ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | bart26g07 |
|---|---|
| Clone Library Name | barley_pub |
>SC5D_TOBAC (Q9ZT29) Delta-7-sterol-C5(6)-desaturase (EC 1.3.3.-) (Delta-7-C-5| sterol desaturase) (Delta7-sterol-C5-desaturase) Length = 271 Score = 212 bits (539), Expect = 6e-55 Identities = 97/152 (63%), Positives = 114/152 (75%) Frame = +2 Query: 89 EDYWGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCF 268 EDY F EET +YN + L +P WW LPH L+ WLRN IGG LLYF +GFLWCF Sbjct: 2 EDYLKQFVEETSFYNRLVLGTFMPES--WWGPLPHMLQGWLRNYIGGVLLYFISGFLWCF 59 Query: 269 VIYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCFFHISE 448 IY+ KRN YIPKDA+P+ +AM QI VA KAMP+YCALPS+SE+MIE+GWT+CF IS+ Sbjct: 60 YIYHLKRNVYIPKDAIPSNKAMLLQISVAMKAMPWYCALPSLSEYMIENGWTKCFARISD 119 Query: 449 VGWPMYFVYVALYLTFVEFGIYWMHRELHDIK 544 VGW Y +Y A+YL VEFGIYWMH ELHDIK Sbjct: 120 VGWLSYVIYAAIYLVIVEFGIYWMHMELHDIK 151
>SC5D1_ARATH (Q39208) Delta-7-sterol-C5(6)-desaturase 1 (EC 1.3.3.-)| (Delta-7-C-5 sterol desaturase 1) (Delta7-sterol-C5-desaturase 1) (STEROL1 protein) (Dwarf 7 protein) Length = 281 Score = 204 bits (518), Expect = 2e-52 Identities = 92/146 (63%), Positives = 112/146 (76%) Frame = +2 Query: 107 FREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFVIYYWK 286 F +ET +YN I LS ++P W LPH L++WLRN + G LLYF +GFLWCF IYY K Sbjct: 11 FVDETSFYNRIVLSHLLPAN--LWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYYLK 68 Query: 287 RNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCFFHISEVGWPMY 466 N Y+PKDA+PT++AM+ Q+ VA KAMP+Y LP+VSE MIE GWT+CF I E GW +Y Sbjct: 69 INVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASIGEFGWILY 128 Query: 467 FVYVALYLTFVEFGIYWMHRELHDIK 544 FVY+A+YL FVEFGIYWMHRELHDIK Sbjct: 129 FVYIAIYLVFVEFGIYWMHRELHDIK 154
>SC5D2_ARATH (Q9M883) Putative delta-7-sterol-C5(6)-desaturase 2 (EC 1.3.3.-)| (Delta-7-C-5 sterol desaturase 2) (Delta7-sterol-C5-desaturase 2) (Homolog of DWF7 protein) Length = 279 Score = 177 bits (448), Expect = 2e-44 Identities = 81/151 (53%), Positives = 106/151 (70%) Frame = +2 Query: 92 DYWGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV 271 DY ET +YN + LS ++P W LPH L++WLRN + G +LYF +GFLWCF Sbjct: 7 DYNDQIVNETSFYNRMVLSHLLPVN--LWEPLPHFLQTWLRNYLAGNILYFISGFLWCFY 64 Query: 272 IYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCFFHISEV 451 IYY K N Y+PK+++PT +AM QI VA KAMP+Y LP+VSE+MIE GWT+C+ + Sbjct: 65 IYYLKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHF 124 Query: 452 GWPMYFVYVALYLTFVEFGIYWMHRELHDIK 544 W + F+Y+ALYL VEF IYW+H+ELHDIK Sbjct: 125 NWFLCFLYIALYLVLVEFMIYWVHKELHDIK 155
