ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbaal19k21 |
|---|---|
| Clone Library Name | barley_pub |
>SYM_ARATH (Q9SVN5) Probable methionyl-tRNA synthetase (EC 6.1.1.10)| (Methionine--tRNA ligase) (MetRS) Length = 797 Score = 84.3 bits (207), Expect(2) = 2e-28 Identities = 41/80 (51%), Positives = 56/80 (70%) Frame = -2 Query: 546 QPFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDLIPSNHRIGKTVHLFEVLND 367 +PFMPSFS +V +QLNL P+ S +DE+GE RPWD++P +HRIG LF+ L + Sbjct: 516 EPFMPSFSCEVFKQLNLPPQ--FSLSDERGEVLLASRPWDILPPSHRIGTPQPLFKELEN 573 Query: 366 DAVDSFRKTFAGSQAERNAR 307 D V +R+ FAGSQ++R AR Sbjct: 574 DEVARYREKFAGSQSDRRAR 593 Score = 61.6 bits (148), Expect(2) = 2e-28 Identities = 29/43 (67%), Positives = 34/43 (79%) Frame = -1 Query: 667 EGNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 EGN YLQ SQFWKLYK+D CA V++T+AGLV+LLA LL F Sbjct: 476 EGNYYLQASQFWKLYKEDKPLCAVVIRTAAGLVHLLAQLLEPF 518
>SYM_ORYSA (Q9ZTS1) Probable methionyl-tRNA synthetase (EC 6.1.1.10)| (Methionine--tRNA ligase) (MetRS) Length = 804 Score = 104 bits (260), Expect = 2e-22 Identities = 47/81 (58%), Positives = 62/81 (76%) Frame = -2 Query: 546 QPFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDLIPSNHRIGKTVHLFEVLND 367 +PFMPSFS +VL QLN+ P+E +SF D+KGE K KRPWD + + H+IGK LF+ L D Sbjct: 518 EPFMPSFSNEVLLQLNMTPEESLSFCDDKGEIAKAKRPWDFVSAGHKIGKPSPLFKELKD 577 Query: 366 DAVDSFRKTFAGSQAERNARA 304 + V+SFR FAGSQAER+++A Sbjct: 578 EEVESFRNKFAGSQAERSSKA 598 Score = 82.8 bits (203), Expect = 8e-16 Identities = 42/64 (65%), Positives = 49/64 (76%), Gaps = 5/64 (7%) Frame = -1 Query: 667 EGNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF-----HAVLLERCLATA 503 +GNAYLQESQFWKLYK+DPA+CA VMKTS G+VYLLACLL F + VLL+ + Sbjct: 478 DGNAYLQESQFWKLYKEDPAACAVVMKTSVGVVYLLACLLEPFMPSFSNEVLLQLNMTPE 537 Query: 502 ESLS 491 ESLS Sbjct: 538 ESLS 541
>SYM_METKA (Q8TX28) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 668 Score = 42.7 bits (99), Expect = 0.001 Identities = 20/42 (47%), Positives = 26/42 (61%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 GN Y QE + WKL +DP CA V++ A +V LA +LA F Sbjct: 450 GNEYFQEHEPWKLKDEDPERCAEVLRGCARIVKALAVMLAPF 491
>SYM_MOUSE (Q68FL6) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 902 Score = 41.6 bits (96), Expect = 0.002 Identities = 26/89 (29%), Positives = 43/89 (48%) Frame = -2 Query: 585 LQLDLSTFSLVC*QPFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDLIPSNHR 406 + ++++ V QP+MP+ S + QL L P A T +P+ HR Sbjct: 746 MAVNMAALLSVMLQPYMPTVSSTIQTQLQLPPAACRILATSFICT---------LPAGHR 796 Query: 405 IGKTVHLFEVLNDDAVDSFRKTFAGSQAE 319 IG LF+ L +D +++ R+ F G QA+ Sbjct: 797 IGTVSPLFQKLENDQIENLRQRFGGGQAK 825
>SYM_HUMAN (P56192) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 900 Score = 40.4 bits (93), Expect = 0.005 Identities = 26/93 (27%), Positives = 44/93 (47%) Frame = -2 Query: 585 LQLDLSTFSLVC*QPFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDLIPSNHR 406 L ++++ V QP+MP+ S + QL L P T +P+ H+ Sbjct: 744 LAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCT---------LPAGHQ 794 Query: 405 IGKTVHLFEVLNDDAVDSFRKTFAGSQAERNAR 307 IG LF+ L +D ++S R+ F G QA+ + + Sbjct: 795 IGTVSPLFQKLENDQIESLRQRFGGGQAKTSPK 827
