ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | bast64g01 |
|---|---|
| Clone Library Name | barley_pub |
>MEGL_PSEPU (P13254) Methionine gamma-lyase (EC 4.4.1.11) (L-methioninase)| Length = 398 Score = 42.0 bits (97), Expect = 7e-04 Identities = 32/88 (36%), Positives = 42/88 (47%), Gaps = 3/88 (3%) Frame = +2 Query: 203 PAAALAAARHEFG--EHGGVNMS-IEASATFTVMEPDTMRRLFTGELGPERGDLYIYSRH 373 P A A H + +HGG + + +ATFT + F GE + YSR Sbjct: 8 PGFATRAIHHGYDPQDHGGALVPPVYQTATFTFPTVEYGAACFAGEQAG-----HFYSRI 62 Query: 374 FNPTVLALGRQMAALEGTEAAYCTASGM 457 NPT+ L +MA+LEG EA ASGM Sbjct: 63 SNPTLNLLEARMASLEGGEAGLALASGM 90
>METZ_PSEAE (P55218) O-succinylhomoserine sulfhydrylase (EC 2.5.1.-) (OSH| sulfhydrylase) Length = 403 Score = 40.8 bits (94), Expect = 0.002 Identities = 29/73 (39%), Positives = 36/73 (49%) Frame = +2 Query: 239 GEHGGVNMSIEASATFTVMEPDTMRRLFTGELGPERGDLYIYSRHFNPTVLALGRQMAAL 418 GEHG + +S F R F GE+ +YSR+ NPTV ++AAL Sbjct: 34 GEHGEA-LFTTSSYVFRTAADAAAR--FAGEVPGN-----VYSRYTNPTVRTFEERIAAL 85 Query: 419 EGTEAAYCTASGM 457 EG E A TASGM Sbjct: 86 EGAEQAVATASGM 98
>MET17_KLULA (Q92441) Protein MET17 [Includes: O-acetylhomoserine sulfhydrylase| (EC 2.5.1.49) (OAH sulfhydrylase) (Homocysteine synthase); O-acetylserine sulfhydrylase (EC 2.5.1.47) (OAS sulfhydrylase)] Length = 443 Score = 38.1 bits (87), Expect = 0.011 Identities = 19/33 (57%), Positives = 24/33 (72%) Frame = +2 Query: 356 YIYSRHFNPTVLALGRQMAALEGTEAAYCTASG 454 YIYSR NPT+ L +++AALEG AA T+SG Sbjct: 52 YIYSRIMNPTLDVLEKRLAALEGGIAALATSSG 84
>METB_HELPJ (Q9ZMW7) Cystathionine gamma-synthase (EC 2.5.1.48) (CGS)| (O-succinylhomoserine (thiol)-lyase) Length = 380 Score = 35.0 bits (79), Expect = 0.092 Identities = 25/72 (34%), Positives = 35/72 (48%), Gaps = 1/72 (1%) Frame = +2 Query: 245 HGGVNMSIEASA-TFTVMEPDTMRRLFTGELGPERGDLYIYSRHFNPTVLALGRQMAALE 421 HGG+N A + + + T R+ +G +G Y YSR NPT AL +A LE Sbjct: 9 HGGINEDATTGAVSVPIYQTSTYRQ---DAIGRHKG--YEYSRSGNPTRFALEELIADLE 63 Query: 422 GTEAAYCTASGM 457 G + ASG+ Sbjct: 64 GGVKGFAFASGL 75
>MET17_YEAST (P06106) Protein MET17 [Includes: O-acetylhomoserine sulfhydrylase| (EC 2.5.1.49) (OAH sulfhydrylase) (Homocysteine synthase); O-acetylserine sulfhydrylase (EC 2.5.1.47) (OAS sulfhydrylase)] Length = 443 Score = 34.3 bits (77), Expect = 0.16 Identities = 17/33 (51%), Positives = 21/33 (63%) Frame = +2 Query: 356 YIYSRHFNPTVLALGRQMAALEGTEAAYCTASG 454 Y+YSR NPT L ++AALEG AA +SG Sbjct: 53 YVYSRFQNPTSNVLEERIAALEGGAAALAVSSG 85
>CYSD_SCHPO (O13326) O-acetylhomoserine (thiol)-lyase (EC 2.5.1.49)| (O-acetylhomoserine sulfhydrylase) (OAH sulfhydrylase) (Homocysteine synthase) Length = 429 Score = 33.9 bits (76), Expect = 0.20 Identities = 17/33 (51%), Positives = 21/33 (63%) Frame = +2 Query: 356 YIYSRHFNPTVLALGRQMAALEGTEAAYCTASG 454 YIYSR NPT +++AALE AA T+SG Sbjct: 55 YIYSRMMNPTADVFEKRIAALEHGAAAIATSSG 87
