>dbj|D26105.1|BLYHEMH1 Barley mRNA for ferrochelatase
          Length = 1728

 Score =  551 bits (278), Expect = e-154
 Identities = 283/285 (99%)
 Strand = Plus / Plus

                                                                       
Query: 1   atttttgttcaacctcttcgctgatccagatatcattcgactccctaggctgttcaggtt 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 471 atttttgttcaacctcttcgctgatccagatatcattcgactccctaggctgttcaggtt 530

                                                                       
Query: 61  tcttcaaagaccactggccaaacttatatctacttttagagctcctaagagtaaagaggg 120
           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct: 531 tcttcaaagaccactggccaaacttatatctacttttagagctcctaagagtaacgaggg 590

                                                                       
Query: 121 gtatgcctcaattggtggtggatcacctctgcggaaaattaccgacgagcaggcaaatgc 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 591 gtatgcctcaattggtggtggatcacctctgcggaaaattaccgacgagcaggcaaatgc 650

                                                                       
Query: 181 tttgaaggttgcactaaaaagtaagaacttggaagcagatatttatgttggaatgcggna 240
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct: 651 tttgaaggttgcactaaaaagtaagaacttggaagcagatatttatgttggaatgcggta 710

                                                        
Query: 241 ttggtacccattcaccgaagaagccattgatcagattaagaagga 285
           |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 711 ttggtacccattcaccgaagaagccattgatcagattaagaagga 755

>gb|AF020791.1|AF020791 Hordeum vulgare ferrochelatase (hemH) gene, complete cds
          Length = 7694

 Score =  287 bits (145), Expect = 1e-74
 Identities = 145/145 (100%)
 Strand = Plus / Plus

                                                                        
Query: 29   gatatcattcgactccctaggctgttcaggtttcttcaaagaccactggccaaacttata 88
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3449 gatatcattcgactccctaggctgttcaggtttcttcaaagaccactggccaaacttata 3508

                                                                        
Query: 89   tctacttttagagctcctaagagtaaagaggggtatgcctcaattggtggtggatcacct 148
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3509 tctacttttagagctcctaagagtaaagaggggtatgcctcaattggtggtggatcacct 3568

                                     
Query: 149  ctgcggaaaattaccgacgagcagg 173
            |||||||||||||||||||||||||
Sbjct: 3569 ctgcggaaaattaccgacgagcagg 3593



 Score =  202 bits (102), Expect = 4e-49
 Identities = 104/105 (99%)
 Strand = Plus / Plus

                                                                        
Query: 170  caggcaaatgctttgaaggttgcactaaaaagtaagaacttggaagcagatatttatgtt 229
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3783 caggcaaatgctttgaaggttgcactaaaaagtaagaacttggaagcagatatttatgtt 3842

                                                         
Query: 230  ggaatgcggnattggtacccattcaccgaagaagccattgatcag 274
            ||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 3843 ggaatgcggtattggtacccattcaccgaagaagccattgatcag 3887



 Score = 58.0 bits (29), Expect = 2e-05
 Identities = 29/29 (100%)
 Strand = Plus / Plus

                                         
Query: 1    atttttgttcaacctcttcgctgatccag 29
            |||||||||||||||||||||||||||||
Sbjct: 2847 atttttgttcaacctcttcgctgatccag 2875

>gb|AY108431.1| Zea mays PCO068191 mRNA sequence
          Length = 1776

 Score =  274 bits (138), Expect = 1e-70
 Identities = 248/285 (87%)
 Strand = Plus / Plus

                                                                       
Query: 1   atttttgttcaacctcttcgctgatccagatatcattcgactccctaggctgttcaggtt 60
           |||||| |||||||| || |||||||||||||||||||||||||||||||| ||||||||
Sbjct: 444 atttttattcaacctatttgctgatccagatatcattcgactccctaggctcttcaggtt 503