>SYM_PYRAB (Q9V011) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 722 Score = 30.0 bits (66), Expect(2) = 0.005 Identities = 15/42 (35%), Positives = 18/42 (42%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 GN Y Q WK K+D T + S +V L LL F Sbjct: 466 GNRYFDHKQPWKTAKEDKVRTGTTVNISLQIVKALGILLEPF 507 Score = 29.3 bits (64), Expect(2) = 0.005 Identities = 21/82 (25%), Positives = 36/82 (43%), Gaps = 2/82 (2%) Frame = -2 Query: 546 QPFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDL--IPSNHRIGKTVHLFEVL 373 +PF+P S+ + LNL ++VKR W+ +P+ H++ K LF+ + Sbjct: 505 EPFLPDASEKIWHLLNL---------------DEVKR-WEFRELPAGHKVRKPEILFKKV 548 Query: 372 NDDAVDSFRKTFAGSQAERNAR 307 DD + F + AR Sbjct: 549 TDDQIIYFILNYMAKGNPEGAR 570
>SYMC_SCHPO (Q9UUF2) Probable methionyl-tRNA synthetase, cytoplasmic (EC| 6.1.1.10) (Methionine--tRNA ligase) (MetRS) Length = 782 Score = 30.8 bits (68), Expect(2) = 0.012 Identities = 18/73 (24%), Positives = 33/73 (45%), Gaps = 1/73 (1%) Frame = -2 Query: 543 PFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDL-IPSNHRIGKTVHLFEVLND 367 P+MPS S + +QLN + W+L + HRIG+ +LF +++ Sbjct: 720 PYMPSTSTSIYKQLN-------------APAAAIPDTWELCLLPGHRIGEPEYLFTRIDE 766 Query: 366 DAVDSFRKTFAGS 328 + +R + G+ Sbjct: 767 SMEEEWRSKYGGN 779 Score = 27.3 bits (59), Expect(2) = 0.012 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Frame = -1 Query: 664 GNAYLQESQFW-KLYKQDPASCATVMKTSAGLVYLLACL 551 GN YLQ+++ K Y + CA + + L+YLLA + Sbjct: 679 GNQYLQDNRIDNKCYLYERQKCADAIGYALNLIYLLAAI 717
>SYM_XENLA (Q6PF21) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 905 Score = 37.4 bits (85), Expect = 0.041 Identities = 21/81 (25%), Positives = 38/81 (46%) Frame = -2 Query: 543 PFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDLIPSNHRIGKTVHLFEVLNDD 364 P+MP+ S + +QL + + + D + S H+IG LF+ L +D Sbjct: 756 PYMPTISSVIQEQLLMPQESKVLTTDF----------CCCLQSGHQIGNVSPLFQKLEND 805 Query: 363 AVDSFRKTFAGSQAERNARAN 301 ++S RK F G Q + ++ + Sbjct: 806 QIESLRKRFGGGQVKTESKVS 826
>SYM_PYRFU (Q8U221) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 724 Score = 28.5 bits (62), Expect(2) = 0.069 Identities = 14/42 (33%), Positives = 18/42 (42%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 GN Y + WK K+D T + S +V L LL F Sbjct: 466 GNRYFDYKEPWKTIKEDKTRTGTTINISLQIVKALGILLEPF 507 Score = 26.9 bits (58), Expect(2) = 0.069 Identities = 22/82 (26%), Positives = 35/82 (42%), Gaps = 2/82 (2%) Frame = -2 Query: 546 QPFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDL--IPSNHRIGKTVHLFEVL 373 +PF+P S+ + LNL E+VK W +P+ H++ K LF+ Sbjct: 505 EPFLPDASEKIWHFLNL---------------EEVKT-WRFTELPAGHKVRKPEILFKKA 548 Query: 372 NDDAVDSFRKTFAGSQAERNAR 307 +DD + F + G AR Sbjct: 549 SDDQIIYFILNYMGRNNPDAAR 570
>SYM_SILPO (Q5LTL1) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 572 Score = 36.6 bits (83), Expect = 0.070 Identities = 19/56 (33%), Positives = 26/56 (46%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAFHAVLLERCLATAES 497 GN YLQ + W L+K DP A ++ L+ L A L A F R L ++ Sbjct: 468 GNEYLQSAAPWTLFKTDPVQAAAQVRLGLNLIRLYAVLSAPFIPAASARMLEAMQT 523
>SYM_AERPE (Q9YCY3) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 572 Score = 34.7 bits (78), Expect = 0.26 Identities = 19/43 (44%), Positives = 22/43 (51%) Frame = -1 Query: 667 EGNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 EGNAYL +Q WKL QD + + V LA LLA F Sbjct: 454 EGNAYLNRTQPWKLLSQDREEGVRALSAALYAVKTLAHLLAPF 496
