ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbags17g15 |
|---|---|
| Clone Library Name | barley_pub |
>YHI0_YEAST (P38708) Putative prolyl-tRNA synthetase YHR020W (EC 6.1.1.15)| (Proline--tRNA ligase) (ProRS) Length = 688 Score = 118 bits (296), Expect = 1e-26 Identities = 62/162 (38%), Positives = 92/162 (56%), Gaps = 13/162 (8%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLLE 455 +SQ+E+KG+P+RIE+GPKD+ QV +VRR++ K + +L ++ +L+ +Q +L + Sbjct: 527 FSQYELKGIPIRIELGPKDIEKNQVVVVRRNDSKKYVVSFDELEARIPEILEEMQGDLFK 586 Query: 454 TAKAKRDACIVIISTWDEFIAALNDKKLILAPWCDEEEVEKDVKART------------- 314 AK D VI++ W F+ ALN K +ILAPWC E E+D+K + Sbjct: 587 KAKELFDTHRVIVNEWSGFVPALNKKNVILAPWCGVMECEEDIKESSAKKDDGEEFEEDD 646 Query: 313 KGELGAAKTLCTPFDQPDLPSGTVCFASGKPAQKWSFWGRSY 188 K AK+LC PFDQP L G C + A + +GRSY Sbjct: 647 KAPSMGAKSLCIPFDQPVLNEGQKCIKCERIAVNYCMFGRSY 688
>SYEP_DROME (P28668) Bifunctional aminoacyl-tRNA synthetase [Includes:| Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase); Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA ligase)] Length = 1714 Score = 102 bits (254), Expect = 1e-21 Identities = 59/161 (36%), Positives = 93/161 (57%), Gaps = 12/161 (7%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLLE 455 ++ WE+KGVPLR+E+GPKDL +Q+ VRRD K+ IP D+ +++ LL+ I ++L Sbjct: 1555 FNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITIPLADVEKKIPALLETIHESMLN 1614 Query: 454 TAKAKRDACIVIISTWDEFIAALNDKKLILAPWCDEEEVEKDVKART----KGELGA--- 296 A+ + ++ W +F L K ++LAP+C E E +KA + + E GA Sbjct: 1615 KAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGEISCEDKIKADSARGEEAEPGAPAM 1674 Query: 295 -AKTLCTPFDQP-DLPSGTVCF---ASGKPAQKWSFWGRSY 188 AK+LC PFDQP + + C + KP + ++ +GRSY Sbjct: 1675 GAKSLCIPFDQPAPIAASDKCINPSCTNKP-KFYTLFGRSY 1714
>SYEP_HUMAN (P07814) Bifunctional aminoacyl-tRNA synthetase [Includes:| Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase); Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA ligase)] Length = 1440 Score = 94.0 bits (232), Expect = 3e-19 Identities = 52/158 (32%), Positives = 86/158 (54%), Gaps = 9/158 (5%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLLE 455 ++ WE+KGVP+R+E+GP+D+ + Q VRRD G K+ + + +++ +L+ IQ L Sbjct: 1283 FNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFT 1342 Query: 454 TAKAKRDACIVIISTWDEFIAALNDKKLILAPWCDEEEVEKDVKARTKG----ELGA--- 296 A +V+ +T ++F L+ K++ P+C E + E +K T E GA Sbjct: 1343 RASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSM 1402 Query: 295 -AKTLCTPFDQ-PDLPSGTVCFASGKPAQKWSFWGRSY 188 AK+LC PF +L G C PA+ ++ +GRSY Sbjct: 1403 GAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY 1440
