ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | basd18g01 |
|---|---|
| Clone Library Name | barley_pub |
>DHB4_HUMAN (P51659) Peroxisomal multifunctional enzyme type 2 (MFE-2)| (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA dehydratase (EC 4.2.1.107) (3-alpha,7-alpha,12-alpha-trihydroxy-5 Length = 735 Score = 142 bits (357), Expect = 1e-33 Identities = 78/214 (36%), Positives = 117/214 (54%) Frame = +2 Query: 62 ALAYKFPEVSFAYDERDVALYALGVGACGADAVDDKELHLVHHRDGQRHIKALPTFVSLF 241 A+ K P S+AY E + +YALGVGA D K+L ++ +G LPTF + Sbjct: 326 AIGQKLPPFSYAYTELEAIMYALGVGA---SIKDPKDLKFIY--EGSSDFSCLPTFGVII 380 Query: 242 PNKNSNGRGIVNVPGIHFDASLLLHGQQYIEIYKPIPSCASVVNKVKVAGLHDKGKATIL 421 K+ G G+ +PG+ + + +LHG+QY+E+YKP+P + + VA + DKG ++ Sbjct: 381 GQKSMMGGGLAEIPGLSINFAKVLHGEQYLELYKPLPRAGKLKCEAVVADVLDKGSGVVI 440 Query: 422 EIETTTSLKDSGEVLCMNRSTIFLRGAGGFSDSSRPYSYTTYPANQISRISIPNSAPSAV 601 ++ + E++C N+ ++FL G+GGF ++IPN P AV Sbjct: 441 IMDVYS--YSEKELICHNQFSLFLVGSGGFGGKRTSDKVKV-------AVAIPNRPPDAV 491 Query: 602 YEDQTQQSQALLYRLSGDYNPLHSDPMVAQVAGF 703 D T +QA LYRLSGD+NPLH DP A +AGF Sbjct: 492 LTDTTSLNQAALYRLSGDWNPLHIDPNFASLAGF 525
>DHB4_MOUSE (P51660) Peroxisomal multifunctional enzyme type 2 (MFE-2)| (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA dehydratase (EC 4.2.1.107) (3-alpha,7-alpha,12-alpha-trihydroxy-5 Length = 734 Score = 141 bits (356), Expect = 2e-33 Identities = 80/227 (35%), Positives = 123/227 (54%) Frame = +2 Query: 23 TNGAPAASVDPDVALAYKFPEVSFAYDERDVALYALGVGACGADAVDDKELHLVHHRDGQ 202 ++ APAA+ A+ +K P S +Y E +YALGVGA + K+L V+ +G Sbjct: 312 SHAAPAATSGFVGAVGHKLPSFSSSYTELQSIMYALGVGA---SVKNPKDLKFVY--EGS 366 Query: 203 RHIKALPTFVSLFPNKNSNGRGIVNVPGIHFDASLLLHGQQYIEIYKPIPSCASVVNKVK 382 LPTF + K+ G+ VPG+ F+ + LHG+QY+E+YKP+P + + Sbjct: 367 ADFSCLPTFGVIVAQKSMMNGGLAEVPGLSFNFAKALHGEQYLELYKPLPRSGELKCEAV 426 Query: 383 VAGLHDKGKATILEIETTTSLKDSGEVLCMNRSTIFLRGAGGFSDSSRPYSYTTYPANQI 562 +A + DKG ++ ++ + E++C N+ ++F+ G+GGF Sbjct: 427 IADILDKGSGVVIVMDVYSY--SGKELICYNQFSVFVVGSGGFGGKRTSEKLK------- 477 Query: 563 SRISIPNSAPSAVYEDQTQQSQALLYRLSGDYNPLHSDPMVAQVAGF 703 + +++PN P AV D T +QA LYRLSGD+NPLH DP A VAGF Sbjct: 478 AAVAVPNRPPDAVLRDATSLNQAALYRLSGDWNPLHIDPDFASVAGF 524
>DHB4_RAT (P97852) Peroxisomal multifunctional enzyme type 2 (MFE-2)| (D-bifunctional protein) (DBP) (17-beta-hydroxysteroid dehydrogenase 4) (17-beta-HSD 4) [Includes: D-3-hydroxyacyl-CoA dehydratase (EC 4.2.1.107) (3-alpha,7-alpha,12-alpha-trihydroxy-5-b Length = 734 Score = 131 bits (330), Expect = 2e-30 Identities = 72/213 (33%), Positives = 114/213 (53%) Frame = +2 Query: 65 LAYKFPEVSFAYDERDVALYALGVGACGADAVDDKELHLVHHRDGQRHIKALPTFVSLFP 244 + +K