ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | bart10e06 |
|---|---|
| Clone Library Name | barley_pub |
>EBP_ORYSA (Q9FTZ2) Probable 3-beta-hydroxysteroid-delta(8),delta(7)-isomerase| (EC 5.3.3.5) (Cholestenol delta-isomerase) (Delta8-delta7 sterol isomerase) (D8-D7 sterol isomerase) Length = 219 Score = 216 bits (551), Expect = 3e-56 Identities = 107/144 (74%), Positives = 116/144 (80%), Gaps = 1/144 (0%) Frame = +3 Query: 120 HPYVPASLDLPGYVPLRLTQLEILAAYLGTSXXXXXXXXXXSGRC-RRLSGADRLLMCWW 296 HPY PA L LPG+VPL+L+Q +IL YL TS SGRC RRLS DR LMCWW Sbjct: 7 HPYAPAELHLPGFVPLQLSQAQILVPYLATSLFLLLAVWLISGRCSRRLSDTDRWLMCWW 66 Query: 297 AFTGLTHILIEGPFVFTPGFFTKTSPNFFDEVWKEYSKGDSRYVARDTATVTVEGITAVL 476 AFTGLTHI+IEG FVF P FF+ +P++FDEVWKEYSKGDSRYVARD ATVTVEGITAVL Sbjct: 67 AFTGLTHIIIEGTFVFAPNFFSNQNPSYFDEVWKEYSKGDSRYVARDPATVTVEGITAVL 126 Query: 477 EGPASLLAVYAIASRKSYSHILQF 548 EGPASLLAVYAIAS KSYSHILQF Sbjct: 127 EGPASLLAVYAIASGKSYSHILQF 150
>EBP_ARATH (O48962) Probable 3-beta-hydroxysteroid-delta(8),delta(7)-isomerase| (EC 5.3.3.5) (Cholestenol delta-isomerase) (Delta8-delta7 sterol isomerase) (D8-D7 sterol isomerase) Length = 223 Score = 174 bits (441), Expect = 1e-43 Identities = 83/143 (58%), Positives = 105/143 (73%) Frame = +3 Query: 117 AHPYVPASLDLPGYVPLRLTQLEILAAYLGTSXXXXXXXXXXSGRCRRLSGADRLLMCWW 296 AHPYVP L+LPGYVP+ ++ I++ YLG+S GR + + D+LLMCWW Sbjct: 5 AHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLMCWW 62 Query: 297 AFTGLTHILIEGPFVFTPGFFTKTSPNFFDEVWKEYSKGDSRYVARDTATVTVEGITAVL 476 FTGLTH+++EG FVF+P FF + + EVWKEYSKGDSRYV RD+A V+VEGITAV+ Sbjct: 63 TFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGITAVI 122 Query: 477 EGPASLLAVYAIASRKSYSHILQ 545 GPASLLA+YAIA KSYS++LQ Sbjct: 123 VGPASLLAIYAIAKEKSYSYVLQ 145
>EBP_MOUSE (P70245) 3-beta-hydroxysteroid-delta(8),delta(7)-isomerase (EC| 5.3.3.5) (Cholestenol delta-isomerase) (Delta8-delta7 sterol isomerase) (D8-D7 sterol isomerase) (Emopamil-binding protein) Length = 229 Score = 87.0 bits (214), Expect = 3e-17 Identities = 52/144 (36%), Positives = 68/144 (47%), Gaps = 2/144 (1%) Frame = +3 Query: 120 HPYVPASLDLPGYVPLRLTQLEILAAYLGTSXXXXXXXXXXSGRCR--RLSGADRLLMCW 293 HPY P L L +VP L IL S S R L RL +CW Sbjct: 8 HPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLIVITWLLSSRASVVPLGAGRRLALCW 67 Query: 294 WAFTGLTHILIEGPFVFTPGFFTKTSPNFFDEVWKEYSKGDSRYVARDTATVTVEGITAV 473 +A H++IEG F G + F ++WKEYSKGDSRY+ D+ V +E +TA Sbjct: 68 FAVCTFIHLVIEGWFSLYNGILLEDQA-FLSQLWKEYSKGDSRYILSDSFVVCMETVTAC 126 Query: 474 LEGPASLLAVYAIASRKSYSHILQ 545 L GP SL V A ++ + +LQ Sbjct: 127 LWGPLSLWVVIAFLRQQPFRFVLQ 150
