ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bast79e05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1OTUBL_ARATH (Q8LG98) Ubiquitin thioesterase otubain-like protein... 124 1e-28
2OTUB1_MOUSE (Q7TQI3) Ubiquitin thioesterase protein OTUB1 (EC 3.... 62 1e-09
3OTUB1_HUMAN (Q96FW1) Ubiquitin thioesterase protein OTUB1 (EC 3.... 62 1e-09
4OTUBL_DROME (Q9VL00) Ubiquitin thioesterase otubain-like protein... 60 3e-09
5OTUB2_MOUSE (Q9CQX0) Ubiquitin thioesterase protein OTUB2 (EC 3.... 51 2e-06
6OTUBL_CAEEL (Q9XVR6) Ubiquitin thioesterase otubain-like protein... 50 3e-06
7OTUB2_HUMAN (Q96DC9) Ubiquitin thioesterase protein OTUB2 (EC 3.... 50 3e-06
8Y483_CHLPN (Q9Z868) Hypothetical protein CPn_0483/CP0271/CPj0483... 32 1.2
9ALP16_SCHPO (P87244) Spindle pole body component alp16 (Altered ... 30 3.4
10NFL_XENLA (P35616) Neurofilament triplet L protein (Neurofilamen... 29 5.8
11SPCS2_MOUSE (Q9CYN2) Signal peptidase complex subunit 2 (EC 3.4.... 28 9.9

>OTUBL_ARATH (Q8LG98) Ubiquitin thioesterase otubain-like protein (EC 3.4.-.-)|
           (Ubiquitin-specific-processing protease otubain-like)
           (Deubiquitinating enzyme otubain-like)
          Length = 306

 Score =  124 bits (312), Expect = 1e-28
 Identities = 58/73 (79%), Positives = 68/73 (93%)
 Frame = +3

Query: 249 QVPYIGNKEPLSALAAEFQSGSPILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEH 428
           +VPY+G+KEPLS+LAAE+QSGSPIL EKIK+L  QY  +RRTRGDGNCF+RSFMFSYLEH
Sbjct: 45  KVPYVGDKEPLSSLAAEYQSGSPILLEKIKILDSQYIGIRRTRGDGNCFFRSFMFSYLEH 104

Query: 429 ILETQDRAEVERI 467
           ILE+QDRAEV+RI
Sbjct: 105 ILESQDRAEVDRI 117



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>OTUB1_MOUSE (Q7TQI3) Ubiquitin thioesterase protein OTUB1 (EC 3.4.-.-) (Otubain|
           1) (OTU domain-containing ubiquitin aldehyde-binding
           protein 1) (Ubiquitin-specific-processing protease
           OTUB1) (Deubiquitinating enzyme OTUB1)
          Length = 271

 Score = 61.6 bits (148), Expect = 1e-09
 Identities = 32/72 (44%), Positives = 46/72 (63%)
 Frame = +3

Query: 249 QVPYIGNKEPLSALAAEFQSGSPILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEH 428
           Q P +  +  LS L  E+     I Q+KIK L ++Y  +R+TR DGNCFYR+F FS+LE 
Sbjct: 44  QNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEA 103

Query: 429 ILETQDRAEVER 464
           +L+  D  E++R
Sbjct: 104 LLD--DSKELQR 113



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>OTUB1_HUMAN (Q96FW1) Ubiquitin thioesterase protein OTUB1 (EC 3.4.-.-) (Otubain|
           1) (OTU domain-containing ubiquitin aldehyde-binding
           protein 1) (Ubiquitin-specific-processing protease
           OTUB1) (Deubiquitinating enzyme OTUB1)
          Length = 271

 Score = 61.6 bits (148), Expect = 1e-09
 Identities = 32/72 (44%), Positives = 46/72 (63%)
 Frame = +3

Query: 249 QVPYIGNKEPLSALAAEFQSGSPILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEH 428
           Q P +  +  LS L  E+     I Q+KIK L ++Y  +R+TR DGNCFYR+F FS+LE 
Sbjct: 44  QNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEA 103

Query: 429 ILETQDRAEVER 464
           +L+  D  E++R
Sbjct: 104 LLD--DSKELQR 113



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>OTUBL_DROME (Q9VL00) Ubiquitin thioesterase otubain-like protein (EC 3.4.-.-)|
           (Ubiquitin-specific-processing protease otubain-like)
           (Deubiquitinating enzyme otubain-like)
          Length = 262

 Score = 60.1 bits (144), Expect = 3e-09
 Identities = 28/60 (46%), Positives = 42/60 (70%)
 Frame = +3

Query: 255 PYIGNKEPLSALAAEFQSGSPILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEHIL 434
           P +  + PL+ L AE+ SG  I   KI+ L ++Y  +RRTR DGNCF+R+F +SYLE+++
Sbjct: 31  PLVSEQLPLTCLYAEY-SGDEIFTAKIQDLSKKYKFIRRTRPDGNCFFRAFAYSYLEYLI 89