P S +Y E +YALGVGA + K+L V+ +G LPTF + Sbjct: 326 VGHKLPSFSSSYTELQCIMYALGVGA---SVKNPKDLKFVY--EGSADFSCLPTFGVIVA 380 Query: 245 NKNSNGRGIVNVPGIHFDASLLLHGQQYIEIYKPIPSCASVVNKVKVAGLHDKGKATILE 424 K+ G+ VPG+ + + +LHG+QY+E+YKP+P + + +A + DKG ++ Sbjct: 381 QKSLMSGGLAEVPGLSINFAKVLHGEQYLELYKPLPRSGELKCEAVIADILDKGSGIVIV 440 Query: 425 IETTTSLKDSGEVLCMNRSTIFLRGAGGFSDSSRPYSYTTYPANQISRISIPNSAPSAVY 604 ++ + E++C N+ ++F+ G+GGF + +++P+ P AV Sbjct: 441 MDVYS--YSGKELICYNQFSVFVVGSGGFGGKRTSEKLK-------AAVAVPSRPPDAVL 491 Query: 605 EDQTQQSQALLYRLSGDYNPLHSDPMVAQVAGF 703 D T +QA LYRLSGD NPLH DP A +AGF Sbjct: 492 RDTTSLNQAALYRLSGDSNPLHIDPSFASIAGF 524
>FOX2_NEUCR (Q01373) Peroxisomal hydratase-dehydrogenase-epimerase (HDE)| (Multifunctional beta-oxidation protein) (MFP) [Includes: 2-enoyl-CoA hydratase (EC 4.2.1.-); D-3-hydroxyacyl CoA dehydrogenase (EC 1.1.1.-)] Length = 894 Score = 128 bits (322), Expect = 1e-29 Identities = 85/231 (36%), Positives = 119/231 (51%), Gaps = 6/231 (2%) Frame = +2 Query: 29 GAPAASVDPDVALAYKFPEVS-----FAYDERDVALYALGVGACGADAVDDKELHLVHHR 193 G A+ + +A KF V + + ERDV LY LG+GA K + + Sbjct: 602 GKSGAAENEHLAAIKKFTGVEGKGTEYTFTERDVCLYNLGIGA--------KRTDIKYIF 653 Query: 194 DGQRHIKALPTFVSLFPNKNSNGRGIVNVPGIHFDASLLLHGQQYIEIYK-PIPSCASVV 370 +G + +PTF + P N+ + +F +LLHG+QY+E+ K PIP+ +V Sbjct: 654 EGNEDFEVVPTF-GVIPPFNTEMPFSFDDIVPNFSPMMLLHGEQYLEVRKYPIPTSGRLV 712 Query: 371 NKVKVAGLHDKGKATILEIETTTSLKDSGEVLCMNRSTIFLRGAGGFSDSSRPYSYTTYP 550 +K K+ + DKG A I++ TT ++GE L N T+FLRG GGF +P Sbjct: 713 SKGKLLEVVDKGSAAIVKQGITTFNAETGEELFYNEMTVFLRGCGGFGGQKKPADRGAST 772 Query: 551 ANQISRISIPNSAPSAVYEDQTQQSQALLYRLSGDYNPLHSDPMVAQVAGF 703 A P +P AV E QT + QA +YRLSGDYNPLH DP A+V GF Sbjct: 773 AAN----KPPARSPDAVVEVQTTEEQAAIYRLSGDYNPLHVDPAFAKVGGF 819
>FOX2_CANTR (P22414) Peroxisomal hydratase-dehydrogenase-epimerase (HDE)| (Multifunctional beta-oxidation protein) (MFP) [Includes: 2-enoyl-CoA hydratase (EC 4.2.1.-); D-3-hydroxyacyl CoA dehydrogenase (EC 1.1.1.-)] Length = 906 Score = 78.2 bits (191), Expect = 2e-14 Identities = 57/203 (28%), Positives = 91/203 (44%), Gaps = 1/203 (0%) Frame = +2 Query: 98 YDERDVALYALGVGACGADAVDDKELHLVHHRDGQRHIKALPTFVSLFPNKNSNGRGIVN 277 +D+RDV LY + +GA K+L V+ D + +PTF L + + Sbjct: 635 FDDRDVILYNIALGAT------TKQLKYVYENDSD--FQVIPTFGHLITFNSGKSQNSFA 686 Query: 278 VPGIHFDASLLLHGQQYIEIYK-PIPSCASVVNKVKVAGLHDKGKATILEIETTTSLKDS 454 +F+ LLLHG+ Y++++ P P+ + + KG ++ + + S Sbjct: 687 KLLRNFNPMLLLHGEHYLKVHSWPPPTEGEIKTTFEPIATTPKGTNVVIVHGSKSVDNKS 746 Query: 455 GEVLCMNRSTIFLRGAGGFSDSSRPYSYTTYPANQISRISIPNSAPSAVYEDQTQQSQAL 634 GE++ N +T F+R + Y PA ++ P AP + + A Sbjct: 747 GELIYSNEATYFIRNCQADNKV-----YADRPAFATNQFLAPKRAPDYQVDVPVSEDLAA 801 Query: 635 LYRLSGDYNPLHSDPMVAQVAGF 703 LYRLSGD NPLH DP A+ A F Sbjct: 802 LYRLSGDRNPLHIDPNFAKGAKF 824
