ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | rbags1e12 |
|---|---|
| Clone Library Name | barley_pub |
>CAS1_ARATH (P38605) Cycloartenol synthase (EC 5.4.99.8)| (2,3-epoxysqualene--cycloartenol cyclase) Length = 759 Score = 204 bits (518), Expect = 3e-52 Identities = 99/177 (55%), Positives = 128/177 (72%), Gaps = 2/177 (1%) Frame = -3 Query: 717 RCKEIEKCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRK 538 R KE+++CI+ A +IE+ Q +GSWYG+W +CFTYG +F VKGL A G+T NS + K Sbjct: 581 RKKEVDECIEKAVKFIESIQAADGSWYGSWAVCFTYGTWFGVKGLVAVGKTLKNSPHVAK 640 Query: 537 SCEFLLSKQQSTGGWGESYLSIETEEY--VDSGSSHAVATAWAMLALIYAGQAEIYPVPL 364 +CEFLLSKQQ +GGWGESYLS + + Y +D SH V TAWAMLALI AGQAE+ PL Sbjct: 641 ACEFLLSKQQPSGGWGESYLSCQDKVYSNLDGNRSHVVNTAWAMLALIGAGQAEVDRKPL 700 Query: 363 YRAAKLLMNMQLDSGEFPQQEHMGCANSAFFFNYPNYRNIFPIMALGEFRRRLIENQ 193 +RAA+ L+N Q+++G+FPQQE MG N Y YRNIFPI ALGE+R +++ Q Sbjct: 701 HRAARYLINAQMENGDFPQQEIMGVFNRNCMITYAAYRNIFPIWALGEYRCQVLLQQ 757
>ERG7_YEAST (P38604) Lanosterol synthase (EC 5.4.99.7) (Oxidosqualene--lanosterol| cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC) Length = 730 Score = 149 bits (376), Expect = 8e-36 Identities = 79/171 (46%), Positives = 103/171 (60%), Gaps = 2/171 (1%) Frame = -3 Query: 717 RCKEIEKCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRK 538 R +EI I+ A +I+ Q +GSWYG+WGICFTY FA++ L G TY+NS +RK Sbjct: 557 RKEEIRTRIRIAIEFIKKSQLPDGSWYGSWGICFTYAGMFALEALHTVGETYENSSTVRK 616 Query: 537 SCEFLLSKQQSTGGWGESYLSIETEEYVDSGSSHAVATAWAMLALIYAGQAEIYP--VPL 364 C+FL+SKQ GGWGES S E YVDS S V TAWA++AL++A YP + Sbjct: 617 GCDFLVSKQMKDGGWGESMKSSELHSYVDSEKSLVVQTAWALIALLFAE----YPNKEVI 672 Query: 363 YRAAKLLMNMQLDSGEFPQQEHMGCANSAFFFNYPNYRNIFPIMALGEFRR 211 R LL N Q +SGE+ + G N + YP+YR +FPI ALG + R Sbjct: 673 DRGIDLLKNRQEESGEWKFESVEGVFNHSCAIEYPSYRFLFPIKALGMYSR 723
>ERG7_SCHPO (Q10231) Lanosterol synthase (EC 5.4.99.7) (Oxidosqualene--lanosterol| cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC) Length = 721 Score = 144 bits (363), Expect = 3e-34 Identities = 76/172 (44%), Positives = 99/172 (57%), Gaps = 2/172 (1%) Frame = -3 Query: 717 RCKEIEKCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRK 538 R EIE I+NA Y+ QR +GSWYG+W ICFTY A FA LA++GR Y+N +K Sbjct: 547 RRDEIENTIENALEYVVKMQRPDGSWYGSWAICFTYAAMFATGSLASAGRYYENCPVQKK 606 Query: 537 SCEFLLSKQQSTGGWGESYLSIETEEYVDSGSSHAVATAWAMLALIYAGQAEIYP--VPL 364 +CEFLLSKQ+ GGW ESY++ T Y ++ SS T WA+ ALI A YP P+ Sbjct: 607 ACEFLLSKQRPDGGWSESYMACVTGVYTETESSLVTQTGWALDALINAK----YPDRKPI 662 Query: 363 YRAAKLLMNMQLDSGEFPQQEHMGCANSAFFFNYPNYRNIFPIMALGEFRRR 208 + K LM Q G + Q+ G N YPNY+ F I LG+F ++ Sbjct: 663 EKGIKFLMASQKSDGSWQQKSMEGIFNKNVAIAYPNYKLYFSIYTLGKFAKQ 714
