ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | basd20d04 |
|---|---|
| Clone Library Name | barley_pub |
>LYPA3_HUMAN (Q8NCC3) 1-O-acylceramide synthase precursor (EC 2.3.1.-) (ACS)| (Lysosomal phospholipase A2) (Lysophospholipase 3) (LPLA2) (LCAT-like lysophospholipase) (LLPL) Length = 412 Score = 37.0 bits (84), Expect = 0.043 Identities = 35/171 (20%), Positives = 70/171 (40%), Gaps = 18/171 (10%) Frame = +2 Query: 2 GKTLFGFGYDFRQS-NRLSETLDKFSKKLESVYTASGGKKINLITHSMGGLLVKCFMSLH 178 G+ + G YD+R++ N + +E +Y GG + L+ HSMG + F+ Sbjct: 153 GEDVRGAPYDWRRAPNENGPYFLALREMIEEMYQLYGGPVV-LVAHSMGNMYTLYFLQRQ 211 Query: 179 GDVF-EKYVKSWIAIAAPFQG--------APGYINSALLNGMSFVEGWQSKFFISKWTM- 328 + +KY+++++++ AP+ G A G N + G + Q + W + Sbjct: 212 PQAWKDKYIRAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLP 271 Query: 329 -------QQLLIECPSIYELLASSTYHWEDTPLLQIWRESLDDNGKKSAIL 460 +++ ++ P+I L ++D W D G A + Sbjct: 272 YNYTWSPEKVFVQTPTINYTLRDYRKFFQDIGFEDGWLMRQDTEGLVEATM 322
>YJ68_YEAST (P47139) Hypothetical 74.1 kDa protein in ACR1-YUH1 intergenic| region Length = 655 Score = 35.4 bits (80), Expect = 0.13 Identities = 21/46 (45%), Positives = 27/46 (58%), Gaps = 1/46 (2%) Frame = +2 Query: 20 FGYDFRQSNRLSETLDKFSKKLESVYTASGGKK-INLITHSMGGLL 154 FGYD+R S L + + KLE +Y KK I +I HSMGGL+ Sbjct: 325 FGYDWRLS--LDISAKHLTTKLEEIYNKQKNKKGIYIIAHSMGGLV 368
>RUVA_HAEI8 (Q4QNM5) Holliday junction ATP-dependent DNA helicase ruvA (EC| 3.6.1.-) Length = 204 Score = 33.5 bits (75), Expect = 0.48 Identities = 23/79 (29%), Positives = 40/79 (50%), Gaps = 5/79 (6%) Frame = +2 Query: 311 ISKWTMQQLLIECPSIYELLASSTYHWEDT-----PLLQIWRESLDDNGKKSAILESYEP 475 + K T ++LL+E ++ + S + E T P ++ ES D + I Y+P Sbjct: 116 VGKKTAERLLVELKGKFKGIKQSDFFVESTHIPLSPSIESHSESSSDEAISALIALGYKP 175 Query: 476 DEAIKMIQKALSKHEIISD 532 EA KM+ K ++K E+ S+ Sbjct: 176 AEAEKMV-KRVAKPELTSE 193
>RUVA_HAEIN (P44632) Holliday junction ATP-dependent DNA helicase ruvA (EC| 3.6.1.-) Length = 204 Score = 32.7 bits (73), Expect = 0.81 Identities = 23/79 (29%), Positives = 40/79 (50%), Gaps = 5/79 (6%) Frame = +2 Query: 311 ISKWTMQQLLIECPSIYELLASSTYHWEDT-----PLLQIWRESLDDNGKKSAILESYEP 475 + K T ++LL+E ++ + S + E T P ++ ES D + I Y+P Sbjct: 116 VGKKTAERLLVELKGKFKGVKQSDFFVESTHIPLSPSIESHSESSSDEAISALIALGYKP 175 Query: 476 DEAIKMIQKALSKHEIISD 532 EA KM+ K ++K E+ S+ Sbjct: 176 VEAEKMV-KRVAKPELTSE 193
