Clone Information

BLAST Search Result

Clone Name	FLbaf54n21
Clone Library Name	barley_pub

No.	Definition	Score (bits)	E Value
1	AK099091:Os07g0247000	32	0.45
2	AK072232:Os07g0247000	32	0.45
3	Os05g0188700:Os05g0188700	32	0.76
4	AK110523:Os07g0541600	31	1.3
5	AK119355:Os01g0390600	30	1.7
6	AK111148:Os11g0622200	30	2.9
7	Os09g0540900:Os09g0540900	29	3.8
8	Os05g0573900:Os05g0573900	29	3.8
9	AK119864:Os03g0626100	29	3.8
10	AK102856:Os02g0130900	29	3.8
11	AK069369:Os04g0323500	29	3.8
12	AK064188:Os12g0485300	29	3.8
13	AK061480:Os04g0323500	29	3.8
14	Os03g0277300:Os03g0277300	29	4.9
15	AK109026:	29	4.9
16	AK106029:	29	4.9
17	AK065227:Os01g0811300	29	4.9
18	Os07g0478700:Os07g0478700	28	6.4
19	Os03g0134000:Os03g0134000	28	6.4
20	AK110050:	28	6.4
21	AK111026:Os03g0192600	28	6.4
22	AK106673:Os09g0513800	28	6.4
23	Os02g0138000:Os02g0138000	28	8.4
24	Os01g0146700:Os01g0146700	28	8.4
25	AK110118:	28	8.4
26	AK109980:	28	8.4
27	AK072487:Os10g0564200	28	8.4

>AK099091:Os07g0247000 |
          Length = 361
 Score = 32.3 bits (72), Expect = 0.45
 Identities = 28/105 (26%), Positives = 41/105 (39%), Gaps = 1/105 (0%)
 Frame = +2

Query: 23  PFFSSSAAEGEQPGTLTLTALRSARRCDHGGFPPHGGDEDAP-SGDASRGGASPNLPHPA 199
           P  S S++    P     ++   +      G P  G     P SG +S G AS     P+
Sbjct: 244 PSSSPSSSPSSSPSGSPSSSPSGSPSGSPAGSPAGGPTAGGPASGPSSYGAASGPAEGPS 303

Query: 200 PAAFQINPCLGGGTPSASNPAEGPAVS*AFEIHHSLEGETPCRAS 334
            +     P  G     A+ PAEGP+       + S EG +P  +S
Sbjct: 304 SSGAASGPAEGPSPSGAAGPAEGPS------SYGSTEGPSPSPSS 342

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>AK072232:Os07g0247000 |
          Length = 361

 Score = 32.3 bits (72), Expect = 0.45
 Identities = 28/105 (26%), Positives = 41/105 (39%), Gaps = 1/105 (0%)
 Frame = +2

Query: 23  PFFSSSAAEGEQPGTLTLTALRSARRCDHGGFPPHGGDEDAP-SGDASRGGASPNLPHPA 199
           P  S S++    P     ++   +      G P  G     P SG +S G AS     P+
Sbjct: 244 PSSSPSSSPSSSPSGSPSSSPSGSPSGSPAGSPAGGPTAGGPASGPSSYGAASGPAEGPS 303

Query: 200 PAAFQINPCLGGGTPSASNPAEGPAVS*AFEIHHSLEGETPCRAS 334
            +     P  G     A+ PAEGP+       + S EG +P  +S
Sbjct: 304 SSGAASGPAEGPSPSGAAGPAEGPS------SYGSTEGPSPSPSS 342

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>Os05g0188700:Os05g0188700 |
          Length = 440

 Score = 31.6 bits (70), Expect = 0.76
 Identities = 19/47 (40%), Positives = 20/47 (42%)
 Frame = +2

Query: 62  GTLTLTALRSARRCDHGGFPPHGGDEDAPSGDASRGGASPNLPHPAP 202
           G + L A R  RR   GG   H      P GDA  GG  P  PH  P
Sbjct: 102 GGVLLGAARRRRRVGRGG-RRHRPQPRQPEGDAPGGGVPPRPPHVPP 147

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>AK110523:Os07g0541600 |
          Length = 129

 Score = 30.8 bits (68), Expect = 1.3
 Identities = 17/47 (36%), Positives = 23/47 (48%)
 Frame = +1

