ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | bast57a06 |
|---|---|
| Clone Library Name | barley_pub |
>SMT2_ORYSA (O82427) 24-methylenesterol C-methyltransferase 2 (EC 2.1.1.143)| (24-sterol C-methyltransferase 2) (Sterol-C-methyltransferase 2) Length = 363 Score = 258 bits (658), Expect = 7e-69 Identities = 121/134 (90%), Positives = 127/134 (94%) Frame = +1 Query: 70 MEPATMAWTAGLVGAGLVYWFVWVMGAAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFR 249 ME ATMAWTA VG LVYWFVWVMGAAEVKGKRAVDLKMGSIT DKV+DKYTQYWSFFR Sbjct: 1 MEAATMAWTAAGVGMALVYWFVWVMGAAEVKGKRAVDLKMGSITNDKVKDKYTQYWSFFR 60 Query: 250 RPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERV 429 RPKETATT+ASA+KVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHR+ATRVHEERV Sbjct: 61 RPKETATTEASAEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHREATRVHEERV 120 Query: 430 ADLLAARPGHRVLD 471 ADLL A+PGHR+LD Sbjct: 121 ADLLQAKPGHRLLD 134
>SMT2_ARATH (Q39227) 24-methylenesterol C-methyltransferase 2 (EC 2.1.1.143)| (24-sterol C-methyltransferase 2) (Sterol-C-methyltransferase 2) Length = 361 Score = 187 bits (476), Expect = 9e-48 Identities = 88/134 (65%), Positives = 105/134 (78%) Frame = +1 Query: 70 MEPATMAWTAGLVGAGLVYWFVWVMGAAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFR 249 M+ T+ +T LV G +YWF+ V+G AE KGKRAVDL GSI+ +KVQD Y QYWSFFR Sbjct: 1 MDSLTLFFTGALVAVG-IYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFR 59 Query: 250 RPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERV 429 RPKE T A+KVP FVDTFYNLVTDIYEWGWGQSFHFSPS+PG+SH+DATR+HEE Sbjct: 60 RPKEIET----AEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMA 115 Query: 430 ADLLAARPGHRVLD 471 DL+ +PG ++LD Sbjct: 116 VDLIQVKPGQKILD 129
>SMT3B_ARATH (Q94JS4) 24-methylenesterol C-methyltransferase 3 (EC 2.1.1.143)| (24-sterol C-methyltransferase 3) (Sterol-C-methyltransferase 3) Length = 359 Score = 181 bits (458), Expect = 1e-45 Identities = 85/134 (63%), Positives = 103/134 (76%) Frame = +1 Query: 70 MEPATMAWTAGLVGAGLVYWFVWVMGAAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFR 249 M+ + TAGL+ AG VYWF+ V+G AE KGKRA DL GSI+ +KV+D Y QYWSFFR Sbjct: 1 MDSVALYCTAGLI-AGAVYWFICVLGPAERKGKRASDLSGGSISAEKVKDNYNQYWSFFR 59 Query: 250 RPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERV 429 +PKE SA+KVP FVDTFYNLVTDIYEWGWGQSFHFSP +PG+S +DATR+HEE Sbjct: 60 KPKEIE----SAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMA 115 Query: 430 ADLLAARPGHRVLD 471 DL+ +PG ++LD Sbjct: 116 VDLIKVKPGQKILD 129
>ERG6_SCHPO (O14321) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 378 Score = 65.9 bits (159), Expect = 5e-11 Identities = 32/92 (34%), Positives = 52/92 (56%), Gaps = 3/92 (3%) Frame = +1 Query: 205 DKVQDKYTQYWSFFRRPKETATTQASADKVPAF---VDTFYNLVTDIYEWGWGQSFHFSP 375 D+ K +Y+ F+ R E + + A ++ + V+++Y+L TD+YE+GW QSFHFS Sbjct: 39 DEQSRKLQEYFEFWDRNHENESEEDRARRIDGYKSVVNSYYDLATDLYEYGWSQSFHFSR 98 Query: 376 SLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 G + + HE +A + +PG RVLD Sbjct: 99 FYKGEAFAQSIARHEHYLAYRMGIKPGSRVLD 130
