Clone Information

BLAST Search Result

Clone Name	rbags34a05
Clone Library Name	barley_pub

No.	Definition	Score (bits)	E Value
1	NIT1_RAT (Q7TQ94) Nitrilase homolog 1 (EC 3.5.-.-)	69	2e-11
2	NIT1_HUMAN (Q86X76) Nitrilase homolog 1 (EC 3.5.-.-)	68	3e-11
3	DCAS_AGRSK (P60327) N-carbamoyl-D-amino acid hydrolase (EC 3.5.1...	67	4e-11
4	DCAS_AGRTU (Q44185) N-carbamoyl-D-amino acid hydrolase (EC 3.5.1...	67	6e-11
5	NIT3_YEAST (P49954) Probable hydrolase NIT3 (EC 3.5.-.-)	66	1e-10
6	NIT1_MOUSE (Q8VDK1) Nitrilase homolog 1 (EC 3.5.-.-)	65	2e-10
7	BUP1_PONPY (Q5RBM6) Beta-ureidopropionase (EC 3.5.1.6) (Beta-ala...	59	1e-08
8	BUP1_RAT (Q03248) Beta-ureidopropionase (EC 3.5.1.6) (Beta-alani...	57	6e-08
9	BUP1_MOUSE (Q8VC97) Beta-ureidopropionase (EC 3.5.1.6) (Beta-ala...	57	8e-08
10	YAG5_STAAU (P55177) Hypothetical UPF0012 protein in agr operon (...	56	1e-07
11	NFT1_CAEEL (O76463) Nitrilase and fragile histidine triad fusion...	56	1e-07
12	BUP1_HUMAN (Q9UBR1) Beta-ureidopropionase (EC 3.5.1.6) (Beta-ala...	56	1e-07
13	YPQQ_PSEFL (P55176) Hypothetical UPF0012 protein in pqqF 5'regio...	54	4e-07
14	NFT1_DROME (O76464) Nitrilase and fragile histidine triad fusion...	54	5e-07
15	YAUB_SCHPO (Q10166) Hypothetical UPF0012 protein C26A3.11 in chr...	53	9e-07
16	Y601_SYNY3 (P55175) Hypothetical UPF0012 protein sll0601 (EC 3.5...	53	9e-07
17	YHCX_BACSU (P54608) Hypothetical UPF0012 protein yhcX (EC 3.5.-.-)	49	2e-05
18	YAG5_STALU (P55178) Hypothetical UPF0012 protein in agr operon (...	48	4e-05
19	YBEM_ECOLI (P39874) Hypothetical UPF0012 protein ybeM (EC 3.5.-.-)	46	1e-04
20	YBEM_ECO57 (P58054) Hypothetical UPF0012 protein ybeM (EC 3.5.-.-)	46	1e-04
21	Y480_MYCTU (Q11146) Hypothetical UPF0012 protein Rv0480c/MT0498 ...	46	1e-04
22	AMIF_BRAJA (Q89H51) Formamidase (EC 3.5.1.49) (Formamide amidohy...	44	4e-04
23	NADE_RHOCA (Q03638) Glutamine-dependent NAD(+) synthetase (EC 6....	44	5e-04
24	NRL4_ARATH (P46011) Nitrilase 4 (EC 3.5.5.1)	44	7e-04
25	NIT2_YEAST (P47016) Probable hydrolase NIT2 (EC 3.5.-.-)	43	9e-04
26	NRL4_TOBAC (Q42965) Nitrilase 4 (EC 3.5.5.1)	43	9e-04
27	NRL_BACSX (P82605) Nitrilase (EC 3.5.5.1)	42	0.003
28	AMIF_PSESM (Q887D9) Formamidase (EC 3.5.1.49) (Formamide amidohy...	42	0.003
29	AMIF_BACCR (P59701) Formamidase (EC 3.5.1.49) (Formamide amidohy...	38	0.038
30	AMIF_BACAN (P59700) Formamidase (EC 3.5.1.49) (Formamide amidohy...	38	0.038
31	NRL3_ARATH (P46010) Nitrilase 3 (EC 3.5.5.1)	38	0.038
32	AMIE_BACST (Q9RQ17) Aliphatic amidase (EC 3.5.1.4) (Acylamide am...	36	0.14
33	NRL1_ARATH (P32961) Nitrilase 1 (EC 3.5.5.1)	35	0.24
34	YAFV_ECOLI (Q47679) Hypothetical UPF0012 protein yafV (EC 3.5.-.-)	35	0.24
35	NRL2_ARATH (P32962) Nitrilase 2 (EC 3.5.5.1)	35	0.32
36	FREM1_MOUSE (Q684R7) FRAS1-related extracellular matrix protein ...	34	0.42
37	LNT_THEMA (Q9WZ43) Apolipoprotein N-acyltransferase (EC 2.3.1.-)...	33	1.2
38	NADE2_THEMA (Q9X0Y0) Probable glutamine-dependent NAD(+) synthet...	32	1.6
39	AMIE_BACSP (Q9L543) Aliphatic amidase (EC 3.5.1.4) (Acylamide am...	32	1.6
40	DPO3B_STRCO (P27903) DNA polymerase III beta subunit (EC 2.7.7.7)	32	1.6
41	AMIE_HELPY (O25067) Aliphatic amidase (EC 3.5.1.4) (Acylamide am...	32	2.7
42	AMIE_HELPJ (Q9ZME1) Aliphatic amidase (EC 3.5.1.4) (Acylamide am...	32	2.7
43	DPO3B_MYCTU (Q50790) DNA polymerase III beta subunit (EC 2.7.7.7)	32	2.7
44	DPO3B_MYCBO (O33914) DNA polymerase III beta subunit (EC 2.7.7.7)	32	2.7
45	ILV3_YEAST (P39522) Dihydroxy-acid dehydratase, mitochondrial pr...	31	4.6
46	RPOB_NEIG1 (Q5F5R5) DNA-directed RNA polymerase beta chain (EC 2...	30	6.0
47	YCZ2_SCHPO (O74556) Putative mannan endo-1,6-alpha-mannosidase C...	30	6.0
48	AMIE_RHOER (Q01360) Aliphatic amidase (EC 3.5.1.4) (Acylamide am...	30	6.0
49	YB54_METTM (Q50777) Hypothetical 16.1 kDa protein in MTR region ...	30	7.9

>NIT1_RAT (Q7TQ94) Nitrilase homolog 1 (EC 3.5.-.-)|
          Length = 292

 Score = 68.6 bits (166), Expect = 2e-11
 Identities = 42/142 (29%), Positives = 71/142 (50%)
 Frame = -3

Query: 707 KTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGH 528
           KT    +G+ IC+D  FPE +  +   GAEIL YP+A GS      +    HW+ +++  
Sbjct: 157 KTPAGKVGLAICYDMRFPELSLKLAQAGAEILTYPSAFGS------VTGPAHWEVLLRAR 210

Query: 527 AGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFD 348
           A  +   ++A+ + G+        + T   YG+S +  P G +V   ++    + +A  D
Sbjct: 211 AIESQCYVIAAAQCGRH-------HETRASYGHSMVVDPWGTVVASCSE-GPGLCLARID 262

