>GLYM_ARATH (Q9SZJ5) Serine hydroxymethyltransferase, mitochondrial precursor|
           (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT)
          Length = 517

 Score = 54.7 bits (130), Expect = 1e-07
 Identities = 31/74 (41%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKE-HGKLLKDFSKGLVNNKDIEN----LKVEVEKFALS 325
           E+DF ++AE+  +AVTI L ++ E  G  LKDF   + ++  I++    L+ EVE+FA  
Sbjct: 443 EEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQ 502

Query: 324 FDMPGFTLESMKYK 283
           F   GF  E+MKYK
Sbjct: 503 FPTIGFEKETMKYK 516

>GLYC_CAEEL (P50432) Serine hydroxymethyltransferase (EC 2.1.2.1) (Serine|
           methylase) (Glycine hydroxymethyltransferase) (SHMT)
           (Glycosylation-related protein 1)
          Length = 507

 Score = 51.6 bits (122), Expect = 1e-06
 Identities = 25/64 (39%), Positives = 37/64 (57%), Gaps = 4/64 (6%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHGKLLKDFSKGLVNN----KDIENLKVEVEKFALSF 322
           E+DFE++ +F+H+ V I      E GK LKDF      N    KD+ +L   VE+F+  F
Sbjct: 440 EQDFEKVGDFIHEGVQIAKKYNAEAGKTLKDFKSFTETNEPFKKDVADLAKRVEEFSTKF 499

Query: 321 DMPG 310
           ++PG
Sbjct: 500 EIPG 503

>GLYC_HUMAN (P34896) Serine hydroxymethyltransferase, cytosolic (EC 2.1.2.1)|
           (Serine methylase) (Glycine hydroxymethyltransferase)
           (SHMT)
          Length = 483

 Score = 48.1 bits (113), Expect = 1e-05
 Identities = 25/65 (38%), Positives = 40/65 (61%), Gaps = 5/65 (7%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHG--KLLKDFSKGLVNNK---DIENLKVEVEKFALS 325
           EKDF+++A F+H+ + + L IQ + G    LK+F + L  +K    ++ L+ EVE FA  
Sbjct: 415 EKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASL 474

Query: 324 FDMPG 310
           F +PG
Sbjct: 475 FPLPG 479

>GLYM_PEA (P34899) Serine hydroxymethyltransferase, mitochondrial precursor|
           (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT)
          Length = 518

 Score = 47.4 bits (111), Expect = 2e-05
 Identities = 28/75 (37%), Positives = 43/75 (57%), Gaps = 5/75 (6%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEH-GKLLKDFSKGLVNNK----DIENLKVEVEKFALS 325
           E+DF ++AE+   AV++ L ++ E  G  LKDF + L  +     +I  LK +VE+FA  
Sbjct: 444 EEDFVKVAEYFDAAVSLALKVKAESKGTKLKDFVEALQTSSYVQSEISKLKHDVEEFAKQ 503

Query: 324 FDMPGFTLESMKYKE 280
           F   GF   +MKY +
Sbjct: 504 FPTIGFEKATMKYNK 518

>GLYM_SOLTU (P50433) Serine hydroxymethyltransferase, mitochondrial precursor|
           (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT)
          Length = 518

 Score = 46.6 bits (109), Expect = 4e-05
 Identities = 28/74 (37%), Positives = 43/74 (58%), Gaps = 5/74 (6%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKE-HGKLLKDFSKGLVNN----KDIENLKVEVEKFALS 325
           E+DF ++A+F   AV I + ++ E  G  LKDF   L ++     +I  L+ +VE++A  
Sbjct: 444 EEDFVKVADFFDAAVKIAVKVKAETQGTKLKDFVATLESSAPIKSEIAKLRHDVEEYAKQ 503

Query: 324 FDMPGFTLESMKYK 283
           F   GF  E+MKYK
Sbjct: 504 FPTIGFEKETMKYK 517

>GLYC_MOUSE (P50431) Serine hydroxymethyltransferase, cytosolic (EC 2.1.2.1)|
           (Serine methylase) (Glycine hydroxymethyltransferase)
           (SHMT)
          Length = 478