>SYEP_MOUSE (Q8CGC7) Bifunctional aminoacyl-tRNA synthetase [Includes:| Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase); Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA ligase)] Length = 1512 Score = 92.4 bits (228), Expect = 1e-18 Identities = 54/158 (34%), Positives = 87/158 (55%), Gaps = 9/158 (5%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLLE 455 ++ WE+KGVP+R+E+GP+D+ + Q VRRD G K+ I + ++ +L+ IQ NL Sbjct: 1355 FNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAKLEKVLEDIQLNLFT 1414 Query: 454 TAKAKRDACIVIISTWDEFIAALNDKKLILAPWCDEEEVEKDVKARTKG----ELGA--- 296 A +V+ +T ++F L+ K+ P+C E + E +K T E GA Sbjct: 1415 RASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDVEPGAPSM 1474 Query: 295 -AKTLCTPFDQ-PDLPSGTVCFASGKPAQKWSFWGRSY 188 AK+LC PF+ +L G +C PA+ ++ +GRSY Sbjct: 1475 GAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY 1512
>SYP_CLOST (Q9L4Q8) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA| ligase) (ProRS) Length = 481 Score = 90.1 bits (222), Expect = 5e-18 Identities = 55/155 (35%), Positives = 81/155 (52%), Gaps = 6/155 (3%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLLE 455 ++Q+EMKGVPLR+EIGP+D+ N I RRD +K ++ + V+ LLD + ++LE Sbjct: 339 FNQYEMKGVPLRLEIGPRDIENNVAMIARRDTLSKDSYSLDNIGDTVKNLLDTVHTDMLE 398 Query: 454 TAKAKRDACIVIISTWDEFIAALNDKKLILAP------WCDEEEVEKDVKARTKGELGAA 293 A+A RD+ ++EF +K+I P WC EEE E +K T Sbjct: 399 RARAHRDSKTFTFKDYEEF-----KRKMIETPGFAKGMWCGEEECEAKIKEDT-----GV 448 Query: 292 KTLCTPFDQPDLPSGTVCFASGKPAQKWSFWGRSY 188 C PF Q +L G C GKPA+ + ++Y Sbjct: 449 TIRCIPFVQENL--GETCQFCGKPAKHMVYLAKAY 481
>SYPC_METJA (Q58635) Bifunctional aminoacyl-tRNA synthetase (ProCysRS)| (AATS-CysPro) [Includes: Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA ligase); Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cysteine--tRNA ligase)] Length = 455 Score = 77.0 bits (188), Expect = 4e-14 Identities = 46/116 (39%), Positives = 69/116 (59%), Gaps = 6/116 (5%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLLE 455 ++ WE+KGVPLRIE+GPKD+ NK++ + RRD K + T L+E V L+ I N+ Sbjct: 325 FNDWEIKGVPLRIEVGPKDIENKKITLFRRDTMEKFQVDETQLMEVVEKTLNNIMENIKN 384 Query: 454 TAKAKRDACIVIIS--TWDEFIAALNDKK-LILAPWCDE---EEVEKDVKARTKGE 305 A K + I I+ DE L++K+ +IL P+ +E EE+E+ V+A GE Sbjct: 385 RAWEKFENFITILEDINPDEIKNILSEKRGVILVPFKEEIYNEELEEKVEATILGE 440
>SYP_METTH (O26708) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA| ligase) (ProRS) Length = 482 Score = 72.4 bits (176), Expect = 1e-12 Identities = 41/112 (36%), Positives = 63/112 (56%), Gaps = 1/112 (0%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLLE 455 Y +WEM+GVPLR+EIGP+DL I RRD G KV + E +R L+ I NL Sbjct: 342 YYEWEMRGVPLRVEIGPRDLEKGAAVISRRDTGEKVTADLQGIEETLRELMKDILENLRT 401 Query: 454 TAKAKRDACIVIISTWDEFIAALNDKKLILA-PWCDEEEVEKDVKARTKGEL 302 A + ++ I T +E +++K+ I++ WC EEE DV+ + + ++ Sbjct: 402 RAWERMESEIREAETLEEASRIVDEKRGIISFMWCGEEECGMDVEEKVRVDI 453
>SYP_MYCLE (Q9Z5I7) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA| ligase) (ProRS) Length = 480 Score = 65.9 bits (159), Expect = 1e-10 Identities = 43/121 (35%), Positives = 60/121 (49%), Gaps = 1/121 (0%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDL-ANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLL 458 Y++WE++GVP+R+E+GP+DL A V + R AK I ++ +L+ Q LL Sbjct: 337 YNEWELRGVPIRLELGPRDLEAGTTVMVRRIGEKAKQPIAIAAAPTELPGILEEFQRTLL 396 Query: 457 ETAKAKRDACIVIISTWDEFIAALNDKKLILAPWCDEEEVEKDVKARTKGELGAAKTLCT 278 E A RD ++ WD F A LA C E E+D+KA T AA C Sbjct: 397 ERATQFRDDHTTLVDNWDAF-ATTVATGWALAIHCGNPECEEDIKAET-----AATPRCV 450 Query: 277 P 275 P Sbjct: 451 P 451