Query: 347 LDEIKSTRHGWGIFRDRRPDLY 282
           L  ++  R    +F+ RRPDLY
Sbjct: 263 LHFLQQMRQHLPVFQHRRPDLY 284

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>NIT1_HUMAN (Q86X76) Nitrilase homolog 1 (EC 3.5.-.-)|
          Length = 327

 Score = 68.2 bits (165), Expect = 3e-11
 Identities = 42/141 (29%), Positives = 70/141 (49%)
 Frame = -3

Query: 704 TKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHA 525
           T    IG+ +C+D  FPE + A+   GAEIL YP+A GS      +    HW+ +++  A
Sbjct: 193 TPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGS------ITGPAHWEVLLRARA 246

Query: 524 GANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDL 345
                 +VA+ + G+        +     YG+S +  P G +V   ++    + +A  DL
Sbjct: 247 IETQCYVVAAAQCGRH-------HEKRASYGHSMVVDPWGTVVARCSE-GPGLCLARIDL 298

Query: 344 DEIKSTRHGWGIFRDRRPDLY 282
           + ++  R    +F+ RRPDLY
Sbjct: 299 NYLRQLRRHLPVFQHRRPDLY 319

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>DCAS_AGRSK (P60327) N-carbamoyl-D-amino acid hydrolase (EC 3.5.1.77)|
           (D-N-alpha-carbamilase)
          Length = 304

 Score = 67.4 bits (163), Expect = 4e-11
 Identities = 53/161 (32%), Positives = 75/161 (46%), Gaps = 5/161 (3%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILF----YPTAIGSEPQDMNLD 564
           GD GF  +    A +G+ IC D+ +PE  R M L+GAEI+      PT     PQ  +L 
Sbjct: 153 GDLGFPVYDVDAAKMGMFICNDRRWPEAWRVMGLRGAEIICGGYNTPTHNPPVPQHDHLT 212

Query: 563 SREHWKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLAN 384
           S  H    MQ  +  N     A+ ++G E              G+S I  PTGEIV L  
Sbjct: 213 SFHHLLS-MQAGSYQNGAWSAAAGKVGME--------ENCMLLGHSCIVAPTGEIVALTT 263

Query: 383 DKDEEVLVAEFDLDEIKSTR-HGWGIFRDRRPDLYKVLLTL 264
             ++EV+ A  DLD  +  R H +   + R+P  Y ++  L
Sbjct: 264 TLEDEVITAAVDLDRCRELREHIFNFKQHRQPQHYGLIAEL 304

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>DCAS_AGRTU (Q44185) N-carbamoyl-D-amino acid hydrolase (EC 3.5.1.77)|
           (D-N-alpha-carbamilase)
          Length = 304

 Score = 67.0 bits (162), Expect = 6e-11
 Identities = 53/158 (33%), Positives = 74/158 (46%), Gaps = 5/158 (3%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILF----YPTAIGSEPQDMNLD 564
           GD GF  +    A +G+ IC D+ +PET R M L+GAEI+      PT     PQ  +L 
Sbjct: 153 GDLGFPVYDVDAAKMGMFICNDRRWPETWRVMGLKGAEIICGGYNTPTHNPPVPQHDHLT 212

Query: 563 SREHWKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLAN 384
           S  H    MQ  +  N     A+ ++G E              G+S I  PTGEIV L  
Sbjct: 213 SFHHLLS-MQAGSYQNGAWSAAAGKVGME--------EGCMLLGHSCIVAPTGEIVALTT 263

Query: 383 DKDEEVLVAEFDLDEIKSTR-HGWGIFRDRRPDLYKVL 273
             ++EV+ A  DLD  +  R H +     R+P  Y ++
Sbjct: 264 TLEDEVITAAVDLDRCRELREHIFNFKAHRQPQHYGLI 301

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>NIT3_YEAST (P49954) Probable hydrolase NIT3 (EC 3.5.-.-)|
          Length = 291

 Score = 66.2 bits (160), Expect = 1e-10
 Identities = 44/141 (31%), Positives = 68/141 (48%)
 Frame = -3

Query: 704 TKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHA 525
           TKY   GVGIC+D  FPE A     +GA  + YP+A  +    +      HW  + +  A
Sbjct: 159 TKYGKFGVGICYDMRFPELAMLSARKGAFAMIYPSAFNTVTGPL------HWHLLARSRA 212

Query: 524 GANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDL 345
             N V ++  +          +  S+   YG+S +  P G+IV  A +  EE++ AE D 
Sbjct: 213 VDNQVYVMLCS-------PARNLQSSYHAYGHSIVVDPRGKIVAEAGE-GEEIIYAELDP 264

Query: 344 DEIKSTRHGWGIFRDRRPDLY 282
           + I+S R    + + RR D+Y
Sbjct: 265 EVIESFRQAVPLTKQRRFDVY 285

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>NIT1_MOUSE (Q8VDK1) Nitrilase homolog 1 (EC 3.5.-.-)|
          Length = 323

 Score = 65.5 bits (158), Expect = 2e-10
 Identities = 41/142 (28%), Positives = 70/142 (49%)
 Frame = -3

Query: 707 KTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGH 528
           KT    +G+ IC+D  FPE +  +   GAEIL Y +A GS      +    HW+ +++  
Sbjct: 188 KTPAGKVGLAICYDMRFPELSLKLAQAGAEILTYSSAFGS------VTGPAHWEVLLRAR 241

Query: 527 AGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFD 348
           A  +   ++A+ + G+        + T   YG+S +  P G +V   ++    + +A  D
Sbjct: 242 AIESQCYVIAAAQCGRH-------HETRASYGHSMVVDPWGTVVARCSE-GPGLCLARID 293

Query: 347 LDEIKSTRHGWGIFRDRRPDLY 282
           L  ++  R    +F+ RRPDLY
Sbjct: 294 LHFLQQMRQHLPVFQHRRPDLY 315

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>BUP1_PONPY (Q5RBM6) Beta-ureidopropionase (EC 3.5.1.6) (Beta-alanine synthase)|
           (N-carbamoyl-beta-alanine amidohydrolase)
          Length = 384

 Score = 59.3 bits (142), Expect = 1e-08
 Identities = 50/181 (27%), Positives = 79/181 (43%), Gaps = 9/181 (4%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTA-IGSEPQDMNLDSRE 555
           G+ G   F+T++  I V IC+ +  P       + GAEI+F P+A IG+  + +      
Sbjct: 214 GNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESL------ 267

Query: 554 HWKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIK--------FYGNSFIAGPTGEI 399
            W    +  A AN     A NR+G E    E  +   K        FYG+S++A P G  
Sbjct: 268 -WSIEARNAAIANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDGSR 326

Query: 398 VKLANDKDEEVLVAEFDLDEIKSTRHGWGIFRDRRPDLYKVLLTLDGKTSSSS*VPLFMK 219
               +   + +LVA+ DL+  +     W      R ++Y   L    K++ S   P  +K
Sbjct: 327 TPGLSRSQDGLLVAKLDLNLCQQVNDVWNFKMTGRYEMYARELAEAVKSNYS---PTIVK 383