>LIP_VIBCH (P15493) Lipase precursor (EC 3.1.1.3) (Triacylglycerol lipase)| Length = 312 Score = 32.3 bits (72), Expect = 1.1 Identities = 14/36 (38%), Positives = 23/36 (63%) Frame = +2 Query: 80 KLESVYTASGGKKINLITHSMGGLLVKCFMSLHGDV 187 ++ES+ +G KK+NLI HS GG ++ S+ D+ Sbjct: 91 QVESLLAVTGAKKVNLIGHSHGGPTIRYVASVRPDL 126
>PDAT_YEAST (P40345) Phospholipid:diacylglycerol acyltransferase (EC 2.3.1.158)| (PDAT) Length = 661 Score = 32.0 bits (71), Expect = 1.4 Identities = 28/97 (28%), Positives = 48/97 (49%), Gaps = 14/97 (14%) Frame = +2 Query: 26 YDFRQSNRLSETLDKFSKKLES---VYTASGGKKINLITHSMGGLLVKCFMS-------L 175 YD+R + E D++ KL+ ++ G+K+ LI HSMG ++ FM L Sbjct: 284 YDWRLAYLDLERRDRYFTKLKEQIELFHQLSGEKVCLIGHSMGSQIIFYFMKWVEAEGPL 343 Query: 176 HGD----VFEKYVKSWIAIAAPFQGAPGYINSALLNG 274 +G+ +++ S+I A GAP + AL++G Sbjct: 344 YGNGGRGWVNEHIDSFINAAGTLLGAPKAV-PALISG 379
>BIOD_CHLPN (Q9Z6L7) Dethiobiotin synthetase (EC 6.3.3.3) (Dethiobiotin| synthase) (DTB synthetase) (DTBS) Length = 219 Score = 32.0 bits (71), Expect = 1.4 Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 1/41 (2%) Frame = +2 Query: 122 NLITHSMGGLLVKCFMS-LHGDVFEKYVKSWIAIAAPFQGA 241 NLI + GG L C L GDVF + SWI ++ + G+ Sbjct: 92 NLIIETSGGFLSPCTSKRLQGDVFSSWSCSWILVSQAYLGS 132
>PHBC_RHIET (Q52728) Poly-beta-hydroxybutyrate polymerase (EC 2.3.1.-)| (Poly(3-hydroxybutyrate) polymerase) (PHB polymerase) (PHB synthase) (Poly(3-hydroxyalkanoate) polymerase) (PHA polymerase) (PHA synthase) (Polyhydroxyalkanoic acid synthase) Length = 636 Score = 31.6 bits (70), Expect = 1.8 Identities = 13/42 (30%), Positives = 26/42 (61%) Frame = +2 Query: 83 LESVYTASGGKKINLITHSMGGLLVKCFMSLHGDVFEKYVKS 208 LE++ A+G K++N + + +GG L+ ++LH K +K+ Sbjct: 355 LETIEKATGEKEVNAVGYCVGGTLLAATLALHAKEKNKRIKT 396
>RPOB_FRATT (Q5NID2) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP| beta subunit) (Transcriptase beta chain) (RNA polymerase beta subunit) Length = 1358 Score = 31.6 bits (70), Expect = 1.8 Identities = 28/104 (26%), Positives = 40/104 (38%), Gaps = 1/104 (0%) Frame = +2 Query: 2 GKTLFGFGYDFRQSNRLSETLDKFSKKLESVYTASGGKKINLITHSMGGLLVKCFMSLHG 181 G +G G ++ + + K LE VY + G KK+NL + +L C Sbjct: 1132 GLASYGLGKKIEKTLEKTRKAAELRKTLEEVYNSVGDKKVNLEALNDEEILTLC------ 1185 Query: 182 DVFEKYVKSWIAIAAP-FQGAPGYINSALLNGMSFVEGWQSKFF 310 +K + IA P F GA +LL F Q K F Sbjct: 1186 ----DNLKGGVPIATPVFDGAKEEDIKSLLKIGGFATNGQMKLF 1225
