Clone Information

BLAST Search Result

Clone Name	bags31k04
Clone Library Name	barley_pub

No.	Definition	Score (bits)	E Value
1	RECA_CAMLA (Q93R37) Protein recA (Recombinase A)	32	0.54
2	SP100_HUMAN (P23497) Nuclear autoantigen Sp-100 (Speckled 100 kD...	31	0.70
3	FKBP4_ASPOR (Q2UN37) FK506-binding protein 4 (EC 5.2.1.8) (Pepti...	31	0.70
4	RP32_PSEAE (P42378) RNA polymerase sigma-32 factor	30	1.6
5	CSN8_BRARE (Q7ZUZ0) COP9 signalosome complex subunit 8 (Signalos...	29	2.7
6	SYQ_HUMAN (P47897) Glutaminyl-tRNA synthetase (EC 6.1.1.18) (Glu...	29	3.5
7	HNF3B_MOUSE (P35583) Hepatocyte nuclear factor 3-beta (HNF-3B) (...	28	4.5
8	FOXA2_BRARE (Q07342) Forkhead box protein A2 (Axial protein)	28	4.5
9	HNF3B_RAT (P32182) Hepatocyte nuclear factor 3-beta (HNF-3B) (Fo...	28	4.5
10	HNF3B_HUMAN (Q9Y261) Hepatocyte nuclear factor 3-beta (HNF-3B) (...	28	4.5
11	ENV_HTL1N (Q03816) Env polyprotein precursor [Contains: Surface ...	28	4.5
12	ENV_HTL1M (P23064) Env polyprotein precursor [Contains: Surface ...	28	4.5
13	ENV_HTL1F (Q03817) Env polyprotein precursor [Contains: Surface ...	28	4.5
14	ENV_HTL1A (P03381) Env polyprotein precursor [Contains: Surface ...	28	4.5
15	HNF3B_ORYLA (O42097) Hepatocyte nuclear factor 3-beta (HNF-3B) (...	28	4.5
16	FKH2_XENLA (P32315) XFKH2 protein	28	5.9
17	CD45_RAT (P04157) Leukocyte common antigen variant 4 precursor (...	28	5.9
18	PLS_STAAU (P80544) Surface protein precursor (Plasmin-sensitive ...	28	5.9
19	HNF3A_RAT (P23512) Hepatocyte nuclear factor 3-alpha (HNF-3A) (F...	28	5.9
20	SYCP2_RAT (O70608) Synaptonemal complex protein 2 (SCP-2) (Synap...	28	5.9
21	HNF3A_MOUSE (P35582) Hepatocyte nuclear factor 3-alpha (HNF-3A) ...	28	5.9
22	HNF3A_HUMAN (P55317) Hepatocyte nuclear factor 3-alpha (HNF-3A) ...	28	5.9
23	MA2A1_HUMAN (Q16706) Alpha-mannosidase 2 (EC 3.2.1.114) (Alpha-m...	28	7.8
24	ENV_HTL1C (P14075) Env polyprotein precursor [Contains: Surface ...	28	7.8

>RECA_CAMLA (Q93R37) Protein recA (Recombinase A)|
          Length = 344

 Score = 31.6 bits (70), Expect = 0.54
 Identities = 18/62 (29%), Positives = 31/62 (50%)
 Frame = +3

Query: 33  REIRQIAEKMDRIVSFEHPVQSNDDKSAYESPCSTTGTARVKYVRGVAVPLGKTKSLVER 212
           RE+  I  KM+  V F + ++       Y +P +TTG   +K+   V + + KT +L + 
Sbjct: 175 RELTGIVHKMNTTVIFINQIRMKIGMMGYGTPETTTGGNALKFYSSVRLDVRKTATLKQN 234

Query: 213 FE 218
            E
Sbjct: 235 DE 236

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>SP100_HUMAN (P23497) Nuclear autoantigen Sp-100 (Speckled 100 kDa) (Nuclear|
           dot-associated Sp100 protein) (Lysp100b)
          Length = 879

 Score = 31.2 bits (69), Expect = 0.70
 Identities = 16/46 (34%), Positives = 30/46 (65%), Gaps = 4/46 (8%)
 Frame = +3

Query: 9   EESEG-TDKREIRQI---AEKMDRIVSFEHPVQSNDDKSAYESPCS 134
           E+SEG TD  E  ++   A + + +++ ++P++SND+K   E+ CS
Sbjct: 329 EDSEGSTDVDEPLEVFISAPRSEPVINNDNPLESNDEKEGQEATCS 374

