ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbags14n17
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1GSH1_ARATH (P46309) Glutamate--cysteine ligase, chloroplast prec... 88 1e-25
2GSH1_BRAJU (O23736) Glutamate--cysteine ligase, chloroplast prec... 87 2e-24
3GSH1_LYCES (O22493) Glutamate--cysteine ligase, chloroplast prec... 77 6e-23
4GSH1_MEDTR (Q9ZNX6) Glutamate--cysteine ligase, chloroplast prec... 79 4e-22
5BRE1_DEBHA (Q6BWW6) Ubiquitin-protein ligase BRE1 (EC 6.3.2.-) 34 0.39
6TYCC_BREPA (O30409) Tyrocidine synthetase 3 (Tyrocidine syntheta... 31 2.5
7CCNB3_CANFA (Q659K0) G2/mitotic-specific cyclin-B3 31 3.3
8RSSA_CAEEL (P46769) Probable 40S ribosomal protein SA (p40) 30 4.3
9MASZ_BACHD (Q9KB03) Malate synthase G (EC 2.3.3.9) 30 4.3
10TKT1_KLULA (Q12630) Transketolase (EC 2.2.1.1) (TK) 30 5.6
11NUDH_ZYMMO (Q9RH11) Probable (di)nucleoside polyphosphate hydrol... 30 5.6
12IKAR_MOUSE (Q03267) DNA-binding protein Ikaros (Lymphoid transcr... 30 5.6
13ORCT3_MOUSE (Q6A4L0) Organic cation transporter-like 3 (Solute c... 30 7.3
14PCNT_MOUSE (P48725) Pericentrin 29 9.6
15SYFB_PSEPK (Q88K22) Phenylalanyl-tRNA synthetase beta chain (EC ... 29 9.6

>GSH1_ARATH (P46309) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 522

 Score = 88.2 bits (217), Expect(2) = 1e-25
 Identities = 42/52 (80%), Positives = 47/52 (90%)
 Frame = -1

Query: 615 KVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTG 460
           KVPVTGLKTPFRDG ++ +AEDVL+LAK+GLERRGYKE GFL  VDEVVRTG
Sbjct: 443 KVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDEVVRTG 494



 Score = 47.8 bits (112), Expect(2) = 1e-25
 Identities = 21/28 (75%), Positives = 24/28 (85%)
 Frame = -2

Query: 458 VTPAEKLLNLYETKWQRSVDPVFEELLY 375
           VTPAEKLL +Y  +W +SVDPVFEELLY
Sbjct: 495 VTPAEKLLEMYNGEWGQSVDPVFEELLY 522



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>GSH1_BRAJU (O23736) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 514

 Score = 87.0 bits (214), Expect(2) = 2e-24
 Identities = 42/52 (80%), Positives = 47/52 (90%)
 Frame = -1

Query: 615 KVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTG 460
           KVPVTGLKTPFRDG ++ +AEDVL+LAK+GLERRGYKEVGFL  V EVVRTG
Sbjct: 435 KVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEVGFLNAVTEVVRTG 486



 Score = 44.7 bits (104), Expect(2) = 2e-24
 Identities = 19/28 (67%), Positives = 23/28 (82%)
 Frame = -2

Query: 458 VTPAEKLLNLYETKWQRSVDPVFEELLY 375
           VTPAE LL +Y  +W +SVDPVF+ELLY
Sbjct: 487 VTPAENLLEMYNGEWGQSVDPVFQELLY 514



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>GSH1_LYCES (O22493) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 523

 Score = 77.0 bits (188), Expect(2) = 6e-23
 Identities = 36/52 (69%), Positives = 44/52 (84%)
 Frame = -1

Query: 615 KVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTG 460
           KVP +GLKTPFRDG +  +A+DV++LAK GLERRG+KE GFL EV EVV+TG
Sbjct: 444 KVPKSGLKTPFRDGLLMHVAQDVVKLAKEGLERRGFKETGFLNEVAEVVKTG 495



 Score = 50.1 bits (118), Expect(2) = 6e-23
 Identities = 22/34 (64%), Positives = 28/34 (82%)
 Frame = -2

Query: 476 KLLEQEVTPAEKLLNLYETKWQRSVDPVFEELLY 375
           ++++  VTPAEKLL LY  KW +SVDP+FEELLY
Sbjct: 490 EVVKTGVTPAEKLLELYHGKWGQSVDPIFEELLY 523



