Clone Information

BLAST Search Result

Clone Name	FLbaf110o02
Clone Library Name	barley_pub

No.	Definition	Score (bits)	E Value
1	AK100592:Os12g0448900	1162	0.0
2	AF229813:Os12g0448900	1162	0.0
3	AK106731:Os01g0619800	32	2.6
4	Os04g0622000:Os04g0622000	30	7.5
5	AK102859:Os08g0528700	30	7.5
6	AK066974:Os02g0600200	30	9.8

>AK100592:Os12g0448900 |
          Length = 618
 Score = 1162 bits (3006), Expect = 0.0
 Identities = 567/618 (91%), Positives = 588/618 (95%), Gaps = 1/618 (0%)
 Frame = +3

Query: 93   MGSGLFKPRVHPDLRDVFSKMSFGDKIGFLFIHAFDKRNLWHKMPVPIGLLYLNTRRTLL 272
            MGSGLFKPRVHPDLRDVFSKMSF DKIGFLFIHAFDKRNLWHK+PVPIGLLYLNTRRTLL
Sbjct: 1    MGSGLFKPRVHPDLRDVFSKMSFFDKIGFLFIHAFDKRNLWHKVPVPIGLLYLNTRRTLL 60

Query: 273  EKYNLLAVG-SSHGAPFDPKEFPYRTGDGKYNDPHNAEAGSQYTFFGRNMKPVDQQDELM 449
            EKYNLLAVG SSHGA FDPKEF YRT DGKYNDPHNAEAGSQ TFFGRNM+PVDQQDELM
Sbjct: 61   EKYNLLAVGRSSHGALFDPKEFLYRTEDGKYNDPHNAEAGSQNTFFGRNMEPVDQQDELM 120

Query: 450  SPDPFVVATKLLARREYKDTGKQFNILAAAWIQFMVHDWMDHMEDTKQIEITAPKEVANE 629
            SPDPFVVATKLLARREYKDTGKQFNILAAAWIQFMVHDWMDHMEDT QI ITAPKEVANE
Sbjct: 121  SPDPFVVATKLLARREYKDTGKQFNILAAAWIQFMVHDWMDHMEDTGQIGITAPKEVANE 180

Query: 630  CPLKSFKFYATKEQPTNSDGIKTGYHNIRTAWWDGSAVYGNNEKQEKKIRTYADGKLVIG 809
            CPLKSFKF+ TKE PTNSDGIK G++NIRTAWWDGSAVYGNNE++ +K+RTY DGKLVIG
Sbjct: 181  CPLKSFKFHPTKELPTNSDGIKIGHYNIRTAWWDGSAVYGNNEERAEKLRTYVDGKLVIG 240

Query: 810  DDGLLLHEENGVPLSGDVRNGWVGISILQALFVKEHNAVCDAIKEEHPNLSDEELYRYAK 989
            DDGLLLH+ENGV LSGD+RN W G+SILQALFVKEHNAVCDAIKEEHPNLSDEELYRYAK
Sbjct: 241  DDGLLLHKENGVALSGDIRNSWAGVSILQALFVKEHNAVCDAIKEEHPNLSDEELYRYAK 300

Query: 990  LVTSAVIAKIHTIDWTVELLKTKTLRAGMRANWYGLLGKKIKDTFGHIGGTALGGLVGLK 1169
            LVTSAVIAK+HTIDWTVELLKTKT+RA MRANWYGLLGKKIKDTFGHIGG  LGGLVGLK
Sbjct: 301  LVTSAVIAKVHTIDWTVELLKTKTMRAAMRANWYGLLGKKIKDTFGHIGGPILGGLVGLK 360

Query: 1170 KPINHGVPYSLTEEFTSVYRMHPLIPSTLKLRDPTGQPAADNSPPYLEDIDIGELVGLKG 1349
            KP NHGVPYSLTEEFTSVYRMH LIPSTLKLRDPTGQP A+NSPP LEDIDIGE++GLKG
Sbjct: 361  KPNNHGVPYSLTEEFTSVYRMHSLIPSTLKLRDPTGQPDANNSPPCLEDIDIGEMIGLKG 420

Query: 1350 EDQLSKIGFEKQTLSMGYQACGALELWNYPSFFRDLIPQNLDGTNRSDRIDLAALEVYRD 1529
            E+QLSKIGFEKQ LSMGYQACGALELWNYPSFFR+LIPQNLDGTNRSDRIDLAALEVYRD
Sbjct: 421  EEQLSKIGFEKQALSMGYQACGALELWNYPSFFRNLIPQNLDGTNRSDRIDLAALEVYRD 480

Query: 1530 RERSVPRYNEFRRRLFLIPIKCWEDLTSDNDAIEAIRAIYGDDVEKLDLLVGLLAEKKIK 1709
            RERSVPRYNEFRRRLFLIPIK WEDLTSD DAIE IRAIYGDDVEKLDLLVGL+AEKKIK
Sbjct: 481  RERSVPRYNEFRRRLFLIPIKSWEDLTSDKDAIETIRAIYGDDVEKLDLLVGLMAEKKIK 540