>ERG7_MOUSE (Q8BLN5) Lanosterol synthase (EC 5.4.99.7) (Oxidosqualene--lanosterol| cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC) Length = 733 Score = 136 bits (342), Expect = 7e-32 Identities = 67/177 (37%), Positives = 99/177 (55%), Gaps = 3/177 (1%) Frame = -3 Query: 717 RCKEIEKCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTY-DNSDC-- 547 R E+ + + + KQR +GSW G+WG+CFTYG +F ++ A G TY D + C Sbjct: 553 RAAEVRETLNQGLDFCRRKQRADGSWEGSWGVCFTYGTWFGLEAFACMGHTYQDGAACAE 612 Query: 546 MRKSCEFLLSKQQSTGGWGESYLSIETEEYVDSGSSHAVATAWAMLALIYAGQAEIYPVP 367 + ++C FLLS+Q + GGWGE + S E YV S S +T WA++ L+ +I Sbjct: 613 VAQACNFLLSQQMADGGWGEDFESCEQRRYVQSARSQVHSTCWALMGLMAVRHPDI--TA 670 Query: 366 LYRAAKLLMNMQLDSGEFPQQEHMGCANSAFFFNYPNYRNIFPIMALGEFRRRLIEN 196 R + L+ QL +G++PQ+ G N + +Y +YRNIFPI ALG F +N Sbjct: 671 QERGIRCLLGKQLPNGDWPQENISGVFNKSCAISYTSYRNIFPIWALGRFSNLYPDN 727
>ERG7_HUMAN (P48449) Lanosterol synthase (EC 5.4.99.7) (Oxidosqualene--lanosterol| cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC) Length = 732 Score = 135 bits (339), Expect = 2e-31 Identities = 66/170 (38%), Positives = 98/170 (57%), Gaps = 3/170 (1%) Frame = -3 Query: 717 RCKEIEKCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTY-DNSDC-- 547 R EI + + + +QR +GSW G+WG+CFTYG +F ++ A G+TY D + C Sbjct: 552 RAAEIRETLTQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAE 611 Query: 546 MRKSCEFLLSKQQSTGGWGESYLSIETEEYVDSGSSHAVATAWAMLALIYAGQAEIYPVP 367 + ++C+FLLS+Q + GGWGE + S E Y+ S S T WAM+ L+ +I Sbjct: 612 VSRACDFLLSRQMADGGWGEDFESCEERRYLQSAQSQIHNTCWAMMGLMAVRHPDIEAQE 671 Query: 366 LYRAAKLLMNMQLDSGEFPQQEHMGCANSAFFFNYPNYRNIFPIMALGEF 217 R + L+ QL +G++PQ+ G N + +Y +YRNIFPI ALG F Sbjct: 672 --RGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIFPIWALGRF 719
>ERG7_RAT (P48450) Lanosterol synthase (EC 5.4.99.7) (Oxidosqualene--lanosterol| cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC) Length = 733 Score = 135 bits (339), Expect = 2e-31 Identities = 66/170 (38%), Positives = 93/170 (54%), Gaps = 3/170 (1%) Frame = -3 Query: 717 RCKEIEKCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDC--- 547 R E + + + KQR +GSW G+WG+CFTYG +F ++ A G Y N Sbjct: 553 RATESRETLNQGLDFCRKKQRADGSWEGSWGVCFTYGTWFGLEAFACMGHIYQNRTACAE 612 Query: 546 MRKSCEFLLSKQQSTGGWGESYLSIETEEYVDSGSSHAVATAWAMLALIYAGQAEIYPVP 367 + ++C FLLS+Q + GGWGE + S E YV S S +T WA+L L+ +I Sbjct: 613 VAQACHFLLSRQMADGGWGEDFESCEQRRYVQSAGSQVHSTCWALLGLMAVRHPDISAQE 672 Query: 366 LYRAAKLLMNMQLDSGEFPQQEHMGCANSAFFFNYPNYRNIFPIMALGEF 217 R + L+ QL +G++PQ+ G N + +Y NYRNIFPI ALG F Sbjct: 673 --RGIRCLLGKQLPNGDWPQENISGVFNKSCAISYTNYRNIFPIWALGRF 720
>ERG7_CANAL (Q04782) Lanosterol synthase (EC 5.4.99.7) (Oxidosqualene--lanosterol| cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC) Length = 728 Score = 134 bits (338), Expect = 2e-31 Identities = 67/167 (40%), Positives = 99/167 (59%), Gaps = 1/167 (0%) Frame = -3 Query: 705 IEKCIKNAATYIENKQRK-NGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRKSCE 529 I+K I +A YI + Q +GSWYG WGIC+TY + FA++ L G Y++S ++K C+ Sbjct: 557 IQKTISSAIQYILDSQDNIDGSWYGCWGICYTYASMFALEALHTVGLDYESSSAVKKGCD 616 Query: 528 FLLSKQQSTGGWGESYLSIETEEYVDSGSSHAVATAWAMLALIYAGQAEIYPVPLYRAAK 349 FL+SKQ GGW ES ET YV+ +S V +AWA++ LI + P+ R + Sbjct: 617 FLISKQLPDGGWSESMKGCETHSYVNGENSLVVQSAWALIGLILGNYPD--EEPIKRGIQ 674 Query: 348 LLMNMQLDSGEFPQQEHMGCANSAFFFNYPNYRNIFPIMALGEFRRR 208 LM QL +GE+ ++ G N + YP+YR +FPI ALG ++ + Sbjct: 675 FLMKRQLPTGEWKYEDIEGVFNHSCAIEYPSYRFLFPIKALGLYKNK 721
