Clone Information

BLAST Search Result

Clone Name	rbags31g17
Clone Library Name	barley_pub

No.	Definition	Score (bits)	E Value
1	PIM1_MOUSE (P06803) Proto-oncogene serine/threonine-protein kina...	32	1.1
2	ENPP3_HUMAN (O14638) Ectonucleotide pyrophosphatase/phosphodiest...	32	1.1
3	UBP42_HUMAN (Q9H9J4) Ubiquitin carboxyl-terminal hydrolase 42 (E...	32	1.4
4	ALL8_OLEEU (Q9M7R0) Calcium-binding allergen Ole e 8 (PCA18/PCA23)	32	1.8
5	CATR_GIALA (Q24956) Caltractin (Centrin)	31	2.4
6	SPE1A_STRPU (P04109) SPEC 1A protein	31	3.1
7	GUC1A_BOVIN (P46065) Guanylyl cyclase-activating protein 1 (GCAP...	30	4.0
8	ENPP3_RAT (P97675) Ectonucleotide pyrophosphatase/phosphodiester...	30	5.3
9	CALL_CAEEL (P04630) Calmodulin-like protein	30	5.3
10	TESC_MOUSE (Q9JKL5) Tescalcin (TSC)	30	5.3
11	CATR_CHLRE (P05434) Caltractin (Centrin) (20 kDa calcium-binding...	30	5.3
12	TNNC2_RABIT (P02586) Troponin C, skeletal muscle	30	6.9
13	TNNC2_PIG (P02587) Troponin C, skeletal muscle	30	6.9
14	TNNC2_MOUSE (P20801) Troponin C, skeletal muscle (STNC)	30	6.9
15	TNNC2_HUMAN (P02585) Troponin C, skeletal muscle	30	6.9
16	OMB_DROME (Q24432) Optomotor-blind protein (Lethal(1)optomotor-b...	30	6.9
17	FOXN2_HUMAN (P32314) Human T-cell leukemia virus enhancer factor...	30	6.9
18	TNNC2_ANGAN (P81660) Troponin C, skeletal muscle (TNC)	30	6.9
19	TNNC2_RANES (P02589) Troponin C, skeletal muscle	30	6.9
20	TNNC2_MELGA (P10246) Troponin C, skeletal muscle	30	6.9
21	TNNC2_CHICK (P02588) Troponin C, skeletal muscle	30	6.9
22	CAC1B_RABIT (Q05152) Voltage-dependent N-type calcium channel al...	29	9.0
23	CALL5_HUMAN (Q9NZT1) Calmodulin-like protein 5 (Calmodulin-like ...	29	9.0
24	TCH3_ARATH (P25071) Calmodulin-related protein 3, touch-induced	29	9.0

>PIM1_MOUSE (P06803) Proto-oncogene serine/threonine-protein kinase Pim-1 (EC|
           2.7.11.1)
          Length = 397

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 17/53 (32%), Positives = 26/53 (49%)
 Frame = -3

Query: 330 RGRPRRRQGSAQGAQCHLRLRHHPGRRAHPEIRRGRRVEEASVALRCAL*MHQ 172
           RG+PR+R  S+  +   LR      R A   +  GRR+  +S+  RC    H+
Sbjct: 23  RGQPRQRPQSSSDSPSALRASRSQSRNATRSLSPGRRLSPSSLRRRCCSSRHR 75

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>ENPP3_HUMAN (O14638) Ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP|
           3) (Phosphodiesterase I/nucleotide pyrophosphatase 3)
           (Phosphodiesterase I beta) (PD-Ibeta) (CD203c antigen)
           [Includes: Alkaline phosphodiesterase I (EC 3.1.4.1);
           Nucleotide p
          Length = 875

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 9/34 (26%)
 Frame = -2

Query: 157 CKCDVICRVKGDALWDL---------YFFCNAFR 83
           C+CDV C+ +GD  WD           + CN FR
Sbjct: 69  CRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFR 102

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>UBP42_HUMAN (Q9H9J4) Ubiquitin carboxyl-terminal hydrolase 42 (EC 3.1.2.15)|
            (Ubiquitin thioesterase 42)
            (Ubiquitin-specific-processing protease 42)
            (Deubiquitinating enzyme 42)
          Length = 1325

 Score = 32.0 bits (71), Expect = 1.4
 Identities = 21/57 (36%), Positives = 26/57 (45%)
 Frame = -3

Query: 414  RVTLCDQPQADHHAGRRPCAGAGDQKGNRGRPRRRQGSAQGAQCHLRLRHHPGRRAH 244
            R  L ++P  DH+ GRR C  A      R R R R  S +    H  L  HP R +H
Sbjct: 1085 RAGLHERPHKDHNRGRRGCEPA------RERERHRPSSPRAGAPH-ALAPHPDRFSH 1134

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>ALL8_OLEEU (Q9M7R0) Calcium-binding allergen Ole e 8 (PCA18/PCA23)|
          Length = 171

