ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | bart11a09 |
|---|---|
| Clone Library Name | barley_pub |
>GUN1_PERAE (P05522) Endoglucanase 1 precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase) (Abscission cellulase 1) Length = 494 Score = 144 bits (364), Expect = 1e-34 Identities = 66/101 (65%), Positives = 82/101 (81%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFGF 409 +Y DAL KSILFFEGQRSG+LP +QR+ WR DS L+DG++ VDL GGYYDAGDN+KFG Sbjct: 29 HYSDALEKSILFFEGQRSGKLPTNQRLTWRGDSGLSDGSSYHVDLVGGYYDAGDNLKFGL 88 Query: 410 PMAFTTTLMSWGLIDFGKNFGSHGGEARAAVRWATDYLMKA 532 PMAFTTT+++WG+I+FG ARAA+RW+TDYL+KA Sbjct: 89 PMAFTTTMLAWGIIEFGCLMPEQVENARAALRWSTDYLLKA 129
>GUN9_ARATH (Q9C9H5) Probable family 9 endoglucanase At1g71380 precursor (EC| 3.2.1.4) Length = 484 Score = 138 bits (347), Expect = 1e-32 Identities = 62/100 (62%), Positives = 76/100 (76%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFGF 409 NY +AL KS+LFF+GQRSG LP Q++ WR S L+DG+ A VDLTGGYYDAGDNVKF Sbjct: 24 NYKEALSKSLLFFQGQRSGPLPRGQQISWRASSGLSDGSAAHVDLTGGYYDAGDNVKFNL 83 Query: 410 PMAFTTTLMSWGLIDFGKNFGSHGGEARAAVRWATDYLMK 529 PMAFTTT++SW +++GK G AR +RWATDYL+K Sbjct: 84 PMAFTTTMLSWSALEYGKRMGPELENARVNIRWATDYLLK 123
>GUN_PHAVU (P22503) Endoglucanase precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase) (Abscission cellulase) Length = 496 Score = 129 bits (325), Expect = 4e-30 Identities = 58/104 (55%), Positives = 79/104 (75%), Gaps = 2/104 (1%) Frame = +2 Query: 227 HNYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFG 406 ++Y DAL K+ILFFEGQRSG+LP QRV+WR DSAL+DG V+L GGYYDAGDNVKFG Sbjct: 39 YDYADALAKAILFFEGQRSGKLPSSQRVKWREDSALSDGKLQNVNLMGGYYDAGDNVKFG 98 Query: 407 FPMAFTTTLMSWGLIDFGKNFGSHG--GEARAAVRWATDYLMKA 532 +PMAF+T+L+SW +++ S G ++A+RW D++++A Sbjct: 99 WPMAFSTSLLSWAAVEYESEISSVNQLGYLQSAIRWGADFMLRA 142
>GUN4_THEFU (P26221) Endoglucanase E-4 precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase E-4) (Cellulase E-4) (Cellulase E4) Length = 880 Score = 123 bits (309), Expect = 3e-28 Identities = 57/103 (55%), Positives = 74/103 (71%), Gaps = 2/103 (1%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFGF 409 NY +AL KS+ F+E QRSG+LP + RV WR DS LNDGA G+DLTGG+YDAGD+VKFGF Sbjct: 51 NYAEALQKSMFFYEAQRSGKLPENNRVSWRGDSGLNDGADVGLDLTGGWYDAGDHVKFGF 110 Query: 410 PMAFTTTLMSWGLIDFGKNFGSHG--GEARAAVRWATDYLMKA 532 PMAFT T+++WG I+ + + G + +RW DY +KA Sbjct: 111 PMAFTATMLAWGAIESPEGYIRSGQMPYLKDNLRWVNDYFIKA 153
