Clone Information

BLAST Search Result

Clone Name	FLbaf3h07
Clone Library Name	barley_pub

No.	Definition	Score (bits)	E Value
1	AY224436:Os12g0137500	578	e-165
2	AK070934:Os11g0141000	573	e-163
3	AK058901:Os11g0141000	498	e-141
4	Z54153:	315	6e-86
5	AK121815:Os10g0552400	30	3.5
6	AK100564:Os03g0395300	30	6.0
7	AK065704:Os02g0139000	30	6.0
8	AK069534:Os11g0661200	29	7.9

>AY224436:Os12g0137500 |
          Length = 418
 Score =  578 bits (1491), Expect = e-165
 Identities = 301/418 (72%), Positives = 330/418 (78%), Gaps = 4/418 (0%)
 Frame = +3

Query: 108  MAEVEDVSNRMEAQLRVHXXXXXXXXXXXXXXX----RXXXXXXXXXXXXXDQEGIRKGV 275
            M EVE+VSN+M+AQ+R+H                   +             DQEGIRKGV
Sbjct: 1    MVEVEEVSNKMQAQMRLHPAAAAEEEDADLPLPALFDKASHLHSLASSSSLDQEGIRKGV 60

Query: 276  DLLRRCDEMVSKLGLFSSNETKEDISTANLKYLLVPYYLGEMIEQVEQEDRIPVLKASQD 455
            DLLRRCDEMVSKLGLFSSNETK+D+STANLKYLLVPYYLGEM E+V QEDRIPVLKASQD
Sbjct: 61   DLLRRCDEMVSKLGLFSSNETKDDVSTANLKYLLVPYYLGEMTERVAQEDRIPVLKASQD 120

Query: 456  HLKEFISVCEALELIPEAELQLYRQGQSETGANRRAQKVARYKRQKAAEMKLQEIKQRKE 635
            HLKEFIS+CEALELI E EL+L RQ Q +T ANRRAQKVAR+KRQKAAE KL EIK+RKE
Sbjct: 121  HLKEFISICEALELISEDELELSRQKQPDTMANRRAQKVARFKRQKAAETKLLEIKERKE 180

Query: 636  RRGRSLRATALSAPIXXXXXXXXXXXXXXXXXAWLATISLSLCKAFDLVDMLKKEEEMLL 815
            RR RSLRA ALSAPI                 AWLATISL+LCKAFDL+DMLKKEEEMLL
Sbjct: 181  RRRRSLRAAALSAPIEAGEEDAFEDDGEEEREAWLATISLALCKAFDLLDMLKKEEEMLL 240

Query: 816  AVKERKEKDGNAFAREMLDERRQKAEEWHHNAASRAPYSKPAAPITCATFAQDVIEGRAS 995
            AVKER+ KDGNAFAREMLDER +KAE WHHNAA+RAPYSKPA PITCATFAQDVIEGRAS
Sbjct: 241  AVKERQAKDGNAFAREMLDERTKKAEAWHHNAANRAPYSKPADPITCATFAQDVIEGRAS 300

Query: 996  VSQAHDHKHQPLIFGPASLVGGGLTSERERMAAQVFQPSFRMPTMSIEQAGLAEMKMMEQ 1175
            VSQAH+HKHQPLIFGPASLVGGGLTSERERMAAQVFQPS+R+PTMSIE+AGL EMKMME+
Sbjct: 301  VSQAHEHKHQPLIFGPASLVGGGLTSERERMAAQVFQPSYRLPTMSIEEAGLREMKMMEK 360

Query: 1176 WQESTAKMIQEASSSWHKDDTSLXXXXXXXXXXXXXXXXXWKDENPRGAGNKKLTPCG 1349
            WQE TAKMIQE++S+WHKD +                   WKD+NPRGAGNKKLTPCG
Sbjct: 361  WQERTAKMIQESNSAWHKDGSRSAQEDEDAEEEKARAWDDWKDDNPRGAGNKKLTPCG 418

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>AK070934:Os11g0141000 |
          Length = 418

 Score =  573 bits (1477), Expect = e-163
 Identities = 298/418 (71%), Positives = 328/418 (78%), Gaps = 4/418 (0%)
 Frame = +3

Query: 108  MAEVEDVSNRMEAQLRVHXXXXXXXXXXXXXXX----RXXXXXXXXXXXXXDQEGIRKGV 275
            M EVE+VSN+M+ Q+R+H                   +             DQEGIRKGV
Sbjct: 1    MVEVEEVSNKMQVQMRLHPAAAAEEEDADLPLPALFDKASHLHSLASSSSLDQEGIRKGV 60