Query: 218 E 216
           E
Sbjct: 384 E 384

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>BUP1_RAT (Q03248) Beta-ureidopropionase (EC 3.5.1.6) (Beta-alanine synthase)|
           (N-carbamoyl-beta-alanine amidohydrolase)
          Length = 393

 Score = 57.0 bits (136), Expect = 6e-08
 Identities = 50/183 (27%), Positives = 79/183 (43%), Gaps = 9/183 (4%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTA-IGSEPQDMNLDSRE 555
           G+ G   F+T++  I V IC+ +  P       + GAEI+F P+A IG   + M      
Sbjct: 214 GNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSVNGAEIIFNPSATIGELSESM------ 267

Query: 554 HWKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIK--------FYGNSFIAGPTGEI 399
            W    +  A AN     A NR+G+E    E  +   K        FYG+S++A P G  
Sbjct: 268 -WPIEARNAAIANHCFTCALNRVGQEHYPNEFTSGDGKKAHHDLGYFYGSSYVAAPDGSR 326

Query: 398 VKLANDKDEEVLVAEFDLDEIKSTRHGWGIFRDRRPDLYKVLLTLDGKTSSSS*VPLFMK 219
               +   + +LV E +L+  +     W      R ++Y   L    K + S   P  +K
Sbjct: 327 TPGLSRNQDGLLVTELNLNLCQQINDFWTFKMTGRLEMYARELAEAVKPNYS---PNIVK 383

Query: 218 EEM 210
           E++
Sbjct: 384 EDL 386

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>BUP1_MOUSE (Q8VC97) Beta-ureidopropionase (EC 3.5.1.6) (Beta-alanine synthase)|
           (N-carbamoyl-beta-alanine amidohydrolase)
          Length = 393

 Score = 56.6 bits (135), Expect = 8e-08
 Identities = 49/182 (26%), Positives = 77/182 (42%), Gaps = 8/182 (4%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREH 552
           G+ G   F+T++  I V IC+ +  P       + GAEI+F P+A   E       S   
Sbjct: 214 GNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGEL------SESL 267

Query: 551 WKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIK--------FYGNSFIAGPTGEIV 396
           W    +  A AN     A NR+G+E    E  +   K        FYG+S++A P G   
Sbjct: 268 WPIEARNAAIANHCFTCALNRVGQEHFPNEFTSGDGKKAHHDLGYFYGSSYVAAPDGSRT 327

Query: 395 KLANDKDEEVLVAEFDLDEIKSTRHGWGIFRDRRPDLYKVLLTLDGKTSSSS*VPLFMKE 216
              +   + +LV E +L+  +     W      R ++Y   L    K + S   P  +KE
Sbjct: 328 PGLSRNQDGLLVTELNLNLCQQINDFWTFKMTGRLEMYARELAEAVKPNYS---PNIVKE 384

Query: 215 EM 210
           ++
Sbjct: 385 DL 386

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>YAG5_STAAU (P55177) Hypothetical UPF0012 protein in agr operon (EC 3.5.-.-)|
           (ORF 5)
          Length = 261

 Score = 55.8 bits (133), Expect = 1e-07
 Identities = 36/133 (27%), Positives = 67/133 (50%)
 Frame = -3

Query: 677 ICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLVPLVA 498
           IC+D  FPE  R     GA+I FY   +   P    +   +HW  +++  A  N + ++ 
Sbjct: 145 ICYDLRFPELLRYPARSGAKIAFY---VAQWP----MSRLQHWHSLLKARAIENNMFVIG 197

Query: 497 SNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEIKSTRHG 318
           +N  G +      GN+  ++ G+S +  P G++V   N+   ++L  + +L+E++  R  
Sbjct: 198 TNSTGFD------GNT--EYAGHSIVINPNGDLVGELNE-SADILTVDLNLNEVEQQREN 248

Query: 317 WGIFRDRRPDLYK 279
             +F+  + DLYK
Sbjct: 249 IPVFKSIKLDLYK 261

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>NFT1_CAEEL (O76463) Nitrilase and fragile histidine triad fusion protein|
           NitFhit [Includes: Bis(5'-adenosyl)-triphosphatase (EC
           3.6.1.29) (Diadenosine 5',5'''-P1,P3-triphosphate
           hydrolase) (Dinucleosidetriphosphatase) (AP3A hydrolase)
           (AP3Aase); Nitrilas
          Length = 440

 Score = 55.8 bits (133), Expect = 1e-07
 Identities = 38/141 (26%), Positives = 66/141 (46%)
 Frame = -3

Query: 704 TKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHA 525
           T    +G+ IC+D  FPE +     +GA++L +P+A              HW+ +++  A
Sbjct: 159 TPIGRLGLSICYDVRFPELSLWNRKRGAQLLSFPSAFTLN------TGLAHWETLLRARA 212

Query: 524 GANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDL 345
             N   +VA+ + G         N   + YG+S +  P G +V   +++  ++  AE DL
Sbjct: 213 IENQCYVVAAAQTGAH-------NPKRQSYGHSMVVDPWGAVVAQCSER-VDMCFAEIDL 264

Query: 344 DEIKSTRHGWGIFRDRRPDLY 282
             + + R    +F  RR DLY
Sbjct: 265 SYVDTLREMQPVFSHRRSDLY 285

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>BUP1_HUMAN (Q9UBR1) Beta-ureidopropionase (EC 3.5.1.6) (Beta-alanine synthase)|
           (N-carbamoyl-beta-alanine amidohydrolase) (BUP-1)
          Length = 384

 Score = 55.8 bits (133), Expect = 1e-07
 Identities = 49/181 (27%), Positives = 78/181 (43%), Gaps = 9/181 (4%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTA-IGSEPQDMNLDSRE 555
           G+ G   F+T++  I V IC+ +  P       + GAEI+F P+A IG+  + +      
Sbjct: 214 GNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESL------ 267

Query: 554 HWKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIK--------FYGNSFIAGPTGEI 399
            W    +  A AN     A NR+G E    E  +   K        FYG+S++A P    
Sbjct: 268 -WPIEARNAAIANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDSSR 326

Query: 398 VKLANDKDEEVLVAEFDLDEIKSTRHGWGIFRDRRPDLYKVLLTLDGKTSSSS*VPLFMK 219
               +   + +LVA+ DL+  +     W      R ++Y   L    K++ S   P  +K
Sbjct: 327 TPGLSRSRDGLLVAKLDLNLCQQVNDVWNFKMTGRYEMYARELAEAVKSNYS---PTIVK 383

Query: 218 E 216
           E
Sbjct: 384 E 384

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>YPQQ_PSEFL (P55176) Hypothetical UPF0012 protein in pqqF 5'region (EC 3.5.-.-)|
           (ORF2)
          Length = 285

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 45/150 (30%), Positives = 65/150 (43%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREH 552
           G+  F   +     +G  IC+D  FPE AR + L GAE++  PTA    P D   D    
Sbjct: 146 GEDDFPLVELDGWKLGFLICYDIEFPENARRLALAGAELILVPTA-NMIPYDFVADV--- 201

