ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | FLbaf141m19 |
|---|---|
| Clone Library Name | barley_pub |
>LOC_Os09g32274:12009.t02843:unspliced-genomic 30S ribosomal protein S16, putative, expressed| Length = 1717 Score = 101 bits (216), Expect = 3e-21 Identities = 41/45 (91%), Positives = 42/45 (93%) Frame = +3 / +3 Query: 177 TMVVRIRLTRFGCRNRPFYRVMAADSRWLRDGKHLEVLGYYNPLP 311 +MVVRIRL RFGCRNRPFYRVMAADSR RDGKHLEVLGYYNPLP Sbjct: 108 SMVVRIRLARFGCRNRPFYRVMAADSRSPRDGKHLEVLGYYNPLP 242 Score = 89.0 bits (188), Expect(2) = 3e-18 Identities = 39/45 (86%), Positives = 41/45 (91%) Frame = -1 / -1 Query: 310 GSGL**PRTSRCLPSRSQRLSAAITR*KGRLRQPNRVSRIRTTMV 176 GSGL**PRTSRCLPSR +RLSAAITR* GR RQPNR SRIRTTM+ Sbjct: 241 GSGL**PRTSRCLPSRGERLSAAITR*NGRFRQPNRASRIRTTML 107 Score = 22.1 bits (42), Expect(2) = 3e-18 Identities = 7/7 (100%), Positives = 7/7 (100%) Frame = -2 / -2 Query: 315 HRGAGCS 295 HRGAGCS Sbjct: 246 HRGAGCS 226 Score = 89.0 bits (188), Expect = 2e-17 Identities = 36/44 (81%), Positives = 39/44 (88%) Frame = -3 / -3 Query: 311 GERVVVAQDLEVLAVAQPAAVGSHHPVEGPVAAAEPREPDPDHH 180 GERVVVA+DLEVLAVA+ AAVG HHPVE PV AAEPR+PDP HH Sbjct: 242 GERVVVAEDLEVLAVARGAAVGGHHPVERPVPAAEPRQPDPHHH 111 Score = 40.5 bits (82), Expect = 0.010 Identities = 15/18 (83%), Positives = 17/18 (94%) Frame = +2 / +2 Query: 191 DPAHAVRLPQPALLPGDG 244 DPA AVRLP+PA+LPGDG Sbjct: 122 DPAGAVRLPEPAVLPGDG 175 Score = 35.9 bits (72), Expect = 0.23 Identities = 15/19 (78%), Positives = 16/19 (84%) Frame = -2 / -2 Query: 246 QPSPGRRAGCGSRTA*AGS 190 +PSPGR AG GSRTA AGS Sbjct: 177 RPSPGRTAGSGSRTAPAGS 121
>LOC_Os08g40610:12008.t03791:unspliced-genomic 30S ribosomal protein S16, putative, expressed| Length = 1729 Score = 97.7 bits (207), Expect = 6e-20 Identities = 39/44 (88%), Positives = 41/44 (93%) Frame = +3 / +3 Query: 180 MVVRIRLTRFGCRNRPFYRVMAADSRWLRDGKHLEVLGYYNPLP 311 MVVRIRL RFGCRNRPFYR+MAADSR RDGKHLEVLGYY+PLP Sbjct: 90 MVVRIRLARFGCRNRPFYRLMAADSRSPRDGKHLEVLGYYDPLP 221 Score = 89.0 bits (188), Expect = 2e-17 Identities = 36/44 (81%), Positives = 40/44 (90%) Frame = -3 / -3 Query: 311 GERVVVAQDLEVLAVAQPAAVGSHHPVEGPVAAAEPREPDPDHH 180 GERVVVA+DLEVLAVA+ AAVG H PVEGPV AAEPR+PDPD+H Sbjct: 221 GERVVVAEDLEVLAVARGAAVGGHEPVEGPVPAAEPRQPDPDNH 90 Score = 76.7 bits (161), Expect = 1e-13 Identities = 34/44 (77%), Positives = 38/44 (86%) Frame = -1 / -1 Query: 310 GSGL**PRTSRCLPSRSQRLSAAITR*KGRLRQPNRVSRIRTTM 179 GSG **PRTSRCLPSR +RLSAA++R*KG RQPNR S IRTT+ Sbjct: 220 GSGS**PRTSRCLPSRGERLSAAMSR*KGLFRQPNRASLIRTTI 89 Score = 31.8 bits (63), Expect = 4.1 Identities = 13/19 (68%), Positives = 14/19 (73%) Frame = +2 / +2 Query: 188 PDPAHAVRLPQPALLPGDG 244 PD A AVRLP+ ALLP G Sbjct: 98 PDQAGAVRLPEQALLPAHG 154