>GUNB_CELFI (P26225) Endoglucanase B precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase B) (Cellulase B) Length = 1045 Score = 115 bits (288), Expect = 7e-26 Identities = 54/104 (51%), Positives = 72/104 (69%), Gaps = 2/104 (1%) Frame = +2 Query: 227 HNYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFG 406 +NY +AL KS+ F++ QRSG LP D V WR DS L DGA G DLTGG+YDAGD+VKFG Sbjct: 37 YNYAEALQKSMFFYQAQRSGDLPADFPVSWRGDSGLTDGADVGKDLTGGWYDAGDHVKFG 96 Query: 407 FPMAFTTTLMSWGLIDFGKNFGSHGG--EARAAVRWATDYLMKA 532 FPMAF+ T+++WG I+ + G E + +R+ +DY +KA Sbjct: 97 FPMAFSATMLAWGAIESPTGYSKAGSLDELKDNLRFVSDYFVKA 140
>GUNZ_CLOSR (P23659) Endoglucanase Z precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase) (Thermoactive cellulase) (Avicelase I) Length = 986 Score = 115 bits (287), Expect = 1e-25 Identities = 52/103 (50%), Positives = 74/103 (71%), Gaps = 2/103 (1%) Frame = +2 Query: 227 HNYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFG 406 +NYG+AL K+I+F+E QRSG+LP ++R WR DS LNDGA G+DLTGG+YDAGD+VKF Sbjct: 30 YNYGEALQKAIMFYEFQRSGKLPENKRDNWRGDSGLNDGADVGLDLTGGWYDAGDHVKFN 89 Query: 407 FPMAFTTTLMSWGLIDFGKNFGSHG--GEARAAVRWATDYLMK 529 PMA++ T+++W + + G G A++W +DYL+K Sbjct: 90 LPMAYSQTMLAWAAYEAEEALERSGQMGYLLDAIKWVSDYLIK 132
>GUNF_CLOTM (P26224) Endoglucanase F precursor (EC 3.2.1.4) (EGF)| (Endo-1,4-beta-glucanase) (Cellulase F) Length = 739 Score = 112 bits (281), Expect = 5e-25 Identities = 52/102 (50%), Positives = 72/102 (70%), Gaps = 2/102 (1%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFGF 409 NYG+AL K+I+F+E QRSG+LP ++R WR DSALNDGA G+DLTGG+YDAGD+VKF Sbjct: 31 NYGEALQKAIMFYEFQRSGKLPENKRNNWRGDSALNDGADNGLDLTGGWYDAGDHVKFNL 90 Query: 410 PMAFTTTLMSWGLIDFGKNFGSHGGEARAA--VRWATDYLMK 529 PMA+ T+++W + + + G ++WATDY +K Sbjct: 91 PMAYAVTMLAWSVYESRDAYVQSGQLPYILDNIKWATDYFIK 132
>GUNG_CLOCE (P37700) Endoglucanase G precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase G) (Cellulase G) (EGCCG) Length = 725 Score = 112 bits (279), Expect = 8e-25 Identities = 51/104 (49%), Positives = 73/104 (70%), Gaps = 3/104 (2%) Frame = +2 Query: 227 HNYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFG 406 +NYG+AL KSI+F+E QRSG LP D+R WR DS + DG+ GVDLTGG+YDAGD+VKF Sbjct: 39 YNYGEALQKSIMFYEFQRSGDLPADKRDNWRDDSGMKDGSDVGVDLTGGWYDAGDHVKFN 98 Query: 407 FPMAFTTTLMSWGLIDFGKNFGSHGGEAR---AAVRWATDYLMK 529 PM++T+ +++W L + K+ G+ + ++WA DY +K Sbjct: 99 LPMSYTSAMLAWSLYE-DKDAYDKSGQTKYIMDGIKWANDYFIK 141
>GUN4_BACS5 (P28622) Endoglucanase 4 precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase 4) (Cellulase 4) (EG-IV) Length = 636 Score = 108 bits (271), Expect = 7e-24 Identities = 49/104 (47%), Positives = 70/104 (67%), Gaps = 2/104 (1%) Frame = +2 Query: 227 HNYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFG 406 +NY + L KSILF+E QRSG+LP R+ WR DS L DG G DLTGG+YDAGD+VKFG Sbjct: 28 YNYVEVLQKSILFYEAQRSGKLPESNRLNWRGDSGLEDGKDVGHDLTGGWYDAGDHVKFG 87 Query: 407 FPMAFTTTLMSWGLIDFGKNFGSHG--GEARAAVRWATDYLMKA 532 PMA++ +++W + ++ + + + ++WATDY +KA Sbjct: 88 LPMAYSAAVLAWTVYEYREAYEEAELLDDMLDQIKWATDYFLKA 131