                                                                       
Query: 61  tcttcaaagaccactggccaaacttatatctacttttagagctcctaagagtaaagaggg 120
           ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| ||
Sbjct: 504 tcttcaaagaccactggccaaacttatttctacttttagagctcctaagagtaaagaagg 563

                                                                       
Query: 121 gtatgcctcaattggtggtggatcacctctgcggaaaattaccgacgagcaggcaaatgc 180
           |||||| |||||||||||||| || ||  |  |||||||||| || || ||||| |||||
Sbjct: 564 gtatgcttcaattggtggtgggtcgccgttaaggaaaattactgatgaacaggcgaatgc 623

                                                                       
Query: 181 tttgaaggttgcactaaaaagtaagaacttggaagcagatatttatgttggaatgcggna 240
           ||||||| |||| ||  |||  || || ||| | ||| | || |||||||| |||||| |
Sbjct: 624 tttgaagattgccctggaaaagaaaaaattgaacgcaaacatatatgttgggatgcggta 683

                                                        
Query: 241 ttggtacccattcaccgaagaagccattgatcagattaagaagga 285
           ||||||||| ||||| ||||| ||||||||||| ||||| |||||
Sbjct: 684 ttggtaccctttcacagaagaggccattgatcaaattaaaaagga 728

>dbj|AK068174.1| Oryza sativa (japonica cultivar-group) cDNA clone:J013129M17, full
           insert sequence
          Length = 1963

 Score =  260 bits (131), Expect = 2e-66
 Identities = 241/278 (86%)
 Strand = Plus / Plus

                                                                       
Query: 8   ttcaacctcttcgctgatccagatatcattcgactccctaggctgttcaggtttcttcaa 67
           |||||||| || ||||| ||||||||||| ||  | || |||||||||||||||||||||
Sbjct: 568 ttcaacctgtttgctgacccagatatcatccgtttgccaaggctgttcaggtttcttcaa 627

                                                                       
Query: 68  agaccactggccaaacttatatctacttttagagctcctaagagtaaagaggggtatgcc 127
           |||||| ||||||| ||||| || |||||||||||||| |||||||| |||||||| || 
Sbjct: 628 agaccattggccaagcttatctccacttttagagctccaaagagtaaggaggggtacgct 687

                                                                       
Query: 128 tcaattggtggtggatcacctctgcggaaaattaccgacgagcaggcaaatgctttgaag 187
           ||||| ||||| ||||||||| ||||||||||||| || |||||||||||||||||||||
Sbjct: 688 tcaatcggtggaggatcacctttgcggaaaattactgatgagcaggcaaatgctttgaag 747

                                                                       
Query: 188 gttgcactaaaaagtaagaacttggaagcagatatttatgttggaatgcggnattggtac 247
           || ||||| ||||  ||||| ||| | ||  |||| ||||||||||||||  | ||||||
Sbjct: 748 gtggcactgaaaaagaagaatttgaatgctaatatatatgttggaatgcgatactggtac 807

                                                 
Query: 248 ccattcaccgaagaagccattgatcagattaagaagga 285
           || || || |||||||||||||||||||||||||||||
Sbjct: 808 ccttttacagaagaagccattgatcagattaagaagga 845

>dbj|AP008215.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 9, complete
               sequence
          Length = 22696651

 Score =  149 bits (75), Expect = 6e-33
 Identities = 114/127 (89%)
 Strand = Plus / Plus

                                                                           
Query: 47      aggctgttcaggtttcttcaaagaccactggccaaacttatatctacttttagagctcct 106
               ||||||||||||||||||||||||||| ||||||| ||||| || |||||||||||||| 
Sbjct: 7147184 aggctgttcaggtttcttcaaagaccattggccaagcttatctccacttttagagctcca 7147243

                                                                           
Query: 107     aagagtaaagaggggtatgcctcaattggtggtggatcacctctgcggaaaattaccgac 166
               |||||||| |||||||| || ||||| ||||| ||||||||| ||||||||||||| || 
Sbjct: 7147244 aagagtaaggaggggtacgcttcaatcggtggaggatcacctttgcggaaaattactgat 7147303