Query: 166 GRGFAESSAPSTSSFPNQPLPWRRNTVRL*SSRRTSCILSFRNPPFP 306
           G   A S+AP  +S    P PW R       +RR++ +L  R PP P
Sbjct: 29  GGSAARSTAPPVTSTTRGPAPWPR-------TRRSASLLRQRQPPSP 68

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>AK119355:Os01g0390600 |
          Length = 657

 Score = 30.4 bits (67), Expect = 1.7
 Identities = 23/69 (33%), Positives = 28/69 (40%), Gaps = 6/69 (8%)
 Frame = +2

Query: 29  FSSSAAEGEQPGTLTLTALRSARRCDHGGFPPHGGDEDAPSGDASRGG------ASPNLP 190
           F     E  QP  +TL  + +A  CDHG   P G    A  G A R G       + NL 
Sbjct: 130 FRDMLTEAVQPNEVTLAEVLTAMACDHGAPAPVG----AAHGVAVRRGLDGFVIVATNLV 185

Query: 191 HPAPAAFQI 217
           H   A  Q+
Sbjct: 186 HAYGAVSQV 194

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>AK111148:Os11g0622200 |
          Length = 181

 Score = 29.6 bits (65), Expect = 2.9
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +2

Query: 122 PHGGDEDAPSGDASRGGASPNLPHPAPAAFQINPCLGGGTPSASNP 259
           P GGD +   GD++     P L  P P A  +  CL   TP +  P
Sbjct: 49  PTGGDVEEAGGDSNFRRRQPKLNRPCPRASSLPTCL--PTPVSHQP 92

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>Os09g0540900:Os09g0540900 |
          Length = 574

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 29/102 (28%), Positives = 39/102 (38%), Gaps = 7/102 (6%)
 Frame = +2

Query: 50  GEQPGTLTLTALRSARRCDHGGFPPHGGDEDAPS-GDASRGGASPNL------PHPAPAA 208
           G  PG+    A  +A R   G +P     +   S  D S   AS +L      PHP    
Sbjct: 99  GGGPGSWPALAESAAAR---GSWPKSASSDSLKSLSDGSAPSASEDLIVPSVQPHPVA-- 153

Query: 209 FQINPCLGGGTPSASNPAEGPAVS*AFEIHHSLEGETPCRAS 334
              NP  GG  P++S P     V    E + + +   P R S
Sbjct: 154 ---NPISGGSNPTSSPPPPNATVVVTSEQNGNTDQSNPVRHS 192

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>Os05g0573900:Os05g0573900 |
          Length = 694

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 25/71 (35%), Positives = 30/71 (42%), Gaps = 5/71 (7%)
 Frame = +2

Query: 158 ASRGGASPNLPHPAP--AAFQINPCLGGGTPSASNPAEGPA---VS*AFEIHHSLEGETP 322
           +S G A P  P P P  AA + +      T S+S PA GP    V    E +HSL     
Sbjct: 332 SSSGSAGPPAPPPPPPPAAKRTSRTSTPATTSSSAPASGPCVRRVPEVVEFYHSLMRRDS 391

Query: 323 CRAS*AEEGGA 355
            R      GGA
Sbjct: 392 KRDGGGGGGGA 402

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>AK119864:Os03g0626100 |
          Length = 570

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 26/96 (27%), Positives = 32/96 (33%), Gaps = 4/96 (4%)
 Frame = +2

Query: 407 QESAQGSLYTSQASTRGPSVVQDN*NQEGD*FCF----VGGYSCYFCSHLLCLDCWS*NS 574
           ++S+      + A T  P  V    N      CF    +G  S   C H  C DCW    
Sbjct: 123 EKSSSSGSSMAMAKTNPPGSVAVTCN-----VCFEEYPLGSVSAMDCGHCFCNDCW---- 173

Query: 575 KV*WTSSYC**SAHSTRTWACCYL*GSAQARCGEVK 682
                          T  +A     GS Q RC EVK
Sbjct: 174 ---------------TEYFAAAVSDGSKQMRCMEVK 194

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>AK102856:Os02g0130900 |
          Length = 108

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 25/90 (27%), Positives = 33/90 (36%), Gaps = 2/90 (2%)
 Frame = +2

