ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bast74f02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SC5D_TOBAC (Q9ZT29) Delta-7-sterol-C5(6)-desaturase (EC 1.3.3.-)... 168 6e-42
2SC5D1_ARATH (Q39208) Delta-7-sterol-C5(6)-desaturase 1 (EC 1.3.3... 154 1e-37
3SC5D2_ARATH (Q9M883) Putative delta-7-sterol-C5(6)-desaturase 2 ... 135 4e-32
4SGLT_VIBPA (P96169) Sodium/glucose cotransporter (Na(+)/glucose ... 33 0.50
5BSU1_SCHPO (P33532) Vitamin B6 transporter bsu1 (Vitamin B6 upta... 31 1.9
6WBP11_BRARE (Q6P0D5) WW domain-binding protein 11 (WBP-11) 30 4.2
7NUOH_MYCTU (P65561) NADH-quinone oxidoreductase chain H (EC 1.6.... 30 4.2
8NUOH_MYCBO (P65562) NADH-quinone oxidoreductase chain H (EC 1.6.... 30 4.2
9PRP28_CRYNE (Q5KNF8) Pre-mRNA splicing ATP-dependent RNA helicas... 29 5.5
10GLB3_TYLHE (P13578) Extracellular globin IIB (Erythrocruorin) 29 7.2
11ATG13_EMENI (Q5BBK4) Autophagy-related protein 13 29 7.2
12RPGF3_RAT (Q9Z1C8) Rap guanine nucleotide exchange factor 3 (cAM... 28 9.4
13RPGF3_MOUSE (Q8VCC8) Rap guanine nucleotide exchange factor 3 (c... 28 9.4

>SC5D_TOBAC (Q9ZT29) Delta-7-sterol-C5(6)-desaturase (EC 1.3.3.-) (Delta-7-C-5|
           sterol desaturase) (Delta7-sterol-C5-desaturase)
          Length = 271

 Score =  168 bits (426), Expect = 6e-42
 Identities = 78/126 (61%), Positives = 93/126 (73%)
 Frame = +2

Query: 95  EDYWGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCF 274
           EDY   F EET +YN + L   +P    WW  LPH L+ WLRN IGG LLYF +GFLWCF
Sbjct: 2   EDYLKQFVEETSFYNRLVLGTFMPES--WWGPLPHMLQGWLRNYIGGVLLYFISGFLWCF 59

Query: 275 VIYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCFFHISE 454
            IY+ KRN YIPKDA+P+ +AM  QI VA KAMP+YCALPS+SE+MIE+GWT+CF  IS+
Sbjct: 60  YIYHLKRNVYIPKDAIPSNKAMLLQISVAMKAMPWYCALPSLSEYMIENGWTKCFARISD 119

Query: 455 VGWPMY 472
           VGW  Y
Sbjct: 120 VGWLSY 125



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>SC5D1_ARATH (Q39208) Delta-7-sterol-C5(6)-desaturase 1 (EC 1.3.3.-)|
           (Delta-7-C-5 sterol desaturase 1)
           (Delta7-sterol-C5-desaturase 1) (STEROL1 protein) (Dwarf
           7 protein)
          Length = 281

 Score =  154 bits (388), Expect = 1e-37
 Identities = 70/121 (57%), Positives = 88/121 (72%)
 Frame = +2

Query: 113 FREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFVIYYWK 292
           F +ET +YN I LS ++P     W  LPH L++WLRN + G LLYF +GFLWCF IYY K
Sbjct: 11  FVDETSFYNRIVLSHLLPAN--LWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYYLK 68

Query: 293 RNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCFFHISEVGWPMY 472
            N Y+PKDA+PT++AM+ Q+ VA KAMP+Y  LP+VSE MIE GWT+CF  I E GW +Y
Sbjct: 69  INVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASIGEFGWILY 128

Query: 473 F 475
           F
Sbjct: 129 F 129



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>SC5D2_ARATH (Q9M883) Putative delta-7-sterol-C5(6)-desaturase 2 (EC 1.3.3.-)|
           (Delta-7-C-5 sterol desaturase 2)
           (Delta7-sterol-C5-desaturase 2) (Homolog of DWF7
           protein)
          Length = 279

 Score =  135 bits (341), Expect = 4e-32
 Identities = 63/126 (50%), Positives = 84/126 (66%)
 Frame = +2

Query: 98  DYWGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV 277
           DY      ET +YN + LS ++P     W  LPH L++WLRN + G +LYF +GFLWCF 
Sbjct: 7   DYNDQIVNETSFYNRMVLSHLLPVN--LWEPLPHFLQTWLRNYLAGNILYFISGFLWCFY 64

Query: 278 IYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCFFHISEV 457
           IYY K N Y+PK+++PT +AM  QI VA KAMP+Y  LP+VSE+MIE GWT+C+  +   
Sbjct: 65  IYYLKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHF 124

Query: 458 GWPMYF 475
            W + F
Sbjct: 125 NWFLCF 130



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>SGLT_VIBPA (P96169) Sodium/glucose cotransporter (Na(+)/glucose symporter)|
          Length = 543

 Score = 32.7 bits (73), Expect = 0.50
 Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 5/60 (8%)
 Frame = +2