>SMT1_ORYSA (Q6ZIX2) Cycloartenol-C-24-methyltransferase 1 (EC 2.1.1.41)| (24-sterol C-methyltransferase 1) (Sterol C-methyltransferase 1) Length = 344 Score = 65.9 bits (159), Expect = 5e-11 Identities = 35/97 (36%), Positives = 55/97 (56%), Gaps = 3/97 (3%) Frame = +1 Query: 190 GSITRDKVQ---DKYTQYWSFFRRPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQS 360 G IT+D+V+ D+Y +Y ++ +E A V+ +Y+L T YE+GWG+S Sbjct: 15 GKITKDEVKSAVDEYEKYHGYYGGKEE-----ARKSNYTDMVNKYYDLATSFYEYGWGES 69 Query: 361 FHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 FHF+ G S R++ + HE +A L +PG +VLD Sbjct: 70 FHFAHRWNGESLRESIKRHEHFLALQLGVKPGMKVLD 106
>ERG6_YARLI (Q6C2D9) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 381 Score = 64.3 bits (155), Expect = 1e-10 Identities = 31/71 (43%), Positives = 39/71 (54%) Frame = +1 Query: 259 ETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADL 438 E A Q + +YNLVTD YE+GWG SFHFS G + R AT HE +A Sbjct: 64 EDADEQGRIEDYAGLTKHYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHEHYLAYK 123 Query: 439 LAARPGHRVLD 471 + +PG +VLD Sbjct: 124 MGIQPGMKVLD 134
>ERG6_MAGGR (Q5EN22) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 390 Score = 61.6 bits (148), Expect = 1e-09 Identities = 31/88 (35%), Positives = 48/88 (54%), Gaps = 3/88 (3%) Frame = +1 Query: 217 DKYTQYWSFFRRPKETATTQASADKVPAFVDT---FYNLVTDIYEWGWGQSFHFSPSLPG 387 D+Y ++W + +T TT+ ++ + +YNLVTD YE+GWGQSFHF PG Sbjct: 55 DQYFRHWD--GKTAKTETTKVREERKADYATLTRQYYNLVTDFYEYGWGQSFHFCTFAPG 112 Query: 388 RSHRDATRVHEERVADLLAARPGHRVLD 471 + A +E +A + + G +VLD Sbjct: 113 ETFASAITRYEHTLAHRIGIKKGMKVLD 140
>SMT1_ARATH (Q9LM02) Cycloartenol-C-24-methyltransferase (EC 2.1.1.41)| (24-sterol C-methyltransferase 1) (Sterol C-methyltransferase 1) (STEROL METHYLTRANSFERASE 1) (CEPHALOPOD protein) Length = 336 Score = 59.3 bits (142), Expect = 5e-09 Identities = 31/85 (36%), Positives = 46/85 (54%) Frame = +1 Query: 217 DKYTQYWSFFRRPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSH 396 +KY QY F +E + V+ +Y+L T YE+GWG+SFHF+ G S Sbjct: 21 EKYEQYHVFHGGNEEERKANYTD-----MVNKYYDLATSFYEYGWGESFHFAQRWKGESL 75 Query: 397 RDATRVHEERVADLLAARPGHRVLD 471 R++ + HE +A L +PG +VLD Sbjct: 76 RESIKRHEHFLALQLGIQPGQKVLD 100
>ERG6_NEUCR (Q9P3R1) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 379 Score = 59.3 bits (142), Expect = 5e-09 Identities = 36/109 (33%), Positives = 52/109 (47%), Gaps = 1/109 (0%) Frame = +1 Query: 148 AAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFRRPKET-ATTQASADKVPAFVDTFYNL 324 +A+ G A LK + + D+Y +++ ET A +A + +YNL Sbjct: 26 SAKAAGGVAALLKKNTEAQQIAVDEYFKHFDNKTAEAETQADREARTKEYATLTRHYYNL 85 Query: 325 VTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 TDIYE+GWGQ FHF PG S A HE +A + + +VLD Sbjct: 86 ATDIYEYGWGQCFHFCRYSPGESFYQAIARHEHYLAAQIGIKKDMKVLD 134
>ERG6_CLALS (Q875K1) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 375 Score = 58.5 bits (140), Expect = 8e-09 Identities = 28/62 (45%), Positives = 34/62 (54%) Frame = +1 Query: 286 DKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRV 465 D + +YNLVTD YE+GWG SFHFS G + R AT HE +A + RV Sbjct: 67 DDYSSLTKNYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHEHFLAHKMNINENMRV 126 Query: 466 LD 471 LD Sbjct: 127 LD 128
