ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbags18m23 |
|---|---|
| Clone Library Name | barley_pub |
>MTHR_ORYSA (Q75HE6) Probable methylenetetrahydrofolate reductase (EC 1.5.1.20)| Length = 594 Score = 212 bits (539), Expect = 4e-55 Identities = 100/114 (87%), Positives = 108/114 (94%) Frame = -1 Query: 452 QLIEKSKAFPSLTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWK 273 QLIEKSKAFPSLTYIAVNK+GES SNIP N VNAVTWGVFPGKEI+QPTVVDSASFMVWK Sbjct: 481 QLIEKSKAFPSLTYIAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMVWK 540 Query: 272 DEAFEIWSRGWACLFPEGDSXRELLEQVQKTYCLVSLVDNDYXSGDLFAAFKEI 111 DEAFEIWS+GWACLFPEGDS RE+L++VQK+Y LVSLVDNDY +GDLFAAFKEI Sbjct: 541 DEAFEIWSKGWACLFPEGDSSREILDKVQKSYFLVSLVDNDYINGDLFAAFKEI 594
>MTHR1_MAIZE (Q9SE94) Methylenetetrahydrofolate reductase 1 (EC 1.5.1.20)| (ZmMTHFR1) Length = 593 Score = 200 bits (509), Expect = 1e-51 Identities = 96/112 (85%), Positives = 101/112 (90%) Frame = -1 Query: 452 QLIEKSKAFPSLTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWK 273 QLIEK KAFPSLTYIAVNK+GE+ SNI N VNAVTWGVFPGKEIIQPTVVD ASFMVWK Sbjct: 481 QLIEKIKAFPSLTYIAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMVWK 540 Query: 272 DEAFEIWSRGWACLFPEGDSXRELLEQVQKTYCLVSLVDNDYXSGDLFAAFK 117 DEAFEIW+RGW C+FPEGDS RELLE+VQKTY LVSLVDNDY GDLFAAFK Sbjct: 541 DEAFEIWTRGWGCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQGDLFAAFK 592
>MTHR1_ARATH (Q9SE60) Methylenetetrahydrofolate reductase 1 (EC 1.5.1.20)| (AtMTHFR1) Length = 592 Score = 160 bits (405), Expect = 1e-39 Identities = 74/110 (67%), Positives = 90/110 (81%) Frame = -1 Query: 449 LIEKSKAFPSLTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWKD 270 L+EKSKAFPS+TY+AVNK +SN + VNAVTWGVFP KE+IQPT+VD ASF VWKD Sbjct: 482 LVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKVWKD 541 Query: 269 EAFEIWSRGWACLFPEGDSXRELLEQVQKTYCLVSLVDNDYXSGDLFAAF 120 EAFEIWSR WA L+PE D R+LLE+V+ +Y LVSLVDN+Y +GD+F+ F Sbjct: 542 EAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSVF 591
>MTHR2_ARATH (O80585) Methylenetetrahydrofolate reductase 2 (EC 1.5.1.20)| (AtMTHFR2) Length = 594 Score = 155 bits (391), Expect = 6e-38 Identities = 72/113 (63%), Positives = 88/113 (77%) Frame = -1 Query: 449 LIEKSKAFPSLTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWKD 270 ++EK KA PS+TY+AVNK + +SN VNAVTWGVFP KEIIQPT+VD ASF VWKD Sbjct: 482 VVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNVWKD 541 Query: 269 EAFEIWSRGWACLFPEGDSXRELLEQVQKTYCLVSLVDNDYXSGDLFAAFKEI 111 EAFE WSR WA L+PE D R LLE+V+ +Y LVSLV+NDY +GD+FA F ++ Sbjct: 542 EAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFADL 594
>MTHR_HUMAN (P42898) Methylenetetrahydrofolate reductase (EC 1.5.1.20)| Length = 656 Score = 107 bits (267), Expect = 1e-23 Identities = 48/91 (52%), Positives = 62/91 (68%) Frame = -1 Query: 419 LTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWKDEAFEIWSRGW 240 + Y VN +GE+I+N P NAVTWG+FPG+EIIQPTVVD SFM WKDEAF +W W Sbjct: 536 VNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIERW 595 Query: 239 ACLFPEGDSXRELLEQVQKTYCLVSLVDNDY 147 L+ E R +++ + Y LV+LVDND+ Sbjct: 596 GKLYEEESPSRTIIQYIHDNYFLVNLVDNDF 626