>POLG_PSBMV (P29152) Genome polyprotein [Contains: P1 proteinase (N-terminal| protein); Helper component proteinase (EC 3.4.22.45) (HC-pro); Protein P3; 6 kDa protein 1 (6K1); Cytoplasmic inclusion protein (EC 3.6.1.-) (CI); 6 kDa protein 2 (6K2); Viral ge Length = 3206 Score = 31.2 bits (69), Expect = 2.4 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = +3 Query: 363 SCWLAQPTTGKIHHCYRSGERA*MTMARKVPYWSRMNQM 479 S WL++PT+ +I H Y G A + +++P + QM Sbjct: 458 SNWLSKPTSEQIEHIYERGNLAIQDLNKRIPSAHHVTQM 496
>LIP_PSES5 (P25275) Lipase precursor (EC 3.1.1.3) (Triacylglycerol lipase)| Length = 364 Score = 31.2 bits (69), Expect = 2.4 Identities = 17/53 (32%), Positives = 29/53 (54%) Frame = +2 Query: 83 LESVYTASGGKKINLITHSMGGLLVKCFMSLHGDVFEKYVKSWIAIAAPFQGA 241 +++V A+G K+NL+ HS GGL + ++ D+ V S I P +G+ Sbjct: 113 VKTVLAATGATKVNLVGHSQGGLTSRYVAAVAPDL----VASVTTIGTPHRGS 161
>LCAT_RAT (P18424) Phosphatidylcholine-sterol acyltransferase precursor (EC| 2.3.1.43) (Lecithin-cholesterol acyltransferase) (Phospholipid-cholesterol acyltransferase) Length = 440 Score = 30.8 bits (68), Expect = 3.1 Identities = 23/74 (31%), Positives = 40/74 (54%), Gaps = 2/74 (2%) Frame = +2 Query: 26 YDFRQSNRLS-ETLDKFSKKLESVYTASGGKKINLITHSMGGLLVKCFMSLHGDVF-EKY 199 YD+R + R E K + +E +Y A G K + LI HS+G L V F+ + + + Sbjct: 168 YDWRLAPRQQDEYYQKLAGLVEEMYAAYG-KPVFLIGHSLGCLHVLHFLLRQPQSWKDHF 226 Query: 200 VKSWIAIAAPFQGA 241 + +I++ AP+ G+ Sbjct: 227 IDGFISLGAPWGGS 240
>PDAT_SCHPO (O94680) Phospholipid:diacylglycerol acyltransferase (EC 2.3.1.158)| (PDAT) (Pombe LRO1 homolog 1) Length = 623 Score = 30.8 bits (68), Expect = 3.1 Identities = 29/95 (30%), Positives = 46/95 (48%), Gaps = 12/95 (12%) Frame = +2 Query: 26 YDFRQSNRLSETLDKFSKKLESVYTASG---GKKINLITHSMGGLLVKCFMSL-----HG 181 YD+R S E DK+ KL+ S KK+ LI+HSMG + F +G Sbjct: 253 YDWRLSYANLEERDKYFSKLKMFIEYSNIVHKKKVVLISHSMGSQVTYYFFKWVEAEGYG 312 Query: 182 D----VFEKYVKSWIAIAAPFQGAPGYINSALLNG 274 + +++++I I+ GAP + +ALL+G Sbjct: 313 NGGPTWVNDHIEAFINISGSLIGAPKTV-AALLSG 346
>LIP_BURCE (P22088) Lipase precursor (EC 3.1.1.3) (Triacylglycerol lipase)| Length = 364 Score = 30.8 bits (68), Expect = 3.1 Identities = 17/53 (32%), Positives = 29/53 (54%) Frame = +2 Query: 83 LESVYTASGGKKINLITHSMGGLLVKCFMSLHGDVFEKYVKSWIAIAAPFQGA 241 +++V A+G K+NL+ HS GGL + ++ D+ V S I P +G+ Sbjct: 113 VKTVLAATGATKVNLVGHSQGGLSSRYVAAVAPDL----VASVTTIGTPHRGS 161