 Score = 45.1 bits (105), Expect = 1e-04
 Identities = 23/66 (34%), Positives = 40/66 (60%), Gaps = 6/66 (9%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHG--KLLKDFSKGLVNNKDIEN----LKVEVEKFAL 328
           E+DF+++A F+H+ + + L IQ        LK+F + L  ++ I++    L+ EVE FA 
Sbjct: 409 EEDFQKVAHFIHRGIELTLQIQSHMATKATLKEFKEKLAGDEKIQSAVATLREEVENFAS 468

Query: 327 SFDMPG 310
           +F +PG
Sbjct: 469 NFSLPG 474

>GLYC_RABIT (P07511) Serine hydroxymethyltransferase, cytosolic (EC 2.1.2.1)|
           (Serine methylase) (Glycine hydroxymethyltransferase)
           (SHMT)
          Length = 483

 Score = 44.7 bits (104), Expect = 1e-04
 Identities = 23/66 (34%), Positives = 39/66 (59%), Gaps = 6/66 (9%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHG--KLLKDFSKGLV----NNKDIENLKVEVEKFAL 328
           EKDF+++A F+H+ + + + IQ + G    LK+F + L     + + +  L+ EVE FA 
Sbjct: 414 EKDFQKVAHFIHRGIELTVQIQDDTGPRATLKEFKEKLAGDEKHQRAVRALRQEVESFAA 473

Query: 327 SFDMPG 310
            F +PG
Sbjct: 474 LFPLPG 479

>GLYM_HUMAN (P34897) Serine hydroxymethyltransferase, mitochondrial precursor|
           (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT)
          Length = 504

 Score = 43.9 bits (102), Expect = 2e-04
 Identities = 23/65 (35%), Positives = 39/65 (60%), Gaps = 4/65 (6%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHGKLLKDFSKGLVNNKD----IENLKVEVEKFALSF 322
           E DF ++ +F+ + V I L ++ +  KL +DF   L+ + +    + NL+  VE+FA +F
Sbjct: 438 EDDFRRVVDFIDEGVNIGLEVKSKTAKL-QDFKSFLLKDSETSQRLANLRQRVEQFARAF 496

Query: 321 DMPGF 307
            MPGF
Sbjct: 497 PMPGF 501

>GLYC_SHEEP (P35623) Serine hydroxymethyltransferase, cytosolic (EC 2.1.2.1)|
           (Serine methylase) (Glycine hydroxymethyltransferase)
           (SHMT)
          Length = 483

 Score = 42.0 bits (97), Expect = 9e-04
 Identities = 23/66 (34%), Positives = 37/66 (56%), Gaps = 6/66 (9%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHG--KLLKDFSKGLV----NNKDIENLKVEVEKFAL 328
           E+DF ++A F+H+ + + L IQ   G    LK+F + L     + + +  L+ EVE FA 
Sbjct: 414 EEDFRKVAHFIHRGIELTLQIQDAVGVKATLKEFMEKLAGAEEHQRAVTALRAEVESFAT 473

Query: 327 SFDMPG 310
            F +PG
Sbjct: 474 LFPLPG 479

>GLYM_CANAL (O13425) Serine hydroxymethyltransferase, mitochondrial precursor|
           (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT)
          Length = 493

 Score = 35.0 bits (79), Expect = 0.11
 Identities = 20/64 (31%), Positives = 36/64 (56%), Gaps = 5/64 (7%)
 Frame = -3

Query: 486 KDFEQIAEFLHQAVTICLNIQ-KEHGK----LLKDFSKGLVNNKDIENLKVEVEKFALSF 322
           ++F+++AEF+ QAV I + ++ +E GK    LL  F K    +  ++ L  EV  +   +
Sbjct: 428 EEFDKVAEFIDQAVNIAIELKAQEQGKVPKELLASFKKLADESDKVKQLDKEVVSWVSKY 487

Query: 321 DMPG 310
            +PG
Sbjct: 488 PVPG 491

>GLYD_SCHPO (O13972) Probable serine hydroxymethyltransferase, cytosolic (EC|
           2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT)
          Length = 467

