ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbags22k06 |
|---|---|
| Clone Library Name | barley_pub |
>PRI2_ARATH (Q84WJ2) Probable DNA primase large subunit (EC 2.7.7.-)| Length = 454 Score = 239 bits (610), Expect = 5e-63 Identities = 105/151 (69%), Positives = 125/151 (82%) Frame = -1 Query: 626 GRMQXXXXXXXXXXXLEDAVTFWRSEFSQKVGSERFDKEYAYGIRHNYGKEGKRTDYTPY 447 GRMQ L+DA+ FWR EF++KVGSERFDKEYAY IRHNYGKEGKRTDYTPY Sbjct: 298 GRMQLGLFLKGVGLKLDDALAFWREEFTKKVGSERFDKEYAYAIRHNYGKEGKRTDYTPY 357 Query: 446 SCQKIISATPGVGDHHGCPYRHFGEENLRAALNNMGVSGNALEGILDKVKNRHYQLACTM 267 +C KII++ PG GDHHGCPYRHF E+NL+AAL+ MG+S +E ++DKV+NRHYQLACT+ Sbjct: 358 ACSKIITSAPGAGDHHGCPYRHFSEDNLKAALSRMGLSSRGMEDVMDKVRNRHYQLACTL 417 Query: 266 TFEATHGVSCDTGINHPNQYFSESQKVLQAK 174 TFEA +G SCDTGINHPNQYF ESQK+L++K Sbjct: 418 TFEAVYGTSCDTGINHPNQYFEESQKILKSK 448
>PRI2_MOUSE (P33610) DNA primase large subunit (EC 2.7.7.-) (DNA primase 58 kDa| subunit) (p58) Length = 505 Score = 152 bits (384), Expect = 8e-37 Identities = 74/151 (49%), Positives = 97/151 (64%), Gaps = 3/151 (1%) Frame = -1 Query: 626 GRMQXXXXXXXXXXXLEDAVTFWRSEFSQ-KVGSERFDKEYAYGIRHNYGKEGKRTDYTP 450 GRMQ LE A+ FW+ EF + K+ ++FDK Y+Y IRH++GKEGKRTDYTP Sbjct: 305 GRMQYGLFLKGIGLTLEQALQFWKQEFIRGKMDPDKFDKGYSYNIRHSFGKEGKRTDYTP 364 Query: 449 YSCQKIISATP-GVGDHHGCPYRHFGEENLRAALNNMGVSGNALEGILDKVKNRHYQLAC 273 +SC KII P G GD+HGCP+RH E L+ + + + + + ILD VK HYQ+AC Sbjct: 365 FSCMKIILTNPPGQGDYHGCPFRHSDAELLKQKMQSYKIPASGISQILDLVKGNHYQVAC 424 Query: 272 TMTFEATHGV-SCDTGINHPNQYFSESQKVL 183 FE TH V C +NHPNQ+F ESQ++L Sbjct: 425 QKYFEMTHNVDDCGFSLNHPNQFFFESQRIL 455
>PRI2_HUMAN (P49643) DNA primase large subunit (EC 2.7.7.-) (DNA primase 58 kDa| subunit) (p58) Length = 509 Score = 149 bits (377), Expect = 5e-36 Identities = 75/151 (49%), Positives = 96/151 (63%), Gaps = 3/151 (1%) Frame = -1 Query: 626 GRMQXXXXXXXXXXXLEDAVTFWRSEFSQ-KVGSERFDKEYAYGIRHNYGKEGKRTDYTP 450 GRMQ LE A+ FW+ EF + K+ ++FDK Y+Y IRH++GKEGKRTDYTP Sbjct: 305 GRMQYGLFLKGIGLTLEQALQFWKQEFIKGKMDPDKFDKGYSYNIRHSFGKEGKRTDYTP 364 Query: 449 YSCQKII-SATPGVGDHHGCPYRHFGEENLRAALNNMGVSGNALEGILDKVKNRHYQLAC 273 +SC KII S P GD+HGCP+RH E L+ L + +S + ILD VK HYQ+AC Sbjct: 365 FSCLKIILSNPPSQGDYHGCPFRHSDPELLKQKLQSYKISPGGISQILDLVKGTHYQVAC 424 Query: 272 TMTFEATHGV-SCDTGINHPNQYFSESQKVL 183 FE H V C +NHPNQ+F ESQ++L Sbjct: 425 QKYFEMIHNVDDCGFSLNHPNQFFCESQRIL 455
