ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbaal2a21
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SYG1_YEAST (P40528) Protein SYG1 32 1.1
2RF1_AERPE (Q9YAF1) Peptide chain release factor subunit 1 (Trans... 32 1.8
3EF2_METMA (Q8PUR7) Elongation factor 2 (EF-2) 31 3.1
4SYS_THET8 (Q5SJX7) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--... 30 5.3
5CARB_PSEPK (Q88DU6) Carbamoyl-phosphate synthase large chain (EC... 30 5.3
6UIDB_ECOLI (P30868) Glucuronide carrier protein (Glucuronide per... 30 5.3
7AT10A_HUMAN (O60312) Probable phospholipid-transporting ATPase V... 30 5.3
8PDR1_NICPL (Q949G3) Pleiotropic drug resistance protein 1 (NpPDR1) 30 6.9
9ABP1_SACEX (P38479) Actin-binding protein 29 9.0
10YBG0_YEAST (P34225) Hypothetical 78.8 kDa protein in SKT5-SHP1 i... 29 9.0
11TOP1P_MIMIV (Q5UQB5) DNA topoisomerase 1 type prokaryotic (EC 5.... 29 9.0

>SYG1_YEAST (P40528) Protein SYG1|
          Length = 902

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 21/67 (31%), Positives = 31/67 (46%)
 Frame = -3

Query: 417 SSSLDLAQTEKKPVKGLELFLESTKEVKALDSKPKTIVQNDAKKPTKTFDVPIKSAAVHR 238
           +S L  AQ +K+P+  LE  +       AL + PK    N  K    T    I    VHR
Sbjct: 337 TSELSSAQRDKEPITWLETQITEWFTT-ALTNSPKDRKHNTHKLKKLTIQYSISEQMVHR 395

Query: 237 SNRFLIR 217
           +NR +++
Sbjct: 396 NNRSIVQ 402



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>RF1_AERPE (Q9YAF1) Peptide chain release factor subunit 1 (Translation|
           termination factor aRF1)
          Length = 373

 Score = 31.6 bits (70), Expect = 1.8
 Identities = 19/52 (36%), Positives = 30/52 (57%), Gaps = 1/52 (1%)
 Frame = -3

Query: 519 GGAPGAHE-GRKSRRRFERL*HGSYPSFSKKRMKNSSSLDLAQTEKKPVKGL 367
           G  PG H+ G +S+RR+ER+       F KK  + +S+L +   EK  +KG+
Sbjct: 171 GFVPGKHKMGGQSQRRYERIIEQMVDEFFKKVGEEASNLLVPLAEKGVLKGV 222



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>EF2_METMA (Q8PUR7) Elongation factor 2 (EF-2)|
          Length = 730

 Score = 30.8 bits (68), Expect = 3.1
 Identities = 20/58 (34%), Positives = 30/58 (51%)
 Frame = -3

Query: 357 LESTKEVKALDSKPKTIVQNDAKKPTKTFDVPIKSAAVHRSNRFLIRKEGLVL*LMPA 184
           +E  K V+   SKP  + +   KK T+    PI+  + +R NRF I  E L L ++ A
Sbjct: 449 IERDKNVEITTSKPIVVYRETIKKKTE----PIEGKSPNRHNRFYISVEPLDLEIVSA 502



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>SYS_THET8 (Q5SJX7) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--tRNA ligase)|
           (SerRS)
          Length = 421

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 24/73 (32%), Positives = 30/73 (41%), Gaps = 11/73 (15%)
 Frame = +3

Query: 333 PLPPW---------CSPETIRDPSPASFQFEPGPRSLSFSSASWKNLDKSHVIGARTFSL 485
           PLPPW          + E  R  SP  F F P           W     S V G+R+++L
Sbjct: 102 PLPPWPGAPVGGEEANREIKRVGSPPEFSFPPLDHVALMEKNGWWEPRISQVSGSRSYAL 161

Query: 486 --IYALHELLELR 518
               AL+EL  LR
Sbjct: 162 KGDLALYELALLR 174



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>CARB_PSEPK (Q88DU6) Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)|
           (Carbamoyl-phosphate synthetase ammonia chain)
          Length = 1073

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 14/36 (38%), Positives = 23/36 (63%)
 Frame = +3

Query: 453 SHVIGARTFSLIYALHELLELRQYIRQAAIPKHDRP 560
           S+V+G R   ++Y   EL EL++Y+R+A    +D P
Sbjct: 716 SYVLGGRAMEIVY---ELDELKRYLREAVQVSNDSP 748



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>UIDB_ECOLI (P30868) Glucuronide carrier protein (Glucuronide permease)|
          Length = 457

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 26/81 (32%), Positives = 40/81 (49%)
 Frame = +2