Query: 276  DLLRRCDEMVSKLGLFSSNETKEDISTANLKYLLVPYYLGEMIEQVEQEDRIPVLKASQD 455
            DLLRRCDEMVSK+GLFSSNETK+DISTANLKYLLVPYYLGEM E+V QEDRIPVLKASQD
Sbjct: 61   DLLRRCDEMVSKVGLFSSNETKDDISTANLKYLLVPYYLGEMTERVAQEDRIPVLKASQD 120

Query: 456  HLKEFISVCEALELIPEAELQLYRQGQSETGANRRAQKVARYKRQKAAEMKLQEIKQRKE 635
            HLKEFIS+CEALELI E EL++ RQ Q +T ANRRAQKVAR+KRQKAAE KL EIK+RKE
Sbjct: 121  HLKEFISICEALELISEDELEISRQKQPDTMANRRAQKVARFKRQKAAETKLLEIKERKE 180

Query: 636  RRGRSLRATALSAPIXXXXXXXXXXXXXXXXXAWLATISLSLCKAFDLVDMLKKEEEMLL 815
            RR RSLRA ALSAPI                 AWLATISL+LCKAFDL+DMLKKEEEML 
Sbjct: 181  RRRRSLRAAALSAPIEAGEEDAFEDDGEEEREAWLATISLALCKAFDLLDMLKKEEEMLP 240

Query: 816  AVKERKEKDGNAFAREMLDERRQKAEEWHHNAASRAPYSKPAAPITCATFAQDVIEGRAS 995
            AVKERK KDGNAFAREMLDER ++AE WHHNAA+RAPYSKPA PITCATFAQDVIEGRAS
Sbjct: 241  AVKERKAKDGNAFAREMLDERTKRAEAWHHNAANRAPYSKPADPITCATFAQDVIEGRAS 300

Query: 996  VSQAHDHKHQPLIFGPASLVGGGLTSERERMAAQVFQPSFRMPTMSIEQAGLAEMKMMEQ 1175
            VSQAH+HKHQPLIFGPASLVGGGLTSERERMAAQVFQPS+R+PTMSIE+AGL EMKMME+
Sbjct: 301  VSQAHEHKHQPLIFGPASLVGGGLTSERERMAAQVFQPSYRLPTMSIEEAGLREMKMMEK 360

Query: 1176 WQESTAKMIQEASSSWHKDDTSLXXXXXXXXXXXXXXXXXWKDENPRGAGNKKLTPCG 1349
            WQE TAKMIQE++S+WHKD +                   WKD+NPRGAGNKKLTPCG
Sbjct: 361  WQERTAKMIQESNSAWHKDGSRSAQEDEDAEEEKARGWDDWKDDNPRGAGNKKLTPCG 418

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>AK058901:Os11g0141000 |
          Length = 353

 Score =  498 bits (1282), Expect(2) = e-141
 Identities = 264/367 (71%), Positives = 283/367 (77%)
 Frame = +3

Query: 249  DQEGIRKGVDLLRRCDEMVSKLGLFSSNETKEDISTANLKYLLVPYYLGEMIEQVEQEDR 428
            DQEGIRKGVDLLRRCDEMVSK+GLFSSNETK+DISTANLKYLLVPYYLGEM E+V QEDR
Sbjct: 36   DQEGIRKGVDLLRRCDEMVSKVGLFSSNETKDDISTANLKYLLVPYYLGEMTERVAQEDR 95

Query: 429  IPVLKASQDHLKEFISVCEALELIPEAELQLYRQGQSETGANRRAQKVARYKRQKAAEMK 608
            IPVLKASQDHLKEFIS+CEALELI E EL++ RQ Q +T ANRRAQKVAR+KRQKAAE K
Sbjct: 96   IPVLKASQDHLKEFISICEALELISEDELEISRQKQPDTMANRRAQKVARFKRQKAAETK 155

Query: 609  LQEIKQRKERRGRSLRATALSAPIXXXXXXXXXXXXXXXXXAWLATISLSLCKAFDLVDM 788
            L EIK+RKERR RSLRA ALSAPI                 AWLATISL+LCKAFDL+DM
Sbjct: 156  LLEIKERKERRRRSLRAAALSAPIEAGEEDAFEDDGEEEREAWLATISLALCKAFDLLDM 215