>MTHR_MACFA (Q60HE5) Methylenetetrahydrofolate reductase (EC 1.5.1.20)| Length = 656 Score = 107 bits (266), Expect = 2e-23 Identities = 48/91 (52%), Positives = 62/91 (68%) Frame = -1 Query: 419 LTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWKDEAFEIWSRGW 240 + Y VN +GE+I+N P NAVTWG+FPG+EIIQPTVVD SFM WKDEAF +W W Sbjct: 536 VNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQW 595 Query: 239 ACLFPEGDSXRELLEQVQKTYCLVSLVDNDY 147 L+ E R +++ + Y LV+LVDND+ Sbjct: 596 GKLYEEESPSRTIIQYIHDNYFLVNLVDNDF 626
>MTHR_MOUSE (Q9WU20) Methylenetetrahydrofolate reductase (EC 1.5.1.20)| Length = 654 Score = 103 bits (256), Expect = 3e-22 Identities = 46/91 (50%), Positives = 62/91 (68%) Frame = -1 Query: 419 LTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWKDEAFEIWSRGW 240 + Y V+ +GE+I+N P NAVTWG+FPG+EIIQPTVVD SFM WKDEAF +W W Sbjct: 535 VNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQW 594 Query: 239 ACLFPEGDSXRELLEQVQKTYCLVSLVDNDY 147 L+ E R +++ + Y LV+LVDN++ Sbjct: 595 GKLYEEESPSRMIIQYIHDNYFLVNLVDNEF 625
>MTHR1_SCHPO (Q10258) Methylenetetrahydrofolate reductase 1 (EC 1.5.1.20)| Length = 603 Score = 92.8 bits (229), Expect = 4e-19 Identities = 48/115 (41%), Positives = 68/115 (59%), Gaps = 1/115 (0%) Frame = -1 Query: 452 QLIEKSKAFPSLTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWK 273 +L E K S++Y NK G+ +N NAVTWGVFP +EIIQPT+V+S SF+ WK Sbjct: 474 ELKETVKKLNSVSYFVTNKNGDLDTNSQYEIPNAVTWGVFPNREIIQPTIVESTSFLAWK 533 Query: 272 DEAFEIWSRGWACLFPEGDSXRELLEQVQKTYCLVSLVDNDYXSG-DLFAAFKEI 111 DEA+ + WA + R+LL + K + L +VDND+ +G LF F ++ Sbjct: 534 DEAYSL-GMEWANAYSPDSISRKLLVSMMKEWFLCVIVDNDFQNGQSLFDVFNKM 587
>MTHR2_YEAST (P53128) Methylenetetrahydrofolate reductase 2 (EC 1.5.1.20)| Length = 599 Score = 91.7 bits (226), Expect = 8e-19 Identities = 46/109 (42%), Positives = 69/109 (63%), Gaps = 3/109 (2%) Frame = -1 Query: 452 QLIEKSKAFPSLTYIAVNKEGESISNIPXNXV-NAVTWGVFPGKEIIQPTVVDSASFMVW 276 +LI+ K LTY A++ +G+ +SN P N NAVTWG+FPG+EI+QPT+V+ SF+ W Sbjct: 482 KLIDTLKNNEFLTYFAIDSQGDLLSNHPDNSKSNAVTWGIFPGREILQPTIVEKISFLAW 541 Query: 275 KDEAFEIWSRGWACLFPEGDS--XRELLEQVQKTYCLVSLVDNDYXSGD 135 K+E + I + W + D + ++ + YCLV++VDNDY S D Sbjct: 542 KEEFYHILNE-WKLNMNKYDKPHSAQFIQSLIDDYCLVNIVDNDYISPD 589
>MTHR_CAEEL (Q17693) Probable methylenetetrahydrofolate reductase (EC 1.5.1.20)| Length = 663 Score = 84.7 bits (208), Expect = 1e-16 Identities = 44/104 (42%), Positives = 62/104 (59%), Gaps = 2/104 (1%) Frame = -1 Query: 452 QLIEKSKAFP-SLTYIAVNKEGE-SISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMV 279 Q+IE+ FP + Y A+NK+ +N AVTWGVFPG EI QPTVVD SF Sbjct: 543 QIIERE--FPVRVNYHAINKDSTFDKTNSEETTPIAVTWGVFPGSEIAQPTVVDPLSFRA 600 Query: 278 WKDEAFEIWSRGWACLFPEGDSXRELLEQVQKTYCLVSLVDNDY 147 W+DEA+++W W +P+ +++ V + LV+LVDND+ Sbjct: 601 WRDEAYQMWMAQWGDFYPKESKSYGVIKAVHDEFRLVTLVDNDF 644