>SC5D_MOUSE (O88822) Lathosterol oxidase (EC 1.14.21.6) (Lathosterol| 5-desaturase) (Delta-7-sterol 5-desaturase) (C-5 sterol desaturase) (Sterol-C5-desaturase) Length = 299 Score = 42.4 bits (98), Expect = 9e-04 Identities = 30/108 (27%), Positives = 55/108 (50%), Gaps = 3/108 (2%) Frame = +2 Query: 221 IGGYLLYFATGFLWCFVIYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSE 400 +G Y+LYF L YY+ + + K + ++I+ K++P + ++P+VS Sbjct: 40 LGAYILYFFCATL----SYYFVYDHSLMKHPQFLKNQVSREIVFTVKSLP-WISIPTVSL 94 Query: 401 HMIE-SGWTRCFFHISEV--GWPMYFVYVALYLTFVEFGIYWMHRELH 535 ++E G+++ + I + GW V V +L F + IY +HR LH Sbjct: 95 FLLELRGYSKLYDDIGDFPNGWIHLMVSVVSFLFFTDMLIYRIHRGLH 142
>ERG32_SCHPO (O13666) Probable C-5 sterol desaturase 2 (EC 1.3.3.-)| (Sterol-C5-desaturase 2) (Ergosterol delta 5,6 desaturase 2) Length = 329 Score = 39.7 bits (91), Expect = 0.006 Identities = 29/105 (27%), Positives = 50/105 (47%) Frame = +2 Query: 221 IGGYLLYFATGFLWCFVIYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSE 400 I G L YF + + + +Y+ + A + E + +++VA K +P L + Sbjct: 73 IMGTLSYFLSAS-FAYYVYFDREEARRHPKFLKNQEHL--ELMVALKNLPGMAILTAPWF 129 Query: 401 HMIESGWTRCFFHISEVGWPMYFVYVALYLTFVEFGIYWMHRELH 535 G+ + + E G+ F +AL+L F +F IYW+HR LH Sbjct: 130 LAEIRGYGYVYDKLDEYGYFYLFFSIALFLLFSDFLIYWIHRALH 174
>ERG31_SCHPO (O94457) Probable C-5 sterol desaturase 1 (EC 1.3.3.-)| (Sterol-C5-desaturase 1) (Ergosterol delta 5,6 desaturase 1) Length = 300 Score = 37.7 bits (86), Expect = 0.021 Identities = 15/40 (37%), Positives = 25/40 (62%) Frame = +2 Query: 416 GWTRCFFHISEVGWPMYFVYVALYLTFVEFGIYWMHRELH 535 G++ + +IS+ G + + L++ F +FGIYW HR LH Sbjct: 102 GYSYLYDNISDYGLKYFLCSLPLFVMFSDFGIYWAHRFLH 141
>SC5D_HUMAN (O75845) Lathosterol oxidase (EC 1.14.21.6) (Lathosterol| 5-desaturase) (Delta-7-sterol 5-desaturase) (C-5 sterol desaturase) (Sterol-C5-desaturase) Length = 299 Score = 37.4 bits (85), Expect = 0.027 Identities = 27/108 (25%), Positives = 56/108 (51%), Gaps = 3/108 (2%) Frame = +2 Query: 221 IGGYLLYFATGFLWCFVIYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSE 400 +G Y+LYF L YY+ + + K ++++I +A+P+ L +V+ Sbjct: 40 VGAYILYFFCATL----SYYFVFDHALMKHPQFLKNQVRREIKFTVQALPWISIL-TVAL 94 Query: 401 HMIE-SGWTRCFFHISEVGWPMYFVYVAL--YLTFVEFGIYWMHRELH 535 ++E G+++ + E + ++ + V++ +L F + IYW+HR LH Sbjct: 95 FLLEIRGYSKLHDDLGEFPYGLFELVVSIISFLFFTDMFIYWIHRGLH 142
>ERG3_NEUCR (Q7SBB6) Probable C-5 sterol desaturase (EC 1.3.3.-)| (Sterol-C5-desaturase) (Ergosterol delta 5,6 desaturase) Length = 344 Score = 33.9 bits (76), Expect = 0.30 Identities = 12/23 (52%), Positives = 16/23 (69%) Frame = +2 Query: 467 FVYVALYLTFVEFGIYWMHRELH 535 F L++ F +FGIYW+HR LH Sbjct: 163 FFQFPLFIMFTDFGIYWIHRGLH 185
>ERG3_CANAL (O93875) C-5 sterol desaturase (EC 1.3.3.-) (Sterol-C5-desaturase)| (Ergosterol delta 5,6 desaturase) Length = 386 Score = 32.7 bits (73), Expect = 0.67 Identities = 29/108 (26%), Positives = 54/108 (50%), Gaps = 3/108 (2%) Frame = +2 Query: 221 IGGYLLYFATGFLWCFVIYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSE 400 I G+LLYF +L +V + K+ P+ M +I A+ A+P L ++ Sbjct: 128 IFGWLLYFIVAYL-SYVFVFDKKIFNHPRYLK---NQMSLEIKRATTAIPVMVLL-TIPF 182 Query: 401 HMIE-SGWTRCFFHISEV--GWPMYFVYVALYLTFVEFGIYWMHRELH 535 ++E +G++ + I+E G+ + ++ F + GIY++HR LH Sbjct: 183 FLLELNGYSFLYLDINECTGGYKAILWQIPKFILFTDCGIYFLHRWLH 230