>ERG6_DEBHA (Q6BRB7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 377 Score = 56.6 bits (135), Expect = 3e-08 Identities = 26/53 (49%), Positives = 32/53 (60%) Frame = +1 Query: 313 FYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 +YNLVTD YE+GWG SFHFS G + R AT HE +A + +VLD Sbjct: 77 YYNLVTDFYEYGWGSSFHFSRYYTGEAFRQATARHEHFLAHKMQINENMKVLD 129
>ERG6_CANAL (O74198) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 376 Score = 56.2 bits (134), Expect = 4e-08 Identities = 26/53 (49%), Positives = 32/53 (60%) Frame = +1 Query: 313 FYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 +YNLVTD YE+GWG SFHFS G + R AT HE +A + +VLD Sbjct: 76 YYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHEHFLAHKMNLNENMKVLD 128
>ERG6_CANGA (Q6FRZ7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 372 Score = 56.2 bits (134), Expect = 4e-08 Identities = 37/109 (33%), Positives = 55/109 (50%) Frame = +1 Query: 145 GAAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFRRPKETATTQASADKVPAFVDTFYNL 324 GA G A+ K + R+ V KY ++W + + A + D A ++YN+ Sbjct: 21 GAESRTGLTALLSKNRNAQREAVS-KYLRHWD--GKTDKEAEDRRLEDYNEA-THSYYNV 76 Query: 325 VTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 VTD YE+GWG SFHFS G + + A HE+ +A + + G VLD Sbjct: 77 VTDFYEYGWGSSFHFSRFYKGENFQAAMARHEQYLAYMAGIKKGDLVLD 125
>ERG6_YEAST (P25087) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 382 Score = 53.1 bits (126), Expect = 3e-07 Identities = 37/103 (35%), Positives = 50/103 (48%) Frame = +1 Query: 163 GKRAVDLKMGSITRDKVQDKYTQYWSFFRRPKETATTQASADKVPAFVDTFYNLVTDIYE 342 G A+ K S ++ VQ KY + W R + A + D A ++YN+VTD YE Sbjct: 26 GLSALMSKNNSAQKEAVQ-KYLRNWD--GRTDKDAEERRLEDYNEA-THSYYNVVTDFYE 81 Query: 343 WGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 +GWG SFHFS G S + HE +A + G VLD Sbjct: 82 YGWGSSFHFSRFYKGESFAASIARHEHYLAYKAGIQRGDLVLD 124
>ERG6_ASHGO (Q759S7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 373 Score = 50.1 bits (118), Expect = 3e-06 Identities = 24/54 (44%), Positives = 30/54 (55%) Frame = +1 Query: 310 TFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 ++YN+VTD YE+GWG SFHFS G S + HE +A G VLD Sbjct: 73 SYYNVVTDFYEYGWGASFHFSRFYTGESFAMSMARHEHYLAHRAGITSGDLVLD 126
>ERG6_KLULA (Q6CYB3) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 371 Score = 49.3 bits (116), Expect = 5e-06 Identities = 22/54 (40%), Positives = 30/54 (55%) Frame = +1 Query: 310 TFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 ++YN+VTD YE+GWG SFHFS G S + HE +A + +LD Sbjct: 72 SYYNVVTDFYEYGWGSSFHFSRFFKGESFSASVARHEHYLAYKAGIKENDLILD 125
>ERG6_PNECA (Q96WX4) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 377 Score = 48.5 bits (114), Expect = 8e-06 Identities = 24/53 (45%), Positives = 27/53 (50%) Frame = +1 Query: 313 FYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVADLLAARPGHRVLD 471 +YNLVTD YE+GW SFHF S A HE +A R G VLD Sbjct: 80 YYNLVTDFYEYGWSTSFHFCRFAKDESFSQAIARHEHYIALHAGIREGETVLD 132
>AKA14_RAT (O35817) A-kinase anchor protein 14 (Testis-specific| A-kinase-anchoring protein TAKAP-80) (TAKAP-1.2) Length = 502 Score = 30.0 bits (66), Expect = 3.1 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = -3 Query: 164 PFTSAAPITHTNQ*TSPAPTSPAVHAIVAGSILPSFP 54 P AAP+ HT Q + AP +P++ + ++P P Sbjct: 181 PVAHAAPVAHTAQVSHAAPVAPSLPVVPPAPVVPLAP 217
