ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart19f05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1TRMB_ARATH (Q8GXB7) Probable tRNA (guanine-N(7)-)-methyltransfer... 125 7e-29
2TRMB_HUMAN (Q9UBP6) tRNA (guanine-N(7)-)-methyltransferase (EC 2... 89 9e-18
3TRMB_MOUSE (Q9Z120) tRNA (guanine-N(7)-)-methyltransferase (EC 2... 89 1e-17
4TRM8_YEAST (Q12009) tRNA (guanine-N(7)-)-methyltransferase (EC 2... 84 3e-16
5TRMB_DROME (O77263) Probable tRNA (guanine-N(7)-)-methyltransfer... 80 4e-15
6TRMB_CAEEL (Q23126) Probable tRNA (guanine-N(7)-)-methyltransfer... 78 2e-14
7TRMB_SCHPO (Q96WV1) Probable tRNA (guanine-N(7)-)-methyltransfer... 74 3e-13
8GSP2_YEAST (P32836) GTP-binding nuclear protein GSP2/CNR2 30 6.8
9Y093_MYCTU (Q10890) Hypothetical protein Rv0093c/MT0102 30 6.8
10FLIP1_RAT (Q8K4T4) Filamin-A-interacting protein 1 (FILIP) 29 8.9
11PUR1_SHIFL (P0AG17) Amidophosphoribosyltransferase (EC 2.4.2.14)... 29 8.9
12PUR1_ECOLI (P0AG16) Amidophosphoribosyltransferase (EC 2.4.2.14)... 29 8.9
13CDX2_MOUSE (P43241) Homeobox protein CDX-2 (Caudal-type homeobox... 29 8.9

>TRMB_ARATH (Q8GXB7) Probable tRNA (guanine-N(7)-)-methyltransferase (EC|
           2.1.1.33) (tRNA(m7G46)-methyltransferase)
          Length = 251

 Score =  125 bits (315), Expect = 7e-29
 Identities = 69/121 (57%), Positives = 78/121 (64%), Gaps = 1/121 (0%)
 Frame = +2

Query: 230 LPRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYPRYFPXXXXXXXXXXXXXXXXXPR 409
           LPRKRFYRARAHSNPLSDSHFPIPISP  VD S H+P++                    +
Sbjct: 16  LPRKRFYRARAHSNPLSDSHFPIPISPAHVDYSLHFPKF-----------VEADNKFIKK 64

Query: 410 IRFADXXXXXXXXXXXXSPLFPDKLMIGMELRGKVTEYVKERVLAL-RASNPGQYDNISV 586
           + FAD            + LFPD LMIGMELR KVTEYVKER+LAL R S+ GQY+NISV
Sbjct: 65  VEFADIGCGFGGLLISLATLFPDTLMIGMELRDKVTEYVKERILALRRTSSEGQYENISV 124

Query: 587 V 589
           V
Sbjct: 125 V 125



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>TRMB_HUMAN (Q9UBP6) tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33)|
           (tRNA(m7G46)-methyltransferase) (Methyltransferase-like
           protein 1)
          Length = 276

 Score = 89.0 bits (219), Expect = 9e-18
 Identities = 44/120 (36%), Positives = 68/120 (56%), Gaps = 3/120 (2%)
 Frame = +2

Query: 233 PRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYPRYFPXXXXXXXXXX---XXXXXXX 403
           P+KR+YR RAHSNP++D     P+ P+++D S+ YP +F                     
Sbjct: 16  PQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQ 75

Query: 404 PRIRFADXXXXXXXXXXXXSPLFPDKLMIGMELRGKVTEYVKERVLALRASNPGQYDNIS 583
            ++ FAD            SPLFPD L++G+E+R KV++YV++R+ ALRA+  G + NI+
Sbjct: 76  AQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPAGGFQNIA 135



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>TRMB_MOUSE (Q9Z120) tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33)|
           (tRNA(m7G46)-methyltransferase) (Methyltransferase-like
           protein 1)
          Length = 268

 Score = 88.6 bits (218), Expect = 1e-17
 Identities = 44/120 (36%), Positives = 68/120 (56%), Gaps = 3/120 (2%)
 Frame = +2

Query: 233 PRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYPRYFPXXXXXXXXXX---XXXXXXX 403
           P+KR+YR RAHSNP++D     P+ P+++D S+ YP +F                     
Sbjct: 10  PQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLIQNKSHDDPKDEKEKHSG 69