Query: 551 WKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDE 372
               ++  A  N   +  +N  G E          I++ G S IA P G  + LA   DE
Sbjct: 202 ---TIRARAFENQCYVAYANYCGHE--------EQIRYCGQSSIAAPDGSRIALAG-LDE 249

Query: 371 EVLVAEFDLDEIKSTRHGWGIFRDRRPDLY 282
            +++   D   +  +R       DRRP+LY
Sbjct: 250 ALIIGTLDRQLMGESRALNRYLSDRRPELY 279

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>NFT1_DROME (O76464) Nitrilase and fragile histidine triad fusion protein|
           NitFhit (NFT-1 protein) [Includes:
           Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
           (Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
           (Dinucleosidetriphosphatase) (AP3A hydrolase) (AP
          Length = 460

 Score = 53.9 bits (128), Expect = 5e-07
 Identities = 38/141 (26%), Positives = 65/141 (46%)
 Frame = -3

Query: 704 TKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHA 525
           T    IG+ IC+D  F E A  +   GA +L YP+A            + HW+ +++  A
Sbjct: 173 TPVGQIGLQICYDLRFAEPAVLLRKLGANLLTYPSAF------TYATGKAHWEILLRARA 226

Query: 524 GANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDL 345
                 +VA+ +IG         N   + +G+S I  P G ++   ++++ ++  AE DL
Sbjct: 227 IETQCFVVAAAQIGWH-------NQKRQSWGHSMIVSPWGNVLADCSEQELDIGTAEVDL 279

Query: 344 DEIKSTRHGWGIFRDRRPDLY 282
             ++S       F  RR D+Y
Sbjct: 280 SVLQSLYQTMPCFEHRRNDIY 300

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>YAUB_SCHPO (Q10166) Hypothetical UPF0012 protein C26A3.11 in chromosome I (EC|
           3.5.-.-)
          Length = 322

 Score = 53.1 bits (126), Expect = 9e-07
 Identities = 36/145 (24%), Positives = 66/145 (45%)
 Frame = -3

Query: 704 TKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHA 525
           T+Y   G+GIC+D  FPE A      G  ++ YP A       +      HW+ + +  A
Sbjct: 185 TEYGKFGLGICYDIRFPELAMIAARNGCSVMIYPGAFNLSTGPL------HWELLARARA 238

Query: 524 GANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDL 345
             N +  VA     ++       N+    +G+S +  P G+++   ++K   ++ A+ D 
Sbjct: 239 VDNEM-FVACCAPARDM------NADYHSWGHSTVVDPFGKVIATTDEK-PSIVYADIDP 290

Query: 344 DEIKSTRHGWGIFRDRRPDLYKVLL 270
             + + R+   I+  RR D+Y  +L
Sbjct: 291 SVMSTARNSVPIYTQRRFDVYSEVL 315

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>Y601_SYNY3 (P55175) Hypothetical UPF0012 protein sll0601 (EC 3.5.-.-)|
          Length = 272

 Score = 53.1 bits (126), Expect = 9e-07
 Identities = 36/125 (28%), Positives = 61/125 (48%)
 Frame = -3

Query: 698 YATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGA 519
           +  +G+ IC+D  FPE  R +  QGA++LF P A  +         ++HW+ ++Q  A  
Sbjct: 150 FGNLGLSICYDVRFPELYRYLSRQGADVLFVPAAFTA------YTGKDHWQVLLQARAIE 203

Query: 518 NLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDE 339
           N   ++A  + G    E  H       +G++ I  P G I+  A +K   + +AE + D 
Sbjct: 204 NTCYVIAPAQTGCH-YERRH------THGHAMIIDPWGVILADAGEK-PGLAIAEINPDR 255

Query: 338 IKSTR 324
           +K  R
Sbjct: 256 LKQVR 260

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>YHCX_BACSU (P54608) Hypothetical UPF0012 protein yhcX (EC 3.5.-.-)|
          Length = 513

 Score = 48.9 bits (115), Expect = 2e-05
 Identities = 44/156 (28%), Positives = 70/156 (44%), Gaps = 9/156 (5%)
 Frame = -3

Query: 716 KAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRV- 540
           + F T    I + IC+D  FPE AR    +GA+I+F P            + R+ + RV 
Sbjct: 365 RVFDTDCGKIAIQICYDIEFPELARIAADKGAKIIFTPFC---------TEDRQGYLRVR 415

Query: 539 --MQGHAGANLVPLVASNRIGK----ETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDK 378
              Q  A  N +  V S  +G     E ++ ++  S I F  + F     G IV   N  
Sbjct: 416 YCSQARAVENQIYTVISGTVGNLPQTENMDIQYAQSGI-FAPSDFEFARDG-IVGETNPN 473

Query: 377 DEEVLVAEFDLDEIKSTRHGWGI--FRDRRPDLYKV 276
            E V++ + DL+ ++  R    +   +DRR D+Y +
Sbjct: 474 IEMVVIGDVDLEILRRQRQNGTVRQLKDRRRDIYHI 509

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>YAG5_STALU (P55178) Hypothetical UPF0012 protein in agr operon (ORF 5)|
           (Fragment)
          Length = 234

 Score = 47.8 bits (112), Expect = 4e-05
 Identities = 35/133 (26%), Positives = 57/133 (42%)
 Frame = -3

Query: 677 ICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLVPLVA 498
           IC+D  FPE  R     GA I FY     S           HW+ +++  A  N + ++ 
Sbjct: 118 ICYDLRFPELLRYPARSGATIAFYVAQWPSA-------RLNHWQVLLKARAIENNMYVIG 170

Query: 497 SNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEIKSTRHG 318
            N  G +           ++ G+S    P GEI++  +  ++E+ V   D+D ++  R  
Sbjct: 171 CNGCGYD--------GKTQYAGHSVAINPNGEIIQELSTTEKELTVT-IDIDAVEQQRKA 221

Query: 317 WGIFRDRRPDLYK 279
             +F    P LYK
Sbjct: 222 IPVFDSLVPHLYK 234

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>YBEM_ECOLI (P39874) Hypothetical UPF0012 protein ybeM (EC 3.5.-.-)|
          Length = 262

 Score = 46.2 bits (108), Expect = 1e-04
 Identities = 35/132 (26%), Positives = 55/132 (41%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLV 510
           +G+  C+D  FPE A A  LQGAEIL  P A    P         HW  ++   A     
Sbjct: 142 VGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPL-----KEHHWSTLLAARALDTTC 196

Query: 509 PLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEIKS 330
            +VA+   G + +            G S I  P G  +  A++    +++AE   + ++ 
Sbjct: 197 YMVAAGECGNKNI------------GQSRIIDPFGVTIAAASEM-PALIMAEVTPERVRQ 243

Query: 329 TRHGWGIFRDRR 294
            R    +  +RR
Sbjct: 244 VRAQLPVLNNRR 255

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>YBEM_ECO57 (P58054) Hypothetical UPF0012 protein ybeM (EC 3.5.-.-)|
          Length = 262