>ERG7_PNECA (Q96WJ0) Lanosterol synthase (EC 5.4.99.7) (Oxidosqualene--lanosterol| cyclase) (2,3-epoxysqualene--lanosterol cyclase) (OSC) Length = 719 Score = 129 bits (324), Expect = 9e-30 Identities = 67/171 (39%), Positives = 96/171 (56%), Gaps = 1/171 (0%) Frame = -3 Query: 717 RCKEIEKCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRK 538 R EI K +KNA +I+ QR +GSWY +W ICFTY FA++ L+ Y+NS R+ Sbjct: 547 RGPEINKSVKNAIQFIKESQRPDGSWYESWAICFTYATMFALESLSCVKDFYENSFHSRR 606 Query: 537 SCEFLLSKQQSTGGWGESYLSIETEEYV-DSGSSHAVATAWAMLALIYAGQAEIYPVPLY 361 +C+FL++KQ+ GGW E Y S + S V TAWA + L+YA + P+ Sbjct: 607 ACDFLVNKQEEDGGWSEGYQSCTDGIWTRHPTGSQVVQTAWACIGLMYANYPD--ETPIK 664 Query: 360 RAAKLLMNMQLDSGEFPQQEHMGCANSAFFFNYPNYRNIFPIMALGEFRRR 208 R L+M+ Q +GE+ Q+ G N +YPNY+ F I ALG + +R Sbjct: 665 RGINLIMSRQQPNGEWKQEAIEGVFNKNCMISYPNYKFNFTIKALGMYSKR 715
>SQHC_ALIAC (P33247) Squalene--hopene cyclase (EC 5.4.99.17)| Length = 630 Score = 97.1 bits (240), Expect = 5e-20 Identities = 53/166 (31%), Positives = 85/166 (51%), Gaps = 1/166 (0%) Frame = -3 Query: 699 KCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRKSCEFLL 520 K I+ A Y++ +Q+ +GSW+G WG+ + YG V L A G ++K+ +++ Sbjct: 465 KVIRRAVEYLKREQKPDGSWFGRWGVNYLYGTGAVVSALKAVGID-TREPYIQKALDWVE 523 Query: 519 SKQQSTGGWGESYLSIETEEYVDSGSSHAVATAWAMLALIYAGQAEIYPVPLYRAAKLLM 340 Q GGWGE S E Y G+S TAWA++ALI G+AE R + L+ Sbjct: 524 QHQNPDGGWGEDCRSYEDPAYAGKGASTPSQTAWALMALIAGGRAE--SEAARRGVQYLV 581 Query: 339 NMQLDSGEFPQQEHMGCA-NSAFFFNYPNYRNIFPIMALGEFRRRL 205 Q G + + + G F+ Y YR++FP +ALG +++ + Sbjct: 582 ETQRPDGGWDEPYYTGTGFPGDFYLGYTMYRHVFPTLALGRYKQAI 627
>SQHC_RHISN (P55348) Probable squalene--hopene cyclase (EC 5.4.99.17)| Length = 647 Score = 91.3 bits (225), Expect = 3e-18 Identities = 55/165 (33%), Positives = 83/165 (50%), Gaps = 3/165 (1%) Frame = -3 Query: 699 KCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCM-RKSCEFL 523 K + Y+ Q GSWYG WG+ + YG + + L A+G D+ D M RK+ E+L Sbjct: 480 KAVAEGIAYLRRTQHAEGSWYGRWGLNYIYGTWSVLCALNAAG--IDHQDPMIRKAVEWL 537 Query: 522 LSKQQSTGGWGESYLSIETE-EYVDSGSSHAVATAWAMLALIYAGQAEIYPVPLYRAAKL 346 +S Q GGWGE +S + + S + TAWA+L L+ AG+ E +P + R Sbjct: 538 VSIQSWDGGWGEDAISYRLDYSGYEQAPSTSSQTAWALLGLMAAGEVE-HPA-VARGVNY 595 Query: 345 LMNMQLDSGEFPQQEHMGCA-NSAFFFNYPNYRNIFPIMALGEFR 214 L N Q ++G + +Q + F+ Y Y FP+ AL +R Sbjct: 596 LKNAQTENGLWDEQRYTATGFPRVFYLRYHGYSKFFPLWALARYR 640
