ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | bast55f04 |
|---|---|
| Clone Library Name | barley_pub |
>SMT2_ORYSA (O82427) 24-methylenesterol C-methyltransferase 2 (EC 2.1.1.143)| (24-sterol C-methyltransferase 2) (Sterol-C-methyltransferase 2) Length = 363 Score = 241 bits (616), Expect = 5e-64 Identities = 114/124 (91%), Positives = 118/124 (95%) Frame = +1 Query: 73 MEPATMAWTAGLVGAGLVYWFVWVMGAAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFR 252 ME ATMAWTA VG LVYWFVWVMGAAEVKGKRAVDLKMGSIT DKV+DKYTQYWSFFR Sbjct: 1 MEAATMAWTAAGVGMALVYWFVWVMGAAEVKGKRAVDLKMGSITNDKVKDKYTQYWSFFR 60 Query: 253 RPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERV 432 RPKETATT+ASA+KVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHR+ATRVHEERV Sbjct: 61 RPKETATTEASAEKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHREATRVHEERV 120 Query: 433 ADLL 444 ADLL Sbjct: 121 ADLL 124
>SMT2_ARATH (Q39227) 24-methylenesterol C-methyltransferase 2 (EC 2.1.1.143)| (24-sterol C-methyltransferase 2) (Sterol-C-methyltransferase 2) Length = 361 Score = 176 bits (447), Expect = 2e-44 Identities = 84/124 (67%), Positives = 98/124 (79%) Frame = +1 Query: 73 MEPATMAWTAGLVGAGLVYWFVWVMGAAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFR 252 M+ T+ +T LV G +YWF+ V+G AE KGKRAVDL GSI+ +KVQD Y QYWSFFR Sbjct: 1 MDSLTLFFTGALVAVG-IYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFR 59 Query: 253 RPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERV 432 RPKE T A+KVP FVDTFYNLVTDIYEWGWGQSFHFSPS+PG+SH+DATR+HEE Sbjct: 60 RPKEIET----AEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMA 115 Query: 433 ADLL 444 DL+ Sbjct: 116 VDLI 119
>SMT3B_ARATH (Q94JS4) 24-methylenesterol C-methyltransferase 3 (EC 2.1.1.143)| (24-sterol C-methyltransferase 3) (Sterol-C-methyltransferase 3) Length = 359 Score = 169 bits (429), Expect = 2e-42 Identities = 81/124 (65%), Positives = 96/124 (77%) Frame = +1 Query: 73 MEPATMAWTAGLVGAGLVYWFVWVMGAAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFR 252 M+ + TAGL+ AG VYWF+ V+G AE KGKRA DL GSI+ +KV+D Y QYWSFFR Sbjct: 1 MDSVALYCTAGLI-AGAVYWFICVLGPAERKGKRASDLSGGSISAEKVKDNYNQYWSFFR 59 Query: 253 RPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERV 432 +PKE SA+KVP FVDTFYNLVTDIYEWGWGQSFHFSP +PG+S +DATR+HEE Sbjct: 60 KPKEIE----SAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMA 115 Query: 433 ADLL 444 DL+ Sbjct: 116 VDLI 119
>SMT1_ORYSA (Q6ZIX2) Cycloartenol-C-24-methyltransferase 1 (EC 2.1.1.41)| (24-sterol C-methyltransferase 1) (Sterol C-methyltransferase 1) Length = 344 Score = 55.5 bits (132), Expect = 6e-08 Identities = 29/84 (34%), Positives = 47/84 (55%), Gaps = 3/84 (3%) Frame = +1 Query: 193 GSITRDKVQ---DKYTQYWSFFRRPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQS 363 G IT+D+V+ D+Y +Y ++ +E A V+ +Y+L T YE+GWG+S Sbjct: 15 GKITKDEVKSAVDEYEKYHGYYGGKEE-----ARKSNYTDMVNKYYDLATSFYEYGWGES 69 Query: 364 FHFSPSLPGRSHRDATRVHEERVA 435 FHF+ G S R++ + HE +A Sbjct: 70 FHFAHRWNGESLRESIKRHEHFLA 93