 Score = 46.2 bits (108), Expect = 1e-04
 Identities = 35/132 (26%), Positives = 55/132 (41%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLV 510
           +G+  C+D  FPE A A  LQGAEIL  P A    P         HW  ++   A     
Sbjct: 142 VGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPL-----KEHHWSTLLAARALDTTC 196

Query: 509 PLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEIKS 330
            +VA+   G + +            G S I  P G  +  A++    +++AE   + ++ 
Sbjct: 197 YMVAAGECGNKNI------------GQSRIIDPFGVTIAAASEM-PALIMAEVTPERVRQ 243

Query: 329 TRHGWGIFRDRR 294
            R    +  +RR
Sbjct: 244 VRAQLPVLNNRR 255

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>Y480_MYCTU (Q11146) Hypothetical UPF0012 protein Rv0480c/MT0498 (EC 3.5.-.-)|
          Length = 340

 Score = 45.8 bits (107), Expect = 1e-04
 Identities = 32/133 (24%), Positives = 63/133 (47%), Gaps = 2/133 (1%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLV 510
           +G+ +C+D  FP     +  +GA+++    + GS P  +     E W  + +  A  ++ 
Sbjct: 201 VGLTVCYDIRFPALYTELARRGAQLIAVCASWGSGPGKL-----EQWTLLARARALDSMS 255

Query: 509 PLVASNRIGKETVETEHGNSTI--KFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEI 336
            + A+ +       T  G S+      G S +A P GE+V  A  +  ++LVA+ D+D +
Sbjct: 256 YVAAAGQADPGDARTGVGASSAAPTGVGGSLVASPLGEVVVSAGTQ-PQLLVADIDVDNV 314

Query: 335 KSTRHGWGIFRDR 297
            + R    + R++
Sbjct: 315 AAARDRIAVLRNQ 327

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>AMIF_BRAJA (Q89H51) Formamidase (EC 3.5.1.49) (Formamide amidohydrolase)|
          Length = 337

 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 41/143 (28%), Positives = 61/143 (42%), Gaps = 2/143 (1%)
 Frame = -3

Query: 731 GDTGFKAFK-TKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSRE 555
           GD G    +  K A I + IC D  FPE AR    +GAEI+   TA  + P       RE
Sbjct: 142 GDIGIPVIEGPKGARIALIICHDGMFPEMARECAYKGAEIMI-RTAGYTAP------IRE 194

Query: 554 HWKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIA-GPTGEIVKLANDK 378
            W+   Q ++  NL+        G +      G   I  +  + +A G TG        +
Sbjct: 195 AWRFTNQANSFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGAVLAHGTTG--------R 246

Query: 377 DEEVLVAEFDLDEIKSTRHGWGI 309
            +E++ AE   D ++  R  WG+
Sbjct: 247 ADEIITAEVRPDLVREARINWGV 269

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>NADE_RHOCA (Q03638) Glutamine-dependent NAD(+) synthetase (EC 6.3.5.1) (NAD(+)|
           synthase [glutamine-hydrolyzing])
          Length = 552

 Score = 43.9 bits (102), Expect = 5e-04
 Identities = 30/97 (30%), Positives = 43/97 (44%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLV 510
           +GV +C D W P+ A A+   GAE+L  P   GS  +   LD R   ++V         +
Sbjct: 144 LGVPVCEDAWHPDVAGALAAAGAEVLMVPN--GSPYRRGKLDLR---RQVTGARVAETGL 198

Query: 509 PLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEI 399
           PL+  N +G        G     F G SF+  P G +
Sbjct: 199 PLLYLNMVG--------GQDDQLFDGASFVLNPDGSV 227

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>NRL4_ARATH (P46011) Nitrilase 4 (EC 3.5.5.1)|
          Length = 355

 Score = 43.5 bits (101), Expect = 7e-04
 Identities = 38/158 (24%), Positives = 62/158 (39%), Gaps = 13/158 (8%)
 Frame = -3

Query: 710 FKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQG 531
           F T    IG  ICW+   P    AM  +G EI   PTA          DSRE W   M  
Sbjct: 185 FDTPIGKIGAAICWENRMPSLRTAMYAKGIEIYCAPTA----------DSRETWLASMTH 234

Query: 530 HAGANLVPLVASNRIGKET------------VETEHGNSTIKFYGNSFIAGPTGEIVKLA 387
            A      ++++N+  +               E      ++   G S I  P G ++   
Sbjct: 235 IALEGGCFVLSANQFCRRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGP 294

Query: 386 NDKDEEVLVAEFDLDEIKSTRHGWGIF-RDRRPDLYKV 276
           N + E ++ A+ DL +I   +  + +     RP+++ +
Sbjct: 295 NYRGEALITADLDLGDIARAKFDFDVVGHYSRPEVFSL 332

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>NIT2_YEAST (P47016) Probable hydrolase NIT2 (EC 3.5.-.-)|
          Length = 307

 Score = 43.1 bits (100), Expect = 9e-04
 Identities = 32/149 (21%), Positives = 68/149 (45%), Gaps = 13/149 (8%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLV 510
           +G  IC+D  FPE +  +   GAEIL +P+A   +          HW+ + +  A     
Sbjct: 164 LGSAICYDIRFPEFSLKLRSMGAEILCFPSAFTIK------TGEAHWELLGRARAVDTQC 217

Query: 509 PLVASNRIG-----------KETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDK--DEE 369
            ++   ++G           +  +     +S  + +G+S +  P G+I+  A+      +
Sbjct: 218 YVLMPGQVGMHDLSDPEWEKQSHMSALEKSSRRESWGHSMVIDPWGKIIAHADPSTVGPQ 277

Query: 368 VLVAEFDLDEIKSTRHGWGIFRDRRPDLY 282
           +++A+ D + ++  R+   ++  RR DL+
Sbjct: 278 LILADLDRELLQEIRNKMPLWNQRRDDLF 306

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>NRL4_TOBAC (Q42965) Nitrilase 4 (EC 3.5.5.1)|
          Length = 349

 Score = 43.1 bits (100), Expect = 9e-04
 Identities = 38/160 (23%), Positives = 65/160 (40%), Gaps = 13/160 (8%)
 Frame = -3

Query: 710 FKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQG 531
           + T    IG  ICW+   P    AM  +G EI   PTA          DSR+ W+  M  
Sbjct: 178 YDTPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTA----------DSRDVWQASMTH 227

Query: 530 HAGANLVPLVASNRIGKET------------VETEHGNSTIKFYGNSFIAGPTGEIVKLA 387
            A      ++++N+  +               E +    +I   G S I  P+G ++   
Sbjct: 228 IALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGP 287

Query: 386 NDKDEEVLVAEFDLDEIKSTRHGWGIF-RDRRPDLYKVLL 270
           N   E ++ A+ DL EI   +  + +     RP++  +++
Sbjct: 288 NYVGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLIV 327

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>NRL_BACSX (P82605) Nitrilase (EC 3.5.5.1)|
          Length = 338