>SQHC_BRAJA (P54924) Squalene--hopene cyclase (EC 5.4.99.17)| Length = 660 Score = 88.6 bits (218), Expect = 2e-17 Identities = 52/164 (31%), Positives = 85/164 (51%), Gaps = 2/164 (1%) Frame = -3 Query: 699 KCIKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRKSCEFLL 520 K + + Y+ Q GSWYG WG+ F YG + + L +G +D+ +RK+ ++L Sbjct: 486 KHVADGVAYLRKTQHPEGSWYGRWGMNFIYGTWSVLCALNMAGVRHDD-PMIRKAADWLA 544 Query: 519 SKQQSTGGWGESYLSIETE-EYVDSGSSHAVATAWAMLALIYAGQAEIYPVPLYRAAKLL 343 S Q GGWGE +S + + ++ S A TAWA+LAL+ AG+ + +P + R + L Sbjct: 545 SIQNKDGGWGEDAVSYRLDYKGWEAAPSTASQTAWALLALMAAGEVD-HPA-VARGVEYL 602 Query: 342 MNMQLDSGEFPQQEHMGCA-NSAFFFNYPNYRNIFPIMALGEFR 214 + Q + G + +Q + F+ Y Y FP+ AL +R Sbjct: 603 IATQNEKGLWDEQRYTATGFPRVFYLRYHGYSKFFPLWALARYR 646
>SQHC_ZYMMO (P33990) Squalene--hopene cyclase (EC 5.4.99.17)| Length = 658 Score = 76.3 bits (186), Expect = 9e-14 Identities = 53/172 (30%), Positives = 84/172 (48%), Gaps = 5/172 (2%) Frame = -3 Query: 693 IKNAATYIENKQRKNGSWYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRKSCEFLLSK 514 +K A Y+ +Q ++GSW+G WG+ + YG + A+ L + +D+ ++K+ +L + Sbjct: 486 MKAAVDYLLKEQEEDGSWFGRWGVNYIYGTWSALCALNVAALPHDHL-AIQKAVAWLKTI 544 Query: 513 QQSTGGWGE---SY-LSIETEEYVDSGSSHAVATAWAMLALIYAGQAEIYPVPLYRAAKL 346 Q GGWGE SY L E +DS +S TAWA+L L+ G+A V + Sbjct: 545 QNEDGGWGENCDSYALDYSGYEPMDSTASQ---TAWALLGLMAVGEANSEAVT--KGINW 599 Query: 345 LMNMQLDSGEFPQQEHMGCA-NSAFFFNYPNYRNIFPIMALGEFRRRLIENQ 193 L Q + G + + + G F+ Y Y FP+ AL +R NQ Sbjct: 600 LAQNQDEEGLWKEDYYSGGGFPRVFYLRYHGYSKYFPLWALARYRNLKKANQ 651
>UBP49_MOUSE (Q6P9L4) Ubiquitin carboxyl-terminal hydrolase 49 (EC 3.1.2.15)| (Ubiquitin thioesterase 49) (Ubiquitin-specific-processing protease 49) (Deubiquitinating enzyme 49) Length = 685 Score = 30.4 bits (67), Expect = 5.7 Identities = 15/53 (28%), Positives = 20/53 (37%) Frame = -3 Query: 642 WYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRKSCEFLLSKQQSTGGWGES 484 W G W + + +V L + R YD D CE L QQ G + Sbjct: 366 WSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGST 418
>UBP49_HUMAN (Q70CQ1) Ubiquitin carboxyl-terminal hydrolase 49 (EC 3.1.2.15)| (Ubiquitin thioesterase 49) (Ubiquitin-specific-processing protease 49) (Deubiquitinating enzyme 49) Length = 688 Score = 30.0 bits (66), Expect = 7.5 Identities = 14/45 (31%), Positives = 18/45 (40%) Frame = -3 Query: 642 WYGTWGICFTYGAFFAVKGLAASGRTYDNSDCMRKSCEFLLSKQQ 508 W G W + + +V L + R YD D CE L QQ Sbjct: 369 WSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQ 413 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 101,459,437 Number of Sequences: 219361 Number of extensions: 2102250 Number of successful extensions: 5522 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 5348 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5494 length of database: 80,573,946 effective HSP length: 109 effective length of database: 56,663,597 effective search space used: 7366267610 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