>ERG6_NEUCR (Q9P3R1) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 379 Score = 55.1 bits (131), Expect = 8e-08 Identities = 33/96 (34%), Positives = 46/96 (47%), Gaps = 1/96 (1%) Frame = +1 Query: 151 AAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFRRPKET-ATTQASADKVPAFVDTFYNL 327 +A+ G A LK + + D+Y +++ ET A +A + +YNL Sbjct: 26 SAKAAGGVAALLKKNTEAQQIAVDEYFKHFDNKTAEAETQADREARTKEYATLTRHYYNL 85 Query: 328 VTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 TDIYE+GWGQ FHF PG S A HE +A Sbjct: 86 ATDIYEYGWGQCFHFCRYSPGESFYQAIARHEHYLA 121
>ERG6_YARLI (Q6C2D9) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 381 Score = 55.1 bits (131), Expect = 8e-08 Identities = 26/58 (44%), Positives = 31/58 (53%) Frame = +1 Query: 262 ETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 E A Q + +YNLVTD YE+GWG SFHFS G + R AT HE +A Sbjct: 64 EDADEQGRIEDYAGLTKHYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHEHYLA 121
>ERG6_SCHPO (O14321) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 378 Score = 54.7 bits (130), Expect = 1e-07 Identities = 26/79 (32%), Positives = 44/79 (55%), Gaps = 3/79 (3%) Frame = +1 Query: 208 DKVQDKYTQYWSFFRRPKETATTQASADKVPAF---VDTFYNLVTDIYEWGWGQSFHFSP 378 D+ K +Y+ F+ R E + + A ++ + V+++Y+L TD+YE+GW QSFHFS Sbjct: 39 DEQSRKLQEYFEFWDRNHENESEEDRARRIDGYKSVVNSYYDLATDLYEYGWSQSFHFSR 98 Query: 379 SLPGRSHRDATRVHEERVA 435 G + + HE +A Sbjct: 99 FYKGEAFAQSIARHEHYLA 117
>ERG6_MAGGR (Q5EN22) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 390 Score = 54.3 bits (129), Expect = 1e-07 Identities = 27/75 (36%), Positives = 41/75 (54%), Gaps = 3/75 (4%) Frame = +1 Query: 220 DKYTQYWSFFRRPKETATTQASADKVPAFVDT---FYNLVTDIYEWGWGQSFHFSPSLPG 390 D+Y ++W + +T TT+ ++ + +YNLVTD YE+GWGQSFHF PG Sbjct: 55 DQYFRHWD--GKTAKTETTKVREERKADYATLTRQYYNLVTDFYEYGWGQSFHFCTFAPG 112 Query: 391 RSHRDATRVHEERVA 435 + A +E +A Sbjct: 113 ETFASAITRYEHTLA 127
>ERG6_CLALS (Q875K1) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 375 Score = 53.9 bits (128), Expect = 2e-07 Identities = 24/49 (48%), Positives = 29/49 (59%) Frame = +1 Query: 289 DKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 D + +YNLVTD YE+GWG SFHFS G + R AT HE +A Sbjct: 67 DDYSSLTKNYYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHEHFLA 115
>ERG6_CANAL (O74198) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 376 Score = 52.8 bits (125), Expect = 4e-07 Identities = 23/40 (57%), Positives = 27/40 (67%) Frame = +1 Query: 316 FYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 +YNLVTD YE+GWG SFHFS G + R AT HE +A Sbjct: 76 YYNLVTDFYEYGWGSSFHFSRYYKGEAFRQATARHEHFLA 115
>ERG6_DEBHA (Q6BRB7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 377 Score = 52.8 bits (125), Expect = 4e-07 Identities = 23/40 (57%), Positives = 27/40 (67%) Frame = +1 Query: 316 FYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 +YNLVTD YE+GWG SFHFS G + R AT HE +A Sbjct: 77 YYNLVTDFYEYGWGSSFHFSRYYTGEAFRQATARHEHFLA 116
>ERG6_CANGA (Q6FRZ7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 372 Score = 50.4 bits (119), Expect = 2e-06 Identities = 33/96 (34%), Positives = 49/96 (51%) Frame = +1 Query: 148 GAAEVKGKRAVDLKMGSITRDKVQDKYTQYWSFFRRPKETATTQASADKVPAFVDTFYNL 327 GA G A+ K + R+ V KY ++W + + A + D A ++YN+ Sbjct: 21 GAESRTGLTALLSKNRNAQREAVS-KYLRHWD--GKTDKEAEDRRLEDYNEA-THSYYNV 76 Query: 328 VTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 VTD YE+GWG SFHFS G + + A HE+ +A Sbjct: 77 VTDFYEYGWGSSFHFSRFYKGENFQAAMARHEQYLA 112