>MTHR1_YEAST (P46151) Methylenetetrahydrofolate reductase 1 (EC 1.5.1.20)| Length = 657 Score = 84.7 bits (208), Expect = 1e-16 Identities = 40/94 (42%), Positives = 54/94 (57%) Frame = -1 Query: 416 TYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWKDEAFEIWSRGWA 237 +Y A + G +N+ + + VTWGVFP + Q T+++ SF W+DEAF IWS WA Sbjct: 555 SYYAGDSSGSFETNLDPHSSSVVTWGVFPNSPVKQTTIIEEESFKAWRDEAFSIWSE-WA 613 Query: 236 CLFPEGDSXRELLEQVQKTYCLVSLVDNDYXSGD 135 LFP LL V K YCLVS+V +D+ D Sbjct: 614 KLFPRNTPANILLRLVHKDYCLVSIVHHDFKETD 647
>MTHR2_SCHPO (O74927) Methylenetetrahydrofolate reductase 2 (EC 1.5.1.20)| Length = 641 Score = 84.0 bits (206), Expect = 2e-16 Identities = 37/93 (39%), Positives = 58/93 (62%) Frame = -1 Query: 425 PSLTYIAVNKEGESISNIPXNXVNAVTWGVFPGKEIIQPTVVDSASFMVWKDEAFEIWSR 246 P +TY A N + E ++N P + +AVTWGV+PG+EIIQ T++ SF W E+F++W Sbjct: 536 PQVTYYAGNNKSEFLTNAPKDGASAVTWGVYPGREIIQSTIIAEVSFKAWLSESFQVWGE 595 Query: 245 GWACLFPEGDSXRELLEQVQKTYCLVSLVDNDY 147 WA L+ + R+LLE LV+++ +D+ Sbjct: 596 -WANLYSKNTPSRKLLENCINDRWLVTVIHHDF 627
>PROS_RABIT (P98118) Vitamin K-dependent protein S precursor (Fragment)| Length = 646 Score = 32.7 bits (73), Expect = 0.44 Identities = 14/43 (32%), Positives = 22/43 (51%) Frame = -1 Query: 248 RGWACLFPEGDSXRELLEQVQKTYCLVSLVDNDYXSGDLFAAF 120 RGW + +E+++Q QK +CLV++ Y G A F Sbjct: 422 RGWNLMKQGASGVKEIIQQKQKKHCLVTVEKGSYYPGSGIAQF 464
>TNF10_HUMAN (P50591) Tumor necrosis factor ligand superfamily member 10| (TNF-related apoptosis-inducing ligand) (TRAIL protein) (Apo-2 ligand) (Apo-2L) (CD253 antigen) Length = 281 Score = 30.8 bits (68), Expect = 1.7 Identities = 25/86 (29%), Positives = 41/86 (47%), Gaps = 2/86 (2%) Frame = +2 Query: 20 VXWINRHKSRQIVLQDSNEGRSNT*DIIEIRSP*MQQRGP-RXCSHCPQG*PGNRSSAPA 196 V W R R+++L+ S E S + + SP +++RGP R +H G R + Sbjct: 80 VKWQLRQLVRKMILRTSEETISTVQEKQQNISPLVRERGPQRVAAHI----TGTRGRSNT 135 Query: 197 LATPWXSHPPGTGRPI-PWTRSQTLH 271 L++P + GR I W S++ H Sbjct: 136 LSSPNSKNEKALGRKINSWESSRSGH 161
>YB1C_SCHPO (P87177) Hypothetical WD-repeat protein C3D6.12 in chromosome II| Length = 922 Score = 30.8 bits (68), Expect = 1.7 Identities = 21/62 (33%), Positives = 31/62 (50%) Frame = -1 Query: 317 IQPTVVDSASFMVWKDEAFEIWSRGWACLFPEGDSXRELLEQVQKTYCLVSLVDNDYXSG 138 + PT V S S D + +W +G +F E + REL EQ + T LVS +N G Sbjct: 668 VGPTFVVSGSH----DHSIRLWEQGDDLVFLEEERERELEEQYEST--LVSSYENAEADG 721 Query: 137 DL 132 ++ Sbjct: 722 EV 723
>BUN2_DROME (Q24523) Protein bunched, class 2 isoform (Protein shortsighted)| Length = 1206 Score = 30.4 bits (67), Expect = 2.2 Identities = 20/54 (37%), Positives = 28/54 (51%), Gaps = 5/54 (9%) Frame = -1 Query: 377 NIPXNXVNAVTWGVFPG----KEIIQPTVVDSASFMVWKDEAFEIWSRG-WACL 231 N+ N +N V+ V PG K+ I+ T S F V K E+ E + RG W C+ Sbjct: 475 NVSDNIINVVSGAVTPGGTKKKDDIKETQHRSERFKVVKIESTEPFKRGRWMCM 528