>RAD5_DEBHA (Q6BIP2) DNA repair protein RAD5 (EC 3.6.1.-)| Length = 1153 Score = 30.0 bits (66), Expect = 3.1 Identities = 18/45 (40%), Positives = 22/45 (48%) Frame = +1 Query: 268 TTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRD 402 TTQ A+ + D F N + DI+EW S H S SLP D Sbjct: 470 TTQDKAN-IQERNDAFMNPLWDIFEWPKDTSMHKSESLPTEDRMD 513
>CHVA_AGRTU (P0A2V1) Beta-(1-->2)glucan export ATP-binding protein chvA| (Attachment protein) Length = 588 Score = 29.3 bits (64), Expect = 5.3 Identities = 11/17 (64%), Positives = 14/17 (82%), Gaps = 2/17 (11%) Frame = +1 Query: 103 LVGAGLVYWFV--WVMG 147 L+G G+VYWF+ WVMG Sbjct: 162 LIGLGIVYWFIGKWVMG 178
>CHVA_AGRT5 (P0A2V0) Beta-(1-->2)glucan export ATP-binding protein chvA| (Attachment protein) Length = 588 Score = 29.3 bits (64), Expect = 5.3 Identities = 11/17 (64%), Positives = 14/17 (82%), Gaps = 2/17 (11%) Frame = +1 Query: 103 LVGAGLVYWFV--WVMG 147 L+G G+VYWF+ WVMG Sbjct: 162 LIGLGIVYWFIGKWVMG 178
>DING_BACSU (P54394) Probable ATP-dependent helicase dinG homolog (EC 3.6.1.-)| Length = 931 Score = 28.9 bits (63), Expect = 6.9 Identities = 21/76 (27%), Positives = 29/76 (38%) Frame = +1 Query: 223 YTQYWSFFRRPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRD 402 YT++ SF R E + + D+ N +I W G S +PG RD Sbjct: 211 YTRFSSFSVREPEAIDVRINEDE---------NFSFEIESWEAGNEKALSELMPGYEKRD 261 Query: 403 ATRVHEERVADLLAAR 450 + VAD A R Sbjct: 262 GQMMMMREVADAFANR 277
>YDJZ_ECOLI (P76221) UPF0043 inner membrane protein ydjZ| Length = 235 Score = 28.9 bits (63), Expect = 6.9 Identities = 15/43 (34%), Positives = 23/43 (53%), Gaps = 3/43 (6%) Frame = +1 Query: 85 MAWTAGLVGAGLVYWFVWVMGAAEVK---GKRAVDLKMGSITR 204 ++WT+ + GA L ++ VMG V+ GK +D G TR Sbjct: 101 LSWTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTR 143
>APU_THESA (P36905) Amylopullulanase precursor (Alpha-amylase/pullulanase)| [Includes: Alpha-amylase (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (EC 3.2.1.41) (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosida Length = 1279 Score = 28.9 bits (63), Expect = 6.9 Identities = 16/60 (26%), Positives = 27/60 (45%), Gaps = 3/60 (5%) Frame = +1 Query: 190 GSITRDKVQDKYTQYWSFFRRPKETATTQ---ASADKVPAFVDTFYNLVTDIYEWGWGQS 360 G++T + D Y +W + T +T+ S DK+P V T + + +W G S Sbjct: 107 GNLTLNLTNDSYVTFWFDYNTQSVTDSTKYTPISNDKLPRLVGTIQSAIGAGKDWDPGTS 166
>LDB3_HUMAN (O75112) LIM domain-binding protein 3 (Z-band alternatively spliced| PDZ-motif protein) (Protein cypher) Length = 727 Score = 28.5 bits (62), Expect = 9.1 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = -3 Query: 155 SAAPITHTNQ*TSPAPTSPAVHAIVAGSILPS 60 S+AP THT+ PA +P + SI PS Sbjct: 375 SSAPATHTSYSEGPAAPAPKPRVVTTASIRPS 406 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 30,928,491 Number of Sequences: 219361 Number of extensions: 548823 Number of successful extensions: 2163 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 2137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 2160 length of database: 80,573,946 effective HSP length: 103 effective length of database: 57,979,763 effective search space used: 3072927439 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