 Score = 31.6 bits (70), Expect = 1.8
 Identities = 13/33 (39%), Positives = 21/33 (63%)
 Frame = -1

Query: 515 SKERLKALMNEFDIDMNGLLDREEFAEFIRKLT 417
           SKE +  +M E D D +G ++ +EFA F++  T
Sbjct: 53  SKEEIGRIMEEIDTDKDGFINVQEFAAFVKAET 85

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>CATR_GIALA (Q24956) Caltractin (Centrin)|
          Length = 176

 Score = 31.2 bits (69), Expect = 2.4
 Identities = 15/36 (41%), Positives = 22/36 (61%)
 Frame = -1

Query: 515 SKERLKALMNEFDIDMNGLLDREEFAEFIRKLTAES 408
           S E L A++ EFD D +G +D E+F   +R  +A S
Sbjct: 141 SDEELLAMIQEFDRDGDGEIDEEDFIAILRSTSAFS 176

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>SPE1A_STRPU (P04109) SPEC 1A protein|
          Length = 152

 Score = 30.8 bits (68), Expect = 3.1
 Identities = 11/32 (34%), Positives = 23/32 (71%)
 Frame = -1

Query: 521 PPSKERLKALMNEFDIDMNGLLDREEFAEFIR 426
           P  ++++KA++ + D + +G +DREEF + I+
Sbjct: 119 PMKRKKIKAIIQKADANKDGKIDREEFMKLIK 150

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>GUC1A_BOVIN (P46065) Guanylyl cyclase-activating protein 1 (GCAP 1) (Guanylate|
           cyclase activator 1A)
          Length = 204

 Score = 30.4 bits (67), Expect = 4.0
 Identities = 13/40 (32%), Positives = 24/40 (60%)
 Frame = -1

Query: 512 KERLKALMNEFDIDMNGLLDREEFAEFIRKLTAESLCAIS 393
           +++L+     +D+D NG +DR+E    IR + A + C+ S
Sbjct: 88  EQKLRWYFKLYDVDGNGCIDRDELLTIIRAIRAINPCSDS 127

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>ENPP3_RAT (P97675) Ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP|
           3) (Phosphodiesterase I/nucleotide pyrophosphatase 3)
           (Phosphodiesterase I beta) (PD-Ibeta) (RB13-6 antigen)
           (B10) [Includes: Alkaline phosphodiesterase I (EC
           3.1.4.1); Nucleoti
          Length = 875

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 13/35 (37%), Positives = 17/35 (48%), Gaps = 9/35 (25%)
 Frame = -2

Query: 160 GCKCDVICRVKGDALWDL---------YFFCNAFR 83
           GC+CD  C  +GD  WD           + CN+FR
Sbjct: 69  GCRCDSGCTDRGDCCWDFEDTCVKSTQIWTCNSFR 103

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>CALL_CAEEL (P04630) Calmodulin-like protein|
          Length = 161

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 11/37 (29%), Positives = 24/37 (64%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEFAEFIRKLTAES 408
           P+++ +  ++NE DID NG ++  EF   ++++  E+
Sbjct: 57  PTEQEILEMINEVDIDGNGQIEFPEFCVMMKRMMKET 93

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>TESC_MOUSE (Q9JKL5) Tescalcin (TSC)|
          Length = 214

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 14/57 (24%), Positives = 29/57 (50%)
 Frame = -1

Query: 590 EVYIAVLCVYNDINKYLPGPHYDPPSKERLKALMNEFDIDMNGLLDREEFAEFIRKL 420
           E ++ ++  +  I+  L     +   KE+LK L + +D D +G +  EE+   + +L
Sbjct: 86  EDFLTIMSYFRPIDTTLGEEQVELSRKEKLKFLFHMYDSDSDGRITLEEYRNVVEEL 142

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>CATR_CHLRE (P05434) Caltractin (Centrin) (20 kDa calcium-binding protein)|
          Length = 169

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 14/36 (38%), Positives = 23/36 (63%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEFAEFIRKLTAE 411
           P KE +K +++E D D +G +D   F EF+  +TA+
Sbjct: 61  PKKEEIKKMISEIDKDGSGTID---FEEFLTMMTAK 93

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>TNNC2_RABIT (P02586) Troponin C, skeletal muscle|
          Length = 159

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEF 441
           P+KE L A++ E D D +G +D EEF
Sbjct: 50  PTKEELDAIIEEVDEDGSGTIDFEEF 75

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>TNNC2_PIG (P02587) Troponin C, skeletal muscle|
          Length = 159

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEF 441
           P+KE L A++ E D D +G +D EEF
Sbjct: 50  PTKEELDAIIEEVDEDGSGTIDFEEF 75

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>TNNC2_MOUSE (P20801) Troponin C, skeletal muscle (STNC)|
          Length = 159