>FOX2_YEAST (Q02207) Peroxisomal hydratase-dehydrogenase-epimerase (HDE)| (Multifunctional beta-oxidation protein) (MFP) [Includes: 2-enoyl-CoA hydratase (EC 4.2.1.-); D-3-hydroxyacyl CoA dehydrogenase (EC 1.1.1.-)] Length = 900 Score = 73.2 bits (178), Expect = 7e-13 Identities = 60/207 (28%), Positives = 96/207 (46%), Gaps = 3/207 (1%) Frame = +2 Query: 92 FAYDERDVALYALGVGACGADAVDDKELHLVHHRDGQRHIKALPTFVSLFPNKNSNGRGI 271 F Y +D LY LG+G KEL + D + LPTF ++ P + Sbjct: 624 FKYTTKDCILYNLGLGCTS------KELKYTYENDPD--FQVLPTF-AVIPFMQATATLA 674 Query: 272 VNVPGIHFDASLLLHGQQYIEIYKP-IPSCASVVNKVKVAGLHDK-GKATILEIETTTSL 445 ++ +F+ ++LLHG+QY ++ P +PS ++ K + DK GKA ++ T Sbjct: 675 MDNLVDNFNYAMLLHGEQYFKLCTPTMPSNGTLKTLAKPLQVLDKNGKAALVVGGFETYD 734 Query: 446 KDSGEVLCMNRSTIFLRGAGGFSDSSRPYSYTTYPANQISRISIPNS-APSAVYEDQTQQ 622 + +++ N + F+RGA + + +P+ P E T + Sbjct: 735 IKTKKLIAYNEGSFFIRGA--HVPPEKEVRDGKRAKFAVQNFEVPHGKVPDFEAEISTNK 792 Query: 623 SQALLYRLSGDYNPLHSDPMVAQVAGF 703 QA LYRLSGD+NPLH DP +A+ F Sbjct: 793 DQAALYRLSGDFNPLHIDPTLAKAVKF 819
>FOG2_HUMAN (Q8WW38) Zinc finger protein ZFPM2 (Zinc finger protein multitype| 2) (Friend of GATA protein 2) (FOG-2) (hFOG-2) Length = 1151 Score = 33.5 bits (75), Expect = 0.65 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = -2 Query: 598 CRRCGIWNRNARNLVGRISRIRVWPGRVRKTSSTTQENSTPVH 470 C+ CGIW R+ RNL + + GR R+ + ++EN H Sbjct: 252 CKSCGIWYRSERNLQAHL--MYYCSGRQREAAPVSEENEDSAH 292
>FOG2_MOUSE (Q8CCH7) Zinc finger protein ZFPM2 (Zinc finger protein multitype| 2) (Friend of GATA protein 2) (Friend of GATA-2) (FOG-2) (mFOG-2) Length = 1151 Score = 32.3 bits (72), Expect = 1.4 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = -2 Query: 598 CRRCGIWNRNARNLVGRISRIRVWPGRVRKTSSTTQENSTPVH 470 C+ CGIW R+ RNL + + GR R+ + ++EN H Sbjct: 252 CKSCGIWYRSERNLQAHL--MYYCSGRQREAAPVSEENEDNSH 292
>HEM3_STAES (Q8CNY8) Porphobilinogen deaminase (EC 2.5.1.61) (PBG)| (Hydroxymethylbilane synthase) (HMBS) (Pre-uroporphyrinogen synthase) Length = 308 Score = 31.2 bits (69), Expect = 3.2 Identities = 21/51 (41%), Positives = 29/51 (56%), Gaps = 5/51 (9%) Frame = +2 Query: 98 YDERDVALYALGVGACGADA-VDDKE----LHLVHHRDGQRHIKALPTFVS 235 Y +RD+ L A+G GA G + DDKE L VH+ D + + A TF+S Sbjct: 185 YLDRDILLPAIGQGALGIECRSDDKELLDLLSKVHNHDVAQCVTAERTFLS 235
>HEM3_STAEQ (Q5HNN3) Porphobilinogen deaminase (EC 2.5.1.61) (PBG)| (Hydroxymethylbilane synthase) (HMBS) (Pre-uroporphyrinogen synthase) Length = 308 Score = 31.2 bits (69), Expect = 3.2 Identities = 21/51 (41%), Positives = 29/51 (56%), Gaps = 5/51 (9%) Frame = +2 Query: 98 YDERDVALYALGVGACGADA-VDDKE----LHLVHHRDGQRHIKALPTFVS 235 Y +RD+ L A+G GA G + DDKE L VH+ D + + A TF+S Sbjct: 185 YLDRDILLPAIGQGALGIECRSDDKELLDLLSKVHNHDVAQCVTAERTFLS 235