>SYM_CAEEL (Q20970) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 917 Score = 26.6 bits (57), Expect(2) = 0.31 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = -2 Query: 423 IPSNHRIGKTVHLFEVLNDDAVDSFRKTFAGSQ 325 + + H+IG+ LF+ L+ + F+ F GSQ Sbjct: 561 LKAGHKIGQPSPLFQKLDPAQIAEFKAKFGGSQ 593 Score = 26.6 bits (57), Expect(2) = 0.31 Identities = 16/57 (28%), Positives = 26/57 (45%), Gaps = 7/57 (12%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPAS---CATVMKTSAGLVY----LLACLLAAFHAVLLERC 515 GN Y+Q W L K+D T++ +A + Y LL ++ A + E+C Sbjct: 489 GNQYMQAQTPWVLMKKDEEGKKRAGTIIGVAANIAYHVSVLLYPIMPTISATIREQC 545
>SYM_CAUCR (Q9A884) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 569 Score = 32.0 bits (71), Expect = 1.7 Identities = 16/42 (38%), Positives = 22/42 (52%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 GN YLQE+ W K D A +++T+ L L A + A F Sbjct: 464 GNEYLQEAAPWTAIKTDRDRAAVIVRTALNLAALYAKISAPF 505
>SYM_PORGI (Q7MXK7) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 680 Score = 32.0 bits (71), Expect = 1.7 Identities = 23/79 (29%), Positives = 35/79 (44%), Gaps = 1/79 (1%) Frame = -2 Query: 585 LQLDLSTFSLVC*QPFMPSFSKDVLQQLNL-CPKEHISFADEKGETEKVKRPWDLIPSNH 409 L L ++ + +PF+P S ++ L+ CP D G T DL+P H Sbjct: 482 LSLQITANLAIAFEPFLPFSSAKLMSMLSTDCPFGW----DRLGST-------DLLPEGH 530 Query: 408 RIGKTVHLFEVLNDDAVDS 352 +G LFE L D +D+ Sbjct: 531 VLGNPELLFEKLEDSVIDA 549 Score = 29.6 bits (65), Expect = 8.5 Identities = 15/42 (35%), Positives = 21/42 (50%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 GN YL +++ WKL K D AT++ S + LA F Sbjct: 456 GNKYLADTEPWKLAKTDMNRVATILNLSLQITANLAIAFEPF 497
>SYM_CHLTE (P59077) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 703 Score = 32.0 bits (71), Expect = 1.7 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = -1 Query: 661 NAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACL 551 N +L ES+ WK+ K DP + M S L + LA L Sbjct: 467 NRFLTESEPWKVIKVDPEAAGRTMAVSLNLCHTLALL 503
>SYM_STRAW (Q82F60) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 574 Score = 31.6 bits (70), Expect = 2.2 Identities = 17/59 (28%), Positives = 31/59 (52%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAFHAVLLERCLATAESLSE 488 GN+YL+E W K +P A ++T+ L++L + + F + + +A +LSE Sbjct: 467 GNSYLEEKAPWLEIKTNPEGAALTLRTAMNLIHLYSVVSEPF-IPASSKAMRSAFALSE 524
>SYM_BACFR (Q64MP7) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 679 Score = 31.2 bits (69), Expect = 2.9 Identities = 18/49 (36%), Positives = 23/49 (46%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAFHAVLLER 518 GN YL +++ WKL K D T++ S LV LA F ER Sbjct: 456 GNKYLADTEPWKLAKTDMERVGTILNISLQLVANLAIAFEPFLPFSSER 504 Score = 30.0 bits (66), Expect = 6.5 Identities = 21/78 (26%), Positives = 38/78 (48%) Frame = -2 Query: 585 LQLDLSTFSLVC*QPFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDLIPSNHR 406 + L L + +PF+P S+ + Q LN+ + +A E G DL+P+ H+ Sbjct: 482 ISLQLVANLAIAFEPFLPFSSERLRQMLNM---DSFDWA-ELGRN-------DLLPAGHQ 530 Query: 405 IGKTVHLFEVLNDDAVDS 352 + K LFE + D +++ Sbjct: 531 LNKPELLFEKIEDATIEA 548
>SYM_PYRHO (O58721) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 723 Score = 31.2 bits (69), Expect = 2.9 Identities = 16/42 (38%), Positives = 19/42 (45%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 GN Y Q WK K+D A T + S +V L LL F Sbjct: 466 GNKYFDHKQPWKTAKEDRARTGTTVNISLQIVKALGILLEPF 507