Query: 789  LKKEEEMLLAVKERKEKDGNAFAREMLDERRQKAEEWHHNAASRAPYSKPAAPITCATFA 968
            LKKEEEML AVKERK KDGNAFAREMLDER ++AE WHHNAA+RAPYSKPA PITCATFA
Sbjct: 216  LKKEEEMLPAVKERKAKDGNAFAREMLDERTKRAEAWHHNAANRAPYSKPADPITCATFA 275

Query: 969  QDVIEGRASVSQAHDHKHQPLIFGPASLVGGGLTSERERMAAQVFQPSFRMPTMSIEQAG 1148
            QDVIEGRASVSQAH+HKHQPLIFGPASLVGGGLTSERERMAAQVFQPS+R+PTMSIE+A 
Sbjct: 276  QDVIEGRASVSQAHEHKHQPLIFGPASLVGGGLTSERERMAAQVFQPSYRLPTMSIEEA- 334

Query: 1149 LAEMKMMEQWQESTAKMIQEASSSWHKDDTSLXXXXXXXXXXXXXXXXXWKDENPRGAGN 1328
                                                             WKD+NPRGAGN
Sbjct: 335  ------------------------------------------------DWKDDNPRGAGN 346

Query: 1329 KKLTPCG 1349
            KKLTPCG
Sbjct: 347  KKLTPCG 353



 Score = 24.3 bits (51), Expect(2) = e-141
 Identities = 9/17 (52%), Positives = 11/17 (64%)
 Frame = +1

Query: 106 KWRRWRTSATGWKRSCA 156
           +W RWR SAT  +  CA
Sbjct: 1   EWSRWRKSATRCRCRCA 17

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>Z54153: |
          Length = 379

 Score =  315 bits (807), Expect = 6e-86
 Identities = 200/421 (47%), Positives = 242/421 (57%), Gaps = 7/421 (1%)
 Frame = +3

Query: 108  MAEVEDVSNRMEAQLRVHXXXXXXXXXXXXXXX----RXXXXXXXXXXXXXDQEGIRKGV 275
            M EVE+VSN+M+ Q+R+H                   +             DQEGIRKGV
Sbjct: 1    MVEVEEVSNKMQVQMRLHPAAAAEEEDADLPLPALFDKASHLHSLASSSSLDQEGIRKGV 60

Query: 276  DLLRRCDEMVSKLGLFSSNETKEDISTANLKYLLVPYYLGEMIEQVEQEDRIPVLKASQD 455
            DLLRRCDEMVSK+GLFSSNETK+DISTANLKYLLVPYYLGEM E+V QEDRIPVLKASQD
Sbjct: 61   DLLRRCDEMVSKVGLFSSNETKDDISTANLKYLLVPYYLGEMTERVAQEDRIPVLKASQD 120

Query: 456  HLKEFISVCEALELIPEAELQLYRQGQS---ETGANRRAQKVARYKRQKAAEMKLQEIKQ 626
            HLKEFIS+CEALELI E EL++ RQ      +   +RR    +  + QK +      IK+
Sbjct: 121  HLKEFISICEALELISEDELEISRQKNLIPWQIEEHRRLHGSSAKRLQKQS----SRIKE 176

Query: 627  RKERRGRSLRATALSAPIXXXXXXXXXXXXXXXXXAWLATISLSLCKAFDLVDMLKKEEE 806
            RKERR R   ++ LSAPI                   ++   +   + FDL+DMLKKEEE
Sbjct: 177  RKERRRRH-ESSCLSAPI--ELGGDLLRMMEKEREGMVSYYLIGSIEGFDLLDMLKKEEE 233

Query: 807  MLLAVKERKEKDGNAFAREMLDERRQKAEEWHHNAASRAPYSKPAAPITCATFAQDVIEG 986
            ++ AVKE K +        +L                          + C    Q  ++ 
Sbjct: 234  IVPAVKEGKRR----MVMHLL--------------------------VKCLMNVQKGLKH 263

Query: 987  RASVSQAHDHKHQPLIFGPASLVGGGLTSERERMAAQVFQPSFRMPTMSIEQAGLAEMKM 1166
               +     H    LI    SLV   LTSERERMAAQVFQPS+R+PTMSIE+AGL EMKM
Sbjct: 264  GTIMLPTVHHTPNQLI---QSLV--QLTSERERMAAQVFQPSYRLPTMSIEEAGLREMKM 318

Query: 1167 MEQWQESTAKMIQEASSSWHKDDTSLXXXXXXXXXXXXXXXXXWKDENPRGAGNKKLTPC 1346
            ME+WQE TAKMIQE++S+WHKD +                   WKD+NPRGAGNKKLTPC
Sbjct: 319  MEKWQERTAKMIQESNSAWHKDGSRSAQEDEDAEEEKARGWDDWKDDNPRGAGNKKLTPC 378