>EBP_HUMAN (Q15125) 3-beta-hydroxysteroid-delta(8),delta(7)-isomerase (EC| 5.3.3.5) (Cholestenol delta-isomerase) (Delta8-delta7 sterol isomerase) (D8-D7 sterol isomerase) (Emopamil-binding protein) Length = 229 Score = 83.6 bits (205), Expect = 3e-16 Identities = 55/144 (38%), Positives = 67/144 (46%), Gaps = 2/144 (1%) Frame = +3 Query: 120 HPYVPASLDLPGYVPLRLTQLEILAAYLGTSXXXXXXXXXXSGRCR--RLSGADRLLMCW 293 HPY P L L +VP ILA + SGR L RL +CW Sbjct: 8 HPYWPQHLRLDNFVPNDRPTWHILAGLFSVTGVLVVTTWLLSGRAAVVPLGTWRRLSLCW 67 Query: 294 WAFTGLTHILIEGPFVFTPGFFTKTSPNFFDEVWKEYSKGDSRYVARDTATVTVEGITAV 473 +A G H++IEG FV F ++WKEY+KGDSRY+ D TV +E ITA Sbjct: 68 FAVCGFIHLVIEGWFVLYYEDLLGDQA-FLSQLWKEYAKGDSRYILGDNFTVCMETITAC 126 Query: 474 LEGPASLLAVYAIASRKSYSHILQ 545 L GP SL V A + ILQ Sbjct: 127 LWGPLSLWVVIAFLRQHPLRFILQ 150
>EBP_RAT (Q9JJ46) 3-beta-hydroxysteroid-delta(8),delta(7)-isomerase (EC| 5.3.3.5) (Cholestenol delta-isomerase) (Delta8-delta7 sterol isomerase) (D8-D7 sterol isomerase) (Emopamil-binding protein) Length = 229 Score = 83.2 bits (204), Expect = 4e-16 Identities = 52/144 (36%), Positives = 66/144 (45%), Gaps = 2/144 (1%) Frame = +3 Query: 120 HPYVPASLDLPGYVPLRLTQLEILAAYLGTSXXXXXXXXXXSGRCR--RLSGADRLLMCW 293 HPY P L L +VP L IL S S R L RL +CW Sbjct: 8 HPYWPRHLRLDNFVPNDLPTWHILVGLFSFSGVLIVITWLLSSRVSVVPLGTGRRLALCW 67 Query: 294 WAFTGLTHILIEGPFVFTPGFFTKTSPNFFDEVWKEYSKGDSRYVARDTATVTVEGITAV 473 +A H++IEG F F + F ++WKEYSKGDSRY+ D V +E +TA Sbjct: 68 FAVCTFIHLVIEGWFSFYHEILLEDQA-FLSQLWKEYSKGDSRYILSDGFIVCMESVTAC 126 Query: 474 LEGPASLLAVYAIASRKSYSHILQ 545 L GP SL V A + + +LQ Sbjct: 127 LWGPLSLWVVIAFLRHQPFRFVLQ 150
>EBP_CAVPO (Q60490) 3-beta-hydroxysteroid-delta(8),delta(7)-isomerase (EC| 5.3.3.5) (Cholestenol delta-isomerase) (Delta8-delta7 sterol isomerase) (D8-D7 sterol isomerase) (Emopamil-binding protein) Length = 228 Score = 80.5 bits (197), Expect = 3e-15 Identities = 51/145 (35%), Positives = 67/145 (46%), Gaps = 2/145 (1%) Frame = +3 Query: 120 HPYVPASLDLPGYVPLRLTQLEILAAYLGTSXXXXXXXXXXSGRCR--RLSGADRLLMCW 293 HPY P L L +VP L+ I+ S R L RL +CW Sbjct: 9 HPYWPRHLRLDHFVPNDLSAWYIVTVLFTVFGALVVTMWLLSSRASVVPLGTWRRLSVCW 68 Query: 294 WAFTGLTHILIEGPFVFTPGFFTKTSPNFFDEVWKEYSKGDSRYVARDTATVTVEGITAV 473 +A H++IEG FV F ++WKEY+KGDSRY+ D + +E IT V Sbjct: 69 FAVCAFVHLVIEGWFVLYQKAILGDQA-FLSQLWKEYAKGDSRYIIEDNFIICMESITVV 127 Query: 474 LEGPASLLAVYAIASRKSYSHILQF 548 L GP SL AV A + ++LQF Sbjct: 128 LWGPLSLWAVIAFLRQHPSRYVLQF 152
>EBPL_HUMAN (Q9BY08) Emopamil-binding protein-like (Emopamil-binding-related| protein) Length = 206 Score = 70.5 bits (171), Expect = 3e-12 Identities = 37/98 (37%), Positives = 49/98 (50%), Gaps = 1/98 (1%) Frame = +3 Query: 255 RRLSGADRLLMCWWAFTGLTHILIEGPFVFTPGFFT-KTSPNFFDEVWKEYSKGDSRYVA 431 R ADR + W + L H +EGPFV+ S +WKEY K D+R+V Sbjct: 34 RGQGAADRGALIWLCYDALVHFALEGPFVYLSLVGNVANSDGLIASLWKEYGKADARWVY 93 Query: 432 RDTATVTVEGITAVLEGPASLLAVYAIASRKSYSHILQ 545 D V+VE +T L+G +L +YAI K Y H LQ Sbjct: 94 FDPTIVSVEILTVALDGSLALFLIYAIVKEKYYRHFLQ 131