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>OTUB2_MOUSE (Q9CQX0) Ubiquitin thioesterase protein OTUB2 (EC 3.4.-.-) (Otubain|
           2) (OTU domain-containing ubiquitin aldehyde-binding
           protein 2) (Ubiquitin-specific-processing protease
           OTUB2) (Deubiquitinating enzyme OTUB2)
          Length = 234

 Score = 50.8 bits (120), Expect = 2e-06
 Identities = 25/57 (43%), Positives = 39/57 (68%), Gaps = 2/57 (3%)
 Frame = +3

Query: 318 ILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEHIL--ETQDRAEVERILKTLN 482
           I Q KI+ L +++ ++R+T+GDGNCFYR+  +SYLE +L    +     ER+L+T N
Sbjct: 27  IYQRKIQELSKRFTSIRKTKGDGNCFYRALGYSYLESLLGKSREILKFKERVLQTPN 83



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>OTUBL_CAEEL (Q9XVR6) Ubiquitin thioesterase otubain-like protein (EC 3.4.-.-)|
           (Ubiquitin-specific-processing protease otubain-like)
           (Deubiquitinating enzyme otubain-like)
          Length = 284

 Score = 50.1 bits (118), Expect = 3e-06
 Identities = 28/74 (37%), Positives = 40/74 (54%), Gaps = 1/74 (1%)
 Frame = +3

Query: 252 VPYIGNKEPLSALAAEFQSG-SPILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEH 428
           VP +    P S L AE+ +  S     K   L E Y  +R  RGDGNCFYR+ +   +E 
Sbjct: 41  VPLVATLAPFSILCAEYDNETSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEI 100

Query: 429 ILETQDRAEVERIL 470
           +L  +DRA +E+ +
Sbjct: 101 ML--KDRARLEKFI 112



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>OTUB2_HUMAN (Q96DC9) Ubiquitin thioesterase protein OTUB2 (EC 3.4.-.-) (Otubain|
           2) (OTU domain-containing ubiquitin aldehyde-binding
           protein 2) (Ubiquitin-specific-processing protease
           OTUB2) (Deubiquitinating enzyme OTUB2)
          Length = 234

 Score = 50.1 bits (118), Expect = 3e-06
 Identities = 25/57 (43%), Positives = 39/57 (68%), Gaps = 2/57 (3%)
 Frame = +3

Query: 318 ILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEHIL--ETQDRAEVERILKTLN 482
           I + KI+ L +++ A+R+T+GDGNCFYR+  +SYLE +L    +     ER+L+T N
Sbjct: 27  IYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPN 83



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>Y483_CHLPN (Q9Z868) Hypothetical protein CPn_0483/CP0271/CPj0483/CpB0503|
          Length = 1043

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = +3

Query: 378 GDGNCFYRSFMFSYLEHILETQDRAEV 458
           GDGNCFYR++   +L  + E   R ++
Sbjct: 262 GDGNCFYRAYAVGWLSALYEESSRNDI 288



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>ALP16_SCHPO (P87244) Spindle pole body component alp16 (Altered polarity|
           protein 16)
          Length = 759

 Score = 30.0 bits (66), Expect = 3.4
 Identities = 22/64 (34%), Positives = 32/64 (50%)
 Frame = +3

Query: 282 SALAAEFQSGSPILQEKIKLLGEQYDALRRTRGDGNCFYRSFMFSYLEHILETQDRAEVE 461
           SA  +EF+   P LQ + +++G    ALR    D +  Y S  FSY+ H+ + Q   E  
Sbjct: 606 SASVSEFEDVYPDLQFQCQVIG----ALRILFTDNSLNYYSKTFSYVLHLFQAQSDFESS 661

Query: 462 RILK 473
             LK
Sbjct: 662 VELK 665



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>NFL_XENLA (P35616) Neurofilament triplet L protein (Neurofilament light|
           polypeptide) (NF-L)
          Length = 543

 Score = 29.3 bits (64), Expect = 5.8
 Identities = 16/44 (36%), Positives = 22/44 (50%)
 Frame = -3

Query: 382 SPRVRLKASYCSPSNFIFSCKMGLPDWNSAAKAERGSLLPMYGT 251
           SPRV +++SY SPS   +S  +      S A +   S LP   T
Sbjct: 20  SPRVHIRSSYVSPSRTTYSPLVSTTMRRSYATSSSSSFLPSVDT 63



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>SPCS2_MOUSE (Q9CYN2) Signal peptidase complex subunit 2 (EC 3.4.-.-)|
           (Microsomal signal peptidase 25 kDa subunit) (SPase 25
           kDa subunit)
          Length = 226

 Score = 28.5 bits (62), Expect = 9.9
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = +1

Query: 217 GGGSDGAGPDPRCRTSVTRN 276
           GGGS GAG  P C TS +R+
Sbjct: 14  GGGSSGAGGGPSCGTSSSRS 33


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,358,797
Number of Sequences: 219361
Number of extensions: 709116
Number of successful extensions: 2145
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 2085
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2145
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3362826254
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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