>LOC_Os11g13930:12011.t01227:unspliced-genomic zinc finger homeodomain protein 1, putative, expressed| Length = 2040 Score = 30.9 bits (61), Expect(2) = 0.084 Identities = 11/23 (47%), Positives = 12/23 (52%) Frame = -3 / +2 Query: 248 GSHHPVEGPVAAAEPREPDPDHH 180 G+ P AAA P P PDHH Sbjct: 905 GARAPATAAAAAARPPPPPPDHH 973 Score = 24.0 bits (46), Expect(2) = 0.084 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = -3 / +3 Query: 287 DLEVLAVAQPAAVGSHHPVEGP 222 D + A A AA G HH + GP Sbjct: 759 DADSCAAALRAAAGRHHHLLGP 824
>LOC_Os08g04550:12008.t00349:unspliced-genomic hypothetical protein| Length = 819 Score = 36.3 bits (73), Expect = 0.17 Identities = 16/37 (43%), Positives = 22/37 (59%) Frame = +3 / -3 Query: 21 HSALLQILSRLPRLPPTIPTAADLVSHPRFQHPHPSP 131 H+ LL++ +RLP P P+ A S RF HPH +P Sbjct: 454 HAPLLRLRARLPPSAPWPPSDALPDSRVRFHHPHRAP 344
>LOC_Os12g17880:12012.t01639:unspliced-genomic ubiquitin-protein ligase, putative, expressed| Length = 3379 Score = 35.4 bits (71), Expect = 0.32 Identities = 15/32 (46%), Positives = 17/32 (53%) Frame = -3 / -3 Query: 272 AVAQPAAVGSHHPVEGPVAAAEPREPDPDHHG 177 AV Q HHPV A A+PR+ P HHG Sbjct: 704 AVPQHGGHHGHHPVAAHDALADPRKLPPAHHG 609
>LOC_Os07g19190:12007.t01775:unspliced-genomic ribosome biogenesis protein RLP24, putative, expressed| Length = 3128 Score = 35.4 bits (71), Expect = 0.32 Identities = 14/24 (58%), Positives = 16/24 (66%) Frame = +3 / +2 Query: 54 PRLPPTIPTAADLVSHPRFQHPHP 125 P LPPT P +DL+S PR PHP Sbjct: 2 PFLPPTPPLWSDLLSTPRRPRPHP 73
>LOC_Os06g48590:12006.t04545:unspliced-genomic OsMPK7 - putative MAPK based on amino acid sequence homology,| expressed Length = 4425 Score = 34.1 bits (68), Expect = 0.83 Identities = 13/26 (50%), Positives = 14/26 (53%) Frame = +3 / -3 Query: 54 PRLPPTIPTAADLVSHPRFQHPHPSP 131 P PPT PT D + PR HP SP Sbjct: 505 PPPPPTTPTPTDRIGSPRKAHPPTSP 428
>LOC_Os03g33012:12003.t02889:unspliced-genomic WRKY transcription factor 4, putative, expressed| Length = 10786 Score = 34.1 bits (68), Expect = 0.83 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = -3 / -3 Query: 323 KANIGERVVVAQDLEVLAVAQPAAVGSHHPVEGP 222 K N+ R+ + +D +L A+GSHHP + P Sbjct: 7778 KCNLKNRITLGEDSLLLLFDHTCAIGSHHPKDQP 7677
>LOC_Os09g02490:12009.t00148:unspliced-genomic transposon protein, putative, CACTA, En/Spm sub-class| Length = 2686 Score = 32.7 bits (65), Expect = 2.1 Identities = 14/39 (35%), Positives = 19/39 (48%) Frame = +3 / -2 Query: 9 LTTHHSALLQILSRLPRLPPTIPTAADLVSHPRFQHPHP 125 LTT H +L +++ P IPT+ L HPHP Sbjct: 552 LTTFHQSLQSSITKFINAPHFIPTSTCLTHSSITYHPHP 436