>GUNI_CLOTM (Q02934) Endoglucanase 1 precursor (EC 3.2.1.4) (Endoglucanase I)| (EGI) (Endo-1,4-beta-glucanase) (Cellulase I) Length = 879 Score = 104 bits (260), Expect = 1e-22 Identities = 48/103 (46%), Positives = 68/103 (66%), Gaps = 3/103 (2%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLPPDQ-RVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFG 406 NYG+AL K+I F+E QRSG+L P R+ WR DS L+DG AG+DLTGG+YDAGD+VKF Sbjct: 77 NYGEALQKAIFFYECQRSGKLDPSTLRLNWRGDSGLDDGKDAGIDLTGGWYDAGDHVKFN 136 Query: 407 FPMAFTTTLMSWGLIDFGKNFGSHG--GEARAAVRWATDYLMK 529 PM+++ ++ W + ++ F G ++WA DY +K Sbjct: 137 LPMSYSAAMLGWAVYEYEDAFKQSGQYNHILNNIKWACDYFIK 179
>GUNA_CALSA (P22534) Endoglucanase A precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase A) (Cellulase A) Length = 1742 Score = 101 bits (251), Expect = 1e-21 Identities = 48/102 (47%), Positives = 65/102 (63%), Gaps = 2/102 (1%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATAGVDLTGGYYDAGDNVKFGF 409 NYG+AL K+I+F+E Q SG+LP R WR DSAL DG G+DLTGG++DAGD+VKF Sbjct: 27 NYGEALQKAIMFYEFQMSGKLPNWVRNNWRGDSALKDGQDNGLDLTGGWFDAGDHVKFNL 86 Query: 410 PMAFTTTLMSWGLIDFGKNFGSHG--GEARAAVRWATDYLMK 529 PM++T T++SW ++ F G + W DY +K Sbjct: 87 PMSYTGTMLSWAAYEYKDAFVKSGQLEHILNQIEWVNDYFVK 128
>GUN6_DICDI (P22699) Endoglucanase precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase) (Spore germination protein 270-6) (Cellulase) Length = 705 Score = 79.7 bits (195), Expect = 5e-15 Identities = 41/110 (37%), Positives = 65/110 (59%), Gaps = 9/110 (8%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDG-------ATAGVDLTGGYYDAG 388 +Y L +++F++ R+GRLP D + WR +SALND A +L+GGY+DAG Sbjct: 26 DYCSLLENALMFYKMNRAGRLP-DNDIPWRGNSALNDASPNSAKDANGDGNLSGGYFDAG 84 Query: 389 DNVKFGFPMAFTTTLMSWGLIDFGKNFGSHGGEA--RAAVRWATDYLMKA 532 D VKFG PMA++ T++ W I++ N G + +++ TD+L+ A Sbjct: 85 DGVKFGLPMAYSMTMLGWSFIEYESNIAQCGLTSLYLDTIKYGTDWLIAA 134
>GUNA_FIBSU (P23665) Endoglucanase A precursor (EC 3.2.1.4)| (Endo-1,4-beta-glucanase) (Cellulase) Length = 453 Score = 37.4 bits (85), Expect = 0.026 Identities = 22/81 (27%), Positives = 39/81 (48%), Gaps = 5/81 (6%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLPPDQRVRWRRDSALNDGATA-----GVDLTGGYYDAGDN 394 +Y +A + FF QRSG+ P W D N + G D++GG++D GD+ Sbjct: 29 DYVEAAWMTTRFFGAQRSGQGP-----NWILDGTSNPTSFTKDSYNGKDVSGGWFDCGDH 83 Query: 395 VKFGFPMAFTTTLMSWGLIDF 457 V +G + + +++ +F Sbjct: 84 VMYGQSQGYASYVLALAYAEF 104