Query: 119 LLLYFPIKQSKQECNVHHEATPAGINHSTRPSLRIKNLFDLCTAALFMGTSNVFVGFLAS 298
           ++LYF   +S +E  V   A P+ +N S +   R + LF LC  AL +  S   V   + 
Sbjct: 194 MVLYFICFKSTRENVVRIVAQPS-LNISLQTLKRNRPLFMLCIGALCVLISTFAVSASSL 252

Query: 299 FCTMVLGLESSAFTSLVLSRN 361
           F    +  ++  FT LVL +N
Sbjct: 253 FYVRYVLNDTGLFTVLVLVQN 273



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>AT10A_HUMAN (O60312) Probable phospholipid-transporting ATPase VA (EC 3.6.3.1)|
           (ATPVA) (Aminophospholipid translocase VA)
          Length = 1499

 Score = 30.0 bits (66), Expect = 5.3
 Identities = 18/42 (42%), Positives = 23/42 (54%), Gaps = 3/42 (7%)
 Frame = -3

Query: 591 GPHPRRRRPDG---VDRASEWPPVGCIGGAPGAHEGRKSRRR 475
           GP  RRRR +G     R++  PP G    A GA +G + RRR
Sbjct: 12  GPPGRRRRREGRTRTVRSNLLPPPGAEDPAAGAAKGERRRRR 53



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>PDR1_NICPL (Q949G3) Pleiotropic drug resistance protein 1 (NpPDR1)|
          Length = 1436

 Score = 29.6 bits (65), Expect = 6.9
 Identities = 30/111 (27%), Positives = 47/111 (42%), Gaps = 4/111 (3%)
 Frame = +2

Query: 206  RPSLRIKNLFDLCTAALFM---GTSNVFVGFLA-SFCTMVLGLESSAFTSLVLSRNNSRP 373
            +PS+ I   FD     LF+   G   ++VG L    C ++   ES    S ++   N  P
Sbjct: 1048 QPSIDIFEAFD----ELFLMKRGGQEIYVGPLGRQSCHLIKYFESIPGVSKIVEGYN--P 1101

Query: 374  FTGFFSV*ARSKELEFFIRFLEKLG*EPCHRRSNLLLDLRPS*APGAPPIH 526
             T    V A S+E+   + F +       +RR+  L+D      PG   +H
Sbjct: 1102 ATWMLEVTASSQEMALGVDFTDLYKKSDLYRRNKALIDELSVPRPGTSDLH 1152



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>ABP1_SACEX (P38479) Actin-binding protein|
          Length = 617

 Score = 29.3 bits (64), Expect = 9.0
 Identities = 15/46 (32%), Positives = 30/46 (65%), Gaps = 3/46 (6%)
 Frame = -3

Query: 441 FSKKRMKNSSSLDLAQTEKKPVK---GLELFLESTKEVKALDSKPK 313
           +++K+MK +   D AQ E+KPV+    +E+ ++++ E+K  D K +
Sbjct: 324 WAEKKMKQNQQSDSAQEEQKPVETKTEIEIGVDNSDEMKIGDLKSR 369



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>YBG0_YEAST (P34225) Hypothetical 78.8 kDa protein in SKT5-SHP1 intergenic|
           region
          Length = 687

 Score = 29.3 bits (64), Expect = 9.0
 Identities = 19/58 (32%), Positives = 33/58 (56%), Gaps = 3/58 (5%)
 Frame = -3

Query: 477 RFERL*HGSYPSFSKKRMKNSSSLDLAQ---TEKKPVKGLELFLESTKEVKALDSKPK 313
           +FE + +  Y  +S K +   +SL+L     +E KP+ GLEL  E  K+V+ ++ K +
Sbjct: 558 QFEAVSNAEY-GWSDKILTEHASLNLKNIVVSEWKPLLGLELLYEDAKDVEMVELKER 614



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>TOP1P_MIMIV (Q5UQB5) DNA topoisomerase 1 type prokaryotic (EC 5.99.1.2) (DNA|
           topoisomerase 1 type prokaryotic)
          Length = 854

 Score = 29.3 bits (64), Expect = 9.0
 Identities = 19/53 (35%), Positives = 26/53 (49%)
 Frame = -3

Query: 435 KKRMKNSSSLDLAQTEKKPVKGLELFLESTKEVKALDSKPKTIVQNDAKKPTK 277
           KK  K S+    ++T +KP +      +STK  K+  S  K  V   AKK TK
Sbjct: 797 KKTYKKSAPKGGSKTIRKPSQTKYSQTKSTKSTKSTKSTNKKFVGKSAKKTTK 849


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,818,056
Number of Sequences: 219361
Number of extensions: 2079221
Number of successful extensions: 6525
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 6183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6522
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5196311029
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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