>METB_ARATH (P55217) Cystathionine gamma-synthase, chloroplast precursor (EC| 2.5.1.48) (CGS) (O-succinylhomoserine (thiol)-lyase) Length = 563 Score = 33.5 bits (75), Expect = 0.27 Identities = 16/39 (41%), Positives = 24/39 (61%) Frame = +2 Query: 341 ERGDLYIYSRHFNPTVLALGRQMAALEGTEAAYCTASGM 457 +R + Y R+ NPT + L +++ALEG E+ ASGM Sbjct: 219 KRSVSFEYGRYGNPTTVVLEDKISALEGAESTLVMASGM 257
>CYSD_EMENI (P50125) O-acetylhomoserine (thiol)-lyase (EC 2.5.1.49)| (O-acetylhomoserine sulfhydrylase) (OAH sulfhydrylase) (Homocysteine synthase) Length = 437 Score = 33.1 bits (74), Expect = 0.35 Identities = 17/32 (53%), Positives = 21/32 (65%) Frame = +2 Query: 359 IYSRHFNPTVLALGRQMAALEGTEAAYCTASG 454 IYSR NPTV +++AALEG AA +SG Sbjct: 57 IYSRIMNPTVDVFEKRIAALEGGVAAVAASSG 88
>METB_HELPY (P56069) Cystathionine gamma-synthase (EC 2.5.1.48) (CGS)| (O-succinylhomoserine (thiol)-lyase) Length = 380 Score = 33.1 bits (74), Expect = 0.35 Identities = 24/72 (33%), Positives = 35/72 (48%), Gaps = 1/72 (1%) Frame = +2 Query: 245 HGGVNMSIEASA-TFTVMEPDTMRRLFTGELGPERGDLYIYSRHFNPTVLALGRQMAALE 421 HGG++ A + + + T R+ +G +G Y YSR NPT AL +A LE Sbjct: 9 HGGISEDATTGAVSVPIYQTSTYRQ---DAIGRHKG--YEYSRSGNPTRFALEELIADLE 63 Query: 422 GTEAAYCTASGM 457 G + ASG+ Sbjct: 64 GGVKGFAFASGL 75
>METC_ARATH (P53780) Cystathionine beta-lyase, chloroplast precursor (EC| 4.4.1.8) (CBL) (Beta-cystathionase) (Cysteine lyase) Length = 464 Score = 32.0 bits (71), Expect = 0.78 Identities = 27/102 (26%), Positives = 45/102 (44%) Frame = +2 Query: 152 NGSTEEKALKARRRESDPAAALAAARHEFGEHGGVNMSIEASATFTVMEPDTMRRLFTGE 331 N +T+ + E+ + L ++F ++ + +ATF +P + E Sbjct: 70 NNTTDSLNTMNIKEEASVSTLLVNLDNKFDPFDAMSTPLYQTATFK--QPSAI------E 121 Query: 332 LGPERGDLYIYSRHFNPTVLALGRQMAALEGTEAAYCTASGM 457 GP Y Y+R NPT AL +A L+ + A+C SGM Sbjct: 122 NGP-----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGM 158
>METB_ECOLI (P00935) Cystathionine gamma-synthase (EC 2.5.1.48) (CGS)| (O-succinylhomoserine (thiol)-lyase) Length = 386 Score = 30.4 bits (67), Expect = 2.3 Identities = 26/82 (31%), Positives = 37/82 (45%) Frame = +2 Query: 212 ALAAARHEFGEHGGVNMSIEASATFTVMEPDTMRRLFTGELGPERGDLYIYSRHFNPTVL 391 A+ + ++ ++G V I S+T+ FTG P D YSR NPT Sbjct: 9 AVRSGLNDDEQYGCVVPPIHLSSTYN----------FTGFNEPRAHD---YSRRGNPTRD 55 Query: 392 ALGRQMAALEGTEAAYCTASGM 457 + R +A LEG A T +GM Sbjct: 56 VVQRALAELEGGAGAVLTNTGM 77