 Score = 41.6 bits (96), Expect = 0.003
 Identities = 38/133 (28%), Positives = 64/133 (48%), Gaps = 8/133 (6%)
 Frame = -3

Query: 710 FKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQG 531
           F+T++  +G   CW+ + P    AM     ++      IG  PQ+ +L   E      + 
Sbjct: 149 FETEFGNLGGLQCWEHFLPLNVAAMASMNEQVHVASWPIGM-PQEGHLFGPEQCVTATKY 207

Query: 530 HAGANLVPLVASNRIGKE-----TVETEHGNSTIKF-YGNSFIAGPTGEIV--KLANDKD 375
           +A +N V  + S++I  E       ETE   + +K  +G S I  P G  +  KLA+D +
Sbjct: 208 YAISNQVFCLLSSQIWTEEQRDKICETEEQRNFMKVGHGFSKIIAPNGMEIGNKLAHD-E 266

Query: 374 EEVLVAEFDLDEI 336
           E +  A+ DL++I
Sbjct: 267 EGITYADIDLEQI 279

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>AMIF_PSESM (Q887D9) Formamidase (EC 3.5.1.49) (Formamide amidohydrolase)|
          Length = 338

 Score = 41.6 bits (96), Expect = 0.003
 Identities = 33/129 (25%), Positives = 53/129 (41%)
 Frame = -3

Query: 695 ATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGAN 516
           +T+ + IC D  FPE AR    +GA+I+   TA  + P       R  WK   Q +A  N
Sbjct: 156 STLALIICHDGMFPEMARECAYKGADIML-RTAGYTAP------IRHSWKITNQSNAFTN 208

Query: 515 LVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEI 336
           L+   +    G +         T    G +      G I+     + +E++  E   D +
Sbjct: 209 LMQTASVCMCGSD--------GTFDSMGEAMFVDFDGTIMAEGGGRADEIVCCELRPDLV 260

Query: 335 KSTRHGWGI 309
           +  R  WG+
Sbjct: 261 REARVHWGV 269

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>AMIF_BACCR (P59701) Formamidase (EC 3.5.1.49) (Formamide amidohydrolase)|
          Length = 332

 Score = 37.7 bits (86), Expect = 0.038
 Identities = 29/127 (22%), Positives = 50/127 (39%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLV 510
           + V IC D  FPE AR    +GA +L   +   ++         E W    + +A  NL+
Sbjct: 160 LAVCICHDGMFPEVAREAAYKGANVLIRISGYSTQVS-------EQWMLTNRSNAWQNLM 212

Query: 509 PLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEIKS 330
             ++ N  G + V          ++G   +    G  +   +    E++ AE   +    
Sbjct: 213 YTLSVNLAGYDGV--------FYYFGEGQVCNFDGTTLVQGHRNPWEIVTAEVYPELADQ 264

Query: 329 TRHGWGI 309
            R GWG+
Sbjct: 265 ARLGWGL 271

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>AMIF_BACAN (P59700) Formamidase (EC 3.5.1.49) (Formamide amidohydrolase)|
          Length = 332

 Score = 37.7 bits (86), Expect = 0.038
 Identities = 29/127 (22%), Positives = 50/127 (39%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLV 510
           + V IC D  FPE AR    +GA +L   +   ++         E W    + +A  NL+
Sbjct: 160 LAVCICHDGMFPEVAREAAYKGANVLIRISGYSTQVS-------EQWMLTNRSNAWQNLM 212

Query: 509 PLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEIKS 330
             ++ N  G + V          ++G   +    G  +   +    E++ AE   +    
Sbjct: 213 YTLSVNLAGYDGV--------FYYFGEGQVCNFDGTTLVQGHRNPWEIVTAEVYPELADQ 264

Query: 329 TRHGWGI 309
            R GWG+
Sbjct: 265 ARLGWGL 271

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>NRL3_ARATH (P46010) Nitrilase 3 (EC 3.5.5.1)|
          Length = 346

 Score = 37.7 bits (86), Expect = 0.038
 Identities = 36/159 (22%), Positives = 69/159 (43%), Gaps = 14/159 (8%)
 Frame = -3

Query: 710 FKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQG 531
           + T    IG  ICW+   P    A+  +G EI   PTA      D +L+    W+  M  
Sbjct: 174 YDTPIGKIGAAICWENRMPLYRTALYAKGIEIYCAPTA------DYSLE----WQASMIH 223

Query: 530 HAGANLVPLVASNRIGK-------------ETVETEHGNSTIKFYGNSFIAGPTGEIVKL 390
            A      ++++++  K             + V+T+  + T+   G S I  P G+++  
Sbjct: 224 IAVEGGCFVLSAHQFCKRREFPEHPDYLFNDIVDTKEHDPTVS-GGGSVIISPLGKVLAG 282

Query: 389 ANDKDEEVLVAEFDLDEIKSTRHGWGIF-RDRRPDLYKV 276
            N + E ++ A+ DL +I   +  + +     +PD++ +
Sbjct: 283 PNYESEGLVTADLDLGDIARAKLYFDVVGHYSKPDIFNL 321

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>AMIE_BACST (Q9RQ17) Aliphatic amidase (EC 3.5.1.4) (Acylamide amidohydrolase)|
           (Wide spectrum amidase)
          Length = 348

 Score = 35.8 bits (81), Expect = 0.14
 Identities = 33/136 (24%), Positives = 57/136 (41%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREH 552
           GDT +     K   I + IC D  +PE  R   ++GAE++          Q     ++E 
Sbjct: 147 GDTTYVTEGPKGIKISLIICDDGNYPEIWRDCAMKGAELIV-------RCQGYMYPAKEQ 199

Query: 551 WKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDE 372
              + +  A AN V +  +N  G + V          ++G+S I G  G  +    +++ 
Sbjct: 200 QIMMAKTMAWANNVYVAVANATGFDGV--------YSYFGHSAIIGFDGRTLGECGEEEN 251

Query: 371 EVLVAEFDLDEIKSTR 324
            +  AE  L +I+  R
Sbjct: 252 GIQYAEISLSQIRDFR 267

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>NRL1_ARATH (P32961) Nitrilase 1 (EC 3.5.5.1)|
          Length = 346

 Score = 35.0 bits (79), Expect = 0.24
 Identities = 30/137 (21%), Positives = 55/137 (40%), Gaps = 12/137 (8%)
 Frame = -3

Query: 710 FKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQG 531
           + T    +G  ICW+   P    A+  +G E+   PTA GS          + W+  M  
Sbjct: 174 YDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGS----------KEWQSSMLH 223

Query: 530 HAGANLVPLVASNRIGKETVETEH------------GNSTIKFYGNSFIAGPTGEIVKLA 387
            A      ++++ +  +     +H             + +I   G S I  P G+++   
Sbjct: 224 IAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSIVSQGGSVIISPLGQVLAGP 283

Query: 386 NDKDEEVLVAEFDLDEI 336
           N + E ++ A+ DL +I
Sbjct: 284 NFESEGLVTADIDLGDI 300

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>YAFV_ECOLI (Q47679) Hypothetical UPF0012 protein yafV (EC 3.5.-.-)|
          Length = 256