Query: 11  TERIPFFSSSAAEGEQPGTLTLTALRSARRCDHGGFPPHGGDEDAPSGDASRGGAS--PN 184
           T R    +SSA     P   T +A  SA        P       +PSG  SRG +S  P 
Sbjct: 2   TSRSYASTSSARSSPPPRPPTTSAPTSASTTRSSSSPSRSPGNQSPSGSPSRGCSSTPPP 61

Query: 185 LPHPAPAAFQINPCLGGGTPSASNPAEGPA 274
           LP  A             +PS +  +  P+
Sbjct: 62  LPSAATRGTMSRSASRSSSPSTTPSSPPPS 91

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>AK069369:Os04g0323500 |
          Length = 112

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 24/77 (31%), Positives = 28/77 (36%)
 Frame = +2

Query: 44  AEGEQPGTLTLTALRSARRCDHGGFPPHGGDEDAPSGDASRGGASPNLPHPAPAAFQINP 223
           A G++     LT+  S RR D  G     G      G   RG  +   P          P
Sbjct: 33  AGGDEEERRRLTSSLSLRRTDDDG---DDGAACGKPGQRKRGSDARRRPQRCRRQLLGRP 89

Query: 224 CLGGGTPSASNPAEGPA 274
             GGG PSA   A G A
Sbjct: 90  ADGGGEPSARGEAGGGA 106

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>AK064188:Os12g0485300 |
          Length = 101

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 17/60 (28%), Positives = 28/60 (46%)
 Frame = -2

Query: 793 IGARS*TSIYRCQTIDRHFKRHT*HGAGKELTSLLQPLHFSTSCLCTPLQVAAGPCPCRM 614
           + + S   I RC  ++ H +RH    + ++   L       + CLC  +Q AA  C CR+
Sbjct: 43  VASTSICKITRCTCLNHH-RRHIRAESVRQCHHLYWIRLLKSLCLCGHIQAAASGCRCRI 101

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>AK061480:Os04g0323500 |
          Length = 112

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 24/77 (31%), Positives = 28/77 (36%)
 Frame = +2

Query: 44  AEGEQPGTLTLTALRSARRCDHGGFPPHGGDEDAPSGDASRGGASPNLPHPAPAAFQINP 223
           A G++     LT+  S RR D  G     G      G   RG  +   P          P
Sbjct: 33  AGGDEEERRRLTSSLSLRRTDDDG---DDGAACGKPGQRKRGSDARRRPQRCRRQLLGRP 89

Query: 224 CLGGGTPSASNPAEGPA 274
             GGG PSA   A G A
Sbjct: 90  ADGGGEPSARGEAGGGA 106

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>Os03g0277300:Os03g0277300 |
          Length = 653

 Score = 28.9 bits (63), Expect = 4.9
 Identities = 13/23 (56%), Positives = 14/23 (60%)
 Frame = +2

Query: 110 GGFPPHGGDEDAPSGDASRGGAS 178
           G   P G DEDAP+G A  GG S
Sbjct: 621 GAGGPAGMDEDAPNGSAGTGGGS 643

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>AK109026: |
          Length = 328

 Score = 28.9 bits (63), Expect = 4.9
 Identities = 19/55 (34%), Positives = 20/55 (36%), Gaps = 1/55 (1%)
 Frame = +2

Query: 113 GFPPHGGDEDAPSGDASRGGASPNLPH-PAPAAFQINPCLGGGTPSASNPAEGPA 274
           G  P G  E AP G A  GG +   P   AP          GG P      E PA
Sbjct: 221 GGAPEGAPEGAPEGGAPEGGDAGGAPQGGAPEGGAPEGGDAGGAPGGGGGGEPPA 275

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>AK106029: |
          Length = 316

 Score = 28.9 bits (63), Expect = 4.9
 Identities = 22/69 (31%), Positives = 26/69 (37%), Gaps = 18/69 (26%)
 Frame = +2

Query: 122 PHGGDEDAPSGDASRGGASPNLPHPAPA-AFQINPCLGGG-----------------TPS 247
           P GG  +   G  + G  +   P PAPA A   N C GG                  TP+
Sbjct: 91  PAGGSNNCAGGGGNGGMGNGCQPAPAPAPAGGSNNCAGGAGNGGAGGNGGMGKGCGPTPA 150

