ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bags34k17
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GSH1_ARATH (P46309) Glutamate--cysteine ligase, chloroplast prec... 127 1e-29
2GSH1_BRAJU (O23736) Glutamate--cysteine ligase, chloroplast prec... 123 2e-28
3GSH1_LYCES (O22493) Glutamate--cysteine ligase, chloroplast prec... 118 6e-27
4GSH1_MEDTR (Q9ZNX6) Glutamate--cysteine ligase, chloroplast prec... 116 2e-26
5NUDH_ZYMMO (Q9RH11) Probable (di)nucleoside polyphosphate hydrol... 34 0.20
6ACDS_MEGEL (Q06319) Acyl-CoA dehydrogenase, short-chain specific... 32 1.0
7CCNB3_CANFA (Q659K0) G2/mitotic-specific cyclin-B3 31 1.7
8IKAR_MOUSE (Q03267) DNA-binding protein Ikaros (Lymphoid transcr... 30 2.9
9U520_CAEEL (Q9U2G0) Putative U5 small nuclear ribonucleoprotein ... 30 3.8
10VG29_BPMU (Q9T1W5) Protein gp29 30 3.8
11SYFB_PSEPK (Q88K22) Phenylalanyl-tRNA synthetase beta chain (EC ... 29 5.0
12CHIA_BOVIN (Q95M17) Acidic mammalian chitinase precursor (EC 3.2... 29 6.6
13MTMR9_MOUSE (Q9Z2D0) Myotubularin-related protein 9 28 8.6

>GSH1_ARATH (P46309) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 522

 Score =  127 bits (320), Expect = 1e-29
 Identities = 61/78 (78%), Positives = 69/78 (88%)
 Frame = +2

Query: 2   PVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNL 181
           PVTGLKTPFRDG ++ +AEDVL+LAK+GLERRGYKE GFL  VDEVVRTGVTPAEKLL +
Sbjct: 445 PVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDEVVRTGVTPAEKLLEM 504

Query: 182 YETKWQRSVDPVFEELLY 235
           Y  +W +SVDPVFEELLY
Sbjct: 505 YNGEWGQSVDPVFEELLY 522



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>GSH1_BRAJU (O23736) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 514

 Score =  123 bits (309), Expect = 2e-28
 Identities = 59/78 (75%), Positives = 68/78 (87%)
 Frame = +2

Query: 2   PVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNL 181
           PVTGLKTPFRDG ++ +AEDVL+LAK+GLERRGYKEVGFL  V EVVRTGVTPAE LL +
Sbjct: 437 PVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEVGFLNAVTEVVRTGVTPAENLLEM 496

Query: 182 YETKWQRSVDPVFEELLY 235
           Y  +W +SVDPVF+ELLY
Sbjct: 497 YNGEWGQSVDPVFQELLY 514



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>GSH1_LYCES (O22493) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 523

 Score =  118 bits (296), Expect = 6e-27
 Identities = 56/78 (71%), Positives = 66/78 (84%)
 Frame = +2

Query: 2   PVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNL 181
           P +GLKTPFRDG +  +A+DV++LAK GLERRG+KE GFL EV EVV+TGVTPAEKLL L
Sbjct: 446 PKSGLKTPFRDGLLMHVAQDVVKLAKEGLERRGFKETGFLNEVAEVVKTGVTPAEKLLEL 505

Query: 182 YETKWQRSVDPVFEELLY 235
           Y  KW +SVDP+FEELLY
Sbjct: 506 YHGKWGQSVDPIFEELLY 523



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>GSH1_MEDTR (Q9ZNX6) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 508

 Score =  116 bits (291), Expect = 2e-26
 Identities = 56/77 (72%), Positives = 67/77 (87%)
 Frame = +2

Query: 5   VTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNLY 184
           VTGLKTPFRDG ++ +AE+VL+LAK+GLERRG+KE GFL  V EVVRTGVTPAE+LL LY
Sbjct: 432 VTGLKTPFRDGLLKHVAEEVLELAKDGLERRGFKESGFLNAVAEVVRTGVTPAERLLELY 491

Query: 185 ETKWQRSVDPVFEELLY 235
             KW++SVD VF+ELLY
Sbjct: 492 HGKWEQSVDHVFDELLY 508



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>NUDH_ZYMMO (Q9RH11) Probable (di)nucleoside polyphosphate hydrolase (EC|
           3.6.1.-)
          Length = 155

 Score = 33.9 bits (76), Expect = 0.20
 Identities = 17/50 (34%), Positives = 28/50 (56%)
 Frame = +2

Query: 47  DLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNLYETKW 196
           D+ E+  Q+ + GLE +   EVG LRE++E   TG+ P    +  +  +W
Sbjct: 28  DMKEEAWQMPQGGLEAKETPEVGVLRELEE--ETGIPPRMVAIISHTKEW 75



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>ACDS_MEGEL (Q06319) Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)|
           (SCAD) (Butyryl-CoA dehydrogenase) (BCAD)
          Length = 383

 Score = 31.6 bits (70), Expect = 1.0
 Identities = 30/117 (25%), Positives = 50/117 (42%), Gaps = 8/117 (6%)
 Frame = +2