>YCEB_CALSA (P21481) Hypothetical protein in celB 3'region (Fragment)| Length = 66 Score = 35.0 bits (79), Expect = 0.13 Identities = 14/22 (63%), Positives = 19/22 (86%) Frame = +2 Query: 230 NYGDALHKSILFFEGQRSGRLP 295 NYG+AL K+I+F+E Q SG+LP Sbjct: 42 NYGEALQKAIMFYEFQMSGKLP 63
>CWC21_USTMA (Q4P0G6) Pre-mRNA-splicing factor CWC21| Length = 348 Score = 30.8 bits (68), Expect = 2.4 Identities = 15/37 (40%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = +3 Query: 12 RSPLQCSSSPAPEP---PRTHSANRLNCRPSTPEKPS 113 RSP +C+SS + P PR HS++R+N P +P+ Sbjct: 310 RSPSRCASSRSRSPAHRPRRHSSSRVNPPGPPPRRPA 346
>GUNC_BUTFI (P23658) Cellodextrinase (EC 3.2.1.-)| Length = 547 Score = 30.0 bits (66), Expect = 4.1 Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 12/70 (17%) Frame = +2 Query: 356 VDLTGGYYDAGDNVKFGFPMAFTTTLMSWGL----------IDFGKNFGSHGG--EARAA 499 VD+TGG++DAGD ++ A + +G+ D K G G E A Sbjct: 137 VDVTGGWHDAGDYGRYSTAGAVAVAHLLYGVRFFKGLLDVHYDIPKVAGDKGNLPEILAE 196 Query: 500 VRWATDYLMK 529 V+ D+LMK Sbjct: 197 VKVELDFLMK 206
>GYRA_AERSA (P48369) DNA gyrase subunit A (EC 5.99.1.3)| Length = 922 Score = 30.0 bits (66), Expect = 4.1 Identities = 24/85 (28%), Positives = 38/85 (44%), Gaps = 8/85 (9%) Frame = +2 Query: 278 RSGRLPPDQRVRWRRDSALND-------GATAGVDLTGGYYDAGDNVKFGFPMAFTTTL- 433 R G P +RV + + ND A D+ G Y+ GD+ + + Sbjct: 38 RDGLKPVHRRVLFAMNELGNDWNKPYKKSARVVGDVIGKYHPHGDSAVYDTIVRLAQDFS 97 Query: 434 MSWGLIDFGKNFGSHGGEARAAVRW 508 M + L+D NFGS G++ AA+R+ Sbjct: 98 MRYMLVDGQGNFGSVDGDSAAAMRY 122
>GYRA_HELPY (P48370) DNA gyrase subunit A (EC 5.99.1.3)| Length = 827 Score = 29.6 bits (65), Expect = 5.4 Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 1/59 (1%) Frame = +2 Query: 359 DLTGGYYDAGDNVKFGFPMAFTTTL-MSWGLIDFGKNFGSHGGEARAAVRWATDYLMKA 532 D+ G Y+ GDN + + M L+D NFGS G+ AA+R+ + KA Sbjct: 76 DVIGKYHPHGDNAVYDALVRMAQDFSMRLELVDGQGNFGSIDGDNAAAMRYTEARMTKA 134
>LRFN3_MOUSE (Q8BLY3) Leucine-rich repeat and fibronectin type-III| domain-containing protein 3 precursor Length = 626 Score = 29.3 bits (64), Expect = 7.1 Identities = 13/25 (52%), Positives = 17/25 (68%), Gaps = 2/25 (8%) Frame = +3 Query: 33 SSPAPEP--PRTHSANRLNCRPSTP 101 S+PAPEP PR H+ +L+C P P Sbjct: 595 SAPAPEPAAPRAHTVVQLDCEPWGP 619
>GYRA_SALTY (P37411) DNA gyrase subunit A (EC 5.99.1.3)| Length = 878 Score = 29.3 bits (64), Expect = 7.1 Identities = 25/92 (27%), Positives = 40/92 (43%), Gaps = 8/92 (8%) Frame = +2 Query: 278 RSGRLPPDQRVRWRRDSALND-------GATAGVDLTGGYYDAGDNVKFGFPMAFTTTL- 433 R G P +RV + + ND A D+ G Y+ GD+ + + Sbjct: 38 RDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFS 97 Query: 434 MSWGLIDFGKNFGSHGGEARAAVRWATDYLMK 529 + + L+D NFGS G++ AA+R+ L K Sbjct: 98 LRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAK 129