>SYP_BORBU (O51363) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA| ligase) (ProRS) Length = 488 Score = 55.1 bits (131), Expect = 2e-07 Identities = 48/159 (30%), Positives = 72/159 (45%), Gaps = 10/159 (6%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGA--KVDIPSTDLVEQVRVLLDGIQANL 461 +S E KG+P+R+E+G D+ V I RRD K I L+ +V+V LD +Q +L Sbjct: 336 FSSAEFKGIPIRLEVGINDVLLNSVTISRRDKDRKFKYQISLDSLISKVKVELDLMQKDL 395 Query: 460 LETAKAKRDACIVII-----STWDEFIAALND-KKLILAPWCDEEEVEKDVKARTKGELG 299 + A R I +++ F A +ND +L+ WC E +K TK Sbjct: 396 FQRALNFRILNTKEIFRSSKDSYETFKAYVNDYSGFVLSCWCGSLNCENIIKNETK---- 451 Query: 298 AAKTLCTP--FDQPDLPSGTVCFASGKPAQKWSFWGRSY 188 A C P F DL +G C A+ + + +SY Sbjct: 452 -ATIRCIPDDFKARDL-TGMTCIYCSSKAKYFVLFAKSY 488
>SYP_ARCFU (O28664) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA| ligase) (ProRS) Length = 575 Score = 44.7 bits (104), Expect = 2e-04 Identities = 25/51 (49%), Positives = 35/51 (68%), Gaps = 1/51 (1%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVR-RDNGAKVDIPSTDLVEQVRVL 485 Y +WE+KGVP+R EIGP+D A K V +V RD K ++P D V++ +VL Sbjct: 433 YYKWELKGVPIRFEIGPRD-AEKGVAVVSFRDEKRKFEVP-LDEVDEGKVL 481
>VGLM_EBV (P03215) Glycoprotein M| Length = 405 Score = 32.7 bits (73), Expect = 0.93 Identities = 22/47 (46%), Positives = 26/47 (55%), Gaps = 2/47 (4%) Frame = -2 Query: 178 CSATYTLPFSLSV--CSGKSVYFHFIDRLFWQICLFLILPDF*VMLS 44 C A TL FSLSV G S YF D + + LFLILP F +L+ Sbjct: 212 CLALETLVFSLSVFLALGNSFYFMVGDMVLGAVNLFLILPIFWYILT 258
>SYP_MYCPN (P75382) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA| ligase) (ProRS) Length = 483 Score = 32.0 bits (71), Expect = 1.6 Identities = 34/147 (23%), Positives = 61/147 (41%) Frame = -2 Query: 628 QWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQANLLETA 449 Q E+ G+P ++ + N R+ R + ++ VE L+ + + A Sbjct: 349 QGEVNGIPFQLIVDNPQTVNIFNRLTRVKTAYSFEQLASRFVE----LVQQYHQAMYDKA 404 Query: 448 KAKRDACIVIISTWDEFIAALNDKKLILAPWCDEEEVEKDVKARTKGELGAAKTLCTPFD 269 KA +V +T + A NDKK +L + +E+ +K R + T+ + Sbjct: 405 KAVVQQKVVQATTLKQIEQAFNDKKAVLCAVRLTDTLEQQLKERYQ------VTVRCCLE 458 Query: 268 QPDLPSGTVCFASGKPAQKWSFWGRSY 188 Q P +C SG+ AQ + R+Y Sbjct: 459 QLQKPQ--ICPFSGESAQDYVLIARAY 483
>UNC52_CAEEL (Q06561) Basement membrane proteoglycan precursor (Perlecan homolog)| (Uncoordinated protein 52) Length = 3375 Score = 31.6 bits (70), Expect = 2.1 Identities = 30/124 (24%), Positives = 51/124 (41%), Gaps = 16/124 (12%) Frame = -2 Query: 592 IGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLDGIQAN-----LLETAKAKRDAC 428 IG + Q + ++ G D+PS VEQ ++L + + E K + Sbjct: 2468 IGKTETKPNQSVVWTKEEG---DLPSGSRVEQGVLMLPSVHRDDEGSYTCEIVKEENPVF 2524 Query: 427 IVIISTWDEFIAALNDKKLILAPWCDEEEVEKDVK-----ARTKG------ELGAAKTLC 281 + D+FI ++ + + L P DEE V D++ A KG + + L Sbjct: 2525 STVDLQIDDFIPVIDGEPIELPPLSDEEIVNLDIEITLNTANPKGIIFETKRINSGDLLA 2584 Query: 280 TPFD 269 TP+D Sbjct: 2585 TPYD 2588