>DXR_LEIXX (Q6AEY1) 1-deoxy-D-xylulose 5-phosphate reductoisomerase (EC| 1.1.1.267) (DXP reductoisomerase) (1-deoxyxylulose-5-phosphate reductoisomerase) (2-C-methyl-D-erythritol 4-phosphate synthase) Length = 361 Score = 30.0 bits (66), Expect = 2.8 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = +2 Query: 194 ALATPWXSHPPGTGRPIPWTRSQT 265 +L W PGTG P+ WTR+ T Sbjct: 247 SLGLGWPDRVPGTGLPLDWTRAHT 270
>KLK10_RAT (P36375) Glandular kallikrein-10 precursor (EC 3.4.21.35) (Tissue| kallikrein) (T-kininogenase) (K10) (Proteinase B) (Endopeptidase K) [Contains: T-kininogenase light chain; T-kininogenase heavy chain] (Fragment) Length = 244 Score = 29.3 bits (64), Expect = 4.8 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +3 Query: 246 PGPDLKRFIFPNHEARRVNDGRLDDLLAGEHAPSDSVHGVR 368 P PD K F+ NH +R +D D +L P+D GV+ Sbjct: 80 PHPDYKPFLMRNHTRQRGDDYSNDLMLLHLSEPADITDGVK 120
>XYLA_STRLI (Q9RFM4) Xylose isomerase (EC 5.3.1.5)| Length = 395 Score = 28.9 bits (63), Expect = 6.3 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = -1 Query: 263 FEIWSRGWACLFPEGDSXRELLEQVQKTYCLVSL 162 F +W+ GW L P GD+ RE L+ + L L Sbjct: 13 FGLWTVGWQGLDPFGDATREALDPAESVRRLSQL 46
>EMID1_HUMAN (Q96A84) EMI domain-containing protein 1 precursor (Protein Emu1)| (Emilin and multimerin domain-containing protein 1) Length = 441 Score = 28.9 bits (63), Expect = 6.3 Identities = 17/40 (42%), Positives = 19/40 (47%) Frame = +2 Query: 122 MQQRGPRXCSHCPQG*PGNRSSAPALATPWXSHPPGTGRP 241 M RGP PQG PG+ A A+ TP PPG P Sbjct: 217 MGMRGPPG----PQGPPGSPGRAGAVGTPGERGPPGPPGP 252
>PEP1A_STRCO (Q9L1K2) Putative glucanohydrolase pep1A (EC 3.2.1.-)| Length = 675 Score = 28.5 bits (62), Expect = 8.2 Identities = 13/53 (24%), Positives = 25/53 (47%) Frame = -1 Query: 347 TWGVFPGKEIIQPTVVDSASFMVWKDEAFEIWSRGWACLFPEGDSXRELLEQV 189 TWG++ G E+ + T + S E +++ R W EG + L+ ++ Sbjct: 507 TWGIYSGYELCENTPLREGSEEYLDSEKYQLKPRDWTRAAREGTTIAPLVTRL 559
>PEP1B_STRCO (Q9KY04) Putative glucanohydrolase pep1B (EC 3.2.1.-)| Length = 669 Score = 28.5 bits (62), Expect = 8.2 Identities = 13/53 (24%), Positives = 25/53 (47%) Frame = -1 Query: 347 TWGVFPGKEIIQPTVVDSASFMVWKDEAFEIWSRGWACLFPEGDSXRELLEQV 189 TWG++ G E+ + T + S E +++ R W EG + L+ ++ Sbjct: 498 TWGIYSGYELCENTPLREGSEEYLDSEKYQLKPRDWTRAAREGTTIAPLVTRL 550 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 62,761,896 Number of Sequences: 219361 Number of extensions: 1248233 Number of successful extensions: 3314 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 3196 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3308 length of database: 80,573,946 effective HSP length: 102 effective length of database: 58,199,124 effective search space used: 2793557952 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
