ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbags31l16
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NECP1_MOUSE (Q9CR95) Adaptin ear-binding coat-associated protein... 41 0.004
2NECP1_RAT (P69682) Adaptin ear-binding coat-associated protein 1... 39 0.014
3NECP1_PONPY (Q5R630) Adaptin ear-binding coat-associated protein... 39 0.018
4NECP2_HUMAN (Q9NVZ3) Adaptin ear-binding coat-associated protein... 37 0.040
5NECP1_HUMAN (Q8NC96) Adaptin ear-binding coat-associated protein... 37 0.052
6NECP2_RAT (Q6P756) Adaptin ear-binding coat-associated protein 2... 37 0.068
7NECP2_MOUSE (Q9D1J1) Adaptin ear-binding coat-associated protein... 36 0.089
8SPB1_KLULA (Q6CV12) AdoMet-dependent rRNA methyltransferase SPB1... 31 2.9
9SN1L2_PONPY (Q5REX1) Serine/threonine-protein kinase SNF1-like k... 30 6.4
10ATG2_DEBHA (Q6BTX0) Autophagy-related protein 2 30 6.4
11PRE1_STAAU (P03857) Plasmid recombination enzyme type 1 (Plasmid... 30 8.3
12GLB_TETTH (Q03459) Myoglobin (Hemoglobin) 30 8.3

>NECP1_MOUSE (Q9CR95) Adaptin ear-binding coat-associated protein 1 (NECAP-1)|
          Length = 275

 Score = 40.8 bits (94), Expect = 0.004
 Identities = 22/73 (30%), Positives = 37/73 (50%), Gaps = 3/73 (4%)
 Frame = -2

Query: 659 EAYDFQAAXHDHMKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVLQIKN---KESGSK 489
           +A+DF  +  DH K++ ++    +  Q  +N   +D   KEG+T+ L I N   K+ G+ 
Sbjct: 115 DAFDFNVSLQDHFKWVKQETEISKESQEMDNRPKLDLGFKEGQTIKLSIGNITAKKGGAS 174

Query: 488 TKSAFFEQGLNKL 450
              A    GL+ L
Sbjct: 175 KPRASGTGGLSLL 187



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>NECP1_RAT (P69682) Adaptin ear-binding coat-associated protein 1 (NECAP-1)|
          Length = 277

 Score = 38.9 bits (89), Expect = 0.014
 Identities = 21/73 (28%), Positives = 37/73 (50%), Gaps = 3/73 (4%)
 Frame = -2

Query: 659 EAYDFQAAXHDHMKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVLQIKN---KESGSK 489
           +A+DF  +  DH K++ ++    +  Q  ++   +D   KEG+T+ L I N   K+ G+ 
Sbjct: 115 DAFDFNVSLQDHFKWVKQETEISKESQEMDSRPKLDLGFKEGQTIKLSIGNITAKKGGTS 174

Query: 488 TKSAFFEQGLNKL 450
              A    GL+ L
Sbjct: 175 KPRASGTGGLSLL 187



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>NECP1_PONPY (Q5R630) Adaptin ear-binding coat-associated protein 1 (NECAP-1)|
          Length = 275

 Score = 38.5 bits (88), Expect = 0.018
 Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
 Frame = -2

Query: 659 EAYDFQAAXHDHMKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVL---QIKNKESG-S 492
           +A+DF  +  DH K++ ++    +  Q  +    +D   KEG+T+ L    I NKE G S
Sbjct: 115 DAFDFNVSLQDHFKWVKQESEISKESQEMDARPKLDLGFKEGQTIKLSIGNITNKEGGAS 174

Query: 491 KTKSA 477
           K ++A
Sbjct: 175 KPRTA 179



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>NECP2_HUMAN (Q9NVZ3) Adaptin ear-binding coat-associated protein 2 (NECAP-2)|
          Length = 263

 Score = 37.4 bits (85), Expect = 0.040
 Identities = 16/51 (31%), Positives = 28/51 (54%)
 Frame = -2

Query: 659 EAYDFQAAXHDHMKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVLQIKN 507
           +A+DF  A  DH K++ ++    +  Q+ +    +D   KEG+T+ L I N
Sbjct: 114 DAFDFNVALQDHFKWVKQQCEFAKQAQNPDQGPKLDLGFKEGQTIKLNIAN 164



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>NECP1_HUMAN (Q8NC96) Adaptin ear-binding coat-associated protein 1 (NECAP-1)|
          Length = 275

 Score = 37.0 bits (84), Expect = 0.052
 Identities = 20/65 (30%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
 Frame = -2

Query: 659 EAYDFQAAXHDHMKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVL---QIKNKESG-S 492
           +A+DF  +  DH K++ ++    +  Q  +    +D   KEG+T+ L    I NK+ G S
Sbjct: 115 DAFDFNVSLQDHFKWVKQESEISKESQEMDARPKLDLGFKEGQTIKLCIGNITNKKGGAS 174

Query: 491 KTKSA 477
           K ++A
Sbjct: 175 KPRTA 179



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>NECP2_RAT (Q6P756) Adaptin ear-binding coat-associated protein 2 (NECAP-2)|
          Length = 263

 Score = 36.6 bits (83), Expect = 0.068
 Identities = 15/51 (29%), Positives = 28/51 (54%)
 Frame = -2

Query: 659 EAYDFQAAXHDHMKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVLQIKN 507
           +A+DF  A  DH K++ ++    +  Q+ +    +D   KEG+T+ + I N
Sbjct: 114 DAFDFNVALQDHFKWVKQQCEFAKQAQNPDEGPKLDLGFKEGQTIKINIAN 164