                      
Query: 167     gagcagg 173
               |||||||
Sbjct: 7147304 gagcagg 7147310



 Score = 83.8 bits (42), Expect = 3e-13
 Identities = 89/105 (84%)
 Strand = Plus / Plus

                                                                           
Query: 170     caggcaaatgctttgaaggttgcactaaaaagtaagaacttggaagcagatatttatgtt 229
               |||||||||||||||||||| ||||| ||||  ||||| ||| | ||  |||| ||||||
Sbjct: 7147460 caggcaaatgctttgaaggtggcactgaaaaagaagaatttgaatgctaatatatatgtt 7147519

                                                            
Query: 230     ggaatgcggnattggtacccattcaccgaagaagccattgatcag 274
               ||||||||  | |||||||| || || ||||||||||||||||||
Sbjct: 7147520 ggaatgcgatactggtacccttttacagaagaagccattgatcag 7147564

>dbj|AP004756.2| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 9, PAC
             clone:P0592C05
          Length = 150478

 Score =  149 bits (75), Expect = 6e-33
 Identities = 114/127 (89%)
 Strand = Plus / Plus

                                                                         
Query: 47    aggctgttcaggtttcttcaaagaccactggccaaacttatatctacttttagagctcct 106
             ||||||||||||||||||||||||||| ||||||| ||||| || |||||||||||||| 
Sbjct: 99263 aggctgttcaggtttcttcaaagaccattggccaagcttatctccacttttagagctcca 99322

                                                                         
Query: 107   aagagtaaagaggggtatgcctcaattggtggtggatcacctctgcggaaaattaccgac 166
             |||||||| |||||||| || ||||| ||||| ||||||||| ||||||||||||| || 
Sbjct: 99323 aagagtaaggaggggtacgcttcaatcggtggaggatcacctttgcggaaaattactgat 99382

                    
Query: 167   gagcagg 173
             |||||||
Sbjct: 99383 gagcagg 99389



 Score = 83.8 bits (42), Expect = 3e-13
 Identities = 89/105 (84%)
 Strand = Plus / Plus

                                                                         
Query: 170   caggcaaatgctttgaaggttgcactaaaaagtaagaacttggaagcagatatttatgtt 229
             |||||||||||||||||||| ||||| ||||  ||||| ||| | ||  |||| ||||||
Sbjct: 99539 caggcaaatgctttgaaggtggcactgaaaaagaagaatttgaatgctaatatatatgtt 99598

                                                          
Query: 230   ggaatgcggnattggtacccattcaccgaagaagccattgatcag 274
             ||||||||  | |||||||| || || ||||||||||||||||||
Sbjct: 99599 ggaatgcgatactggtacccttttacagaagaagccattgatcag 99643

>gb|AY106755.1| Zea mays PCO095974 mRNA sequence
          Length = 755

 Score =  139 bits (70), Expect = 5e-30
 Identities = 100/110 (90%)
 Strand = Plus / Plus

                                                                       
Query: 5   ttgttcaacctcttcgctgatccagatatcattcgactccctaggctgttcaggtttctt 64
           ||||||||||| || ||||||||||||||||||||||| | |||||| |||||||| || 
Sbjct: 646 ttgttcaacctatttgctgatccagatatcattcgactgcttaggctcttcaggttcctc 705

                                                             
Query: 65  caaagaccactggccaaacttatatctacttttagagctcctaagagtaa 114
           |||||||||||||||||||| ||  |||||||||||||||||||||||||
Sbjct: 706 caaagaccactggccaaactaattcctacttttagagctcctaagagtaa 755

>emb|BX820158.1|CNS0A82Y Arabidopsis thaliana Full-length cDNA Complete sequence from clone
           GSLTLS82ZH07 of Adult vegetative tissue of strain col-0
           of Arabidopsis thaliana (thale cress)
          Length = 1795

 Score = 54.0 bits (27), Expect = 2e-04
 Identities = 50/58 (86%)
 Strand = Plus / Plus