Query: 1710 GFAISETAFNIFILMASRRLEADRFLTSNFNEKTYTKKGMQWVKTTEGLRDVINRHYPEI 1889
            GFAISETAFNIFILMASRRLEADRF TSNFNE+TYTKKGMQWVKTTEGLRDVINRHYPEI
Sbjct: 541  GFAISETAFNIFILMASRRLEADRFFTSNFNEETYTKKGMQWVKTTEGLRDVINRHYPEI 600

Query: 1890 TTNWMKSSSAFSVWDADY 1943
            T  WMKSSSAFSVWDADY
Sbjct: 601  TAKWMKSSSAFSVWDADY 618

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>AF229813:Os12g0448900 |
          Length = 618

 Score = 1162 bits (3006), Expect = 0.0
 Identities = 567/618 (91%), Positives = 588/618 (95%), Gaps = 1/618 (0%)
 Frame = +3

Query: 93   MGSGLFKPRVHPDLRDVFSKMSFGDKIGFLFIHAFDKRNLWHKMPVPIGLLYLNTRRTLL 272
            MGSGLFKPRVHPDLRDVFSKMSF DKIGFLFIHAFDKRNLWHK+PVPIGLLYLNTRRTLL
Sbjct: 1    MGSGLFKPRVHPDLRDVFSKMSFFDKIGFLFIHAFDKRNLWHKVPVPIGLLYLNTRRTLL 60

Query: 273  EKYNLLAVG-SSHGAPFDPKEFPYRTGDGKYNDPHNAEAGSQYTFFGRNMKPVDQQDELM 449
            EKYNLLAVG SSHGA FDPKEF YRT DGKYNDPHNAEAGSQ TFFGRNM+PVDQQDELM
Sbjct: 61   EKYNLLAVGRSSHGALFDPKEFLYRTEDGKYNDPHNAEAGSQNTFFGRNMEPVDQQDELM 120

Query: 450  SPDPFVVATKLLARREYKDTGKQFNILAAAWIQFMVHDWMDHMEDTKQIEITAPKEVANE 629
            SPDPFVVATKLLARREYKDTGKQFNILAAAWIQFMVHDWMDHMEDT QI ITAPKEVANE
Sbjct: 121  SPDPFVVATKLLARREYKDTGKQFNILAAAWIQFMVHDWMDHMEDTGQIGITAPKEVANE 180

Query: 630  CPLKSFKFYATKEQPTNSDGIKTGYHNIRTAWWDGSAVYGNNEKQEKKIRTYADGKLVIG 809
            CPLKSFKF+ TKE PTNSDGIK G++NIRTAWWDGSAVYGNNE++ +K+RTY DGKLVIG
Sbjct: 181  CPLKSFKFHPTKELPTNSDGIKIGHYNIRTAWWDGSAVYGNNEERAEKLRTYVDGKLVIG 240

Query: 810  DDGLLLHEENGVPLSGDVRNGWVGISILQALFVKEHNAVCDAIKEEHPNLSDEELYRYAK 989
            DDGLLLH+ENGV LSGD+RN W G+SILQALFVKEHNAVCDAIKEEHPNLSDEELYRYAK
Sbjct: 241  DDGLLLHKENGVALSGDIRNSWAGVSILQALFVKEHNAVCDAIKEEHPNLSDEELYRYAK 300

Query: 990  LVTSAVIAKIHTIDWTVELLKTKTLRAGMRANWYGLLGKKIKDTFGHIGGTALGGLVGLK 1169
            LVTSAVIAK+HTIDWTVELLKTKT+RA MRANWYGLLGKKIKDTFGHIGG  LGGLVGLK
Sbjct: 301  LVTSAVIAKVHTIDWTVELLKTKTMRAAMRANWYGLLGKKIKDTFGHIGGPILGGLVGLK 360

Query: 1170 KPINHGVPYSLTEEFTSVYRMHPLIPSTLKLRDPTGQPAADNSPPYLEDIDIGELVGLKG 1349
            KP NHGVPYSLTEEFTSVYRMH LIPSTLKLRDPTGQP A+NSPP LEDIDIGE++GLKG
Sbjct: 361  KPNNHGVPYSLTEEFTSVYRMHSLIPSTLKLRDPTGQPDANNSPPCLEDIDIGEMIGLKG 420

Query: 1350 EDQLSKIGFEKQTLSMGYQACGALELWNYPSFFRDLIPQNLDGTNRSDRIDLAALEVYRD 1529
            E+QLSKIGFEKQ LSMGYQACGALELWNYPSFFR+LIPQNLDGTNRSDRIDLAALEVYRD
Sbjct: 421  EEQLSKIGFEKQALSMGYQACGALELWNYPSFFRNLIPQNLDGTNRSDRIDLAALEVYRD 480

Query: 1530 RERSVPRYNEFRRRLFLIPIKCWEDLTSDNDAIEAIRAIYGDDVEKLDLLVGLLAEKKIK 1709
            RERSVPRYNEFRRRLFLIPIK WEDLTSD DAIE IRAIYGDDVEKLDLLVGL+AEKKIK
Sbjct: 481  RERSVPRYNEFRRRLFLIPIKSWEDLTSDKDAIETIRAIYGDDVEKLDLLVGLMAEKKIK 540