Query: 1347 G 1349
            G
Sbjct: 379  G 379

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>AK121815:Os10g0552400 |
          Length = 431

 Score = 30.4 bits (67), Expect = 3.5
 Identities = 25/84 (29%), Positives = 41/84 (48%), Gaps = 1/84 (1%)
 Frame = +3

Query: 414 EQEDRIPVLKASQDHLKEFISVCEALE-LIPEAELQLYRQGQSETGANRRAQKVARYKRQ 590
           EQ+D         D +KE    C+A + L+  A  + YR+ +++       QK   Y+  
Sbjct: 194 EQDDNNESELGFYDEIKE---ACKAADNLMMRALRESYRRQKADEEVVSSLQKAKEYE-- 248

Query: 591 KAAEMKLQEIKQRKERRGRSLRAT 662
              E+ L+E+K+RKE     LRA+
Sbjct: 249 ---ELYLEEVKKRKELEEALLRAS 269

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>AK100564:Os03g0395300 |
          Length = 776

 Score = 29.6 bits (65), Expect = 6.0
 Identities = 38/173 (21%), Positives = 71/173 (41%), Gaps = 23/173 (13%)
 Frame = +3

Query: 795  KEEEMLLAVKERKEKDGNAFAREMLDERRQKAEEWH---HNAASRAPYSKPAAPITCATF 965
            KEE  ++ +KE + ++  A   +ML +  Q+AE+     H+A      +K  A  T A  
Sbjct: 506  KEEIEMVYIKEAETREKMAELPKMLQQAAQEAEDAKVAPHSAQEELRKAKEEAEQTKAAA 565

Query: 966  AQDVIEGRASVSQAHDHKHQPLIFGPASLVGGGLTSERERMAAQVFQPSFRMPTMSIEQ- 1142
            A   I  RA + +    K    +    +LV      E E  ++   + S R  T+ I + 
Sbjct: 566  ATAEIRLRAVLKEIEASKASEKL----ALVAAQALQESEETSS--VEDSPRTITLPISEY 619

Query: 1143 -------------------AGLAEMKMMEQWQESTAKMIQEASSSWHKDDTSL 1244
                               A LA++++ ++ +  T + +Q+ +   HK   +L
Sbjct: 620  HSLSKRVYEAEELANERVAAALAQIELAKESETRTLERLQQETKEMHKKKDAL 672

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>AK065704:Os02g0139000 |
          Length = 467

 Score = 29.6 bits (65), Expect = 6.0
 Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 7/86 (8%)
 Frame = +3

Query: 366 KYLLVPYYLGEMIEQVEQEDRIPVLKASQDHLKE-FISVCEALELIPEAELQLYRQGQSE 542
           KY L  Y      ++ ++E +   +    DH K+    + EAL +  E + QL+ Q + +
Sbjct: 316 KYRLAKYLPETKEDKKQEEKKTKSVANGNDHAKKKSAQMAEALRMQMEVQKQLHEQLEVQ 375

Query: 543 TGANRRAQKVARY------KRQKAAE 602
                R ++ ARY      ++QKA E
Sbjct: 376 RQLQLRIEEHARYLQKILEEQQKARE 401

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>AK069534:Os11g0661200 |
          Length = 888

 Score = 29.3 bits (64), Expect = 7.9
 Identities = 14/36 (38%), Positives = 24/36 (66%)
 Frame = +3

Query: 534 QSETGANRRAQKVARYKRQKAAEMKLQEIKQRKERR 641
           QSE  ++ R +K  + + ++  EMKL E K+R++RR
Sbjct: 489 QSERTSSDREEKERQARLEQEQEMKLMEEKKREQRR 524


  Database: orf_amino.fa.out
    Posted date:  Jul 26, 2007 11:37 AM
  Number of letters in database: 15,399,855
  Number of sequences in database:  40,041
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 40041
Number of Hits to DB: 37,184,471
Number of extensions: 764120
Number of successful extensions: 2386
Number of sequences better than 10.0: 8
Number of HSP's gapped: 2368
Number of HSP's successfully gapped: 15
Length of query: 513
Length of database: 15,399,855
Length adjustment: 105
Effective length of query: 408
Effective length of database: 11,195,550
Effective search space: 4567784400
Effective search space used: 4567784400
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

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full-length cDNA and EST

Clone Information

BLAST Search Result