                                                                     
Query: 224 tatgttggaatgcggnattggtacccattcaccgaagaagccattgatcagattaaga 281
           |||||||| |||||| ||||| | |||||||| || ||||||||||| ||||| ||||
Sbjct: 665 tatgttggtatgcggtattggcatccattcactgaggaagccattgaacagataaaga 722

>emb|CT009490.7| M.truncatula DNA sequence from clone MTH2-174P8 on chromosome 3,
             complete sequence
          Length = 107411

 Score = 54.0 bits (27), Expect = 2e-04
 Identities = 41/46 (89%)
 Strand = Plus / Minus

                                                           
Query: 219   atatttatgttggaatgcggnattggtacccattcaccgaagaagc 264
             |||| |||||||| |||||| | |||||||||||||| ||||||||
Sbjct: 48213 atatctatgttgggatgcggtactggtacccattcactgaagaagc 48168

>ref|NM_128592.2| Arabidopsis thaliana ferrochelatase AT2G30390 mRNA, complete cds
          Length = 1859

 Score = 52.0 bits (26), Expect = 0.001
 Identities = 46/53 (86%)
 Strand = Plus / Plus

                                                                
Query: 224 tatgttggaatgcggnattggtacccattcaccgaagaagccattgatcagat 276
           |||||||| |||||| ||||| | |||||||| || ||||||||||| |||||
Sbjct: 685 tatgttggtatgcggtattggcatccattcactgaggaagccattgaacagat 737

>emb|AJ316569.1|NTA316569 Nicotiana tabacum mRNA for ferrochelatase precursor (FeCh gene)
          Length = 2118

 Score = 52.0 bits (26), Expect = 0.001
 Identities = 47/54 (87%)
 Strand = Plus / Plus

                                                                 
Query: 97  tagagctcctaagagtaaagaggggtatgcctcaattggtggtggatcacctct 150
           |||||| |||||||| ||||| || ||||| |||||||||||||| || |||||
Sbjct: 476 tagagcacctaagagcaaagaaggttatgcttcaattggtggtggctctcctct 529

>gb|BT008877.1| Arabidopsis thaliana At2g30390 mRNA, complete cds
          Length = 1539

 Score = 52.0 bits (26), Expect = 0.001
 Identities = 46/53 (86%)
 Strand = Plus / Plus

                                                                
Query: 224 tatgttggaatgcggnattggtacccattcaccgaagaagccattgatcagat 276
           |||||||| |||||| ||||| | |||||||| || ||||||||||| |||||
Sbjct: 580 tatgttggtatgcggtattggcatccattcactgaggaagccattgaacagat 632

>gb|BT000465.1| Arabidopsis thaliana putative ferrochelatase precusor (At2g30390)
           mRNA, complete cds
          Length = 1860

 Score = 52.0 bits (26), Expect = 0.001
 Identities = 46/53 (86%)
 Strand = Plus / Plus

                                                                
Query: 224 tatgttggaatgcggnattggtacccattcaccgaagaagccattgatcagat 276
           |||||||| |||||| ||||| | |||||||| || ||||||||||| |||||
Sbjct: 686 tatgttggtatgcggtattggcatccattcactgaggaagccattgaacagat 738

>emb|Y13156.1|ATFERROCH Arabidopsis thaliana mRNA for ferrochelatase
          Length = 1797

 Score = 52.0 bits (26), Expect = 0.001
 Identities = 46/53 (86%)
 Strand = Plus / Plus

                                                                
Query: 224 tatgttggaatgcggnattggtacccattcaccgaagaagccattgatcagat 276
           |||||||| |||||| ||||| | |||||||| || ||||||||||| |||||
Sbjct: 604 tatgttggtatgcggtattggcatccattcactgaggaagccattgaacagat 656

>gb|AF428371.1|AF428371 Arabidopsis thaliana T9D9.20/T9D9.20 mRNA, complete cds
          Length = 1769