>GYRA_SHIFL (P0AES5) DNA gyrase subunit A (EC 5.99.1.3)| Length = 875 Score = 29.3 bits (64), Expect = 7.1 Identities = 25/92 (27%), Positives = 40/92 (43%), Gaps = 8/92 (8%) Frame = +2 Query: 278 RSGRLPPDQRVRWRRDSALND-------GATAGVDLTGGYYDAGDNVKFGFPMAFTTTL- 433 R G P +RV + + ND A D+ G Y+ GD+ + + Sbjct: 38 RDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFS 97 Query: 434 MSWGLIDFGKNFGSHGGEARAAVRWATDYLMK 529 + + L+D NFGS G++ AA+R+ L K Sbjct: 98 LRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAK 129
>GYRA_ECOLI (P0AES4) DNA gyrase subunit A (EC 5.99.1.3)| Length = 875 Score = 29.3 bits (64), Expect = 7.1 Identities = 25/92 (27%), Positives = 40/92 (43%), Gaps = 8/92 (8%) Frame = +2 Query: 278 RSGRLPPDQRVRWRRDSALND-------GATAGVDLTGGYYDAGDNVKFGFPMAFTTTL- 433 R G P +RV + + ND A D+ G Y+ GD+ + + Sbjct: 38 RDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFS 97 Query: 434 MSWGLIDFGKNFGSHGGEARAAVRWATDYLMK 529 + + L+D NFGS G++ AA+R+ L K Sbjct: 98 LRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAK 129
>GYRA_BUCAP (Q8K9W2) DNA gyrase subunit A (EC 5.99.1.3)| Length = 825 Score = 28.9 bits (63), Expect = 9.2 Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 1/51 (1%) Frame = +2 Query: 359 DLTGGYYDAGDNVKFGFPMAFTTTL-MSWGLIDFGKNFGSHGGEARAAVRW 508 D+ G Y+ GD+ + + + + LID NFGS G+A AA+R+ Sbjct: 72 DVIGKYHPHGDSAVYDSIVRMAQNFSLRYMLIDGQGNFGSIDGDAAAAMRY 122
>GYRA_HAEIN (P43700) DNA gyrase subunit A (EC 5.99.1.3)| Length = 880 Score = 28.9 bits (63), Expect = 9.2 Identities = 25/92 (27%), Positives = 39/92 (42%), Gaps = 8/92 (8%) Frame = +2 Query: 278 RSGRLPPDQRVRWRRDSALNDGATAGV-------DLTGGYYDAGDNVKFGFPMAFTTTL- 433 R G P +RV + D N V D+ G Y+ GD+ + + Sbjct: 39 RDGLKPVHRRVLFSMDREGNTANKKYVKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFS 98 Query: 434 MSWGLIDFGKNFGSHGGEARAAVRWATDYLMK 529 + + L+D NFGS G+A AA+R+ + K Sbjct: 99 LRYMLVDGQGNFGSIDGDAPAAMRYTEVRMQK 130 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 38,011,158 Number of Sequences: 219361 Number of extensions: 675551 Number of successful extensions: 3145 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 2939 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3130 length of database: 80,573,946 effective HSP length: 105 effective length of database: 57,541,041 effective search space used: 4085413911 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