>ATR_KLULA (Q6CT34) Serine/threonine-protein kinase MEC1 (EC 2.7.11.1)| (DNA-damage checkpoint kinase MEC1) (Mitosis entry checkpoint protein 1) (ATR homolog) Length = 2287 Score = 30.0 bits (66), Expect = 2.9 Identities = 29/107 (27%), Positives = 41/107 (38%), Gaps = 2/107 (1%) Frame = +2 Query: 143 GDDNGSTEEKALKARRRESDPAAALAAARHEFGEHGGVNMSIEASATFTVMEPDTMRRLF 322 G+D EEK LK R S A+A +H F SIE T + R+ + Sbjct: 661 GNDKSLVEEKKLKLLDRNSRQIFAVALVKHGF-------FSIETIQTLFLNTAPKFRKNY 713 Query: 323 TGELGPERGDLYIYSRHFNPTVLALGRQMAALEG--TEAAYCTASGM 457 LY+ VL + +Q+ L+G TE C S + Sbjct: 714 I--------TLYLPDYKTLAEVLKMSKQILTLDGQITETERCVLSSL 752
>UL56_HHV11 (P10240) Protein UL56| Length = 197 Score = 30.0 bits (66), Expect = 2.9 Identities = 21/70 (30%), Positives = 30/70 (42%) Frame = +2 Query: 119 PTPQNDGFGDDNGSTEEKALKARRRESDPAAALAAARHEFGEHGGVNMSIEASATFTVME 298 PT +G + G + + A +A RR + A A R FG G + + T V Sbjct: 64 PTSSGEGEASERGRSRQAAQRAARR-ARRRAERRAQRRSFGPGGLLATPLFLPETRLVAP 122 Query: 299 PDTMRRLFTG 328 PD R L +G Sbjct: 123 PDITRDLLSG 132
>FTHS_STAAW (Q8NW37) Formate--tetrahydrofolate ligase (EC 6.3.4.3)| (Formyltetrahydrofolate synthetase) (FHS) (FTHFS) Length = 555 Score = 29.6 bits (65), Expect = 3.8 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = +2 Query: 137 GFGDDNGSTEEKALKARRRESDPAAALAAAR-HEFGEHGGV 256 GFG D G+ + +KAR DPAA + A HGGV Sbjct: 300 GFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRALKMHGGV 340
>FTHS_STAAS (Q6G8J5) Formate--tetrahydrofolate ligase (EC 6.3.4.3)| (Formyltetrahydrofolate synthetase) (FHS) (FTHFS) Length = 555 Score = 29.6 bits (65), Expect = 3.8 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = +2 Query: 137 GFGDDNGSTEEKALKARRRESDPAAALAAAR-HEFGEHGGV 256 GFG D G+ + +KAR DPAA + A HGGV Sbjct: 300 GFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRALKMHGGV 340
>FTHS_STAAR (Q6GFX6) Formate--tetrahydrofolate ligase (EC 6.3.4.3)| (Formyltetrahydrofolate synthetase) (FHS) (FTHFS) Length = 555 Score = 29.6 bits (65), Expect = 3.8 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = +2 Query: 137 GFGDDNGSTEEKALKARRRESDPAAALAAAR-HEFGEHGGV 256 GFG D G+ + +KAR DPAA + A HGGV Sbjct: 300 GFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRALKMHGGV 340
>METB_MYCTU (P66875) Cystathionine gamma-synthase (EC 2.5.1.48) (CGS)| (O-succinylhomoserine (thiol)-lyase) Length = 388 Score = 29.3 bits (64), Expect = 5.0 Identities = 25/70 (35%), Positives = 33/70 (47%) Frame = +2 Query: 248 GGVNMSIEASATFTVMEPDTMRRLFTGELGPERGDLYIYSRHFNPTVLALGRQMAALEGT 427 G VN+ I AS+TF +G RG + Y+R NPT AL +AA+E Sbjct: 30 GAVNVPIYASSTFA-----------QDGVGGLRGG-FEYARTGNPTRAALEASLAAVEEG 77 Query: 428 EAAYCTASGM 457 A +SGM Sbjct: 78 AFARAFSSGM 87
>METB_MYCBO (P66876) Cystathionine gamma-synthase (EC 2.5.1.48) (CGS)| (O-succinylhomoserine (thiol)-lyase) Length = 388 Score = 29.3 bits (64), Expect = 5.0 Identities = 25/70 (35%), Positives = 33/70 (47%) Frame = +2 Query: 248 GGVNMSIEASATFTVMEPDTMRRLFTGELGPERGDLYIYSRHFNPTVLALGRQMAALEGT 427 G VN+ I AS+TF +G RG + Y+R NPT AL +AA+E Sbjct: 30 GAVNVPIYASSTFA-----------QDGVGGLRGG-FEYARTGNPTRAALEASLAAVEEG 77 Query: 428 EAAYCTASGM 457 A +SGM Sbjct: 78 AFARAFSSGM 87