Query: 32  DGYVRDLAEDVLQLAKNGLER--------RGYKEVGFLREVDEVVRTGVTPAEKLLNLYE 187
           D  + D+ +D L+LA +  E+        R +K +     +DE++  G+T A      +E
Sbjct: 2   DFNLTDIQQDFLKLAHDFGEKKLAPTVTERDHKGIYDKELIDELLSLGITGA-----YFE 56

Query: 188 TKWQRSVDPVFEELLY*YTESHITSVDAGQGGQGLQTVSLYKNPVLNSTSHLTRETY 358
            K+  S D   + L Y      +   DAG       TVSL  NP+    +   +E +
Sbjct: 57  EKYGGSGDDGGDVLSYILAVEELAKYDAGVAITLSATVSLCANPIWQFGTEAQKEKF 113



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>CCNB3_CANFA (Q659K0) G2/mitotic-specific cyclin-B3|
          Length = 1330

 Score = 30.8 bits (68), Expect = 1.7
 Identities = 13/29 (44%), Positives = 14/29 (48%)
 Frame = -3

Query: 321  TGFLYRDTV*RPCPPCPASTEVICDSVYQ 235
            T FL       PCPPC      ICD +YQ
Sbjct: 1152 TAFLIAAKFEEPCPPCVDDFLYICDDIYQ 1180



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>IKAR_MOUSE (Q03267) DNA-binding protein Ikaros (Lymphoid transcription factor|
           LyF-1)
          Length = 517

 Score = 30.0 bits (66), Expect = 2.9
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = +1

Query: 286 GSSDCIPVQKSRPQFHKPPDEG 351
           GSS+ +PV  S  Q HKPP +G
Sbjct: 332 GSSEVVPVISSMYQLHKPPSDG 353



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>U520_CAEEL (Q9U2G0) Putative U5 small nuclear ribonucleoprotein 200 kDa helicase|
            (EC 3.6.1.-)
          Length = 2145

 Score = 29.6 bits (65), Expect = 3.8
 Identities = 19/80 (23%), Positives = 31/80 (38%), Gaps = 3/80 (3%)
 Frame = +2

Query: 56   EDVLQLAKNGLERRGYKEVGFLREVD---EVVRTGVTPAEKLLNLYETKWQRSVDPVFEE 226
            E+V+  A NG  +    E+  LR  +   E     +TP E +       W+R ++P    
Sbjct: 1338 ENVIVCAPNGSGKTAIAELAVLRHFENTPEAKAVYITPMEDMATKVYADWKRRLEPAIGH 1397

Query: 227  LLY*YTESHITSVDAGQGGQ 286
             +   T      +   Q GQ
Sbjct: 1398 TIVLLTGEQTMDLKLAQRGQ 1417



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>VG29_BPMU (Q9T1W5) Protein gp29|
          Length = 512

 Score = 29.6 bits (65), Expect = 3.8
 Identities = 14/52 (26%), Positives = 27/52 (51%)
 Frame = +2

Query: 80  NGLERRGYKEVGFLREVDEVVRTGVTPAEKLLNLYETKWQRSVDPVFEELLY 235
           NG ++        + + D++ R GV+P +         WQRSVDP+ + +++
Sbjct: 421 NGSQKEAALSAEDIPQEDDIDRMGVSPED---------WQRSVDPLLKPVIF 463



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>SYFB_PSEPK (Q88K22) Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)|
           (Phenylalanine--tRNA ligase beta chain) (PheRS)
          Length = 793

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
 Frame = +3

Query: 159 LLRSF*IYTRRSGSAAWTPSSRSCYIDTLNHI*LRWTLDKVDRVFRLYPCTKIPSSIPQA 338
           LL +  + T +SG   WT S  S   D      +   +D ++ + RLY    +P   PQA
Sbjct: 429 LLNALELTTTKSGEGQWTVSVPSHRFD------ISLEVDLIEELARLYGYNNLPVRYPQA 482

Query: 339 --T*RGKP 356
               +GKP
Sbjct: 483 RLAPQGKP 490



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>CHIA_BOVIN (Q95M17) Acidic mammalian chitinase precursor (EC 3.2.1.14)|
           (AMCase) (Chitin-binding protein b04) (CBPb04)
          Length = 472

 Score = 28.9 bits (63), Expect = 6.6
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +1

Query: 211 PRLRGVVILIH*ITYNFGGRWTRWTGSSDCIPVQKSRPQFHKPPDEGN 354
           P+L   +  IH +TY+F G W  +TG        ++ P +  P D G+
Sbjct: 198 PQLSQYLDFIHVMTYDFHGSWEGYTG--------ENSPLYKYPTDTGS 237



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>MTMR9_MOUSE (Q9Z2D0) Myotubularin-related protein 9|
          Length = 545

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 15/35 (42%), Positives = 22/35 (62%)
 Frame = -2

Query: 298 SLKTLSTLSSVHRSYM*FSVSI*QLLEDGVHAALP 194
           S++ LSTL SV   Y  F   + +++EDG H+ LP
Sbjct: 95  SIEALSTLDSVTLMYPFFYRPMFEVIEDGWHSFLP 129


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,201,452
Number of Sequences: 219361
Number of extensions: 1306073
Number of successful extensions: 3652
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 3572
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3651
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2909956200
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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