>LOC_Os08g18070:12008.t01677:unspliced-genomic hypothetical protein| Length = 2088 Score = 32.7 bits (65), Expect = 2.1 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = +3 / -1 Query: 45 SRLPRLPPTIPTAADLVSHPRFQHPHP 125 +R+P LPP++P AAD+ +H P P Sbjct: 837 ARMPALPPSLPQAADVRAHLSAPPPPP 757
>LOC_Os08g13150:12008.t01193:unspliced-genomic transposon protein, putative, CACTA, En/Spm sub-class| Length = 6189 Score = 32.7 bits (65), Expect = 2.1 Identities = 14/39 (35%), Positives = 19/39 (48%) Frame = +3 / -1 Query: 9 LTTHHSALLQILSRLPRLPPTIPTAADLVSHPRFQHPHP 125 LTT H +L +++ P IPT+ L HPHP Sbjct: 3762 LTTFHQSLQSSITKFINAPHFIPTSTCLTHSSITYHPHP 3646
>LOC_Os06g39560:12006.t03653:unspliced-genomic hypothetical protein| Length = 665 Score = 26.7 bits (52), Expect(2) = 2.7 Identities = 10/20 (50%), Positives = 10/20 (50%) Frame = +2 / -2 Query: 188 PDPAHAVRLPQPALLPGDGC 247 P PA RLP P P GC Sbjct: 211 PTPAQPRRLPPPPSFPAAGC 152 Score = 24.4 bits (47), Expect(2) = 2.7 Identities = 11/30 (36%), Positives = 13/30 (43%) Frame = +3 / -3 Query: 117 PHPSPXXXXXXXXXXXXXSITMVVRIRLTR 206 PHPSP S T+ R+R TR Sbjct: 552 PHPSPPPCLLLRAFPAAASTTVASRLRQTR 463
>LOC_Os02g06330:12002.t00531:unspliced-genomic ethylene-responsive transcription factor 3, putative, expressed| Length = 1135 Score = 32.2 bits (64), Expect = 3.0 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = +3 / -2 Query: 30 LLQILSRLPRLPPTIPTAADLVSHPRFQHPHP 125 LL+ + L R+PP P V H F HPHP Sbjct: 102 LLRRVPDLGRVPPRRPPPHPTVPHLPFPHPHP 7
>LOC_Os07g40224:12007.t004656:unspliced-genomic expressed protein| Length = 2462 Score = 32.2 bits (64), Expect = 3.0 Identities = 13/19 (68%), Positives = 14/19 (73%) Frame = -2 / -3 Query: 57 EGDEIGSGEVQSGGWLGFP 1 EGDEIGSGE + GG G P Sbjct: 120 EGDEIGSGEEEEGGGGGAP 64
>LOC_Os07g22670:12007.t01970:unspliced-genomic mTERF family protein, expressed| Length = 2044 Score = 26.3 bits (51), Expect(2) = 3.0 Identities = 12/25 (48%), Positives = 12/25 (48%) Frame = +3 / +2 Query: 57 RLPPTIPTAADLVSHPRFQHPHPSP 131 RLP P A L H RF P P P Sbjct: 221 RLPRRPPRCAQLPPHLRFPLPPPPP 295 Score = 23.1 bits (44), Expect(2) = 3.0 Identities = 9/17 (52%), Positives = 11/17 (64%) Frame = +3 / +2 Query: 18 HHSALLQILSRLPRLPP 68 H AL + +RLPR PP Sbjct: 191 HRRALPALPARLPRRPP 241
>LOC_Os12g23790:12012.t02109:unspliced-genomic hypothetical protein| Length = 4253 Score = 31.8 bits (63), Expect = 4.1 Identities = 13/38 (34%), Positives = 18/38 (47%) Frame = +3 / -1 Query: 12 TTHHSALLQILSRLPRLPPTIPTAADLVSHPRFQHPHP 125 TT H +L +++ P IPT+ L HPHP Sbjct: 788 TTFHQSLQSSITKFKNAPHFIPTSTCLTHSSITYHPHP 675
>LOC_Os07g01390:12007.t00038:unspliced-genomic transposon protein, putative, CACTA, En/Spm sub-class| Length = 6273 Score = 31.8 bits (63), Expect = 4.1 Identities = 14/39 (35%), Positives = 18/39 (46%) Frame = +3 / -3 Query: 9 LTTHHSALLQILSRLPRLPPTIPTAADLVSHPRFQHPHP 125 LTT H L +++ P IPT+ L HPHP Sbjct: 3769 LTTFHQPLQSSITKFINAPHFIPTSTCLTHSSITYHPHP 3653