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>GSH1_MEDTR (Q9ZNX6) Glutamate--cysteine ligase, chloroplast precursor (EC|
           6.3.2.2) (Gamma-glutamylcysteine synthetase) (Gamma-ECS)
           (GCS)
          Length = 508

 Score = 78.6 bits (192), Expect(2) = 4e-22
 Identities = 38/52 (73%), Positives = 45/52 (86%)
 Frame = -1

Query: 615 KVPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTG 460
           KV VTGLKTPFRDG ++ +AE+VL+LAK+GLERRG+KE GFL  V EVVRTG
Sbjct: 429 KVTVTGLKTPFRDGLLKHVAEEVLELAKDGLERRGFKESGFLNAVAEVVRTG 480



 Score = 45.8 bits (107), Expect(2) = 4e-22
 Identities = 20/28 (71%), Positives = 24/28 (85%)
 Frame = -2

Query: 458 VTPAEKLLNLYETKWQRSVDPVFEELLY 375
           VTPAE+LL LY  KW++SVD VF+ELLY
Sbjct: 481 VTPAERLLELYHGKWEQSVDHVFDELLY 508



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>BRE1_DEBHA (Q6BWW6) Ubiquitin-protein ligase BRE1 (EC 6.3.2.-)|
          Length = 691

 Score = 33.9 bits (76), Expect = 0.39
 Identities = 24/61 (39%), Positives = 35/61 (57%)
 Frame = -1

Query: 597 LKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDEVVRTGSDTC*EASESIRDE 418
           LK+ F +  V DL   +L L K+  +RR  K    L+ +DE +  GSD   EASE+++ E
Sbjct: 175 LKSQF-ESKVEDLQAQLLTLVKDN-DRRSSKT---LQRIDESLTNGSDVKEEASETVKSE 229

Query: 417 V 415
           V
Sbjct: 230 V 230



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>TYCC_BREPA (O30409) Tyrocidine synthetase 3 (Tyrocidine synthetase III)|
            [Includes: ATP-dependent asparagine adenylase (AsnA)
            (Asparagine activase); ATP-dependent glutamine adenylase
            (GlnA) (Glutamine activase); ATP-dependent tyrosine
            adenylase (TyrA) (Ty
          Length = 6486

 Score = 31.2 bits (69), Expect = 2.5
 Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 4/64 (6%)
 Frame = +3

Query: 177  TGERKTASH---KQSVPSSKLFAAARPEVGFPRQVACGIEDGIF-VQGYSLKTLSTLSSV 344
            +GER T S     Q+   +KL    +  +G P   A GI D  F + G+SLK ++ ++ V
Sbjct: 4064 SGERTTGSAFVAAQNDTEAKLQQIWQEVLGIP---AIGIHDNFFEIGGHSLKAMNVITQV 4120

Query: 345  HRSY 356
            H+++
Sbjct: 4121 HKTF 4124



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>CCNB3_CANFA (Q659K0) G2/mitotic-specific cyclin-B3|
          Length = 1330

 Score = 30.8 bits (68), Expect = 3.3
 Identities = 13/29 (44%), Positives = 14/29 (48%)
 Frame = +1

Query: 289  TGFLYRDTV*RPCPPCPASTEVICDSVYQ 375
            T FL       PCPPC      ICD +YQ
Sbjct: 1152 TAFLIAAKFEEPCPPCVDDFLYICDDIYQ 1180



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>RSSA_CAEEL (P46769) Probable 40S ribosomal protein SA (p40)|
          Length = 275

 Score = 30.4 bits (67), Expect = 4.3
 Identities = 19/55 (34%), Positives = 26/55 (47%)
 Frame = +1

Query: 82  QGKVKKGSVQTGVCSGSLEMREKS*VVKDPPQLEKEKQQATNRVFRAQNYSQPRD 246
           +GK+ +   QTG      E+       +DP + EKE+  A   V  AQ Y QP D
Sbjct: 185 RGKISR---QTGFVLEGKEIMPDLYFYRDPTETEKEETGAHADVAEAQEYQQPTD 236



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>MASZ_BACHD (Q9KB03) Malate synthase G (EC 2.3.3.9)|
          Length = 727

 Score = 30.4 bits (67), Expect = 4.3
 Identities = 19/67 (28%), Positives = 32/67 (47%)
 Frame = -1