>LEU3_SYMTH (Q67JY2) 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (Beta-IPM| dehydrogenase) (IMDH) (3-IPM-DH) Length = 368 Score = 29.3 bits (64), Expect = 5.0 Identities = 25/79 (31%), Positives = 38/79 (48%), Gaps = 5/79 (6%) Frame = +2 Query: 59 PTVPMAHVLAAAEPLTLLKRPTP-----QNDGFGDDNGSTEEKALKARRRESDPAAALAA 223 P V + H+LA + +L+RP ++ FGD ++E A+ P+A+L A Sbjct: 218 PDVEVEHLLADNAAMQILRRPGDFDVMLASNLFGDF--LSDEAAMLTGSIGLLPSASLGA 275 Query: 224 ARHEFGEHGGVNMSIEASA 280 AR+ FG G I SA Sbjct: 276 ARNRFGLPKGFYEPIHGSA 294
>SEMG1_HUMAN (P04279) Semenogelin-1 precursor (Semenogelin I) (SGI) [Contains:| Alpha-inhibin-92; Alpha-inhibin-31; Seminal basic protein] Length = 462 Score = 29.3 bits (64), Expect = 5.0 Identities = 19/83 (22%), Positives = 36/83 (43%) Frame = +2 Query: 110 LKRPTPQNDGFGDDNGSTEEKALKARRRESDPAAALAAARHEFGEHGGVNMSIEASATFT 289 ++ P P+ + + +N E ++ RE D + RH+ G HGG+++ I Sbjct: 388 IQAPNPKQEPWHGENAKGESG--QSTNREQDLLSHEQKGRHQHGSHGGLDIVI------- 438 Query: 290 VMEPDTMRRLFTGELGPERGDLY 358 + + D R L +R L+ Sbjct: 439 IEQEDDSDRHLAQHLNNDRNPLF 461
>RS16_RHILO (Q98E70) 30S ribosomal protein S16| Length = 133 Score = 28.9 bits (63), Expect = 6.6 Identities = 18/62 (29%), Positives = 27/62 (43%) Frame = +2 Query: 41 KHPVIRPTVPMAHVLAAAEPLTLLKRPTPQNDGFGDDNGSTEEKALKARRRESDPAAALA 220 KH + P VL + L KR N + +E+A ++ + D AAA+A Sbjct: 59 KHWLSHGAQPTDRVLRFLDEAGLAKRDARSNPKKAEPGKKAQERAALLKKAQEDAAAAVA 118 Query: 221 AA 226 AA Sbjct: 119 AA 120
>METB_HERAU (P24601) Probable cystathionine gamma-synthase (EC 2.5.1.48) (CGS)| (O-succinylhomoserine (thiol)-lyase) (Fragment) Length = 319 Score = 28.9 bits (63), Expect = 6.6 Identities = 17/34 (50%), Positives = 20/34 (58%) Frame = +2 Query: 356 YIYSRHFNPTVLALGRQMAALEGTEAAYCTASGM 457 Y YSR NPT AL +AALE + A ASG+ Sbjct: 44 YEYSRTDNPTRTALQTCLAALEEAKHALVFASGL 77
>CYS3_YEAST (P31373) Cystathionine gamma-lyase (EC 4.4.1.1)| (Gamma-cystathionase) Length = 393 Score = 28.9 bits (63), Expect = 6.6 Identities = 21/70 (30%), Positives = 30/70 (42%) Frame = +2 Query: 245 HGGVNMSIEASATFTVMEPDTMRRLFTGELGPERGDLYIYSRHFNPTVLALGRQMAALEG 424 H G ++ + S V+EP ++ F Y YSR NP L R +AALE Sbjct: 14 HAGEHVDVHGS----VIEPISLSTTFKQSSPANPIGTYEYSRSQNPNRENLERAVAALEN 69 Query: 425 TEAAYCTASG 454 + +SG Sbjct: 70 AQYGLAFSSG 79
>CGL_MOUSE (Q8VCN5) Cystathionine gamma-lyase (EC 4.4.1.1)| (Gamma-cystathionase) Length = 398 Score = 28.9 bits (63), Expect = 6.6 Identities = 15/40 (37%), Positives = 23/40 (57%) Frame = +2 Query: 338 PERGDLYIYSRHFNPTVLALGRQMAALEGTEAAYCTASGM 457 P + + YSR NPT L + +AAL+G + + ASG+ Sbjct: 51 PGQSSGFEYSRSGNPTRNCLEKAVAALDGAKHSLAFASGL 90