>EBPL_MOUSE (Q9D0P0) Emopamil-binding protein-like (Emopamil-binding-related| protein) Length = 206 Score = 67.0 bits (162), Expect = 3e-11 Identities = 33/98 (33%), Positives = 52/98 (53%), Gaps = 1/98 (1%) Frame = +3 Query: 255 RRLSGADRLLMCWWAFTGLTHILIEGPFVFTPGFFTKT-SPNFFDEVWKEYSKGDSRYVA 431 R S +R ++ W + L H ++EG FV+ S +WKEY K D+R++ Sbjct: 34 RGRSAVERWVLAWLCYDSLVHFVLEGAFVYLSIVGNVADSQGLIASLWKEYGKADTRWLY 93 Query: 432 RDTATVTVEGITAVLEGPASLLAVYAIASRKSYSHILQ 545 D V++E +T VL+G +L+ +YAI K Y H +Q Sbjct: 94 SDPTVVSLEILTVVLDGLLALVLIYAIVKEKYYRHFVQ 131
>IRS1_CERAE (Q28224) Insulin receptor substrate 1 (IRS-1)| Length = 1251 Score = 31.2 bits (69), Expect = 2.0 Identities = 20/65 (30%), Positives = 24/65 (36%) Frame = +2 Query: 167 AAHPARDPGGIXXXXXXXXXXXXXXXXEMPQAVRGRPPAHVLVGLHRPHPHTHRGALRLY 346 A PA GG P +V G P V +R H H HRG+ RL+ Sbjct: 310 ATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSP---VSPSTNRTHAHRHRGSARLH 366 Query: 347 PRFLH 361 P H Sbjct: 367 PPLNH 371
>IRS1_HUMAN (P35568) Insulin receptor substrate 1 (IRS-1)| Length = 1242 Score = 31.2 bits (69), Expect = 2.0 Identities = 20/65 (30%), Positives = 24/65 (36%) Frame = +2 Query: 167 AAHPARDPGGIXXXXXXXXXXXXXXXXEMPQAVRGRPPAHVLVGLHRPHPHTHRGALRLY 346 A PA GG P +V G P V +R H H HRG+ RL+ Sbjct: 310 ATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSP---VSPSTNRTHAHRHRGSARLH 366 Query: 347 PRFLH 361 P H Sbjct: 367 PPLNH 371
>IRS1_RAT (P35570) Insulin receptor substrate 1 (IRS-1) (pp185)| Length = 1235 Score = 30.8 bits (68), Expect = 2.6 Identities = 20/65 (30%), Positives = 24/65 (36%) Frame = +2 Query: 167 AAHPARDPGGIXXXXXXXXXXXXXXXXEMPQAVRGRPPAHVLVGLHRPHPHTHRGALRLY 346 A PA GG P +V G P V +R H H HRG+ RL+ Sbjct: 305 ATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSP---VSPSTNRTHAHRHRGSSRLH 361 Query: 347 PRFLH 361 P H Sbjct: 362 PPLNH 366
>IRS1_MOUSE (P35569) Insulin receptor substrate 1 (IRS-1)| Length = 1233 Score = 30.8 bits (68), Expect = 2.6 Identities = 20/65 (30%), Positives = 24/65 (36%) Frame = +2 Query: 167 AAHPARDPGGIXXXXXXXXXXXXXXXXEMPQAVRGRPPAHVLVGLHRPHPHTHRGALRLY 346 A PA GG P +V G P V +R H H HRG+ RL+ Sbjct: 305 ATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSP---VSPSTNRTHAHRHRGSSRLH 361 Query: 347 PRFLH 361 P H Sbjct: 362 PPLNH 366
>GUAA_RHOPA (P60501) GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)| (Glutamine amidotransferase) (GMP synthetase) Length = 540 Score = 30.4 bits (67), Expect = 3.4 Identities = 17/51 (33%), Positives = 25/51 (49%) Frame = +3 Query: 381 FDEVWKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLAVYAIASRKSYS 533 FD +W++ K D D T G AV + P S ++V A +RK Y+ Sbjct: 142 FDGIWQKGGKYDVWMSHGDRVTKLPAGFRAVAQAPGSPISVIADDTRKFYA 192