>CRYS_DICDI (P21837) Crystal protein precursor| Length = 550 Score = 30.8 bits (68), Expect = 4.2 Identities = 17/55 (30%), Positives = 29/55 (52%) Frame = -2 Query: 526 PGRVRKTSSTTQENSTPVHT*HFTRILEAGRSFDLENSCFPFVVQTSYLNLVYNT 362 PGR S + Q N +P++ + ++L G SF+ +C V + L +V+NT Sbjct: 403 PGRYH-VSKSAQANESPIYHYQYKQVLSGGHSFE---ACEGLVCHGTELPMVFNT 453
>ZP2_HUMAN (Q05996) Zona pellucida sperm-binding protein 2 precursor (Zona| pellucida glycoprotein ZP2) (Zona pellucida protein A) Length = 745 Score = 30.4 bits (67), Expect = 5.5 Identities = 25/74 (33%), Positives = 34/74 (45%), Gaps = 3/74 (4%) Frame = +2 Query: 407 KATILEIETTTSLKDSGEVLCMNRSTIF---LRGAGGFSDSSRPYSYTTYPANQISRISI 577 K T L T S+ E LC + +I L GF D YSY T PA + + + Sbjct: 340 KLTFLLRPETVSMVIYPECLCESPVSIVTGELCTQDGFMDVE-VYSYQTQPALDLGTLRV 398 Query: 578 PNSAPSAVYEDQTQ 619 NS+ V+E Q+Q Sbjct: 399 GNSSCQPVFEAQSQ 412
>TOXC_COCCA (Q92215) Putative fatty acid synthase subunit TOXC [Includes:| 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.61); Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9); [Acyl-carrier-protein] acetyltransferase (EC 2.3.1.3 Length = 2080 Score = 30.0 bits (66), Expect = 7.2 Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 3/58 (5%) Frame = +2 Query: 536 YTTYPANQIS---RISIPNSAPSAVYEDQTQQSQALLYRLSGDYNPLHSDPMVAQVAG 700 Y +Y N + +S+PN+ S V S R SGD NP+H A+ AG Sbjct: 1537 YGSYSQNSVDFEDPVSVPNNGESLVI--CAPSSNEAYARTSGDLNPIHVSRTFAEYAG 1592
>FAS1_YEAST (P07149) Fatty acid synthase beta subunit (EC 2.3.1.86) [Includes:| 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase (EC 4.2.1.61); Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9); [Acyl-carrier-protein] acetyltransferase (EC 2. Length = 2051 Score = 30.0 bits (66), Expect = 7.2 Identities = 12/35 (34%), Positives = 20/35 (57%) Frame = +2 Query: 566 RISIPNSAPSAVYEDQTQQSQALLYRLSGDYNPLH 670 ++++ N P AV + T + R+SGD NP+H Sbjct: 1530 KVNLENPIPIAVLDSYTPSTNEPYARVSGDLNPIH 1564
>HEM3_BACSK (Q5WEP5) Porphobilinogen deaminase (EC 2.5.1.61) (PBG)| (Hydroxymethylbilane synthase) (HMBS) (Pre-uroporphyrinogen synthase) Length = 311 Score = 30.0 bits (66), Expect = 7.2 Identities = 37/141 (26%), Positives = 65/141 (46%), Gaps = 6/141 (4%) Frame = +2 Query: 83 EVSFAYDERDVALYALGVGACGADA-VDDKE----LHLVHHRDGQRHIKALPTFVSLFPN 247 +V Y ++DV L A+G GA G + VDD E L +HH + + + A F+ Sbjct: 180 DVITEYLDKDVCLPAIGQGALGLECRVDDVETTELLAKLHHEETGKAVLAERAFL----- 234 Query: 248 KNSNGRGIVNVPGIHFDASLLLHGQQYIEIYKPIPSCASVVNKVKVAGLHDK-GKATILE 424 K NG V + G A++L G ++ P +++ + K + D+ GK E Sbjct: 235 KEMNGGCQVPIGGY---ATVLEDGAVFLTGLVGSPDGKTILKEGKCGMVADELGK----E 287 Query: 425 IETTTSLKDSGEVLCMNRSTI 487 + + + + +G +L RS + Sbjct: 288 VSSLLTAQGAGAILEKVRSEL 308