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>FKBP4_ASPOR (Q2UN37) FK506-binding protein 4 (EC 5.2.1.8) (Peptidyl-prolyl|
           cis-trans isomerase) (PPIase) (Rotamase)
          Length = 470

 Score = 31.2 bits (69), Expect = 0.70
 Identities = 21/59 (35%), Positives = 31/59 (52%)
 Frame = +3

Query: 3   KKEESEGTDKREIRQIAEKMDRIVSFEHPVQSNDDKSAYESPCSTTGTARVKYVRGVAV 179
           KKE  EG + +++ Q A+ ++     + P  S  DK   E    TTGT  VK V+GV +
Sbjct: 321 KKEAKEGKEAKKV-QFAKNLE-----QGPTPSGQDKKPAEQ---TTGTLGVKEVKGVKI 370

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>RP32_PSEAE (P42378) RNA polymerase sigma-32 factor|
          Length = 284

 Score = 30.0 bits (66), Expect = 1.6
 Identities = 14/39 (35%), Positives = 22/39 (56%)
 Frame = +3

Query: 12  ESEGTDKREIRQIAEKMDRIVSFEHPVQSNDDKSAYESP 128
           ES G + RE+R++  ++        P    DD+SAY+SP
Sbjct: 160 ESLGVEPREVREMESRLTGQDMAFDPAADADDESAYQSP 198

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>CSN8_BRARE (Q7ZUZ0) COP9 signalosome complex subunit 8 (Signalosome subunit 8)|
          Length = 191

 Score = 29.3 bits (64), Expect = 2.7
 Identities = 15/41 (36%), Positives = 20/41 (48%)
 Frame = -3

Query: 244 MLEVELSLFSNLSTRDLVFPNGTATPLTYLTLAVPVVEHGD 122
           M E++  L     T++L  P G ATP  Y  L V  + H D
Sbjct: 7   MAELDEKLLLQFETQELEAPGGIATPQVYSQLLVLYLLHND 47

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>SYQ_HUMAN (P47897) Glutaminyl-tRNA synthetase (EC 6.1.1.18) (Glutamine--tRNA|
           ligase) (GlnRS)
          Length = 775

 Score = 28.9 bits (63), Expect = 3.5
 Identities = 15/51 (29%), Positives = 23/51 (45%)
 Frame = -3

Query: 196 LVFPNGTATPLTYLTLAVPVVEHGDSYADLSSFDCTGCSNDTILSIFSAIC 44
           LV  +G   P+ Y     P    GD +    ++D T C  D+I  I  ++C
Sbjct: 406 LVMEDGKMDPVAYRVKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLC 456

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>HNF3B_MOUSE (P35583) Hepatocyte nuclear factor 3-beta (HNF-3B) (Forkhead box|
           protein A2)
          Length = 459

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -2

Query: 194 SFS*RHSDSSNIFDPGCSCRRAR*FIC 114
           SF   H DS N+F+ GC  RR + F C
Sbjct: 231 SFWTLHPDSGNMFENGCYLRRQKRFKC 257

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>FOXA2_BRARE (Q07342) Forkhead box protein A2 (Axial protein)|
          Length = 409

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -2

Query: 194 SFS*RHSDSSNIFDPGCSCRRAR*FIC 114
           SF   H DS N+F+ GC  RR + F C
Sbjct: 223 SFWTLHPDSGNMFENGCYLRRQKRFKC 249

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>HNF3B_RAT (P32182) Hepatocyte nuclear factor 3-beta (HNF-3B) (Forkhead box|
           protein A2)
          Length = 458

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -2

Query: 194 SFS*RHSDSSNIFDPGCSCRRAR*FIC 114
           SF   H DS N+F+ GC  RR + F C
Sbjct: 230 SFWTLHPDSGNMFENGCYLRRQKRFKC 256

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>HNF3B_HUMAN (Q9Y261) Hepatocyte nuclear factor 3-beta (HNF-3B) (Forkhead box|
           protein A2)
          Length = 457

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -2

Query: 194 SFS*RHSDSSNIFDPGCSCRRAR*FIC 114
           SF   H DS N+F+ GC  RR + F C
Sbjct: 231 SFWTLHPDSGNMFENGCYLRRQKRFKC 257

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>ENV_HTL1N (Q03816) Env polyprotein precursor [Contains: Surface glycoprotein|
           GP46; Coat protein GP21 (Transmembrane glycoprotein
           GP21)]
          Length = 488

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 21/70 (30%), Positives = 35/70 (50%)
 Frame = -3