>GUAA_NITWN (Q3SQP4) GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)| (Glutamine amidotransferase) (GMP synthetase) Length = 535 Score = 29.3 bits (64), Expect = 7.7 Identities = 17/51 (33%), Positives = 25/51 (49%) Frame = +3 Query: 381 FDEVWKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLAVYAIASRKSYS 533 FD VW++ D D T +G AV + P S +AV A +R+ Y+ Sbjct: 137 FDGVWEKGGTYDVWMSHGDRVTKLPDGFRAVAKAPGSPIAVIADDARRFYA 187
>CHCH6_MOUSE (Q91VN4) Coiled-coil-helix-coiled-coil-helix domain-containing| protein 6 Length = 273 Score = 29.3 bits (64), Expect = 7.7 Identities = 18/62 (29%), Positives = 27/62 (43%) Frame = -3 Query: 548 ELEDMAVRLARCDRIDREQRGRAFQHGRDSFXXXXXXXXXXXXXVTLAVLLPDFVEEVGA 369 ELED L+RCD +EQ+GR + + + + + P VE V + Sbjct: 178 ELEDREAELSRCDTFYKEQQGRIQEKNAELYKLSSQQFHEAASKAE-STIKPRRVEPVCS 236 Query: 368 GL 363 GL Sbjct: 237 GL 238
>YGGF_ECOLI (P21437) Hypothetical protein yggF| Length = 321 Score = 29.3 bits (64), Expect = 7.7 Identities = 19/49 (38%), Positives = 24/49 (48%) Frame = +3 Query: 390 VWKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLAVYAIASRKSYSH 536 + +E KGD V D A +EG V G ++ LAV A A R S H Sbjct: 67 IGEEVGKGDGPEV--DIAVDPIEGTRMVAMGQSNALAVMAFAPRDSLLH 113
>GPR18_HUMAN (Q14330) Probable G-protein coupled receptor 18| Length = 331 Score = 28.9 bits (63), Expect = 10.0 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = -1 Query: 112 IAAAGGVSIWEPEMEKEMEQICAGQLGCAAVWI 14 I+A ++I +P+ KE++ C L C VWI Sbjct: 115 ISADRYMAIVQPKYAKELKNTCKAVLACVGVWI 147
>PYRD_AERPE (Q9YFI6) Dihydroorotate dehydrogenase (EC 1.3.3.1) (Dihydroorotate| oxidase) (DHOdehase) (DHODase) (DHOD) Length = 309 Score = 28.9 bits (63), Expect = 10.0 Identities = 16/36 (44%), Positives = 20/36 (55%) Frame = -1 Query: 118 APIAAAGGVSIWEPEMEKEMEQICAGQLGCAAVWIG 11 A I AAGGV WE +E + A Q+G A + IG Sbjct: 241 ADIFAAGGVESWEDAVEFYLAGAKAVQVGAAFITIG 276
>DUSC_AZOVI (P96192) tRNA-dihydrouridine synthase C (EC 1.-.-.-)| Length = 309 Score = 28.9 bits (63), Expect = 10.0 Identities = 24/82 (29%), Positives = 38/82 (46%), Gaps = 3/82 (3%) Frame = -1 Query: 274 SAPDSLRHLPERSQTAATTRREVPRYAARISSWVSRSGT*PGRSRLAGT*GCAPIA-AAG 98 S PD R + R+ A ++RR RY S W + SG PG +G+ P+A ++ Sbjct: 225 SRPDLARQIAMRAPDARSSRRAGVRY----SRWCASSGGAPG----SGSRRATPLAGSSS 276 Query: 97 GVSIWEPEMEK--EMEQICAGQ 38 G + W + + CAG+ Sbjct: 277 GWACWRAVIRRRPRCSPNCAGR 298 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.312 0.117 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 64,754,868 Number of Sequences: 219361 Number of extensions: 1124247 Number of successful extensions: 4509 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 4267 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4483 length of database: 80,573,946 effective HSP length: 105 effective length of database: 57,541,041 effective search space used: 4430660157 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits)