Query: 1710 GFAISETAFNIFILMASRRLEADRFLTSNFNEKTYTKKGMQWVKTTEGLRDVINRHYPEI 1889
            GFAISETAFNIFILMASRRLEADRF TSNFNE+TYTKKGMQWVKTTEGLRDVINRHYPEI
Sbjct: 541  GFAISETAFNIFILMASRRLEADRFFTSNFNEETYTKKGMQWVKTTEGLRDVINRHYPEI 600

Query: 1890 TTNWMKSSSAFSVWDADY 1943
            T  WMKSSSAFSVWDADY
Sbjct: 601  TAKWMKSSSAFSVWDADY 618

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>AK106731:Os01g0619800 |
          Length = 260

 Score = 31.6 bits (70), Expect = 2.6
 Identities = 41/164 (25%), Positives = 62/164 (37%), Gaps = 5/164 (3%)
 Frame = +3

Query: 33  SSESSAHLHLQLVFLLPEVAMGSGLFKPRVHPDLRDVFSKMSFGDKIGFLFIHAFDKRNL 212
           SS  S H HL+L++++P   M   L      P+       +   D +    +  +D    
Sbjct: 75  SSNLSTHHHLKLIYVVPAPRM-KELNNQEFRPE-----DLLQLADSVDGFSLMTYDFSGP 128

Query: 213 WHKMP-VPIGLLYLNTRRTLLEKYNLLAVGSSH----GAPFDPKEFPYRTGDGKYNDPHN 377
            +  P  P+  +  +  +TLL      + G SH    G  F   +F    GDG      N
Sbjct: 129 QNPGPSAPLSWIQYSL-KTLLAAKGSASHGHSHMIFLGINFYGNDFLLSKGDG-----GN 182

Query: 378 AEAGSQYTFFGRNMKPVDQQDELMSPDPFVVATKLLARREYKDT 509
           A  G  + +     KP  Q DE  S   FV + K L    +  T
Sbjct: 183 AITGRDFIYLLDKYKPSLQWDEKSSEHFFVYSDKGLRHAVFYPT 226

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>Os04g0622000:Os04g0622000 |
          Length = 338

 Score = 30.0 bits (66), Expect = 7.5
 Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 6/79 (7%)
 Frame = +3

Query: 81  PEVAMGSGLFKPRVHPDLRDVFSKMSFGDKIGFLFIHAFDKRNLWHKMPVPIGLLYLNTR 260
           P +A G G +K  +   +  +F +   G    +      DKR++W   PV +  L L+  
Sbjct: 93  PNLAAGLGEYKTILFSQINILFRRCGSGATRSWRRCGK-DKRSIWFNAPVEVDRLGLHDY 151

Query: 261 RTL------LEKYNLLAVG 299
            T+      LE+ NLL VG
Sbjct: 152 HTVIKWEITLEEKNLLRVG 170

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>AK102859:Os08g0528700 |
          Length = 187

 Score = 30.0 bits (66), Expect = 7.5
 Identities = 17/31 (54%), Positives = 19/31 (61%), Gaps = 2/31 (6%)
 Frame = -2

Query: 871 PLRTSPDNGTPF--SSCKRRPSSPITSFPSA 785
           P RTSP +  P   SS  RRP+SP  S PSA
Sbjct: 80  PARTSPRSSPPSHGSSAPRRPASPSASPPSA 110

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>AK066974:Os02g0600200 |
          Length = 266

 Score = 29.6 bits (65), Expect = 9.8
 Identities = 21/82 (25%), Positives = 38/82 (46%), Gaps = 1/82 (1%)
 Frame = +3

Query: 891  LQALFVKEHNAVCDAIKEEHPNLSDEELYRYA-KLVTSAVIAKIHTIDWTVELLKTKTLR 1067
            L A+  K+ + + D  KE++  L  +  +RY  K+V +  +    T    +EL K + L+
Sbjct: 140  LAAVIYKDEDDIIDTAKEQYRVLKTDNEFRYGYKVVENGNLRSALTTSNVIELPKKEELK 199

Query: 1068 AGMRANWYGLLGKKIKDTFGHI 1133
                      +  K+KD FG +
Sbjct: 200  T---------VVDKVKDFFGDV 212


  Database: orf_amino.fa.out
    Posted date:  Jul 26, 2007 11:37 AM
  Number of letters in database: 15,399,855
  Number of sequences in database:  40,041
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 40041
Number of Hits to DB: 70,272,253
Number of extensions: 1698844
Number of successful extensions: 4354
Number of sequences better than 10.0: 6
Number of HSP's gapped: 4350
Number of HSP's successfully gapped: 6
Length of query: 779
Length of database: 15,399,855
Length adjustment: 108
Effective length of query: 671
Effective length of database: 11,075,427
Effective search space: 7431611517
Effective search space used: 7431611517
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

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full-length cDNA and EST

Clone Information

BLAST Search Result