ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart13h02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1CWC25_YARLI (Q6C1V6) Pre-mRNA-splicing factor CWC25 33 0.82
2VNS2_BTV1X (P10350) Nonstructural protein NS2 31 3.1
3SHK1_SCHPO (P50527) Serine/threonine-protein kinase pak1/shk1 (E... 31 3.1
4NSD1_MOUSE (O88491) Histone-lysine N-methyltransferase, H3 lysin... 30 5.3
5SPB4_ASPOR (Q2UBZ5) ATP-dependent rRNA helicase spb4 (EC 3.6.1.-) 30 5.3
6LEGA_PEA (P02857) Legumin A precursor [Contains: Legumin A alpha... 30 5.3
7LEU22_BRAJA (Q89X34) 3-isopropylmalate dehydratase large subunit... 30 6.9
8RCC2_XENLA (Q52KW8) Protein RCC2 homolog 29 9.1
9MMTA2_MOUSE (Q99LX5) Multiple myeloma tumor-associated protein 2... 29 9.1
10YNJ1_YEAST (P53935) Hypothetical 141.5 kDa protein in YPT53-RHO2... 29 9.1
11CE152_HUMAN (O94986) Centrosomal protein of 152 kDa (Cep152 prot... 29 9.1

>CWC25_YARLI (Q6C1V6) Pre-mRNA-splicing factor CWC25|
          Length = 561

 Score = 32.7 bits (73), Expect = 0.82
 Identities = 18/58 (31%), Positives = 30/58 (51%)
 Frame = -1

Query: 590 VAPDDQAYGDADDEPRAPEGVREDAGGDRRQEDQRARQL*PIREVARKE*PQDHNNER 417
           V+ DD+ Y  +  E R  +  RE+ G  R ++  R R     R+ +R+E  +D + ER
Sbjct: 228 VSDDDRGYRQSSREERDRKSSREEGGRSRSRDRYRNRSRERYRDRSREERSRDRSRER 285



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>VNS2_BTV1X (P10350) Nonstructural protein NS2|
          Length = 357

 Score = 30.8 bits (68), Expect = 3.1
 Identities = 24/98 (24%), Positives = 44/98 (44%), Gaps = 6/98 (6%)
 Frame = -1

Query: 599 VEQVAPDDQAYGDADDEPRAPEGVREDAGGDRRQEDQRARQL*PIREVAR------KE*P 438
           V+ VAP +++    DDE +  E  RE   G  R E  R  +    +EVA        E  
Sbjct: 173 VQSVAPREESRWMDDDEAKVDEEAREMIPGTSRLEKLREARSNVFKEVAAGINWNLDEKD 232

Query: 437 QDHNNERDPATNLPPYKGEEQRRHGEENVWDQETRQHV 324
           ++  +ER+    +     ++++  GE+   D+  + H+
Sbjct: 233 EEDGDEREDEERVKTLSDDDEQ--GEDASDDEHPKTHI 268



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>SHK1_SCHPO (P50527) Serine/threonine-protein kinase pak1/shk1 (EC 2.7.11.1)|
          Length = 658

 Score = 30.8 bits (68), Expect = 3.1
 Identities = 21/76 (27%), Positives = 36/76 (47%), Gaps = 1/76 (1%)
 Frame = -2

Query: 583 PMTRHTATPMMSH-EPLKVSAKMLGATAVRRIREPGSSSQSGKWRGKNSRRTTTTNAIQL 407
           P+   +A    SH +P   ++        R    P +SS S       + +TTT+NA + 
Sbjct: 226 PLLSVSALSSSSHLQPTSATSSSSRLYPSRPAPTPPASSSSSPLLSSQTVKTTTSNASRQ 285

Query: 406 PISPLIKEKSREGMVK 359
           P SPL+  KS + +++
Sbjct: 286 P-SPLVSSKSTDNIIR 300



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>NSD1_MOUSE (O88491) Histone-lysine N-methyltransferase, H3 lysine-36 and H4|
            lysine-20 specific (EC 2.1.1.43) (H3-K36-HMTase)
            (H4-K20-HMTase) (Nuclear receptor binding SET domain
            containing protein 1) (NR-binding SET domain containing
            protein)
          Length = 2588

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 26/99 (26%), Positives = 45/99 (45%), Gaps = 7/99 (7%)
 Frame = -2

Query: 583  PMTRHTATPMMSHEPLKVSAKMLGATAVRRIREPGSSSQ-------SGKWRGKNSRRTTT 425
            PMTR +++P +S  PL+   +M  +   + I      SQ       S   R  +  R  T
Sbjct: 2233 PMTRPSSSPSVSSLPLERPLRMTDSRLDKSIGAASPKSQAVEKTPASTGLRLSSPDRLLT 2292

Query: 424  TNAIQLPISPLIKEKSREGMVKKMYGTKRPVSMLVFQFV 308
            TN+ +  IS    EKS   + +++   ++ +S +V   V
Sbjct: 2293 TNSPKPQISDRPPEKSHASLTQRLPPPEKVLSAVVQSLV 2331



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>SPB4_ASPOR (Q2UBZ5) ATP-dependent rRNA helicase spb4 (EC 3.6.1.-)|
          Length = 638

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 22/84 (26%), Positives = 36/84 (42%)
 Frame = -1