 Score = 52.0 bits (26), Expect = 0.001
 Identities = 46/53 (86%)
 Strand = Plus / Plus

                                                                
Query: 224 tatgttggaatgcggnattggtacccattcaccgaagaagccattgatcagat 276
           |||||||| |||||| ||||| | |||||||| || ||||||||||| |||||
Sbjct: 630 tatgttggtatgcggtattggcatccattcactgaggaagccattgaacagat 682

>gb|AC148755.6| Medicago truncatula clone mth2-24n16, complete sequence
          Length = 119876

 Score = 48.1 bits (24), Expect = 0.015
 Identities = 75/92 (81%)
 Strand = Plus / Plus

                                                                         
Query: 59    tttcttcaaagaccactggccaaacttatatctacttttagagctcctaagagtaaagag 118
             ||||||||||  ||||||||  || || |||||  ||| ||||| || ||||| ||||| 
Sbjct: 84860 tttcttcaaaagccactggctcaatttgtatctgttttaagagcaccaaagagcaaagaa 84919

                                             
Query: 119   gggtatgcctcaattggtggtggatcacctct 150
             || ||||| || |||||||||||||| |||||
Sbjct: 84920 ggctatgcttccattggtggtggatcccctct 84951

>gb|U93215.3| Arabidopsis thaliana chromosome 2 BAC T6B20 genomic sequence, complete
              sequence
          Length = 104861

 Score = 48.1 bits (24), Expect = 0.015
 Identities = 41/47 (87%)
 Strand = Plus / Plus

                                                             
Query: 224    tatgttggaatgcggnattggtacccattcaccgaagaagccattga 270
              |||||||| |||||| ||||| | |||||||| || |||||||||||
Sbjct: 100507 tatgttggtatgcggtattggcatccattcactgaggaagccattga 100553

>gb|AC002338.3| Arabidopsis thaliana chromosome 2 clone T9D9 map m283, complete
             sequence
          Length = 79272

 Score = 48.1 bits (24), Expect = 0.015
 Identities = 41/47 (87%)
 Strand = Plus / Minus

                                                            
Query: 224   tatgttggaatgcggnattggtacccattcaccgaagaagccattga 270
             |||||||| |||||| ||||| | |||||||| || |||||||||||
Sbjct: 68648 tatgttggtatgcggtattggcatccattcactgaggaagccattga 68602

>emb|AJ005802.1|STU5802 Solanum tuberosum, cultivar binje, mRNA for ferrochelatase
          Length = 2051

 Score = 44.1 bits (22), Expect = 0.24
 Identities = 118/150 (78%)
 Strand = Plus / Plus

                                                                       
Query: 1   atttttgttcaacctcttcgctgatccagatatcattcgactccctaggctgttcaggtt 60
           |||||| ||||| || || |||||||||||||| |||||| | || ||  ||||  ||||
Sbjct: 332 atttttattcaatctttttgctgatccagatattattcgattgccaagattgtttcggtt 391

                                                                       
Query: 61  tcttcaaagaccactggccaaacttatatctacttttagagctcctaagagtaaagaggg 120
           | | ||||| ||||||||  |  | ||||||  |  |||||| || ||||| ||||| ||
Sbjct: 392 tttgcaaaggccactggcgcagttcatatctgttgctagagcacccaagagcaaagaagg 451

                                         
Query: 121 gtatgcctcaattggtggtggatcacctct 150
            ||||| |||||||||||||| || |||||
Sbjct: 452 ttatgcttcaattggtggtggttctcctct 481



 Score = 42.1 bits (21), Expect = 0.95
 Identities = 47/56 (83%)
 Strand = Plus / Plus

                                                                   
Query: 224 tatgttggaatgcggnattggtacccattcaccgaagaagccattgatcagattaa 279
           |||||||| |||||  | ||| | |||||||| || ||||||||||| ||||||||
Sbjct: 555 tatgttggtatgcgctactggcatccattcacagaggaagccattgagcagattaa 610