Query: 248 ASNPAEGPA 274
           A  PA  PA
Sbjct: 151 APQPAPAPA 159

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>AK065227:Os01g0811300 |
          Length = 736

 Score = 28.9 bits (63), Expect = 4.9
 Identities = 25/79 (31%), Positives = 34/79 (43%), Gaps = 9/79 (11%)
 Frame = +2

Query: 62  GTLTLTALRS----ARRCDHGGFPPHGGDEDAPSGDASRGGASPNLPHPAPAAFQINPCL 229
           G+L + A+RS      +   GG    GGD+DA  G  + GG   +      AAF     +
Sbjct: 59  GSLNIPAVRSFAAFGGQDASGGKTAGGGDQDASGGKTAAGG-DQDAGRGETAAFGGQETV 117

Query: 230 GG-----GTPSASNPAEGP 271
            G     GTP+    A GP
Sbjct: 118 RGEFVANGTPNVGASATGP 136

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>Os07g0478700:Os07g0478700 |
          Length = 1489

 Score = 28.5 bits (62), Expect = 6.4
 Identities = 25/100 (25%), Positives = 35/100 (35%), Gaps = 13/100 (13%)
 Frame = +2

Query: 20   IPFFSSSAAEGEQPGTLTLTALRSARRCDHGGFPPHGG-------------DEDAPSGDA 160
            + F     A      T T     +AR    GG PP                   APS D+
Sbjct: 847  LSFLDDFTASSLPTPTSTRRTTAAARTSSPGGSPPASSATIDGHHCWHPLPGSPAPSADS 906

Query: 161  SRGGASPNLPHPAPAAFQINPCLGGGTPSASNPAEGPAVS 280
            S     P      PA+   +P    G+P+++ P+  P VS
Sbjct: 907  SAPSVDP------PASPAGSPTTTAGSPASTAPSPPPVVS 940

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>Os03g0134000:Os03g0134000 |
          Length = 1129

 Score = 28.5 bits (62), Expect = 6.4
 Identities = 15/33 (45%), Positives = 20/33 (60%)
 Frame = +2

Query: 662 ARCGEVKGLKKRCQFFSSTMLCMSLEMPVYCLA 760
           ARC E+ G KKRC  FSS +L + L +   C +
Sbjct: 860 ARC-ELTGTKKRCAVFSSQLLFIFLLLYANCFS 891

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>AK110050: |
          Length = 910

 Score = 28.5 bits (62), Expect = 6.4
 Identities = 15/45 (33%), Positives = 21/45 (46%)
 Frame = -2

Query: 292 FESSRYSWSFCWIRGGRCSSSKARVDLESCWCWVRKIRRSPSPGC 158
           + + R  W +   R G     ++ V L  CWCW   +RR PS  C
Sbjct: 2   YAALRVGWRWEVGRDGG-EDGRSEVRLMRCWCWA--VRRIPSAEC 43

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>AK111026:Os03g0192600 |
          Length = 82

 Score = 28.5 bits (62), Expect = 6.4
 Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 1/31 (3%)
 Frame = +2

Query: 179 PNLPH-PAPAAFQINPCLGGGTPSASNPAEG 268
           P++P  PAPA F   P  GG +PSAS    G
Sbjct: 8   PSIPLIPAPAPFSGAPFFGGSSPSASPAGTG 38

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>AK106673:Os09g0513800 |
          Length = 544

 Score = 28.5 bits (62), Expect = 6.4
 Identities = 9/20 (45%), Positives = 10/20 (50%)
 Frame = +2

Query: 503 CFVGGYSCYFCSHLLCLDCW 562
           C     SC  CSH  C +CW
Sbjct: 97  CAANAMSCAGCSHFYCHECW 116

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>Os02g0138000:Os02g0138000 |
          Length = 694

 Score = 28.1 bits (61), Expect = 8.4
 Identities = 16/54 (29%), Positives = 21/54 (38%), Gaps = 4/54 (7%)
 Frame = +2

Query: 119 PPHGGDEDAPSGDASRGGASPNLPHPAPAAFQI----NPCLGGGTPSASNPAEG 268
           PP G     PS  +  GG      HP+P         +P     TPS  +P +G
Sbjct: 459 PPGGSSSTTPSYPSPNGGKPSTPSHPSPPGSTTPSYPSPPSSSTTPSYHSPPQG 512