>LOC_Os12g01010:12012.t00001:unspliced-genomic RNA and export factor-binding protein 2, putative, expressed| Length = 2747 Score = 31.8 bits (63), Expect = 4.1 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = -2 / +3 Query: 564 FFFFLSNNHYNLNICHIMNTIKHIQTWHISIKHG*HQVYNQN*FTL 427 F FF N+YNLN ++ + IK + H+ I H + N F L Sbjct: 456 FSFFPKKNYYNLNSPYLDSCIKLLLNCHLYIPQFPHLFISPNQFCL 593
>LOC_Os08g40350:12008.t03766:unspliced-genomic expressed protein| Length = 4697 Score = 25.8 bits (50), Expect(2) = 5.1 Identities = 12/21 (57%), Positives = 13/21 (61%) Frame = -2 / +2 Query: 531 LNICHIMNTIKHIQTWHISIK 469 L I I TIKHI+T H S K Sbjct: 1232 LGIKDIAATIKHIETQHASSK 1294 Score = 22.6 bits (43), Expect(2) = 5.1 Identities = 8/17 (47%), Positives = 9/17 (52%) Frame = -2 / +1 Query: 567 YFFFFLSNNHYNLNICH 517 YF +S N Y L CH Sbjct: 1060 YFIVSISENAYMLRKCH 1110
>LOC_Os08g38190:12008.t03555:unspliced-genomic hypothetical protein| Length = 4143 Score = 25.8 bits (50), Expect(2) = 5.1 Identities = 12/21 (57%), Positives = 13/21 (61%) Frame = -2 / +1 Query: 531 LNICHIMNTIKHIQTWHISIK 469 L I I TIKHI+T H S K Sbjct: 820 LGIKDIAATIKHIETQHASSK 882 Score = 22.6 bits (43), Expect(2) = 5.1 Identities = 8/17 (47%), Positives = 9/17 (52%) Frame = -2 / +3 Query: 567 YFFFFLSNNHYNLNICH 517 YF +S N Y L CH Sbjct: 648 YFIVSISENAYMLRKCH 698
>LOC_Os09g10580:12009.t00850:unspliced-genomic hypothetical protein| Length = 1180 Score = 31.3 bits (62), Expect = 5.6 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +3 / +3 Query: 39 ILSRLPRLPPTIPTAADLVSHPRFQHPHP 125 + S LP PP++P + +HP +PHP Sbjct: 1029 LTSSLPSSPPSLPLS*IPYAHPTHPYPHP 1115
>LOC_Os07g30880:12007.t02775:unspliced-genomic retrotransposon protein, putative, Ty3-gypsy subclass| Length = 6723 Score = 31.3 bits (62), Expect = 5.6 Identities = 10/23 (43%), Positives = 12/23 (52%) Frame = +3 / +3 Query: 63 PPTIPTAADLVSHPRFQHPHPSP 131 PP PT+ H R+ HP P P Sbjct: 2286 PPRQPTSTTTTRHQRYSHPDPRP 2354
>LOC_Os06g23460:12006.t02158:unspliced-genomic nucleic acid binding protein, putative, expressed| Length = 7374 Score = 31.3 bits (62), Expect = 5.6 Identities = 14/32 (43%), Positives = 15/32 (46%) Frame = +3 / +2 Query: 24 SALLQILSRLPRLPPTIPTAADLVSHPRFQHP 119 S L SR +LPP IP S P F HP Sbjct: 4871 SILANRRSRSTKLPPMIPAQCGSTSRPTFLHP 4966
>LOC_Os05g41710:12005.t03708:unspliced-genomic retrotransposon protein, putative, unclassified| Length = 459 Score = 31.3 bits (62), Expect = 5.6 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = -3 / -3 Query: 266 AQPAAVGSHHPVEGPVAAAEPREPDPDH 183 A+ AV +H + P ++ PREP PDH Sbjct: 142 ARALAVVAHCQPQAPASSGCPREPPPDH 59