>gb|AC161516.9| Mus musculus chromosome 5, clone RP23-130C18, complete sequence
          Length = 226006

 Score = 42.1 bits (21), Expect = 0.95
 Identities = 24/25 (96%)
 Strand = Plus / Plus

                                       
Query: 195    taaaaagtaagaacttggaagcaga 219
              ||||||||||||||||| |||||||
Sbjct: 159141 taaaaagtaagaacttgaaagcaga 159165

>gb|AC133874.10| Mus musculus chromosome 19, clone RP23-81N16, complete sequence
          Length = 215113

 Score = 40.1 bits (20), Expect = 3.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 200   agtaagaacttggaagcaga 219
             ||||||||||||||||||||
Sbjct: 34883 agtaagaacttggaagcaga 34902

>emb|AL023775.1|HS497J21 Human DNA sequence from clone RP3-497J21 on chromosome 6q26-27 Contains
             part of a variant of the RPS6KA2 gene for ribosomal
             protein S6 kinase, 90kD, polypeptide 2 (RSK3) and a IGF-II
             mRNA-binding protein 3 (KOC1) pseudogene, complete
             sequence
          Length = 129432

 Score = 40.1 bits (20), Expect = 3.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 164   gacgagcaggcaaatgcttt 183
             ||||||||||||||||||||
Sbjct: 10809 gacgagcaggcaaatgcttt 10790

>gb|AC157823.2| Mus musculus BAC clone RP23-382L21 from chromosome 12, complete
             sequence
          Length = 203726

 Score = 40.1 bits (20), Expect = 3.8
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 195   taaaaagtaagaacttggaa 214
             ||||||||||||||||||||
Sbjct: 72027 taaaaagtaagaacttggaa 72008

>gb|AF019664.1|AF019664 Homo sapiens cosmids 52b3, 189g5, ICRFc109N244QD2, P1 255h6, and PAC
            pDJ84g15 from chromosome 6q27 encompassing markers D6S193
            and D6S297, complete sequence
          Length = 68940

 Score = 40.1 bits (20), Expect = 3.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                
Query: 164  gacgagcaggcaaatgcttt 183
            ||||||||||||||||||||
Sbjct: 8941 gacgagcaggcaaatgcttt 8960

>gb|AC115781.9| Mus musculus chromosome 19, clone RP23-167K23, complete sequence
          Length = 210190

 Score = 40.1 bits (20), Expect = 3.8
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 200    agtaagaacttggaagcaga 219
              ||||||||||||||||||||
Sbjct: 203262 agtaagaacttggaagcaga 203281


  Database: nt
    Posted date:  May 29, 2006 11:10 AM
  Number of letters in database: 3,984,495,279
  Number of sequences in database:  917,343
  
  Database: /shigen/export/home/twatanab/db/nt/nt.01
    Posted date:  May 29, 2006 11:16 AM
  Number of letters in database: 3,988,174,986
  Number of sequences in database:  835,257
  
  Database: /shigen/export/home/twatanab/db/nt/nt.02
    Posted date:  May 29, 2006 11:21 AM
  Number of letters in database: 3,991,246,324
  Number of sequences in database:  771,481
  
  Database: /shigen/export/home/twatanab/db/nt/nt.03
    Posted date:  May 29, 2006 11:27 AM
  Number of letters in database: 3,990,718,311
  Number of sequences in database:  977,174
  
  Database: /shigen/export/home/twatanab/db/nt/nt.04
    Posted date:  May 29, 2006 11:29 AM
  Number of letters in database: 1,278,410,368
  Number of sequences in database:  400,813
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 2,524,476
Number of Sequences: 3902068
Number of extensions: 2524476
Number of successful extensions: 40570
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 40517
Number of HSP's gapped (non-prelim): 52
length of query: 287
length of database: 17,233,045,268
effective HSP length: 22
effective length of query: 265
effective length of database: 17,147,199,772
effective search space: 4544007939580
effective search space used: 4544007939580
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)