 Score = 35.0 bits (79), Expect = 0.24
 Identities = 29/126 (23%), Positives = 55/126 (43%)
 Frame = -3

Query: 677 ICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLVPLVA 498
           +C+D  FP  +R   L   ++  Y   + + P   +L    HW+ ++   A  N   +  
Sbjct: 140 VCYDLRFPVWSRN--LNDYDLALY---VANWPAPRSL----HWQALLTARAIENQAYVAG 190

Query: 497 SNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEIKSTRHG 318
            NR+G +      GN    + G+S +  P GEI+  A+      + AE  +  ++  R  
Sbjct: 191 CNRVGSD------GNGC-HYRGDSRVINPQGEIIATADAHQATRIDAELSMAALREYREK 243

Query: 317 WGIFRD 300
           +  ++D
Sbjct: 244 FPAWQD 249

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>NRL2_ARATH (P32962) Nitrilase 2 (EC 3.5.5.1)|
          Length = 339

 Score = 34.7 bits (78), Expect = 0.32
 Identities = 31/137 (22%), Positives = 53/137 (38%), Gaps = 12/137 (8%)
 Frame = -3

Query: 710 FKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQG 531
           + T    +G  ICW+   P    A+  +G E+   PTA GS          + W+  M  
Sbjct: 167 YDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGS----------KEWQSSMLH 216

Query: 530 HA--GANLVPLVASNRIGKETVE----------TEHGNSTIKFYGNSFIAGPTGEIVKLA 387
            A  G   V       + K+  +           +    +I   G S I  P G+++   
Sbjct: 217 IAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAGP 276

Query: 386 NDKDEEVLVAEFDLDEI 336
           N + E ++ A+ DL ++
Sbjct: 277 NFESEGLITADLDLGDV 293

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>FREM1_MOUSE (Q684R7) FRAS1-related extracellular matrix protein 1 precursor|
            (QBRICK protein)
          Length = 2191

 Score = 34.3 bits (77), Expect = 0.42
 Identities = 33/131 (25%), Positives = 57/131 (43%), Gaps = 4/131 (3%)
 Frame = -3

Query: 707  KTKYATIGVGICWDQWFPETARAMVLQGAEILFYPT---AIGSEPQDMNLDSREHWKRVM 537
            ++K++T G G  W    P  + ++   G+ +L  P      G   Q   L      K + 
Sbjct: 1881 QSKHSTWGKGP-WHP-LPSGSSSLTTSGSPLLERPPPSFTSGDALQGFGLTDLTQRKTMT 1938

Query: 536  QGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVA 357
            QG+  + L   V  N  G +T+   HG  ++K  G+ F A      + + +     + VA
Sbjct: 1939 QGNGKSVLPSSVCRN--GTDTIYNYHGIVSLKLEGDRFSAHKRKAKISIVSQPQRTIKVA 1996

Query: 356  EFDL-DEIKST 327
            E  L D+++ST
Sbjct: 1997 ELPLADKVEST 2007

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>LNT_THEMA (Q9WZ43) Apolipoprotein N-acyltransferase (EC 2.3.1.-) (ALP|
           N-acyltransferase)
          Length = 503

 Score = 32.7 bits (73), Expect = 1.2
 Identities = 14/33 (42%), Positives = 21/33 (63%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIG 591
           + V IC++ +FPE +RA V  G+E+L   T  G
Sbjct: 366 LSVQICFESYFPEVSRAFVKNGSELLIVVTNDG 398

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>NADE2_THEMA (Q9X0Y0) Probable glutamine-dependent NAD(+) synthetase (EC|
           6.3.5.1) (NAD(+) synthase [glutamine-hydrolyzing])
          Length = 576

 Score = 32.3 bits (72), Expect = 1.6
 Identities = 27/117 (23%), Positives = 45/117 (38%)
 Frame = -3

Query: 689 IGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREHWKRVMQGHAGANLV 510
           +GV IC D W P    A +  G  +      + + P  +        K  +   A    V
Sbjct: 143 VGVTICEDIWNPVEPSASLSLGEGVHLIAN-LSASPYHVGKPVLR--KDYLSMKAYDYHV 199

Query: 509 PLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDE 339
            +   N +G        G   + F G S +   +GE++      +EE++  + DLDE
Sbjct: 200 AMAYCNMVG--------GQDELVFDGGSMVVDASGEVINYGKLFEEEIITVDLDLDE 248

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>AMIE_BACSP (Q9L543) Aliphatic amidase (EC 3.5.1.4) (Acylamide amidohydrolase)|
          Length = 348

 Score = 32.3 bits (72), Expect = 1.6
 Identities = 30/136 (22%), Positives = 55/136 (40%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREH 552
           GDT +     K   I + +C D  +PE  R   ++GAE++          Q     ++E 
Sbjct: 147 GDTTYVTEGPKGLKISLIVCDDGNYPEIWRDCAMKGAELIV-------RCQGYMYPAKEQ 199

Query: 551 WKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDE 372
              + +  A AN   +  +N  G + V          ++G+S I G  G  +     ++ 
Sbjct: 200 QIMMAKAMAWANNTYVAVANATGFDGV--------YSYFGHSAIIGFDGRTLGECGTEEN 251

Query: 371 EVLVAEFDLDEIKSTR 324
            +  AE  + +I+  R
Sbjct: 252 GIQYAEVSISQIRDFR 267

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>DPO3B_STRCO (P27903) DNA polymerase III beta subunit (EC 2.7.7.7)|
          Length = 376

 Score = 32.3 bits (72), Expect = 1.6
 Identities = 24/82 (29%), Positives = 38/82 (46%), Gaps = 1/82 (1%)
 Frame = +3

Query: 429 VPVELYGAVAML-SLDGFLSYPVRSNKGNQVSAGMALHDTLPVFTGIQVHILGFRTNSSG 605
           +PVE Y A+  +    G +   V ++   QV+      DTLPV TG+++ I G     + 
Sbjct: 109 LPVEEYPALPQMPEATGTVPGEVFASAVQQVAIAAGRDDTLPVLTGVRIEIEGDSVTLAS 168

Query: 606 IEQYFRPL*HHSPCSLWKPLIP 671
            ++Y   +       LWKP  P
Sbjct: 169 TDRYRFAVREF----LWKPENP 186

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>AMIE_HELPY (O25067) Aliphatic amidase (EC 3.5.1.4) (Acylamide amidohydrolase)|
          Length = 339

 Score = 31.6 bits (70), Expect = 2.7
 Identities = 28/136 (20%), Positives = 56/136 (41%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREH 552
           GD  +     K   + + IC D  +PE  R   ++GAE++          Q     ++E 
Sbjct: 146 GDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMRGAELIV-------RCQGYMYPAKEQ 198

Query: 551 WKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDE 372
              +++  A AN   +  +N  G + V          ++G+S I G  G  +    +++ 
Sbjct: 199 QIAIVKAMAWANQCYVAVANATGFDGV--------YSYFGHSSIIGFDGHTLGECGEEEN 250