>LOC_Os04g25550:12004.t02262:unspliced-genomic cell division control protein 68, putative, expressed| Length = 5857 Score = 31.3 bits (62), Expect = 5.6 Identities = 14/40 (35%), Positives = 18/40 (45%) Frame = +3 / -2 Query: 12 TTHHSALLQILSRLPRLPPTIPTAADLVSHPRFQHPHPSP 131 T HHS L L +P P + P+ + R HPH P Sbjct: 5280 TPHHSPCLHCLPPVPAPPRSYPSLPQSLLQNRPHHPHLIP 5161
>LOC_Os01g58160:12001.t05214:unspliced-genomic hypothetical protein| Length = 2124 Score = 31.3 bits (62), Expect = 5.6 Identities = 11/18 (61%), Positives = 13/18 (72%) Frame = -2 / +3 Query: 63 GDEGDEIGSGEVQSGGWL 10 GD G + G GEV +GGWL Sbjct: 3 GDGGGDGGGGEVDNGGWL 56
>LOC_Os04g13364:12004.t01157:unspliced-genomic transposon protein, putative, CACTA, En/Spm sub-class, expressed| Length = 12290 Score = 24.9 bits (48), Expect(2) = 6.3 Identities = 14/31 (45%), Positives = 16/31 (51%) Frame = +3 / -1 Query: 39 ILSRLPRLPPTIPTAADLVSHPRFQHPHPSP 131 +LS L R P+ AADLV PR P P Sbjct: 5546 LLSLLFRWRPSGRRAADLVQPPRHHLAPPPP 5454 Score = 23.1 bits (44), Expect(2) = 6.3 Identities = 9/20 (45%), Positives = 9/20 (45%) Frame = +2 / -2 Query: 188 PDPAHAVRLPQPALLPGDGC 247 P P H LP LLP C Sbjct: 5458 PPPRHLALLPAALLLPPPPC 5399
>LOC_Os06g08790:12006.t00758:unspliced-genomic origin recognition complex subunit 1, putative, expressed| Length = 7754 Score = 30.4 bits (60), Expect(2) = 7.1 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 / +2 Query: 15 THHSALLQILSRLPRLPPTIPTAADLVSH 101 TH S L + +PR PP +P ++ LV H Sbjct: 1910 THQSHALAAVPTIPRPPPLLPVSSLLVLH 1996 Score = 22.6 bits (43), Expect(2) = 7.1 Identities = 7/12 (58%), Positives = 9/12 (75%) Frame = +2 / +3 Query: 314 CWPSFMFRRKVQ 349 CWPSF+ +K Q Sbjct: 4998 CWPSFVSIKKKQ 5033
>LOC_Os01g72170:12001.t06506:unspliced-genomic glutathione S-transferase, putative, expressed| Length = 1034 Score = 27.2 bits (53), Expect(2) = 7.3 Identities = 11/18 (61%), Positives = 12/18 (66%) Frame = -2 / +3 Query: 72 SLVGDEGDEIGSGEVQSG 19 +L GDEGD G GEV G Sbjct: 864 TLHGDEGDVHGDGEVNGG 917 Score = 23.1 bits (44), Expect(2) = 7.3 Identities = 10/23 (43%), Positives = 11/23 (47%) Frame = -3 / +3 Query: 263 QPAAVGSHHPVEGPVAAAEPREP 195 +PAA HP EGP R P Sbjct: 342 RPAAHPQPHPQEGPRPPPRRRRP 410
>LOC_Os11g31390:12011.t02737:unspliced-genomic hypothetical protein| Length = 4230 Score = 30.9 bits (61), Expect = 7.7 Identities = 14/30 (46%), Positives = 15/30 (50%) Frame = +3 / -2 Query: 15 THHSALLQILSRLPRLPPTIPTAADLVSHP 104 +H L QIL RLP LPP PT P Sbjct: 1229 SHIYPLAQILRRLPSLPPPPPTVTTTAPAP 1140