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>Os01g0146700:Os01g0146700 |
          Length = 1181

 Score = 28.1 bits (61), Expect = 8.4
 Identities = 19/63 (30%), Positives = 29/63 (46%), Gaps = 9/63 (14%)
 Frame = +2

Query: 119 PPHGGDEDAPSGDASRGGASPNLPHPAPA-------AFQINPCLG--GGTPSASNPAEGP 271
           PP      A +  A+RG   P+ P PAP+       A    P  G  GG+P+ +  ++  
Sbjct: 511 PPRAKFVHAGTSSAARGAVEPSTPPPAPSSIGPRSPATLAGPEAGPHGGSPAGAATSQPG 570

Query: 272 AVS 280
           A+S
Sbjct: 571 AIS 573

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>AK110118: |
          Length = 1243

 Score = 28.1 bits (61), Expect = 8.4
 Identities = 25/93 (26%), Positives = 41/93 (44%), Gaps = 6/93 (6%)
 Frame = +2

Query: 53   EQPGTLTLTALRSARRCDHGGFPPHGGDEDAPSGDASRGG--ASPNLPH----PAPAAFQ 214
            + P    +++L +    +        G    PSGD  RG   ++P + H    PAP    
Sbjct: 1047 DSPSNFDISSLMATSAMELEALFREAGLSAGPSGD-QRGWETSTPAMQHSALGPAPT--- 1102

Query: 215  INPCLGGGTPSASNPAEGPAVS*AFEIHHSLEG 313
            ++   GG  PSA +  +G A+S    +  S+EG
Sbjct: 1103 LHGAAGGREPSAQHYVQGEALSFYDSVGGSIEG 1135

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>AK109980: |
          Length = 1034

 Score = 28.1 bits (61), Expect = 8.4
 Identities = 25/86 (29%), Positives = 37/86 (43%), Gaps = 10/86 (11%)
 Frame = +2

Query: 47  EGEQPGTLTLTALR-SARRCDHGGFPPH------GGDEDAPSGDASRGGASPNLPHPAPA 205
           EG  P +++ ++ + SA+      F P+      GG    P+   S   A+ + P PA A
Sbjct: 645 EGGMPSSMSTSSFKLSAKASSFKPFNPNAATFTPGGASFTPAAPPSNAAAAAS-PAPAAA 703

Query: 206 A---FQINPCLGGGTPSASNPAEGPA 274
           A       P L    P+AS  A  PA
Sbjct: 704 AGPSLSARPSLTSTPPTASTSAAAPA 729

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>AK072487:Os10g0564200 |
          Length = 336

 Score = 28.1 bits (61), Expect = 8.4
 Identities = 28/106 (26%), Positives = 40/106 (37%)
 Frame = +2

Query: 155 DASRGGASPNLPHPAPAAFQINPCLGGGTPSASNPAEGPAVS*AFEIHHSLEGETPCRAS 334
           D + GGA    P+P        P     +PS ++P+   + S       S E E      
Sbjct: 142 DLAGGGAPDPSPNPIIKIKSKGPAAAAASPSPASPSPVSSPS-------SEEDE------ 188

Query: 335 *AEEGGAV*THCQRPRRFHGLLAGQESAQGSLYTSQASTRGPSVVQ 472
             EE  A      R R  HGL++   S  G  +T   ++R   V Q
Sbjct: 189 -EEEAAA---DAGRSRSLHGLISNGGSGSGLRFTIPKASRSKPVAQ 230


  Database: orf_amino.fa.out
    Posted date:  Jul 26, 2007 11:37 AM
  Number of letters in database: 15,399,855
  Number of sequences in database:  40,041
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 40041
Number of Hits to DB: 25,699,572
Number of extensions: 634805
Number of successful extensions: 2617
Number of sequences better than 10.0: 27
Number of HSP's gapped: 2588
Number of HSP's successfully gapped: 27
Length of query: 292
Length of database: 15,399,855
Length adjustment: 100
Effective length of query: 192
Effective length of database: 11,395,755
Effective search space: 2187984960
Effective search space used: 2187984960
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

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full-length cDNA and EST

Clone Information

BLAST Search Result