Query: 257 GFLWCFVIYYWKRNAYIPKD--AVPTVEAMKKQIIVAS---KAMPFYCALPSVSEHMIES 421
           G LW   +YYW  N YI +   A  +V   +K I+ A+     +PF   LP ++ ++I S
Sbjct: 254 GGLWVANLYYWGFNQYIIQRTLAAKSVSEAQKGIVFAAFLKLIVPFLVVLPGIAAYVITS 313



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>BSU1_SCHPO (P33532) Vitamin B6 transporter bsu1 (Vitamin B6 uptake protein 1)|
           (Changed amiloride resistance protein 1) (Amiloride
           transporter car1)
          Length = 526

 Score = 30.8 bits (68), Expect = 1.9
 Identities = 14/60 (23%), Positives = 28/60 (46%)
 Frame = +2

Query: 107 GLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFVIYY 286
           G+ R E  + + +F++ + P G   +    HP   W+   +G  ++  A G  W  ++ Y
Sbjct: 379 GVARPEARFTSALFITLLFPAGMFLFAFTCHPPFPWMSPIVGNSMVTVANGHNWMCILNY 438



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>WBP11_BRARE (Q6P0D5) WW domain-binding protein 11 (WBP-11)|
          Length = 640

 Score = 29.6 bits (65), Expect = 4.2
 Identities = 13/21 (61%), Positives = 14/21 (66%)
 Frame = -1

Query: 216 SQERSGCGSARHQPPPPGTTA 154
           S E +  GSA   PPPPGTTA
Sbjct: 512 SNEGAPSGSALQMPPPPGTTA 532



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>NUOH_MYCTU (P65561) NADH-quinone oxidoreductase chain H (EC 1.6.99.5) (NADH|
           dehydrogenase I, chain H) (NDH-1, chain H)
          Length = 410

 Score = 29.6 bits (65), Expect = 4.2
 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +2

Query: 176 GWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV-IYYWKRNAYIPK 313
           G W A P PL  W     G + L + T  +W F+ IY+W R A +P+
Sbjct: 265 GGWHA-PWPLNMWASANTGWWPLIWFTAKVWGFLFIYFWLR-ATLPR 309



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>NUOH_MYCBO (P65562) NADH-quinone oxidoreductase chain H (EC 1.6.99.5) (NADH|
           dehydrogenase I, chain H) (NDH-1, chain H)
          Length = 410

 Score = 29.6 bits (65), Expect = 4.2
 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +2

Query: 176 GWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV-IYYWKRNAYIPK 313
           G W A P PL  W     G + L + T  +W F+ IY+W R A +P+
Sbjct: 265 GGWHA-PWPLNMWASANTGWWPLIWFTAKVWGFLFIYFWLR-ATLPR 309



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>PRP28_CRYNE (Q5KNF8) Pre-mRNA splicing ATP-dependent RNA helicase PRP28 (EC|
           3.6.1.-)
          Length = 738

 Score = 29.3 bits (64), Expect = 5.5
 Identities = 16/47 (34%), Positives = 22/47 (46%)
 Frame = +2

Query: 104 WGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLL 244
           W +FRE+         S    GGG     +PHPLR+W  + I   +L
Sbjct: 294 WRIFRED--------FSIAARGGG-----IPHPLRNWRESAIPSQIL 327



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>GLB3_TYLHE (P13578) Extracellular globin IIB (Erythrocruorin)|
          Length = 148

 Score = 28.9 bits (63), Expect = 7.2
 Identities = 11/23 (47%), Positives = 13/23 (56%)
 Frame = +2

Query: 389 LPSVSEHMIESGWTRCFFHISEV 457
           LP VS H     W RCF  I++V
Sbjct: 120 LPQVSSHFNSGAWDRCFTRIADV 142



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>ATG13_EMENI (Q5BBK4) Autophagy-related protein 13|
          Length = 974

 Score = 28.9 bits (63), Expect = 7.2
 Identities = 13/39 (33%), Positives = 21/39 (53%)
 Frame = -1

Query: 207 RSGCGSARHQPPPPGTTALRKISLYQSVSSRNRPQ*SSP 91
           R+    +RH PPP  TT+ RK   +   ++ + P  +SP
Sbjct: 470 RTSLSDSRHMPPPSVTTSSRKPPSFGPDNANSSPNSASP 508



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>RPGF3_RAT (Q9Z1C8) Rap guanine nucleotide exchange factor 3 (cAMP-regulated|
           guanine nucleotide exchange factor I) (cAMP-GEFI)
           (Exchange factor directly activated by cAMP 1) (Epac 1)
          Length = 884

 Score = 28.5 bits (62), Expect = 9.4
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +1

Query: 295 QRLHPQRCCPYSRSYEEANNCCIKG 369
           +R+H  RCC Y   +E     CI+G
Sbjct: 4   KRMHRPRCCSYQLVFEHRRPSCIQG 28



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>RPGF3_MOUSE (Q8VCC8) Rap guanine nucleotide exchange factor 3 (cAMP-regulated|
           guanine nucleotide exchange factor I) (cAMP-GEFI)
           (Exchange factor directly activated by cAMP 1) (Epac 1)
          Length = 876

 Score = 28.5 bits (62), Expect = 9.4
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +1

Query: 295 QRLHPQRCCPYSRSYEEANNCCIKG 369
           +R+H  RCC Y   +E     CI+G
Sbjct: 4   KRMHRPRCCSYQLVFEHRRPSCIQG 28


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,117,491
Number of Sequences: 219361
Number of extensions: 843034
Number of successful extensions: 2659
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 2584
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2654
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3188886965
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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