>RHAL_RHILO (Q98B65) Probable sugar isomerase mlr5709 (EC 5.3.1.-)| Length = 430 Score = 30.4 bits (67), Expect = 4.0 Identities = 24/84 (28%), Positives = 38/84 (45%), Gaps = 1/84 (1%) Frame = +2 Query: 14 FGFGYDFRQSNRLSETLD-KFSKKLESVYTASGGKKINLITHSMGGLLVKCFMSLHGDVF 190 FG G+D SN S+ D K S K S+ A G + + H++ +C + + + Sbjct: 119 FGLGFDAMNSNTFSDARDQKLSYKFGSLSHADAGTRRQAVEHNL-----EC-IEIGKTLG 172 Query: 191 EKYVKSWIAIAAPFQGAPGYINSA 262 K + WI + F PG +N A Sbjct: 173 SKALTVWIGDGSNF---PGQVNFA 193
>V2A_AMVLE (P03593) RNA-directed RNA polymerase 2A (EC 2.7.7.48) (protein 2A)| Length = 790 Score = 30.4 bits (67), Expect = 4.0 Identities = 17/53 (32%), Positives = 30/53 (56%) Frame = +2 Query: 74 SKKLESVYTASGGKKINLITHSMGGLLVKCFMSLHGDVFEKYVKSWIAIAAPF 232 SK L ++ T SGGK + I + + L+ ++ D+F+++ +SWI I F Sbjct: 662 SKFLITMPTTSGGKVVLPIPNPLKLLIRLGSKKVNADIFDEWYQSWIDIIGGF 714
>LCAT_MOUSE (P16301) Phosphatidylcholine-sterol acyltransferase precursor (EC| 2.3.1.43) (Lecithin-cholesterol acyltransferase) (Phospholipid-cholesterol acyltransferase) Length = 438 Score = 30.0 bits (66), Expect = 5.3 Identities = 22/74 (29%), Positives = 41/74 (55%), Gaps = 2/74 (2%) Frame = +2 Query: 26 YDFRQS-NRLSETLDKFSKKLESVYTASGGKKINLITHSMGGLLVKCFMSLHGDVF-EKY 199 YD+R + ++ E K + +E +Y A G K + LI HS+G L V F+ + + + Sbjct: 168 YDWRLAPHQQDEYYKKLAGLVEEMYAAYG-KPVFLIGHSLGCLHVLHFLLRQPQSWKDHF 226 Query: 200 VKSWIAIAAPFQGA 241 + +I++ AP+ G+ Sbjct: 227 IDGFISLGAPWGGS 240
>LIP_BURGL (Q05489) Lipase precursor (EC 3.1.1.3) (Triacylglycerol lipase)| Length = 358 Score = 30.0 bits (66), Expect = 5.3 Identities = 18/53 (33%), Positives = 27/53 (50%) Frame = +2 Query: 83 LESVYTASGGKKINLITHSMGGLLVKCFMSLHGDVFEKYVKSWIAIAAPFQGA 241 ++ V A+G K+NLI HS GGL + + V + V S I P +G+ Sbjct: 108 VKQVLAATGATKVNLIGHSQGGLTSRYVAA----VAPQLVASVTTIGTPHRGS 156
>TGL2_YEAST (P54857) Lipase 2 (EC 3.1.1.3) (Triacylglycerol lipase)| Length = 326 Score = 29.6 bits (65), Expect = 6.9 Identities = 19/45 (42%), Positives = 23/45 (51%) Frame = +2 Query: 17 GFGYDFRQSNRLSETLDKFSKKLESVYTASGGKKINLITHSMGGL 151 GFG ++ L L K KK+ES +NLI HSMGGL Sbjct: 107 GFGSIEERAMALDAQLQKEVKKIES---KDKRHSLNLIAHSMGGL 148
>LIP_STAEQ (Q5HKP6) Lipase precursor (EC 3.1.1.3) (Glycerol ester hydrolase)| Length = 688 Score = 29.6 bits (65), Expect = 6.9 Identities = 23/63 (36%), Positives = 34/63 (53%), Gaps = 5/63 (7%) Frame = +2 Query: 65 DKFSKKLESVYTA-SGGKKINLITHSMGGLLVKCFMSL--HGDVFE-KYVKSWIAIAAP- 229 +++ K E VY G+KI+L+ HSMGG ++ L HG+ E +Y K +P Sbjct: 393 ERYGKTYEGVYKDWKPGQKIHLVGHSMGGQTIRQLEELLRHGNPEEVEYQKQHGGEISPL 452 Query: 230 FQG 238 FQG Sbjct: 453 FQG 455