Query: 404 PRIRFADXXXXXXXXXXXXSPLFPDKLMIGMELRGKVTEYVKERVLALRASNPGQYDNIS 583
            ++ FAD            SPLFPD L++G+E+R KV++YV++R+ ALRA+  G + NI+
Sbjct: 70  AQVEFADIGCGYGGLLVALSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPGGGFQNIA 129



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>TRM8_YEAST (Q12009) tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33)|
           (tRNA(m7G46)-methyltransferase)
          Length = 286

 Score = 84.0 bits (206), Expect = 3e-16
 Identities = 48/123 (39%), Positives = 67/123 (54%), Gaps = 3/123 (2%)
 Frame = +2

Query: 230 LPRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYPRYFPXXXXXXXXXXXXXXXXXPR 409
           LP+KR+YR RAHSNP SD     P+SP D+D S+ YP Y                    +
Sbjct: 47  LPKKRYYRQRAHSNPFSDHQLEYPVSPQDMDWSKLYPYY----------KNAENGQMTKK 96

Query: 410 IRFADXXXXXXXXXXXXSPLFPDKLMIGMELRGKVTEYVKERVLALR---ASNPGQYDNI 580
           +  AD            SP FP+ L++GME+R +VT YV++R++ALR   AS  G + NI
Sbjct: 97  VTIADIGCGFGGLMIDLSPAFPEDLILGMEIRVQVTNYVEDRIIALRNNTASKHG-FQNI 155

Query: 581 SVV 589
           +V+
Sbjct: 156 NVL 158



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>TRMB_DROME (O77263) Probable tRNA (guanine-N(7)-)-methyltransferase (EC|
           2.1.1.33) (tRNA(m7G46)-methyltransferase)
          Length = 256

 Score = 80.1 bits (196), Expect = 4e-15
 Identities = 45/120 (37%), Positives = 62/120 (51%), Gaps = 2/120 (1%)
 Frame = +2

Query: 230 LPRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYPRYFPXXXXXXXXXXXXXXXXXPR 409
           LP+KR+YR RAHSNP++D  F  P  P+DVD    YP                      +
Sbjct: 29  LPQKRYYRQRAHSNPIADHSFDYPARPEDVDWRSMYP----------------GIQQGQQ 72

Query: 410 IRFADXXXXXXXXXXXXSPLFPDKLMIGMELRGKVTEYVKERVLAL--RASNPGQYDNIS 583
           + FAD              +FP+KL IGME+R KV++YV +R+ AL  R ++ G Y NI+
Sbjct: 73  VSFADIGCGYGGFLVTLGEMFPEKLSIGMEIRVKVSDYVVDRIAALRRRCADTGAYQNIA 132



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>TRMB_CAEEL (Q23126) Probable tRNA (guanine-N(7)-)-methyltransferase (EC|
           2.1.1.33) (tRNA(m7G46)-methyltransferase)
          Length = 256

 Score = 77.8 bits (190), Expect = 2e-14
 Identities = 44/122 (36%), Positives = 65/122 (53%), Gaps = 2/122 (1%)
 Frame = +2

Query: 230 LPRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYPRYFPXXXXXXXXXXXXXXXXXPR 409
           LP+K+ YR RAHSNP SD     P++P+ +D +++Y  Y                    +
Sbjct: 24  LPQKKHYRQRAHSNPHSDHDIEYPLTPNHMDWTKYYGDY----------------TKGRQ 67

Query: 410 IRFADXXXXXXXXXXXXSPLFPDKLMIGMELRGKVTEYVKERVLALRA--SNPGQYDNIS 583
           + FAD            SP +PD LMIGME+R KV++YV E++ ALR   +  G Y N++
Sbjct: 68  VDFADIGCGYGGLLMRLSPKYPDNLMIGMEIRVKVSDYVNEKIQALRKHHAEAGHYRNVA 127

Query: 584 VV 589
           V+
Sbjct: 128 VL 129



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>TRMB_SCHPO (Q96WV1) Probable tRNA (guanine-N(7)-)-methyltransferase (EC|
           2.1.1.33) (tRNA(m7G46)-methyltransferase)
          Length = 273

 Score = 73.9 bits (180), Expect = 3e-13
 Identities = 43/127 (33%), Positives = 62/127 (48%), Gaps = 6/127 (4%)
 Frame = +2