>SGLT_VIBPA (P96169) Sodium/glucose cotransporter (Na(+)/glucose symporter)| Length = 543 Score = 32.7 bits (73), Expect = 0.67 Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 5/60 (8%) Frame = +2 Query: 251 GFLWCFVIYYWKRNAYIPKD--AVPTVEAMKKQIIVAS---KAMPFYCALPSVSEHMIES 415 G LW +YYW N YI + A +V +K I+ A+ +PF LP ++ ++I S Sbjct: 254 GGLWVANLYYWGFNQYIIQRTLAAKSVSEAQKGIVFAAFLKLIVPFLVVLPGIAAYVITS 313
>BSU1_SCHPO (P33532) Vitamin B6 transporter bsu1 (Vitamin B6 uptake protein 1)| (Changed amiloride resistance protein 1) (Amiloride transporter car1) Length = 526 Score = 30.8 bits (68), Expect = 2.6 Identities = 14/60 (23%), Positives = 28/60 (46%) Frame = +2 Query: 101 GLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFVIYY 280 G+ R E + + +F++ + P G + HP W+ +G ++ A G W ++ Y Sbjct: 379 GVARPEARFTSALFITLLFPAGMFLFAFTCHPPFPWMSPIVGNSMVTVANGHNWMCILNY 438
>ERG3_CANDU (Q8NJ57) C-5 sterol desaturase (EC 1.3.3.-) (Sterol-C5-desaturase)| (Ergosterol delta 5,6 desaturase) Length = 386 Score = 30.8 bits (68), Expect = 2.6 Identities = 28/106 (26%), Positives = 52/106 (49%), Gaps = 3/106 (2%) Frame = +2 Query: 227 GYLLYFATGFLWCFVIYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHM 406 G+LLYF +L +V + K+ P+ M +I A+ A+P L ++ + Sbjct: 130 GWLLYFIVAYL-SYVFVFDKKIFNHPRYLK---NQMSLEIKRATSAIPVMVLL-TIPFFL 184 Query: 407 IE-SGWTRCFFHISEV--GWPMYFVYVALYLTFVEFGIYWMHRELH 535 +E G++ + I+E G+ + ++ F + GIY++HR LH Sbjct: 185 LELHGYSFLYEEINESTGGYKAILWQIPKFILFTDCGIYFLHRWLH 230
>ERG3_ASHGO (Q754B9) C-5 sterol desaturase (EC 1.3.3.-) (Sterol-C5-desaturase)| (Ergosterol delta 5,6 desaturase) Length = 351 Score = 30.4 bits (67), Expect = 3.3 Identities = 28/121 (23%), Positives = 54/121 (44%), Gaps = 7/121 (5%) Frame = +2 Query: 194 PLRSWLRNCIGGYLLYFATGFLWCFVIYYWKRNAYIPKDAVPTVEAMKKQIIVASK---- 361 P S LR + +L+ G+L ++ + K +K Q+ + K Sbjct: 79 PRYSLLRQSLSLFLVTTVFGWLLYLIVASFSYVFVFDKSVFNHPRYLKNQMSMEIKQGLG 138 Query: 362 AMPFYCALPSVSEHMIE-SGWTRCFF--HISEVGWPMYFVYVALYLTFVEFGIYWMHREL 532 A+P Y A+ +V ++E G++ + ++ G+ + ++ F +FGIY +HR L Sbjct: 139 AIP-YMAVMTVPWFLLELHGYSHLYMGLELNVRGYVRLALKALFFILFTDFGIYLLHRWL 197 Query: 533 H 535 H Sbjct: 198 H 198
>OR30A_DROME (Q9VLE5) Putative odorant receptor 30a| Length = 377 Score = 30.0 bits (66), Expect = 4.4 Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 4/60 (6%) Frame = +2 Query: 344 IIVASKAMPFYCALPSVSEHMIESGWTRCFFHISEV----GWPMYFVYVALYLTFVEFGI 511 I + + +P+ LP++ E+ S + FF I + G MY Y + +TF F I Sbjct: 145 IFGSERVLPYGMYLPTIDEYKYASPYYEIFFVIQAIMAPMGCCMYIPYTNMVVTFTLFAI 204