>PRI2_RAT (O89044) DNA primase large subunit (EC 2.7.7.-) (DNA primase 58 kDa| subunit) (p58) Length = 507 Score = 148 bits (374), Expect = 1e-35 Identities = 73/151 (48%), Positives = 95/151 (62%), Gaps = 3/151 (1%) Frame = -1 Query: 626 GRMQXXXXXXXXXXXLEDAVTFWRSEFSQ-KVGSERFDKEYAYGIRHNYGKEGKRTDYTP 450 GRMQ LE A+ FW+ EF + K+ ++FDK Y+Y IRH++GKEGKRTDYTP Sbjct: 305 GRMQYGLFLKGIGLTLEQALQFWKQEFIKGKMDPDKFDKGYSYNIRHSFGKEGKRTDYTP 364 Query: 449 YSCQKII-SATPGVGDHHGCPYRHFGEENLRAALNNMGVSGNALEGILDKVKNRHYQLAC 273 +SC KII + P GD HGCP+RH E L+ + + + + ILD VK HYQ+AC Sbjct: 365 FSCMKIILTNPPSQGDFHGCPFRHSDAELLKQKMQTYKIPASGISQILDLVKGNHYQVAC 424 Query: 272 TMTFEATHGV-SCDTGINHPNQYFSESQKVL 183 FE TH V C +NHPNQ+F ESQ++L Sbjct: 425 QKYFEMTHNVDDCGFSLNHPNQFFFESQRIL 455
>PRI2_DROME (Q9VPH2) DNA primase large subunit (EC 2.7.7.-)| Length = 533 Score = 142 bits (359), Expect = 6e-34 Identities = 63/145 (43%), Positives = 90/145 (62%) Frame = -1 Query: 626 GRMQXXXXXXXXXXXLEDAVTFWRSEFSQKVGSERFDKEYAYGIRHNYGKEGKRTDYTPY 447 GRMQ LED++ FWR EF++K+ +++F + Y Y I HNYGK+G +YTPY Sbjct: 316 GRMQYGLFLKGIGVALEDSLRFWREEFTKKMDADKFTRSYEYNIYHNYGKKGSMVNYTPY 375 Query: 446 SCQKIISATPGVGDHHGCPYRHFGEENLRAALNNMGVSGNALEGILDKVKNRHYQLACTM 267 SC KII G GD HGCPY++ + +L+ L++ G+S +A++ ++ V HYQ+ C Sbjct: 376 SCAKIIKDVAGPGDCHGCPYKNMDQGSLKTKLSSYGLSASAIDEVMFFVSRGHYQIGCGK 435 Query: 266 TFEATHGVSCDTGINHPNQYFSESQ 192 F+ TH + INHPN YF ESQ Sbjct: 436 YFQLTHNSPVEPSINHPNNYFEESQ 460
>PRI2_SCHPO (O74761) DNA primase large subunit (EC 2.7.7.-)| Length = 459 Score = 119 bits (298), Expect = 8e-27 Identities = 62/149 (41%), Positives = 89/149 (59%), Gaps = 5/149 (3%) Frame = -1 Query: 626 GRMQXXXXXXXXXXXLEDAVTFWRSEFSQKVGSERFDKEYAYGIRHNYGKEGKRTDYTPY 447 GR+Q +++A+ FWR F+ V ++F+KEY Y IRH YG EG R +Y Y Sbjct: 309 GRLQYGLFLKDIGLSVDEALVFWRKSFTN-VTEDKFNKEYRYNIRHTYGLEGNRKNYKGY 367 Query: 446 SCQKIISATP-GVGDHHGCPYRHFGEENLRAALNNMGVSGNA--LEGILDKVKNRHYQLA 276 +CQ+I++ G GD HGCPYR + NL +AL +MG++ ++ +++ VK RHY +A Sbjct: 368 NCQQILTGPQLGPGDAHGCPYRTYSVNNLVSALASMGIAPDSAGCREVVEAVKARHYHIA 427 Query: 275 CTMTFEATHG--VSCDTGINHPNQYFSES 195 CT FE TH S + I+HP QYF S Sbjct: 428 CTRVFELTHPNVGSLEESIHHPLQYFQLS 456