Query: 227 KLPRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYPRYFPXXXXXXXXXXXXXXXXXP 406
           +LP KR +R RAH+N LSD     P SP ++D S +YP +                    
Sbjct: 35  RLPMKRLFRQRAHANVLSDHELEYPRSPSEMDWSPYYPDF--------------DVESNK 80

Query: 407 RIRFADXXXXXXXXXXXXSPLFPDKLMIGMELRGKVTEYVKERVLALR------ASNPGQ 568
           ++   D             P FPD L++GME+R +V++Y+KE++ ALR         PG 
Sbjct: 81  KVEIVDIGCGYGGLTVALGPQFPDTLVLGMEIRMQVSDYLKEKIQALRYRADHEEPVPGG 140

Query: 569 YDNISVV 589
           Y NISV+
Sbjct: 141 YKNISVL 147



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>GSP2_YEAST (P32836) GTP-binding nuclear protein GSP2/CNR2|
          Length = 220

 Score = 29.6 bits (65), Expect = 6.8
 Identities = 14/54 (25%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
 Frame = -2

Query: 562 WIGCS*SQNPLFDILSHLA---PQLHADHELVGEERREPDEQPTKPAPDVSEAD 410
           W+    + NP  + ++  A   P++  D +L+ + ++E D+    P PD  +AD
Sbjct: 166 WLARKLAGNPQLEFVASPALAPPEVQVDEQLMHQYQQEMDQATALPLPDEDDAD 219



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>Y093_MYCTU (Q10890) Hypothetical protein Rv0093c/MT0102|
          Length = 282

 Score = 29.6 bits (65), Expect = 6.8
 Identities = 14/42 (33%), Positives = 20/42 (47%)
 Frame = -2

Query: 181 HLAKAARGSVGFPACFAGESKGVAPRR*AVREGADCELSRRS 56
           H +   +G  G PA    E  G   R  A  +G DCE++R +
Sbjct: 14  HASTVLQGCRGVPAAMWSEPAGAIRRHCATIDGMDCEVAREA 55



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>FLIP1_RAT (Q8K4T4) Filamin-A-interacting protein 1 (FILIP)|
          Length = 1212

 Score = 29.3 bits (64), Expect = 8.9
 Identities = 15/35 (42%), Positives = 19/35 (54%), Gaps = 3/35 (8%)
 Frame = +2

Query: 92   DGSSARRNPLRLP---GEAGGEPDAAPRGLGQMTR 187
            DGSS R  P R+P   G   G+P  A  G G +T+
Sbjct: 1148 DGSSQRPTPTRIPMSKGMKAGKPVVAASGAGNLTK 1182



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>PUR1_SHIFL (P0AG17) Amidophosphoribosyltransferase (EC 2.4.2.14) (Glutamine|
           phosphoribosylpyrophosphate amidotransferase) (ATASE)
           (GPATase)
          Length = 504

 Score = 29.3 bits (64), Expect = 8.9
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +2

Query: 227 KLPRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYP 340
           +L +K F   R H N  SDS   + I   ++D  +HYP
Sbjct: 109 ELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYP 146



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>PUR1_ECOLI (P0AG16) Amidophosphoribosyltransferase (EC 2.4.2.14) (Glutamine|
           phosphoribosylpyrophosphate amidotransferase) (ATASE)
           (GPATase)
          Length = 504

 Score = 29.3 bits (64), Expect = 8.9
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +2

Query: 227 KLPRKRFYRARAHSNPLSDSHFPIPISPDDVDLSQHYP 340
           +L +K F   R H N  SDS   + I   ++D  +HYP
Sbjct: 109 ELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYP 146



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>CDX2_MOUSE (P43241) Homeobox protein CDX-2 (Caudal-type homeobox protein 2)|
          Length = 311

 Score = 29.3 bits (64), Expect = 8.9
 Identities = 12/34 (35%), Positives = 22/34 (64%)
 Frame = -2

Query: 472 EERREPDEQPTKPAPDVSEADPGRLRLSAPVPVT 371
           +++++  +QP +P P  S+  PG LR S P P++
Sbjct: 249 QQQQQQQQQPPQPPPQPSQPQPGALR-SVPEPLS 281


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,847,998
Number of Sequences: 219361
Number of extensions: 745726
Number of successful extensions: 3053
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 2837
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3042
length of database: 80,573,946
effective HSP length: 106
effective length of database: 57,321,680
effective search space used: 5158951200
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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