Query: 581 DDQAYGDADDEPRAPEGVREDAGGDRRQEDQRARQL*PIREVARKE*PQDHNNERDPATN 402
           DD  Y D   E R  E ++E   GD  QE    R+       + K    D  N++     
Sbjct: 518 DDYKYKDKQREKRRIELLQESKEGDGTQESSNKRKATETTAWSNK---LDDRNKKQK--- 571

Query: 401 LPPYKGEEQRRHGEENVWDQETRQ 330
               + E+++R  E+N W++ T +
Sbjct: 572 ----RREQKQRRQEKNKWEKMTEE 591



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>LEGA_PEA (P02857) Legumin A precursor [Contains: Legumin A alpha chain|
           (Legumin A acidic chain); Legumin A beta chain (Legumin
           A basic chain)]
          Length = 517

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 20/69 (28%), Positives = 31/69 (44%)
 Frame = -1

Query: 539 PEGVREDAGGDRRQEDQRARQL*PIREVARKE*PQDHNNERDPATNLPPYKGEEQRRHGE 360
           PE       G R++ED+   +    +  +R+E  +D + ER P           QRR GE
Sbjct: 259 PEKQARHQRGSRQEEDEDEEKQPRHQRGSRQEEEEDEDEERQP---------RHQRRRGE 309

Query: 359 ENVWDQETR 333
           E   D++ R
Sbjct: 310 EEEEDKKER 318



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>LEU22_BRAJA (Q89X34) 3-isopropylmalate dehydratase large subunit 2 (EC|
           4.2.1.33) (Isopropylmalate isomerase 2) (Alpha-IPM
           isomerase 2) (IPMI 2)
          Length = 468

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/25 (52%), Positives = 16/25 (64%)
 Frame = -1

Query: 590 VAPDDQAYGDADDEPRAPEGVREDA 516
           VAPD +AY    D P+AP+G   DA
Sbjct: 235 VAPDQKAYDFLRDRPKAPKGAAWDA 259



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>RCC2_XENLA (Q52KW8) Protein RCC2 homolog|
          Length = 513

 Score = 29.3 bits (64), Expect = 9.1
 Identities = 14/55 (25%), Positives = 26/55 (47%)
 Frame = -2

Query: 481 GSSSQSGKWRGKNSRRTTTTNAIQLPISPLIKEKSREGMVKKMYGTKRPVSMLVF 317
           GS    G ++G N++R     A + P + ++ E        K+ G+K    +L+F
Sbjct: 46  GSPGSDGGFQGHNNKRGPAKGATKAPTAVIVTEPEHSKEKIKLEGSKAKGQLLIF 100



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>MMTA2_MOUSE (Q99LX5) Multiple myeloma tumor-associated protein 2 homolog|
          Length = 260

 Score = 29.3 bits (64), Expect = 9.1
 Identities = 15/75 (20%), Positives = 37/75 (49%)
 Frame = -1

Query: 551 EPRAPEGVREDAGGDRRQEDQRARQL*PIREVARKE*PQDHNNERDPATNLPPYKGEEQR 372
           +PRA + V  DA   ++ + ++ ++    ++  +K+  ++H  E D  ++ P       +
Sbjct: 168 KPRAEDKVEPDAESHKKSKKEKKKKKKKHKKHKKKK-DKEHKREADSCSSSPSPPRPRHQ 226

Query: 371 RHGEENVWDQETRQH 327
           RH + +   +  R+H
Sbjct: 227 RHSDFSPCSKRKREH 241



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>YNJ1_YEAST (P53935) Hypothetical 141.5 kDa protein in YPT53-RHO2 intergenic|
           region
          Length = 1240

 Score = 29.3 bits (64), Expect = 9.1
 Identities = 26/113 (23%), Positives = 44/113 (38%), Gaps = 4/113 (3%)
 Frame = -1

Query: 581 DDQAYGDADDEPRAPEGVREDAGGDRRQEDQRARQL*PIREVARKE*PQDHNNERDPATN 402
           D++ Y D  +     E V E  G +  ++ +   +   IRE        DH  +     N
Sbjct: 463 DEEDYDDYSEYAEDSEEVSEYEGIEAVEKPEHDEKSNGIRETLHLSYDHDHKRQ-----N 517

Query: 401 LPPYKGEEQRRHGEENVWDQETRQHVGLPVRLVQH----QEIYGGGEGEPLEK 255
            P +       H E+ + ++E    + LP    +H     +I  G E EP E+
Sbjct: 518 HPHHHYHSTSTHSEDELSEEEYISDIELPHDPHKHFHRDDDILDGDEDEPEEE 570



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>CE152_HUMAN (O94986) Centrosomal protein of 152 kDa (Cep152 protein)|
          Length = 1275

 Score = 29.3 bits (64), Expect = 9.1
 Identities = 18/83 (21%), Positives = 37/83 (44%)
 Frame = -2

Query: 553 MSHEPLKVSAKMLGATAVRRIREPGSSSQSGKWRGKNSRRTTTTNAIQLPISPLIKEKSR 374
           ++++P + SA+  G+ A              ++ G N         IQL +    KE+  
Sbjct: 100 VTYKPYQSSAQNNGSPAQEITGSDTFEGLQQQFLGANENSAENMQIIQLQVLNKAKERQL 159

Query: 373 EGMVKKMYGTKRPVSMLVFQFVL 305
           E +++K+  ++R +  L  Q V+
Sbjct: 160 ENLIEKLNESERQIRYLNHQLVI 182


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,674,252
Number of Sequences: 219361
Number of extensions: 1046549
Number of successful extensions: 3770
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 3664
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3763
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5253413348
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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