>LOC_Os10g40590:12010.t03299:unspliced-genomic oxysterol-binding protein 1, putative, expressed| Length = 5437 Score = 30.9 bits (61), Expect = 7.7 Identities = 11/23 (47%), Positives = 13/23 (56%) Frame = +3 / -3 Query: 48 RLPRLPPTIPTAADLVSHPRFQH 116 R P PP +P LV HPR +H Sbjct: 413 REPPRPPPLPDVGPLVQHPRHRH 345
>LOC_Os04g02820:12004.t00176:unspliced-genomic elongation factor 2, putative, expressed| Length = 5328 Score = 30.9 bits (61), Expect = 7.7 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = +3 / +2 Query: 18 HHSALLQILSRLPRLPPTIPTAADLVSHPR 107 HH A + + P LPP+IPT HPR Sbjct: 134 HHHAARCLRPQPPPLPPSIPTRTAHCFHPR 223
>LOC_Os02g51200:12002.t04704:unspliced-genomic retrotransposon protein, putative, unclassified| Length = 5897 Score = 30.9 bits (61), Expect = 7.7 Identities = 10/15 (66%), Positives = 11/15 (73%) Frame = -3 / -3 Query: 110 ESWVGDEVSGSGNRW 66 E W+GD V GSG RW Sbjct: 1914 EDWMGDGVGGSGARW 1870
>LOC_Os06g15020:12006.t01376:unspliced-genomic 3-ketoacyl-CoA synthase, putative, expressed| Length = 1754 Score = 30.9 bits (61), Expect = 7.7 Identities = 12/19 (63%), Positives = 15/19 (78%) Frame = +1 / -2 Query: 40 SYLVSLVSHQRFPLPLTSS 96 S+ VSL SHQR P+PL S+ Sbjct: 1525 SHHVSLTSHQRIPIPLLSA 1469
>LOC_Os05g45900:12005.t04078:unspliced-genomic inositol or phosphatidylinositol phosphatase/ inositol-polyphosphate| 5-phosphatase, putative, expressed Length = 5129 Score = 30.9 bits (61), Expect = 7.7 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = -2 / -1 Query: 540 HYNLNICHIMNTIKHIQTWHI 478 H I HI N++KHIQ +HI Sbjct: 2006 HVPTQILHIENSLKHIQKYHI 1944
>LOC_Os01g02040:12001.t00098:unspliced-genomic ATP binding protein, putative, expressed| Length = 3994 Score = 25.4 bits (49), Expect(2) = 9.2 Identities = 12/28 (42%), Positives = 13/28 (46%) Frame = +3 / +1 Query: 48 RLPRLPPTIPTAADLVSHPRFQHPHPSP 131 RLP P +P PR QHP SP Sbjct: 724 RLPPRAPQLPPPPPPRLPPRPQHPRRSP 807 Score = 22.1 bits (42), Expect(2) = 9.2 Identities = 8/16 (50%), Positives = 10/16 (62%) Frame = +2 / +3 Query: 188 PDPAHAVRLPQPALLP 235 P PA V +P PA +P Sbjct: 909 PPPAPPVAVPPPATVP 956 Database: tigr.seq.out Posted date: Jul 20, 2007 8:58 AM Number of letters in database: 166,841,660 Number of sequences in database: 56,278 Lambda K H 0.318 0.134 0.401 Matrix: BLOSUM62 Number of Sequences: 56278 Number of Hits to DB: 194,472,014 Number of extensions: 2888246 Number of successful extensions: 14821 Number of sequences better than 10.0: 36 Length of query: 189 Length of database: 55,613,886 Length adjustment: 48 Effective length of query: 141 Effective length of database: 52,912,542 Effective search space: 7460668422 Effective search space used: 7460668422 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 16 ( 7.3 bits)