>SYH_PYRAB (Q9UY31) Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA| ligase) (HisRS) Length = 431 Score = 31.6 bits (70), Expect = 2.1 Identities = 17/45 (37%), Positives = 28/45 (62%) Frame = -2 Query: 613 GVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLLD 479 GV L I +G +DL V I ++G +V++P ++V +VR LL+ Sbjct: 386 GVRLSIIVGKRDLERGVVTIRDLESGNQVEVPVDNVVTKVRELLN 430
>TPR_HUMAN (P12270) Nucleoprotein TPR| Length = 2349 Score = 31.2 bits (69), Expect = 2.7 Identities = 16/33 (48%), Positives = 23/33 (69%) Frame = -2 Query: 580 DLANKQVRIVRRDNGAKVDIPSTDLVEQVRVLL 482 D ANKQ ++ RDN +++I DL +Q+RVLL Sbjct: 473 DKANKQSSVLERDN-RRMEIQVKDLSQQIRVLL 504
>SYP_ECOLI (P16659) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA| ligase) (ProRS) (Global RNA synthesis factor) Length = 572 Score = 30.8 bits (68), Expect = 3.5 Identities = 15/45 (33%), Positives = 25/45 (55%) Frame = -2 Query: 634 YSQWEMKGVPLRIEIGPKDLANKQVRIVRRDNGAKVDIPSTDLVE 500 ++ E+ G+P I +G ++L N + R NG K I + D+VE Sbjct: 520 FADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVE 564
>DPOM_AGABT (P30322) Probable DNA polymerase (EC 2.7.7.7) (Fragment)| Length = 797 Score = 30.8 bits (68), Expect = 3.5 Identities = 16/46 (34%), Positives = 21/46 (45%) Frame = -2 Query: 442 KRDACIVIISTWDEFIAALNDKKLILAPWCDEEEVEKDVKARTKGE 305 KR C+ TWD+ + N K WCDE E + R+ GE Sbjct: 72 KRVVCLADNKTWDQSLKYWNSSKYPYPRWCDEYLTESEF-VRSIGE 116
>GNT1_GIBZE (Q4HVS2) Glucose N-acetyltransferase 1 (EC 2.4.1.-)| (N-acetylglucosaminyltransferase) Length = 431 Score = 30.0 bits (66), Expect = 6.0 Identities = 21/75 (28%), Positives = 28/75 (37%), Gaps = 3/75 (4%) Frame = -2 Query: 409 WDEFIAALNDKKLILAPWCDEEEVEKDVKARTKGELGAAKTLCTPFDQPDLPSGT---VC 239 WD +A K + + W + DV+ R +QPD P VC Sbjct: 369 WDSSVALSEAKFVHFSDWPVPKPWINDVETRLA-------------NQPDCPEDNKKRVC 415 Query: 238 FASGKPAQKWSFWGR 194 + GK QKW W R Sbjct: 416 ESVGKTGQKWIHWRR 430
>LOLA_SHEON (Q8EER2) Outer-membrane lipoprotein carrier protein precursor| Length = 206 Score = 30.0 bits (66), Expect = 6.0 Identities = 17/39 (43%), Positives = 20/39 (51%) Frame = +2 Query: 359 WSQDQLFVIESCYEFIPSANDYNAGISLCFRCFQQVCLN 475 WSQ + + CYE P A D AGIS CF + LN Sbjct: 122 WSQYAVTKKQDCYEIKPKATD--AGISAVNVCFNKGTLN 158 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 96,162,087 Number of Sequences: 219361 Number of extensions: 2063328 Number of successful extensions: 6313 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 6068 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 6300 length of database: 80,573,946 effective HSP length: 107 effective length of database: 57,102,319 effective search space used: 5938641176 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