>ACKA_STRA5 (Q8E231) Acetate kinase (EC 2.7.2.1) (Acetokinase)| Length = 397 Score = 29.6 bits (65), Expect = 6.9 Identities = 14/37 (37%), Positives = 23/37 (62%) Frame = +2 Query: 434 DNGKKSAILESYEPDEAIKMIQKALSKHEIISDGNHI 544 D+ K IL+ + +A+K++ + L+KH II D N I Sbjct: 47 DDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEI 83
>ACKA_STRA3 (Q8E7I7) Acetate kinase (EC 2.7.2.1) (Acetokinase)| Length = 397 Score = 29.6 bits (65), Expect = 6.9 Identities = 14/37 (37%), Positives = 23/37 (62%) Frame = +2 Query: 434 DNGKKSAILESYEPDEAIKMIQKALSKHEIISDGNHI 544 D+ K IL+ + +A+K++ + L+KH II D N I Sbjct: 47 DDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEI 83
>RGL1_MOUSE (Q60695) Ral guanine nucleotide dissociation stimulator-like 1| (RalGDS-like 1) Length = 768 Score = 29.6 bits (65), Expect = 6.9 Identities = 18/47 (38%), Positives = 29/47 (61%) Frame = +2 Query: 392 EDTPLLQIWRESLDDNGKKSAILESYEPDEAIKMIQKALSKHEIISD 532 EDT +++I E + + KS +L S + A+ IQ+A+SKH + SD Sbjct: 647 EDTCIIRISVEDNNGHMYKSIMLTSQDKTPAV--IQRAMSKHNLESD 691
>YEEJ_ECOLI (P76347) Hypothetical protein yeeJ| Length = 2358 Score = 29.6 bits (65), Expect = 6.9 Identities = 11/35 (31%), Positives = 21/35 (60%) Frame = +2 Query: 434 DNGKKSAILESYEPDEAIKMIQKALSKHEIISDGN 538 +NG K ++ S+ D + + SK+E+++DGN Sbjct: 822 ENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN 856
>YEEJ_ECO57 (Q8X8V7) Hypothetical protein yeeJ| Length = 2660 Score = 29.6 bits (65), Expect = 6.9 Identities = 11/35 (31%), Positives = 21/35 (60%) Frame = +2 Query: 434 DNGKKSAILESYEPDEAIKMIQKALSKHEIISDGN 538 +NG K ++ S+ D + + SK+E+++DGN Sbjct: 822 ENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN 856
>RGL1_HUMAN (Q9NZL6) Ral guanine nucleotide dissociation stimulator-like 1| (RalGDS-like 1) Length = 768 Score = 29.3 bits (64), Expect = 9.0 Identities = 18/47 (38%), Positives = 28/47 (59%) Frame = +2 Query: 392 EDTPLLQIWRESLDDNGKKSAILESYEPDEAIKMIQKALSKHEIISD 532 EDT +++I E + N KS +L S + A+ IQ+A+ KH + SD Sbjct: 647 EDTCIIRISVEDNNGNMYKSIMLTSQDKTPAV--IQRAMLKHNLDSD 691 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 90,159,905 Number of Sequences: 219361 Number of extensions: 1904913 Number of successful extensions: 5010 Number of sequences better than 10.0: 26 Number of HSP's better than 10.0 without gapping: 4902 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5007 length of database: 80,573,946 effective HSP length: 106 effective length of database: 57,321,680 effective search space used: 5216272880 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