Query: 229 LSLFSNLSTRDLVFPNGTATPLTYLTLAVPVVEHGDSYADLSSFDCTGCSNDTILSIFSA 50
           LS +  L + ++  P+ ++TPL Y +LA+P             F+ T C +  I +I S+
Sbjct: 234 LSTWHVLYSPNVSVPSSSSTPLLYPSLALPAPHL------TLPFNWTHCFDPQIQAIVSS 287

Query: 49  ICLISLLSVP 20
            C  SL+  P
Sbjct: 288 PCHNSLILPP 297

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>ENV_HTL1M (P23064) Env polyprotein precursor [Contains: Surface glycoprotein|
           GP46; Coat protein GP21 (Transmembrane glycoprotein
           GP21)]
          Length = 488

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 21/70 (30%), Positives = 35/70 (50%)
 Frame = -3

Query: 229 LSLFSNLSTRDLVFPNGTATPLTYLTLAVPVVEHGDSYADLSSFDCTGCSNDTILSIFSA 50
           LS +  L + ++  P+ ++TPL Y +LA+P             F+ T C +  I +I S+
Sbjct: 234 LSTWHVLYSPNVSVPSSSSTPLLYPSLALPAPHL------TLPFNWTHCFDPQIQAIVSS 287

Query: 49  ICLISLLSVP 20
            C  SL+  P
Sbjct: 288 PCHNSLILPP 297

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>ENV_HTL1F (Q03817) Env polyprotein precursor [Contains: Surface glycoprotein|
           GP46; Coat protein GP21 (Transmembrane glycoprotein
           GP21)]
          Length = 488

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 21/70 (30%), Positives = 35/70 (50%)
 Frame = -3

Query: 229 LSLFSNLSTRDLVFPNGTATPLTYLTLAVPVVEHGDSYADLSSFDCTGCSNDTILSIFSA 50
           LS +  L + ++  P+ ++TPL Y +LA+P             F+ T C +  I +I S+
Sbjct: 234 LSTWHVLYSPNVSVPSSSSTPLLYPSLALPAPHL------TLPFNWTHCFDPQIQAIVSS 287

Query: 49  ICLISLLSVP 20
            C  SL+  P
Sbjct: 288 PCHNSLILPP 297

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>ENV_HTL1A (P03381) Env polyprotein precursor [Contains: Surface glycoprotein|
           GP46; Coat protein GP21 (Transmembrane glycoprotein
           GP21)]
          Length = 488

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 21/70 (30%), Positives = 35/70 (50%)
 Frame = -3

Query: 229 LSLFSNLSTRDLVFPNGTATPLTYLTLAVPVVEHGDSYADLSSFDCTGCSNDTILSIFSA 50
           LS +  L + ++  P+ ++TPL Y +LA+P             F+ T C +  I +I S+
Sbjct: 234 LSTWHVLYSPNVSVPSSSSTPLLYPSLALPAPHL------TLPFNWTHCFDPQIQAIVSS 287

Query: 49  ICLISLLSVP 20
            C  SL+  P
Sbjct: 288 PCHNSLILPP 297

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>HNF3B_ORYLA (O42097) Hepatocyte nuclear factor 3-beta (HNF-3B) (Me-HNF3B)|
           (Forkhead box protein A2)
          Length = 415

 Score = 28.5 bits (62), Expect = 4.5
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -2

Query: 194 SFS*RHSDSSNIFDPGCSCRRAR*FIC 114
           SF   H DS N+F+ GC  RR + F C
Sbjct: 222 SFWTLHPDSGNMFENGCYLRRQKRFKC 248

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>FKH2_XENLA (P32315) XFKH2 protein|
          Length = 427

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -2

Query: 179 HSDSSNIFDPGCSCRRAR*FIC 114
           H DS N+F+ GC  RR + F C
Sbjct: 234 HPDSGNMFENGCYLRRQKRFKC 255

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>CD45_RAT (P04157) Leukocyte common antigen variant 4 precursor (EC 3.1.3.48)|
           (L-CA) (CD45 antigen) (T200) (Fragment)
          Length = 1255

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 20/71 (28%), Positives = 32/71 (45%)
 Frame = -3

Query: 253 EQSMLEVELSLFSNLSTRDLVFPNGTATPLTYLTLAVPVVEHGDSYADLSSFDCTGCSND 74
           E +  E EL      S +++   NG+ TP   + L VP   H        +F+ T C+ D
Sbjct: 209 EYTDCEKELKNLPECSQKNVTLSNGSCTPDKIINLDVPPGTH--------NFNLTNCTPD 260