>WBP11_BRARE (Q6P0D5) WW domain-binding protein 11 (WBP-11)| Length = 640 Score = 29.6 bits (65), Expect = 5.7 Identities = 13/21 (61%), Positives = 14/21 (66%) Frame = -2 Query: 210 SQERSGCGSARHQPPPPGTTA 148 S E + GSA PPPPGTTA Sbjct: 512 SNEGAPSGSALQMPPPPGTTA 532
>NUOH_MYCTU (P65561) NADH-quinone oxidoreductase chain H (EC 1.6.99.5) (NADH| dehydrogenase I, chain H) (NDH-1, chain H) Length = 410 Score = 29.6 bits (65), Expect = 5.7 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = +2 Query: 170 GWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV-IYYWKRNAYIPK 307 G W A P PL W G + L + T +W F+ IY+W R A +P+ Sbjct: 265 GGWHA-PWPLNMWASANTGWWPLIWFTAKVWGFLFIYFWLR-ATLPR 309
>NUOH_MYCBO (P65562) NADH-quinone oxidoreductase chain H (EC 1.6.99.5) (NADH| dehydrogenase I, chain H) (NDH-1, chain H) Length = 410 Score = 29.6 bits (65), Expect = 5.7 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = +2 Query: 170 GWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV-IYYWKRNAYIPK 307 G W A P PL W G + L + T +W F+ IY+W R A +P+ Sbjct: 265 GGWHA-PWPLNMWASANTGWWPLIWFTAKVWGFLFIYFWLR-ATLPR 309
>PRP28_CRYNE (Q5KNF8) Pre-mRNA splicing ATP-dependent RNA helicase PRP28 (EC| 3.6.1.-) Length = 738 Score = 29.3 bits (64), Expect = 7.5 Identities = 16/47 (34%), Positives = 22/47 (46%) Frame = +2 Query: 98 WGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLL 238 W +FRE+ S GGG +PHPLR+W + I +L Sbjct: 294 WRIFRED--------FSIAARGGG-----IPHPLRNWRESAIPSQIL 327
>GLB3_TYLHE (P13578) Extracellular globin IIB (Erythrocruorin)| Length = 148 Score = 28.9 bits (63), Expect = 9.7 Identities = 11/23 (47%), Positives = 13/23 (56%) Frame = +2 Query: 383 LPSVSEHMIESGWTRCFFHISEV 451 LP VS H W RCF I++V Sbjct: 120 LPQVSSHFNSGAWDRCFTRIADV 142
>NU5M_POLOR (Q95918) NADH-ubiquinone oxidoreductase chain 5 (EC 1.6.5.3) (NADH| dehydrogenase subunit 5) Length = 613 Score = 28.9 bits (63), Expect = 9.7 Identities = 12/28 (42%), Positives = 21/28 (75%), Gaps = 2/28 (7%) Frame = +2 Query: 455 WPMYFVYVALYLTF--VEFGIYWMHREL 532 + M F+ +ALY+T+ +EF IY+MH ++ Sbjct: 90 YSMIFMTIALYVTWSILEFAIYYMHTDI 117
>ATG13_EMENI (Q5BBK4) Autophagy-related protein 13| Length = 974 Score = 28.9 bits (63), Expect = 9.7 Identities = 13/39 (33%), Positives = 21/39 (53%) Frame = -2 Query: 201 RSGCGSARHQPPPPGTTALRKISLYQSVSSRNRPQ*SSP 85 R+ +RH PPP TT+ RK + ++ + P +SP Sbjct: 470 RTSLSDSRHMPPPSVTTSSRKPPSFGPDNANSSPNSASP 508 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 59,918,345 Number of Sequences: 219361 Number of extensions: 1157698 Number of successful extensions: 3401 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 3310 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3396 length of database: 80,573,946 effective HSP length: 105 effective length of database: 57,541,041 effective search space used: 4315578075 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