>MRE11_PYRAB (Q9UZC9) DNA double-strand break repair protein mre11| Length = 423 Score = 29.6 bits (65), Expect = 9.4 Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 3/64 (4%) Frame = +2 Query: 464 LCMNRSTIFLRGAGGFSDSSRPYSYTTYPANQISRISIPNSAPSAVYE---DQTQQSQAL 634 +C++ F+ AG +SSRP T A +I +I N+ P E D+TQ+ ++ Sbjct: 45 ICVDEKVDFIVIAGDLFNSSRPSPGTIKTAVKILQIPRDNNIPVFAIEGNHDRTQRGPSI 104 Query: 635 LYRL 646 L+ L Sbjct: 105 LHLL 108
>FOLD_SALTY (P58688) FolD bifunctional protein [Includes:| Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)] Length = 287 Score = 29.6 bits (65), Expect = 9.4 Identities = 17/52 (32%), Positives = 24/52 (46%) Frame = -3 Query: 510 KPPAPRKKIVLLFIHNTSPESLRLVVVSISRIVAFPLSCKPATLTLFTTLAH 355 +P PR + LL +N L VV+ S IV P+S + TT+ H Sbjct: 138 RPCTPRGIVTLLERYNIDTYGLNAVVIGASNIVGRPMSMELLLAGCTTTVTH 189
>FOLD_SALTI (Q60006) FolD bifunctional protein [Includes:| Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)] Length = 287 Score = 29.6 bits (65), Expect = 9.4 Identities = 17/52 (32%), Positives = 24/52 (46%) Frame = -3 Query: 510 KPPAPRKKIVLLFIHNTSPESLRLVVVSISRIVAFPLSCKPATLTLFTTLAH 355 +P PR + LL +N L VV+ S IV P+S + TT+ H Sbjct: 138 RPCTPRGIVTLLERYNIDTYGLNAVVIGASNIVGRPMSMELLLAGCTTTVTH 189
>YKD3A_YEAST (Q86ZR7) Hypothetical protein YKL033W-A| Length = 236 Score = 29.6 bits (65), Expect = 9.4 Identities = 16/39 (41%), Positives = 18/39 (46%) Frame = +2 Query: 29 GAPAASVDPDVALAYKFPEVSFAYDERDVALYALGVGAC 145 G P V YK P YDER+VAL +L G C Sbjct: 51 GLPGPEAGKRVIEHYKLPITLDEYDERNVALQSLKWGTC 89
>MRE11_PYRHO (O58686) DNA double-strand break repair protein mre11| Length = 413 Score = 29.6 bits (65), Expect = 9.4 Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 3/64 (4%) Frame = +2 Query: 464 LCMNRSTIFLRGAGGFSDSSRPYSYTTYPANQISRISIPNSAPSAVYE---DQTQQSQAL 634 +C+ S F+ AG +SSRP T A ++ +I N+ P E D+TQ+ ++ Sbjct: 35 MCVKESVDFIIIAGDLFNSSRPSPGTIKTAIKLLQIPKENNIPVFAIEGNHDRTQRGPSV 94 Query: 635 LYRL 646 L+ L Sbjct: 95 LHLL 98 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 90,928,713 Number of Sequences: 219361 Number of extensions: 1914001 Number of successful extensions: 5504 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 5353 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5489 length of database: 80,573,946 effective HSP length: 109 effective length of database: 56,663,597 effective search space used: 7082949625 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