Query: 73  TILSIFSAICL 41
             +   ++ICL
Sbjct: 261 --IEANTSICL 269

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>PLS_STAAU (P80544) Surface protein precursor (Plasmin-sensitive surface|
           protein) (230 kDa cell-wall protein)
          Length = 1637

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
 Frame = +3

Query: 12  ESEGTDKREIRQI--AEKMDRIVSFEHPVQSNDDKSAYESP 128
           ++EGTDK E  +   AE+ D+  + E P     DK+  E+P
Sbjct: 130 KAEGTDKVETEEAPKAEETDKATTEEAPKAEETDKATEEAP 170

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>HNF3A_RAT (P23512) Hepatocyte nuclear factor 3-alpha (HNF-3A) (Forkhead box|
           protein A1)
          Length = 466

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -2

Query: 179 HSDSSNIFDPGCSCRRAR*FIC 114
           H DS N+F+ GC  RR + F C
Sbjct: 247 HPDSGNMFENGCYLRRQKRFKC 268

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>SYCP2_RAT (O70608) Synaptonemal complex protein 2 (SCP-2) (Synaptonemal complex|
            lateral element protein) (rnSCP2)
          Length = 1505

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 21/56 (37%), Positives = 30/56 (53%)
 Frame = +1

Query: 103  MTSQHMNHRARRQEQPGSNMLEESLCR*EKLSLLLRDLKRERVLLLALIVHRQLAF 270
            M  Q   HR    ++    +L+E L + EK S  LRDL++E V +   IVH+  AF
Sbjct: 1374 MACQARGHRDENIDKFQVTLLDE-LEKVEKDSQTLRDLEKEFVDIEEKIVHKMRAF 1428

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>HNF3A_MOUSE (P35582) Hepatocyte nuclear factor 3-alpha (HNF-3A) (Forkhead box|
           protein A1)
          Length = 468

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -2

Query: 179 HSDSSNIFDPGCSCRRAR*FIC 114
           H DS N+F+ GC  RR + F C
Sbjct: 247 HPDSGNMFENGCYLRRQKRFKC 268

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>HNF3A_HUMAN (P55317) Hepatocyte nuclear factor 3-alpha (HNF-3A) (Forkhead box|
           protein A1)
          Length = 473

 Score = 28.1 bits (61), Expect = 5.9
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -2

Query: 179 HSDSSNIFDPGCSCRRAR*FIC 114
           H DS N+F+ GC  RR + F C
Sbjct: 248 HPDSGNMFENGCYLRRQKRFKC 269

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>MA2A1_HUMAN (Q16706) Alpha-mannosidase 2 (EC 3.2.1.114) (Alpha-mannosidase II)|
           (Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase)
           (MAN II) (Golgi alpha-mannosidase II) (Mannosidase alpha
           class 2A member 1)
          Length = 1144

 Score = 27.7 bits (60), Expect = 7.8
 Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 5/53 (9%)
 Frame = -3

Query: 241 LEVELSLFSNLSTRD-----LVFPNGTATPLTYLTLAVPVVEHGDSYADLSSF 98
           + V+ S +     RD     L  P+G A P  Y T     V HG  Y++++ F
Sbjct: 789 VNVQFSWYGTTIKRDKSGAYLFLPDGNAKPYVYTTPPFVRVTHGRIYSEVTCF 841

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>ENV_HTL1C (P14075) Env polyprotein precursor [Contains: Surface glycoprotein|
           GP46; Coat protein GP21 (Transmembrane glycoprotein
           GP21)]
          Length = 488

 Score = 27.7 bits (60), Expect = 7.8
 Identities = 21/70 (30%), Positives = 35/70 (50%)
 Frame = -3

Query: 229 LSLFSNLSTRDLVFPNGTATPLTYLTLAVPVVEHGDSYADLSSFDCTGCSNDTILSIFSA 50
           LS +  L + ++  P+ ++TPL Y +LA+P             F+ T C +  I +I S+
Sbjct: 234 LSTWHVLYSPNVSVPSPSSTPLLYPSLALPAPHL------TLPFNWTHCFDPQIQAIVSS 287

Query: 49  ICLISLLSVP 20
            C  SL+  P
Sbjct: 288 PCHNSLILPP 297


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,349,597
Number of Sequences: 219361
Number of extensions: 550189
Number of successful extensions: 1694
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1659
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1691
length of database: 80,573,946
effective HSP length: 74
effective length of database: 64,341,232
effective search space used: 1544189568
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

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full-length cDNA and EST

Clone Information

BLAST Search Result