>SYM_BACTN (Q8A3M1) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 679 Score = 30.8 bits (68), Expect = 3.8 Identities = 16/42 (38%), Positives = 21/42 (50%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 GN YL +++ WKL K D T++ S LV LA F Sbjct: 456 GNKYLADTEPWKLAKTDMERVGTILNISLQLVANLAIAFEPF 497 Score = 30.0 bits (66), Expect = 6.5 Identities = 20/78 (25%), Positives = 37/78 (47%) Frame = -2 Query: 585 LQLDLSTFSLVC*QPFMPSFSKDVLQQLNLCPKEHISFADEKGETEKVKRPWDLIPSNHR 406 + L L + +PF+P S+ + + LN+ E E ++ R DL+P H+ Sbjct: 482 ISLQLVANLAIAFEPFLPFSSEKLRKMLNM----------ESFEWSELGRN-DLLPVGHQ 530 Query: 405 IGKTVHLFEVLNDDAVDS 352 + K LFE + D +++ Sbjct: 531 LNKPELLFEKIEDSVIEA 548
>SYM_METAC (Q8TIU5) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 712 Score = 30.4 bits (67), Expect = 5.0 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = -1 Query: 664 GNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACL 551 GN Y Q + WKL K+D ++C V+ L L+ + Sbjct: 453 GNTYFQSHEPWKLIKEDRSACGQVIYNCLHLAKALSLI 490
>PP2BC_MOUSE (P48455) Serine/threonine-protein phosphatase 2B catalytic subunit| gamma isoform (EC 3.1.3.16) (Calmodulin-dependent calcineurin A subunit gamma isoform) (Calcineurin, testis-specific catalytic subunit) (CAM-PRP catalytic subunit) Length = 513 Score = 30.4 bits (67), Expect = 5.0 Identities = 24/75 (32%), Positives = 41/75 (54%), Gaps = 2/75 (2%) Frame = -2 Query: 579 LDLSTFSLVC*QPFMPSFSKDVLQQ-LNLCPKEHISFADEKGETEKVKRPWDLIPSNHR- 406 +D+ T+SL PF+ ++L LN+C E ++ DE+G T K ++I + R Sbjct: 343 MDVFTWSL----PFVGEKVTEMLVNILNICSDEEMNVTDEEGATTGRK---EVIKNKIRA 395 Query: 405 IGKTVHLFEVLNDDA 361 IGK +F VL +++ Sbjct: 396 IGKMARVFTVLREES 410
>SYM_RHIME (Q92PX0) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 516 Score = 30.0 bits (66), Expect = 6.5 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = -1 Query: 667 EGNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAF 539 E + Y + W L K DP+ ATV+ +A +V +A LL F Sbjct: 423 ETDRYFAGQEPWALKKTDPSRMATVLYVTAEVVRQVAILLQPF 465
>SYM2_BACCR (Q815U6) Methionyl-tRNA synthetase 2 (EC 6.1.1.10)| (Methionine--tRNA ligase 2) (MetRS 2) Length = 544 Score = 30.0 bits (66), Expect = 6.5 Identities = 17/48 (35%), Positives = 20/48 (41%) Frame = -1 Query: 661 NAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAFHAVLLER 518 N Y E Q WK + DP SC + L+ A LL F ER Sbjct: 448 NKYFDEKQPWKQREDDPVSCEETIYNCVYLIANFANLLEPFLPFSSER 495
>SYM_XANOR (Q5H1J3) Methionyl-tRNA synthetase (EC 6.1.1.10) (Methionine--tRNA| ligase) (MetRS) Length = 694 Score = 29.6 bits (65), Expect = 8.5 Identities = 24/86 (27%), Positives = 36/86 (41%) Frame = -1 Query: 667 EGNAYLQESQFWKLYKQDPASCATVMKTSAGLVYLLACLLAAFHAVLLERCLATAESLSE 488 E N Y+ +++ W + KQD A + GL L L+AA +L C LS Sbjct: 449 EANKYIDDTKPWVIAKQDSADAQLQSVCTQGL-NLFRVLVAALKPILPRTCAEAEAFLSA 507 Query: 487 RAYFFR**KGGD*EGEKALGSHTIKP 410 + + + L SHTI+P Sbjct: 508 PMTSWE-------DVIRPLTSHTIQP 526 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 85,043,898 Number of Sequences: 219361 Number of extensions: 1591510 Number of successful extensions: 3761 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 3617 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3755 length of database: 80,573,946 effective HSP length: 108 effective length of database: 56,882,958 effective search space used: 6427774254 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