Query: 371 EVLVAEFDLDEIKSTR 324
            +  A+  + +I+  R
Sbjct: 251 GLQYAQLSVQQIRDAR 266

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>AMIE_HELPJ (Q9ZME1) Aliphatic amidase (EC 3.5.1.4) (Acylamide amidohydrolase)|
          Length = 339

 Score = 31.6 bits (70), Expect = 2.7
 Identities = 28/136 (20%), Positives = 56/136 (41%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREH 552
           GD  +     K   + + IC D  +PE  R   ++GAE++          Q     ++E 
Sbjct: 146 GDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMRGAELIV-------RCQGYMYPAKEQ 198

Query: 551 WKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDE 372
              +++  A AN   +  +N  G + V          ++G+S I G  G  +    +++ 
Sbjct: 199 QIAIVKAMAWANQCYVAVANATGFDGV--------YSYFGHSSIIGFDGHTLGECGEEEN 250

Query: 371 EVLVAEFDLDEIKSTR 324
            +  A+  + +I+  R
Sbjct: 251 GLQYAQLSVQQIRDAR 266

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>DPO3B_MYCTU (Q50790) DNA polymerase III beta subunit (EC 2.7.7.7)|
          Length = 402

 Score = 31.6 bits (70), Expect = 2.7
 Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
 Frame = +3

Query: 429 VPVELYGAVAMLSLD-GFLSYPVRSNKGNQVSAGMALHDTLPVFTGIQVHILG 584
           +PVE Y  +  L  + G L   + +   +QV+      DTLP+ TGI+V ILG
Sbjct: 121 MPVEDYPTLPTLPEETGLLPAELFAEAISQVAIAAGRDDTLPMLTGIRVEILG 173

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>DPO3B_MYCBO (O33914) DNA polymerase III beta subunit (EC 2.7.7.7)|
          Length = 402

 Score = 31.6 bits (70), Expect = 2.7
 Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
 Frame = +3

Query: 429 VPVELYGAVAMLSLD-GFLSYPVRSNKGNQVSAGMALHDTLPVFTGIQVHILG 584
           +PVE Y  +  L  + G L   + +   +QV+      DTLP+ TGI+V ILG
Sbjct: 121 MPVEDYPTLPTLPEETGLLPAELFAEAISQVAIAAGRDDTLPMLTGIRVEILG 173

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>ILV3_YEAST (P39522) Dihydroxy-acid dehydratase, mitochondrial precursor (EC|
           4.2.1.9) (DAD) (2,3-dihydroxy acid hydrolyase)
          Length = 585

 Score = 30.8 bits (68), Expect = 4.6
 Identities = 34/141 (24%), Positives = 58/141 (41%), Gaps = 24/141 (17%)
 Frame = -3

Query: 725 TGFKAFKTKYATIGVGICWDQWFP----------ETARAMVLQGAEILFYPT-------A 597
           TGFK    K   +GVG CW    P            ++++   G + + + T       +
Sbjct: 46  TGFKKEDFKKPQVGVGSCWWSGNPCNMHLLDLNNRCSQSIEKAGLKAMQFNTIGVSDGIS 105

Query: 596 IGSEPQDMNLDSRE----HWKRVMQG-HAGANL-VPLVASNRIGKETVETEHGNSTIKFY 435
           +G++    +L SRE     ++ +M   H  AN+ +P    N  G       H   +I  Y
Sbjct: 106 MGTKGMRYSLQSREIIADSFETIMMAQHYDANIAIPSCDKNMPGVMMAMGRHNRPSIMVY 165

Query: 434 GNSFIAG-PTGEIVKLANDKD 375
           G + + G PT    K++ + D
Sbjct: 166 GGTILPGHPTCGSSKISKNID 186

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>RPOB_NEIG1 (Q5F5R5) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP|
           beta subunit) (Transcriptase beta chain) (RNA polymerase
           beta subunit)
          Length = 1392

 Score = 30.4 bits (67), Expect = 6.0
 Identities = 16/51 (31%), Positives = 27/51 (52%)
 Frame = -3

Query: 485 GKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDEEVLVAEFDLDEIK 333
           G   ++ E  +   K      I   TGE++  AND+  E L+A+FD++ +K
Sbjct: 286 GLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDINGVK 336

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>YCZ2_SCHPO (O74556) Putative mannan endo-1,6-alpha-mannosidase C970.02|
           precursor (EC 3.2.1.101) (Endo-alpha-1->6-D-mannanase
           C970.02)
          Length = 442

 Score = 30.4 bits (67), Expect = 6.0
 Identities = 18/70 (25%), Positives = 33/70 (47%)
 Frame = +1

Query: 70  GIFIDVIYWWEITNQVGNVLADRYLCRGKDMKIYRLSVWCSPSIVELISSFIKSGT*DDD 249
           G+F+   YWWE      N L +RY+  G            + +  EL+ + +   + +D 
Sbjct: 55  GMFLPPAYWWE-AGAAWNGLLNRYIATG------------NSTYNELVKTSMLYQSGEDS 101

Query: 250 DVLPSNVSST 279
           D +PSN +++
Sbjct: 102 DYMPSNYTTS 111

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>AMIE_RHOER (Q01360) Aliphatic amidase (EC 3.5.1.4) (Acylamide amidohydrolase)|
           (Wide spectrum amidase)
          Length = 345

 Score = 30.4 bits (67), Expect = 6.0
 Identities = 29/136 (21%), Positives = 55/136 (40%)
 Frame = -3

Query: 731 GDTGFKAFKTKYATIGVGICWDQWFPETARAMVLQGAEILFYPTAIGSEPQDMNLDSREH 552
           GDT +     K   I + IC D  +PE  R   ++GAE++          Q     +++ 
Sbjct: 147 GDTTYVTEGPKGLKISLIICDDGNYPEIWRDCAMKGAELIV-------RCQGYMYPAKDQ 199

Query: 551 WKRVMQGHAGANLVPLVASNRIGKETVETEHGNSTIKFYGNSFIAGPTGEIVKLANDKDE 372
              + +  A AN   +  +N  G + V          ++G+S I G  G  +    +++ 
Sbjct: 200 QVMMSKAMAWANNCYVAVANAAGFDGV--------YSYFGHSAIIGFDGRTLGETGEEEY 251

Query: 371 EVLVAEFDLDEIKSTR 324
            +  A+  +  I+  R
Sbjct: 252 GIQYAQLSVSAIRDAR 267

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>YB54_METTM (Q50777) Hypothetical 16.1 kDa protein in MTR region (ORF143)|
          Length = 143

 Score = 30.0 bits (66), Expect = 7.9
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = -3

Query: 455 NSTIKFYGNSFIAGPTGEIVKLANDKDEEVLV 360
           N  +KF G      P  EIVKLA ++D +V++
Sbjct: 78  NPNVKFRGVMLEGNPADEIVKLAEEEDVDVII 109


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109,413,249
Number of Sequences: 219361
Number of extensions: 2406729
Number of successful extensions: 6367
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 6179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6349
length of database: 80,573,946
effective HSP length: 109
effective length of database: 56,663,597
effective search space used: 7762912789
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

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full-length cDNA and EST

Clone Information

BLAST Search Result