 Score = 34.3 bits (77), Expect = 0.19
 Identities = 21/64 (32%), Positives = 37/64 (57%), Gaps = 6/64 (9%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHGK---LLKDFSKGLVNNK---DIENLKVEVEKFAL 328
           E+DF ++ +++ +A+T   N+QKE  K    LKDF   L   +   ++  L+ EV ++A 
Sbjct: 400 EQDFVRVVDYIDRALTFAANLQKELPKDANKLKDFKAKLGEGEQYPELVQLQKEVAEWAS 459

Query: 327 SFDM 316
           SF +
Sbjct: 460 SFPL 463

>G6PI_MOUSE (P06745) Glucose-6-phosphate isomerase (EC 5.3.1.9) (GPI)|
           (Phosphoglucose isomerase) (PGI) (Phosphohexose
           isomerase) (PHI) (Neuroleukin) (NLK)
          Length = 557

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 15/33 (45%), Positives = 22/33 (66%)
 Frame = -3

Query: 435 LNIQKEHGKLLKDFSKGLVNNKDIENLKVEVEK 337
           LN+   HG +L D+SK LV NK++  + VE+ K
Sbjct: 41  LNLNTNHGHILVDYSKNLV-NKEVMQMLVELAK 72

>IRS2_HUMAN (Q9Y4H2) Insulin receptor substrate 2 (IRS-2)|
          Length = 1338

 Score = 31.2 bits (69), Expect = 1.6
 Identities = 20/42 (47%), Positives = 24/42 (57%), Gaps = 5/42 (11%)
 Frame = +3

Query: 378 SPGPWRSP*AASRAPS-GCSGRWS----LPGGGTRRSARSPS 488
           SPGP R+P + S   S GC GR S    LP GG  + +RS S
Sbjct: 391 SPGPVRAPLSRSHTLSGGCGGRGSKVALLPAGGALQHSRSMS 432

>MRC1_SCHPO (Q9P7T4) Mediator of replication checkpoint protein 1 (DNA|
           replication checkpoint mediator mrc1)
          Length = 1019

 Score = 31.2 bits (69), Expect = 1.6
 Identities = 15/35 (42%), Positives = 22/35 (62%)
 Frame = -3

Query: 432 NIQKEHGKLLKDFSKGLVNNKDIENLKVEVEKFAL 328
           N++KE  K   D S G+VN++DI+ L VE +   L
Sbjct: 271 NVEKEEPKPSVDRSTGIVNSEDIKELSVEDDSLEL 305

>GLYC_SCHPO (Q10104) Probable serine hydroxymethyltransferase, cytosolic (EC|
           2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT)
          Length = 472

 Score = 31.2 bits (69), Expect = 1.6
 Identities = 23/67 (34%), Positives = 33/67 (49%), Gaps = 8/67 (11%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQ----KEHGKLLKDFSKGLVNN----KDIENLKVEVEKF 334
           EKDFE++ E + + V++   I     KE     +DF K  V +     +I  LK EV  +
Sbjct: 406 EKDFERVVELIDEVVSLTKKINEAALKEGKSKFRDF-KAYVGDGSKFSEIAKLKKEVITW 464

Query: 333 ALSFDMP 313
           A  FD P
Sbjct: 465 AGKFDFP 471

>GLYM_CANGA (Q6FQ44) Serine hydroxymethyltransferase, mitochondrial precursor|
           (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT)
          Length = 485

 Score = 30.8 bits (68), Expect = 2.1
 Identities = 16/67 (23%), Positives = 35/67 (52%), Gaps = 7/67 (10%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHGKLLKD-------FSKGLVNNKDIENLKVEVEKFA 331
           E+DF+++AE++  AV + + ++ +     KD       F +    ++ ++ L  EV  + 
Sbjct: 417 EQDFKKVAEYIDNAVKLSIALKSQESADAKDVRSKLNSFKQLCDQSEPVQKLAEEVSSWV 476

Query: 330 LSFDMPG 310
            +F +PG
Sbjct: 477 GTFPVPG 483

>MEF2B_HUMAN (Q02080) Myocyte-specific enhancer factor 2B (Serum response|
           factor-like protein 2) (XMEF2) (RSRFR2)
          Length = 365

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 15/36 (41%), Positives = 17/36 (47%)
 Frame = +3

