPRR++P +Q
Sbjct: 721 PIRGRSYSRSVSRSRSRSYSRSVSRSPT--PPRRQAPAQQ 758



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>CYSJ_ECO57 (Q8X7U1) Sulfite reductase [NADPH] flavoprotein alpha-component (EC|
           1.8.1.2) (SIR-FP)
          Length = 598

 Score = 30.4 bits (67), Expect = 3.5
 Identities = 28/80 (35%), Positives = 39/80 (48%), Gaps = 15/80 (18%)
 Frame = -1

Query: 449 RESPGRQLVAVAVVD--EPLLRQTQLLAADGEEGE-----AVGVHEYDLAGRAAGVG--- 300
           R SP  QL A A+++   PL  +   +A+  EE E      VGV  YD+ GRA   G   
Sbjct: 364 RFSPA-QLDAEALINLLRPLTPRLYSIASSQEEVENEVHVTVGVVRYDVEGRARAGGASS 422

Query: 299 -----LDAEDEVAVAVEHRE 255
                ++ E EV V +EH +
Sbjct: 423 FLADRVEEEGEVRVFIEHND 442



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>AROK_PYRKO (Q5JFT2) Shikimate kinase (EC 2.7.1.71) (SK)|
          Length = 271

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 23/70 (32%), Positives = 35/70 (50%), Gaps = 3/70 (4%)
 Frame = +3

Query: 201 EAVSLTV---WRRSLLFNGKGFTVFDSNGNLVFRVETYARGSPREVVLMDADGLALLTVR 371
           EAV L +   WR++ L NG  ++ +  +    FR+    RG+   VV +   G A+  V 
Sbjct: 194 EAVRLALEGEWRKAALINGLVYSTYLGHPIEPFRI-ALERGA---VVGLSGKGPAVFAVT 249

Query: 372 RKKLSLAEEW 401
            +   LAEEW
Sbjct: 250 NEPEELAEEW 259



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>GCST_NOCFA (Q5YZ49) Aminomethyltransferase (EC 2.1.2.10) (Glycine cleavage|
           system T protein)
          Length = 366

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 30/83 (36%), Positives = 38/83 (45%), Gaps = 11/83 (13%)
 Frame = -1

Query: 440 PGRQLVAVAVVDEPLLRQTQLLAADGEE-GEA----------VGVHEYDLAGRAAGVGLD 294
           P R L+ +  +D  +LRQ Q +  DGE  GE           VG+    LA    G GL+
Sbjct: 281 PRRILMGLKAIDRGVLRQGQPVLRDGEPVGETTSGTFSPTLKVGI---ALALLDTGAGLE 337

Query: 293 AEDEVAVAVEHREPLAVEEQRPP 225
              EVAV V  R  L  E  +PP
Sbjct: 338 PGAEVAVDVRGRR-LRCEVVKPP 359



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>GCST_GEOKA (Q5KX76) Aminomethyltransferase (EC 2.1.2.10) (Glycine cleavage|
           system T protein)
          Length = 364

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = -1

Query: 452 RRESPGRQLVAVAVVDEPLLRQTQLLAADGEE 357
           + E P R+LV + ++D  + R   L+ ADGEE
Sbjct: 277 KEEGPPRRLVGIEMIDRGIPRHGYLVFADGEE 308



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>CYSJ_SALTY (P38039) Sulfite reductase [NADPH] flavoprotein alpha-component (EC|
           1.8.1.2) (SIR-FP)
          Length = 598

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 28/80 (35%), Positives = 39/80 (48%), Gaps = 15/80 (18%)
 Frame = -1

Query: 449 RESPGRQLVAVAVVD--EPLLRQTQLLAADGEEGEA-----VGVHEYDLAGRAAGVG--- 300
           R SP  QL A A++D   PL  +   +A+   E E+     VGV  YD+ GRA   G   
Sbjct: 364 RFSPA-QLDAQALIDLLRPLTPRLYSIASAQAEVESEVHITVGVVRYDIEGRARAGGASS 422

Query: 299 -----LDAEDEVAVAVEHRE 255
                ++ E EV V +EH +
Sbjct: 423 FLADRVEEEGEVRVFIEHND 442



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>CYSJ_SALTI (Q8Z458) Sulfite reductase [NADPH] flavoprotein alpha-component (EC|
           1.8.1.2) (SIR-FP)
          Length = 598

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 28/80 (35%), Positives = 39/80 (48%), Gaps = 15/80 (18%)
 Frame = -1

Query: 449 RESPGRQLVAVAVVD--EPLLRQTQLLAADGEEGEA-----VGVHEYDLAGRAAGVG--- 300
           R SP  QL A A++D   PL  +   +A+   E E+     VGV  YD+ GRA   G   
Sbjct: 364 RFSPA-QLDAQALIDLLRPLTPRLYSIASAQAEVESEVHITVGVVRYDIEGRARAGGASS 422

Query: 299 -----LDAEDEVAVAVEHRE 255
                ++ E EV V +EH +
Sbjct: 423 FLADRVEEEGEVRVFIEHND 442



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>MUTS_TREDE (P61672) DNA mismatch repair protein mutS|
          Length = 891

 Score = 29.6 bits (65), Expect = 5.9
 Identities = 15/56 (26%), Positives = 26/56 (46%)
 Frame = -1

Query: 365 GEEGEAVGVHEYDLAGRAAGVGLDAEDEVAVAVEHREPLAVEEQRPPPHGERHRLP 198
           G  G + G+H   LAG    V   AE+ + +  + ++   ++E RP   G   + P
Sbjct: 788 GAAGNSYGIHVAGLAGIPQSVLTRAENLLYMRSQFQKERTIQEARPSAQGSEEKTP 843



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>THIC_STRCO (Q9X9U0) Thiamine biosynthesis protein thiC|
          Length = 612

 Score = 29.3 bits (64), Expect = 7.8
 Identities = 22/72 (30%), Positives = 32/72 (44%)
 Frame = +3

Query: 225 RRSLLFNGKGFTVFDSNGNLVFRVETYARGSPREVVLMDADGLALLTVRRKKLSLAEEWL 404
           R+  L NG+  T++D++G             P    L+D D      VRR    L E W+
Sbjct: 55  RQVHLTNGQSVTLYDTSG-------------PYTDPLVDTD------VRRGLAPLRENWI 95

Query: 405 VYDGDGDELAPR 440
           +  GD +E A R
Sbjct: 96  IARGDTEEYAGR 107



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>RPOB_LEIXX (Q6ACX5) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP|
           beta subunit) (Transcriptase beta chain) (RNA polymerase
           beta subunit)
          Length = 1165

 Score = 29.3 bits (64), Expect = 7.8
 Identities = 17/62 (27%), Positives = 28/62 (45%)
 Frame = -1

Query: 395 LRQTQLLAADGEEGEAVGVHEYDLAGRAAGVGLDAEDEVAVAVEHREPLAVEEQRPPPHG 216
           +R T L    GEEG  +GV  +D          D +DE+   V  R  + + ++R    G
Sbjct: 822 VRDTSLKVPHGEEGTIIGVKVFD--------AQDGDDELGSGVNQRVVVYIAQKRKITAG 873

Query: 215 ER 210
           ++
Sbjct: 874 DK 875


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,421,643
Number of Sequences: 219361
Number of extensions: 1352004
Number of successful extensions: 4401
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4400
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4488201198
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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