>PRI2_YEAST (P20457) DNA primase large subunit (EC 2.7.7.-) (DNA primase 58 kDa| subunit) (p58) Length = 528 Score = 118 bits (295), Expect = 2e-26 Identities = 62/168 (36%), Positives = 89/168 (52%), Gaps = 10/168 (5%) Frame = -1 Query: 626 GRMQXXXXXXXXXXXLEDAVTFWRSEFSQK--VGSERFDKEYAYGIRHNYGKEGKRTDYT 453 GR Q ++A+ FW F++ + E+F+KEY Y RHNYG EG R +Y Sbjct: 354 GRQQLSLFLKGIGLSADEALKFWSEAFTRNGNMTMEKFNKEYRYSFRHNYGLEGNRINYK 413 Query: 452 PYSCQKIISAT-PGVGDHHGCPYRHFGEENLRAALNNMGVSGNALEGILDKVKNRHYQLA 276 P+ C I+S PG GD+HGCP+R + E L A L +M ++ + +LD + Y +A Sbjct: 414 PWDCHTILSKPRPGRGDYHGCPFRDWSHERLSAELRSMKLTQAQIISVLDSCQKGEYTIA 473 Query: 275 CTMTFEATHG-------VSCDTGINHPNQYFSESQKVLQAKNQTVQSQ 153 CT FE TH + T I HPN YF S++ LQ K Q ++ + Sbjct: 474 CTKVFEMTHNSASADLEIGEQTHIAHPNLYFERSRQ-LQKKQQKLEKE 520
>PRI2_NEUCR (Q8NIZ4) Probable DNA primase large subunit (EC 2.7.7.-)| Length = 513 Score = 103 bits (258), Expect = 3e-22 Identities = 62/165 (37%), Positives = 85/165 (51%), Gaps = 21/165 (12%) Frame = -1 Query: 626 GRMQXXXXXXXXXXXLEDAVTFWRSEFSQKVGSERFDKEYAYGIRHNYGKEGKRTD---- 459 GR+Q LE+ + FWR F+ K+ + F+KEY Y +RH YG G ++ Sbjct: 333 GRLQYTLFLKGIGLNLEECLLFWRQSFN-KITDDTFNKEYRYNVRHTYGDVGGDSNRRGG 391 Query: 458 -YTPYSCQKIISA-TPGVGDHHGCPYRHFGEENLRAAL-NNMGVSG-NALEGILDKVKNR 291 Y+PYSCQKI++ PG G+ HGCPYRHF ENL+ L MGV+ L G+ + + + Sbjct: 392 GYSPYSCQKILTEHPPGPGEAHGCPYRHFNMENLQTLLQQGMGVTDRGVLNGVKEDKEKQ 451 Query: 290 HYQLACTMTFEATHGVSC----DTGIN---------HPNQYFSES 195 + +AC FE H D GI HPN+YF S Sbjct: 452 KFHMACNRVFEHLHKEELKKAKDEGIMTAAQLETIVHPNEYFKRS 496
>PRI2_CAEEL (O02334) DNA primase large subunit (EC 2.7.7.-)| Length = 503 Score = 101 bits (251), Expect = 2e-21 Identities = 53/137 (38%), Positives = 77/137 (56%), Gaps = 5/137 (3%) Frame = -1 Query: 578 EDAVTFWRSEFSQKVGSERFDKEYAYGIRHNYGKEGKRTDYTPYSCQKIISAT-PGVGDH 402 ++A+ R EF++K+ S++FDKEY Y IR+ YGKEG+R T SC II P D Sbjct: 343 DEAIAMMRDEFTKKITSDKFDKEYGYNIRYMYGKEGRRVAQTAMSCATIILRNPPSAVDC 402 Query: 401 HGCPYRHFGEENLRAALNNMGV-SGNALEGILDKVKNRHYQLACTMTFEATHGV---SCD 234 HGCP+RH ++ L+ L + + +E I + + Y ACT FE H + Sbjct: 403 HGCPFRHSEKQVLKQKLGSDAILKKEQIERIAELAELNQYDKACTRYFEFMHNLEEGGLG 462 Query: 233 TGINHPNQYFSESQKVL 183 I HPNQ++ ESQK++ Sbjct: 463 QLITHPNQFYEESQKIV 479 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 93,442,024 Number of Sequences: 219361 Number of extensions: 1969865 Number of successful extensions: 5109 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 4935 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5090 length of database: 80,573,946 effective HSP length: 107 effective length of database: 57,102,319 effective search space used: 5995743495 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