Query: 507 KEVGFLREVDEVVRTGSDTC*EASESIRDEVAAQRGPRLRGVVILIH*ITYNFGGRWTRW 328
           +E+G+L  + E V          +E++ DE+AAQ GP+L   V+ ++   Y       RW
Sbjct: 85  EEIGYLEPIPEHVTI-------TTENVDDEIAAQAGPQL---VVPVNNARYAINAANARW 134

Query: 327 TGSSDCI 307
               D +
Sbjct: 135 GSLYDAL 141



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>TKT1_KLULA (Q12630) Transketolase (EC 2.2.1.1) (TK)|
          Length = 679

 Score = 30.0 bits (66), Expect = 5.6
 Identities = 18/54 (33%), Positives = 30/54 (55%)
 Frame = -1

Query: 246 VSRLRIILSSEHSVCGLLFFFLQLWGIFHNLRFFSHFQGAGTNPCLYRPLFHLP 85
           ++R R +LS+ H+ C LL+  L L+G   ++    HF+  G+    + P F LP
Sbjct: 58  INRDRFVLSNGHA-CALLYSLLHLFGYDMSIEDLKHFRHLGSKTPGH-PEFELP 109



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>NUDH_ZYMMO (Q9RH11) Probable (di)nucleoside polyphosphate hydrolase (EC|
           3.6.1.-)
          Length = 155

 Score = 30.0 bits (66), Expect = 5.6
 Identities = 13/30 (43%), Positives = 20/30 (66%)
 Frame = -1

Query: 564 DLAEDVLQLAKNGLERRGYKEVGFLREVDE 475
           D+ E+  Q+ + GLE +   EVG LRE++E
Sbjct: 28  DMKEEAWQMPQGGLEAKETPEVGVLRELEE 57



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>IKAR_MOUSE (Q03267) DNA-binding protein Ikaros (Lymphoid transcription factor|
           LyF-1)
          Length = 517

 Score = 30.0 bits (66), Expect = 5.6
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = -1

Query: 324 GSSDCIPVQKSRPQFHKPPDEG 259
           GSS+ +PV  S  Q HKPP +G
Sbjct: 332 GSSEVVPVISSMYQLHKPPSDG 353



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>ORCT3_MOUSE (Q6A4L0) Organic cation transporter-like 3 (Solute carrier family|
           22 member 13)
          Length = 551

 Score = 29.6 bits (65), Expect = 7.3
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = -3

Query: 115 LFVPTPFSPSPDERLVFGINPSLSCDDGTSFPENNSE 5
           +F P P S S ++ L    N + +CD G  +PEN  +
Sbjct: 85  MFRPPPDSASLEDILSHRFNETQACDSGWDYPENRPQ 121



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>PCNT_MOUSE (P48725) Pericentrin|
          Length = 1920

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 19/70 (27%), Positives = 34/70 (48%), Gaps = 2/70 (2%)
 Frame = +1

Query: 40  HTTEMDLFRIQAVHQGKVKK--GSVQTGVCSGSLEMREKS*VVKDPPQLEKEKQQATNRV 213
           H  E+ L  ++A HQ  +KK    +Q+  C    ++ +K    +   +LE E  QA+   
Sbjct: 272 HEAEVSLKNLEAQHQAAIKKLQEDLQSEHCQYLQDLEQKFREKEKAKELELETLQASYED 331

Query: 214 FRAQNYSQPR 243
            +AQ+  + R
Sbjct: 332 LKAQSQEEIR 341



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>SYFB_PSEPK (Q88K22) Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)|
           (Phenylalanine--tRNA ligase beta chain) (PheRS)
          Length = 793

 Score = 29.3 bits (64), Expect = 9.6
 Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
 Frame = -3

Query: 451 LLRSF*IYTRRSGSAAWTPSSRSCYIDTLNHI*LRWTLDKVDRVFRLYPCTKIPSSIPQA 272
           LL +  + T +SG   WT S  S   D      +   +D ++ + RLY    +P   PQA
Sbjct: 429 LLNALELTTTKSGEGQWTVSVPSHRFD------ISLEVDLIEELARLYGYNNLPVRYPQA 482

Query: 271 --T*RGKP 254
               +GKP
Sbjct: 483 RLAPQGKP 490


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,709,064
Number of Sequences: 219361
Number of extensions: 2147523
Number of successful extensions: 6508
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 6250
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6505
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 5538924943
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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