Query: 360 SQCPCCSPGPWRSP*AASRAPSGCSGRWSLPGGGTR 467
           S  PC SPGPW+S        +GC   W   G G R
Sbjct: 300 SPIPCSSPGPWQSLCGLGPPCAGCP--WPTAGPGRR 333

>VGLB_HSVA1 (Q04463) Glycoprotein B precursor|
          Length = 933

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 19/35 (54%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
 Frame = +3

Query: 378 SPGPWRSP*AASRAPSGCSGRW-SLPGGGTRRSAR 479
           SPGP R P    R P G SGR    PG GTR +AR
Sbjct: 84  SPGPDRPP----RGPGGGSGRRRGSPGNGTRSAAR 114

>CD2A2_MONDO (O77618) Cyclin-dependent kinase inhibitor 2A, isoform 2 (p19ARF)|
          Length = 155

 Score = 30.0 bits (66), Expect = 3.5
 Identities = 20/59 (33%), Positives = 25/59 (42%), Gaps = 6/59 (10%)
 Frame = +3

Query: 327 RGQTSRPRP*GS------QCPCCSPGPWRSP*AASRAPSGCSGRWSLPGGGTRRSARSP 485
           RG T  PRP         +CP   PG   +   + R  +GC G    P  G RR  R+P
Sbjct: 76  RGGTQHPRPDNPHPASPRRCPGGLPGHSDAA-PSGRGAAGCQGLLGSPACGPRRGTRTP 133

>TGT_PROMP (Q7TUG0) Queuine tRNA-ribosyltransferase (EC 2.4.2.29)|
           (tRNA-guanine transglycosylase) (Guanine insertion
           enzyme)
          Length = 372

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 18/79 (22%), Positives = 34/79 (43%)
 Frame = -3

Query: 489 EKDFEQIAEFLHQAVTICLNIQKEHGKLLKDFSKGLVNNKDIENLKVEVEKFALSFDMPG 310
           E D E   E  H  +  C+   ++  + L     G+V       L+    KF  SFD+PG
Sbjct: 155 ENDIEDSLERTHSWLQKCVETHQKSNQALF----GIVQGGKYPRLREHSAKFTSSFDLPG 210

Query: 309 FTLESMKYKE*DDDTYAMV 253
             +  +   E  ++ ++++
Sbjct: 211 IAVGGVSVGEAVEEIHSVI 229

>GP135_HUMAN (Q8IZ08) Probable G-protein coupled receptor 135|
          Length = 494

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 14/29 (48%), Positives = 15/29 (51%)
 Frame = +3

Query: 351 P*GSQCPCCSPGPWRSP*AASRAPSGCSG 437
           P G   P  + GPWR   AASR  S C G
Sbjct: 167 PPGGSAPAAAAGPWRGFCAASRFFSSCFG 195

>MURC_CAMJR (Q5HU47) UDP-N-acetylmuramate--L-alanine ligase (EC 6.3.2.8)|
           (UDP-N-acetylmuramoyl-L-alanine synthetase)
          Length = 431

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 26/80 (32%), Positives = 39/80 (48%), Gaps = 5/80 (6%)
 Frame = -3

Query: 468 AEFLHQAVTICLNIQKEHGKLLKDFSKGLVNNKDIENLKVEVEKFA--LSF---DMPGFT 304
           AEFL  A    +N + E  K  K+ S  L  +KDI+N  + +E F    SF   D+  F 
Sbjct: 193 AEFLDTAKIRVINAEDEFLKNYKNESTKLYPSKDIKNCTMRIENFKPFTSFELKDLGEFK 252

Query: 303 LESMKYKE*DDDTYAMVVSL 244
           +  M Y    D + A++ +L
Sbjct: 253 VFGMGYHLALDASLAILAAL 272

>K0174_HUMAN (P53990) Protein KIAA0174 (Putative MAPK-activating protein PM28)|
          Length = 364

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = -3

Query: 474 QIAEFLHQAVTICLNIQKEHGKLLKDFSKGLVNNKDIENLKVE 346
           ++AE    A  +C    KE+GKL +    G VN++ +  L VE
Sbjct: 113 EVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVE 155