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEF 441
           P+KE L A++ E D D +G +D EEF
Sbjct: 50  PTKEELDAIIEEVDEDGSGTIDFEEF 75

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>TNNC2_HUMAN (P02585) Troponin C, skeletal muscle|
          Length = 159

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEF 441
           P+KE L A++ E D D +G +D EEF
Sbjct: 50  PTKEELDAIIEEVDEDGSGTIDFEEF 75

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>OMB_DROME (Q24432) Optomotor-blind protein (Lethal(1)optomotor-blind) (L(1)omb)|
            (Protein bifid)
          Length = 972

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 11/29 (37%), Positives = 16/29 (55%)
 Frame = +1

Query: 64   QHQEAQTGRHYRKNTSPTGHHLSHGKSHH 150
            QHQ     +H+++   P  HH +H  SHH
Sbjct: 935  QHQSHHQQQHHQQPAQPHPHHQTHLHSHH 963

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>FOXN2_HUMAN (P32314) Human T-cell leukemia virus enhancer factor (Forkhead box|
           protein N2)
          Length = 341

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 18/50 (36%), Positives = 22/50 (44%)
 Frame = -3

Query: 456 GP*GVCGVHPEADRRVTLCDQPQADHHAGRRPCAGAGDQKGNRGRPRRRQ 307
           G  G  G H E D  V   D P  D     +PCA    +KG  G+  R+Q
Sbjct: 250 GSDGSEGFHSEEDTDVDYEDDPLGDSGYASQPCAKI-SEKGQSGKKMRKQ 298

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>TNNC2_ANGAN (P81660) Troponin C, skeletal muscle (TNC)|
          Length = 160

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -1

Query: 515 SKERLKALMNEFDIDMNGLLDREEFAEFIR 426
           S+E L      FD + +G +DREEF E +R
Sbjct: 92  SEEELAEFFRVFDKNGDGFIDREEFGEILR 121

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>TNNC2_RANES (P02589) Troponin C, skeletal muscle|
          Length = 162

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEF 441
           P+KE L A++ E D D +G +D EEF
Sbjct: 53  PTKEELDAIIEEVDEDGSGTIDFEEF 78

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>TNNC2_MELGA (P10246) Troponin C, skeletal muscle|
          Length = 162

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEF 441
           P+KE L A++ E D D +G +D EEF
Sbjct: 53  PTKEELDAIIEEVDEDGSGTIDFEEF 78

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>TNNC2_CHICK (P02588) Troponin C, skeletal muscle|
          Length = 162

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = -1

Query: 518 PSKERLKALMNEFDIDMNGLLDREEF 441
           P+KE L A++ E D D +G +D EEF
Sbjct: 53  PTKEELDAIIEEVDEDGSGTIDFEEF 78

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>CAC1B_RABIT (Q05152) Voltage-dependent N-type calcium channel alpha-1B subunit|
            (Voltage-gated calcium channel alpha subunit Cav2.2)
            (Calcium channel, L type, alpha-1 polypeptide isoform 5)
            (Brain calcium channel III) (BIII)
          Length = 2339

 Score = 29.3 bits (64), Expect = 9.0
 Identities = 21/82 (25%), Positives = 26/82 (31%), Gaps = 19/82 (23%)
 Frame = -3

Query: 429  PEADRRVTLCDQPQADHHAGRRPCAGAGDQKGNRGRPRR-------------------RQ 307
            P   R       P+     GR PC   G +   RG P                     ++
Sbjct: 893  PRRSRSKEAPGAPEVRSDRGRGPCPEGGRRHHRRGSPEEAAEREPRRHRAHRHGPDPGKE 952

Query: 306  GSAQGAQCHLRLRHHPGRRAHP 241
            G A G +   R RH  G RA P
Sbjct: 953  GPASGTRGERRARHRTGPRACP 974

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>CALL5_HUMAN (Q9NZT1) Calmodulin-like protein 5 (Calmodulin-like skin protein)|
          Length = 146

 Score = 29.3 bits (64), Expect = 9.0
 Identities = 12/31 (38%), Positives = 20/31 (64%)
 Frame = -1

Query: 521 PPSKERLKALMNEFDIDMNGLLDREEFAEFI 429
           P  +E L A++ E D+D +G ++ EEFA  +
Sbjct: 113 PLPQEELDAMIREADVDQDGRVNYEEFARML 143

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>TCH3_ARATH (P25071) Calmodulin-related protein 3, touch-induced|
          Length = 324

 Score = 29.3 bits (64), Expect = 9.0
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = -1

Query: 524 DPPSKERLKALMNEFDIDMNGLLDREEF 441
           + P+K  L+ LMNE D+D +G +D  EF
Sbjct: 42  EKPTKADLQDLMNEADLDGDGTIDFPEF 69


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,005,826
Number of Sequences: 219361
Number of extensions: 1165977
Number of successful extensions: 4626
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 4361
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4612
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5196311029
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

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full-length cDNA and EST

Clone Information

BLAST Search Result