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>NECP2_MOUSE (Q9D1J1) Adaptin ear-binding coat-associated protein 2 (NECAP-2)|
          Length = 266

 Score = 36.2 bits (82), Expect = 0.089
 Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 3/64 (4%)
 Frame = -2

Query: 659 EAYDFQAAXHDHMKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVLQIKN---KESGSK 489
           +A+DF  A  DH K++ ++    +  Q+ +    +D   K+G+T+ + I N   KE  + 
Sbjct: 114 DAFDFNVALQDHFKWVKQQCEFAKQAQNPDEGPKLDLGFKDGQTIKINIANMRKKEGAAG 173

Query: 488 TKSA 477
           T  A
Sbjct: 174 TPRA 177



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>SPB1_KLULA (Q6CV12) AdoMet-dependent rRNA methyltransferase SPB1 (EC 2.1.1.-)|
           (2'-O-ribose RNA methyltransferase)
           (S-adenosyl-L-methionine-dependent methyltransferase)
          Length = 833

 Score = 31.2 bits (69), Expect = 2.9
 Identities = 20/62 (32%), Positives = 30/62 (48%)
 Frame = -2

Query: 605 KKTAEEMVQHYENTSSVDYSLKEGETLVLQIKNKESGSKTKSAFFEQGLNKLSFNDKANS 426
           K+T +E     +++  +D +  E  TL  Q+     G KTK    E+G N+ SF D  N 
Sbjct: 637 KRTKQE-----KHSKDIDIATVEAMTLAHQLA---LGHKTKHDLIEEGFNRYSFRDMENL 688

Query: 425 KE 420
            E
Sbjct: 689 PE 690



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>SN1L2_PONPY (Q5REX1) Serine/threonine-protein kinase SNF1-like kinase 2 (EC|
            2.7.11.1)
          Length = 925

 Score = 30.0 bits (66), Expect = 6.4
 Identities = 30/112 (26%), Positives = 47/112 (41%), Gaps = 14/112 (12%)
 Frame = -1

Query: 471  RARPEQAIVQ*QSKQQGGHSVPQNSHHLHPHPCLQLILELPCPLSKQNFLPRNKQAPAIL 292
            R + ++  +Q QS+ Q   +  Q +   +P P  QL      PL +Q   P ++QAP   
Sbjct: 716  RLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQL------PLPRQETPPPSQQAPPFS 769

Query: 291  KTSPL----------LSKQNFLHKKHWM----IPSKLKQRLPLVTNQLQQRR 178
             T PL          +    FL +   M    +PS    R   +  QLQQ++
Sbjct: 770  LTQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSSPRAAPLPTQLQQQQ 821



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>ATG2_DEBHA (Q6BTX0) Autophagy-related protein 2|
          Length = 1903

 Score = 30.0 bits (66), Expect = 6.4
 Identities = 19/72 (26%), Positives = 32/72 (44%)
 Frame = -2

Query: 623  MKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVLQIKNKESGSKTKSAFFEQGLNKLSF 444
            MK     +T E +    +N+ + D ++         ++    GS T S   E G NKLS 
Sbjct: 1130 MKSASHSETFENLTISRKNSDANDINI---------VEEYYDGSHTSSQSLENGFNKLSI 1180

Query: 443  NDKANSKEGTVS 408
            ++  N+K+   S
Sbjct: 1181 SESDNAKDDASS 1192



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>PRE1_STAAU (P03857) Plasmid recombination enzyme type 1 (Plasmid recombinase)|
           (Mobilization protein)
          Length = 403

 Score = 29.6 bits (65), Expect = 8.3
 Identities = 28/98 (28%), Positives = 40/98 (40%), Gaps = 3/98 (3%)
 Frame = -2

Query: 599 TAEEMVQHYENTSSVDYSLKEGETLVLQIKNKESGSKTKSAFFEQG--LNKLS-FNDKAN 429
           +AE +V  YEN  S DY  +  E    +   KE  +  K  + E+   +NKL   ND  N
Sbjct: 269 SAERIVDDYENIKSTDYYTENQELKKRRESLKEVVNTWKEGYHEKSKEVNKLKRENDSLN 328

Query: 428 SKEGTVSLKTPTTSTLTRVSN*FWSCHVPFQSRISFPG 315
            +         +T TL R             +R +FPG
Sbjct: 329 EQLNVSEKFQASTVTLYRA------------ARANFPG 354



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>GLB_TETTH (Q03459) Myoglobin (Hemoglobin)|
          Length = 121

 Score = 29.6 bits (65), Expect = 8.3
 Identities = 17/64 (26%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
 Frame = -2

Query: 644 QAAXHDHMKYLXKKKTAEEMVQHYENTSSVDYSLKEGETLVLQIKNKESGSKTKS-AFFE 468
           QAA H  +    KK  A++ V+HY   +++++  K+ E  +  +    +  K K+ A   
Sbjct: 14  QAAMHAAVPLFYKKVLADDRVKHYFKNTNMEHQAKQQEDFLTMLLGGPNHYKGKNMAEAH 73

Query: 467 QGLN 456
           +G+N
Sbjct: 74  KGMN 77


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,236,520
Number of Sequences: 219361
Number of extensions: 1609496
Number of successful extensions: 4699
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 4556
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4692
length of database: 80,573,946
effective HSP length: 108
effective length of database: 56,882,958
effective search space used: 6314008338
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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