>K0174_MOUSE (Q9CX00) Protein KIAA0174|
          Length = 362

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = -3

Query: 474 QIAEFLHQAVTICLNIQKEHGKLLKDFSKGLVNNKDIENLKVE 346
           ++AE    A  +C    KE+GKL +    G VN++ +  L VE
Sbjct: 113 EVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVE 155

>UCP2_CYPCA (Q9W725) Mitochondrial uncoupling protein 2 (UCP 2)|
          Length = 310

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 4/40 (10%)
 Frame = -1

Query: 173 SPG*FCKRLPPLVLCLPSTSPSYFFVGIV----RVGSWNV 66
           +PG +C  L   V  L    P  F+ G +    R+GSWNV
Sbjct: 247 APGQYCSALNCAVAMLTKEGPKAFYKGFMPSFLRLGSWNV 286

>YT35_STRFR (P20186) Hypothetical 35.5 kDa protein in transposon Tn4556|
          Length = 348

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 22/63 (34%), Positives = 26/63 (41%), Gaps = 10/63 (15%)
 Frame = +3

Query: 324 RRGQTSRPRP*GSQC----PCCSPGPWRSP*------AASRAPSGCSGRWSLPGGGTRRS 473
           RR   S PRP         P   P P R+P       A +RAP+GCS     P   T R 
Sbjct: 260 RRTTPSAPRPSSRPARPPIPAARPPPRRTPGTPRPAAARARAPAGCSPARRTPSAPTDRR 319

Query: 474 ARS 482
            R+
Sbjct: 320 CRA 322

>GRPE_CHLMU (P23575) Protein grpE (HSP-70 cofactor)|
          Length = 190

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = -3

Query: 462 FLHQAVTICLNIQKEHGKLLKDFSKGL-VNNKDIENLKVEVEKF 334
           +LH+AV I        G +L++F+KG  + ++ I   KV+V KF
Sbjct: 134 YLHEAVEIEETTDIPEGTILEEFTKGYKIGDRPIRVAKVKVAKF 177

>G6PI_CRIGR (P50309) Glucose-6-phosphate isomerase (EC 5.3.1.9) (GPI)|
           (Phosphoglucose isomerase) (PGI) (Phosphohexose
           isomerase) (PHI)
          Length = 558

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 13/33 (39%), Positives = 22/33 (66%)
 Frame = -3

Query: 435 LNIQKEHGKLLKDFSKGLVNNKDIENLKVEVEK 337
           LN+   HG +L D+SK LV NK++  + V++ +
Sbjct: 42  LNLNTTHGHILVDYSKNLV-NKEVMQMLVDLAR 73

>RUSC2_MOUSE (Q80U22) RUN and SH3 domain-containing protein 2|
          Length = 1514

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 21/51 (41%), Positives = 25/51 (49%)
 Frame = +3

Query: 333 QTSRPRP*GSQCPCCSPGPWRSP*AASRAPSGCSGRWSLPGGGTRRSARSP 485
           +TSRP P GS  P  S GP+ S    S A + CS     P  GT   + SP
Sbjct: 765 ETSRPSPLGSYSPVRSAGPFGSS-TDSSASTSCS---PPPEQGTAADSVSP 811

>COAA1_CHICK (P08125) Collagen alpha-1(X) chain precursor|
          Length = 674

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 18/48 (37%), Positives = 19/48 (39%)
 Frame = +3

Query: 321 QRRGQTSRPRP*GSQCPCCSPGPWRSP*AASRAPSGCSGRWSLPGGGT 464
           Q RG+   P P G   P   PGP   P   S  P G  G    PG  T
Sbjct: 50  QMRGEQGPPGPPGPIGPRGQPGPAGKPGFGSPGPQGPPGPLGPPGFST 97

>COX10_CANGA (Q6FUG4) Protoheme IX farnesyltransferase, mitochondrial precursor|
           (EC 2.5.1.-) (Heme O synthase)
          Length = 451

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +1

Query: 46  KIIGKYSTFHDPTRTIPTKKYDGDVDGKQS 135
           K+IGKYS  H+ TR I  + Y  +   K +
Sbjct: 24  KVIGKYSAIHEATRKIIVRSYSKETIKKHN 53


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,170,059
Number of Sequences: 219361
Number of extensions: 1518210
Number of successful extensions: 5055
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 4833
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5043
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3478785780
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)