>ACON_MYCAV (O08451) Aconitate hydratase (EC 4.2.1.3) (Citrate hydro-lyase)| (Aconitase) Length = 961 Score = 28.9 bits (63), Expect = 6.6 Identities = 27/98 (27%), Positives = 44/98 (44%), Gaps = 1/98 (1%) Frame = +2 Query: 119 PTPQNDGFGDDNGSTEEKALKARRRESDPAAALAAARHEFG-EHGGVNMSIEASATFTVM 295 P ++G DD + + A A R S+P + EF +HG V ++ S T T Sbjct: 445 PGAPSNGHADDLPAVQSAAAHANGRPSNPVTVRSDELGEFVLDHGAVVIAAVTSCTNT-S 503 Query: 296 EPDTMRRLFTGELGPERGDLYIYSRHFNPTVLALGRQM 409 P+ M L L +R + + S+ + T +A G Q+ Sbjct: 504 NPEVM--LGAALLARQRVEKGLASKPWVKTTMAPGSQV 539
>CGL_RAT (P18757) Cystathionine gamma-lyase (EC 4.4.1.1)| (Gamma-cystathionase) (Probasin-related antigen) (PRB-RA) Length = 398 Score = 28.5 bits (62), Expect = 8.6 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +2 Query: 338 PERGDLYIYSRHFNPTVLALGRQMAALEGTEAAYCTASGM 457 P + ++YSR NPT L + +AAL+G + A G+ Sbjct: 51 PGQSSGFVYSRSGNPTRNCLEKAVAALDGAKHCLTFARGL 90
>RS16_RHIME (Q92L43) 30S ribosomal protein S16| Length = 124 Score = 28.5 bits (62), Expect = 8.6 Identities = 16/65 (24%), Positives = 29/65 (44%) Frame = +2 Query: 41 KHPVIRPTVPMAHVLAAAEPLTLLKRPTPQNDGFGDDNGSTEEKALKARRRESDPAAALA 220 +H + + P V+ + L KRP N +E+A +A+++ + AAA + Sbjct: 60 QHWIAQGAQPTDRVMRFLDQAGLAKRPARNNPTKAQPGKKAQERAAEAKQKAEEAAAAAS 119 Query: 221 AARHE 235 A E Sbjct: 120 EAAAE 124
>FTHS_STAES (Q8CNV9) Formate--tetrahydrofolate ligase (EC 6.3.4.3)| (Formyltetrahydrofolate synthetase) (FHS) (FTHFS) Length = 555 Score = 28.5 bits (62), Expect = 8.6 Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = +2 Query: 137 GFGDDNGSTEEKALKARRRESDPAAALAAAR-HEFGEHGGV 256 GFG D G+ + +KAR DP+A + A HGGV Sbjct: 300 GFGSDLGAEKFMNIKAREAGFDPSAVVVVATIRALKMHGGV 340
>FTHS_STAEQ (Q5HNH4) Formate--tetrahydrofolate ligase (EC 6.3.4.3)| (Formyltetrahydrofolate synthetase) (FHS) (FTHFS) Length = 555 Score = 28.5 bits (62), Expect = 8.6 Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = +2 Query: 137 GFGDDNGSTEEKALKARRRESDPAAALAAAR-HEFGEHGGV 256 GFG D G+ + +KAR DP+A + A HGGV Sbjct: 300 GFGSDLGAEKFMNIKAREAGFDPSAVVVVATIRALKMHGGV 340 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.315 0.137 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 39,811,888 Number of Sequences: 219361 Number of extensions: 673639 Number of successful extensions: 1894 Number of sequences better than 10.0: 29 Number of HSP's better than 10.0 without gapping: 1852 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1889 length of database: 80,573,946 effective HSP length: 102 effective length of database: 58,199,124 effective search space used: 2909956200 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits)