>ERG6_YEAST (P25087) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 382 Score = 49.7 bits (117), Expect = 3e-06 Identities = 33/90 (36%), Positives = 45/90 (50%) Frame = +1 Query: 166 GKRAVDLKMGSITRDKVQDKYTQYWSFFRRPKETATTQASADKVPAFVDTFYNLVTDIYE 345 G A+ K S ++ VQ KY + W R + A + D A ++YN+VTD YE Sbjct: 26 GLSALMSKNNSAQKEAVQ-KYLRNWD--GRTDKDAEERRLEDYNEA-THSYYNVVTDFYE 81 Query: 346 WGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 +GWG SFHFS G S + HE +A Sbjct: 82 YGWGSSFHFSRFYKGESFAASIARHEHYLA 111
>SMT1_ARATH (Q9LM02) Cycloartenol-C-24-methyltransferase (EC 2.1.1.41)| (24-sterol C-methyltransferase 1) (Sterol C-methyltransferase 1) (STEROL METHYLTRANSFERASE 1) (CEPHALOPOD protein) Length = 336 Score = 48.9 bits (115), Expect = 6e-06 Identities = 25/72 (34%), Positives = 38/72 (52%) Frame = +1 Query: 220 DKYTQYWSFFRRPKETATTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSH 399 +KY QY F +E + V+ +Y+L T YE+GWG+SFHF+ G S Sbjct: 21 EKYEQYHVFHGGNEEERKANYTD-----MVNKYYDLATSFYEYGWGESFHFAQRWKGESL 75 Query: 400 RDATRVHEERVA 435 R++ + HE +A Sbjct: 76 RESIKRHEHFLA 87
>ERG6_KLULA (Q6CYB3) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 371 Score = 47.8 bits (112), Expect = 1e-05 Identities = 20/41 (48%), Positives = 26/41 (63%) Frame = +1 Query: 313 TFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 ++YN+VTD YE+GWG SFHFS G S + HE +A Sbjct: 72 SYYNVVTDFYEYGWGSSFHFSRFFKGESFSASVARHEHYLA 112
>ERG6_ASHGO (Q759S7) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 373 Score = 46.2 bits (108), Expect = 4e-05 Identities = 20/41 (48%), Positives = 26/41 (63%) Frame = +1 Query: 313 TFYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 ++YN+VTD YE+GWG SFHFS G S + HE +A Sbjct: 73 SYYNVVTDFYEYGWGASFHFSRFYTGESFAMSMARHEHYLA 113
>ERG6_PNECA (Q96WX4) Sterol 24-C-methyltransferase (EC 2.1.1.41)| (Delta(24)-sterol C-methyltransferase) Length = 377 Score = 43.1 bits (100), Expect = 3e-04 Identities = 19/40 (47%), Positives = 22/40 (55%) Frame = +1 Query: 316 FYNLVTDIYEWGWGQSFHFSPSLPGRSHRDATRVHEERVA 435 +YNLVTD YE+GW SFHF S A HE +A Sbjct: 80 YYNLVTDFYEYGWSTSFHFCRFAKDESFSQAIARHEHYIA 119
>RAD5_DEBHA (Q6BIP2) DNA repair protein RAD5 (EC 3.6.1.-)| Length = 1153 Score = 30.0 bits (66), Expect = 2.8 Identities = 18/45 (40%), Positives = 22/45 (48%) Frame = +1 Query: 271 TTQASADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSLPGRSHRD 405 TTQ A+ + D F N + DI+EW S H S SLP D Sbjct: 470 TTQDKAN-IQERNDAFMNPLWDIFEWPKDTSMHKSESLPTEDRMD 513
>AKA14_RAT (O35817) A-kinase anchor protein 14 (Testis-specific| A-kinase-anchoring protein TAKAP-80) (TAKAP-1.2) Length = 502 Score = 30.0 bits (66), Expect = 2.8 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = -1 Query: 167 PFTSAAPITHTNQ*TSPAPTSPAVHAIVAGSILPSFP 57 P AAP+ HT Q + AP +P++ + ++P P Sbjct: 181 PVAHAAPVAHTAQVSHAAPVAPSLPVVPPAPVVPLAP 217
>CHVA_AGRTU (P0A2V1) Beta-(1-->2)glucan export ATP-binding protein chvA| (Attachment protein) Length = 588 Score = 29.3 bits (64), Expect = 4.8 Identities = 11/17 (64%), Positives = 14/17 (82%), Gaps = 2/17 (11%) Frame = +1 Query: 106 LVGAGLVYWFV--WVMG 150 L+G G+VYWF+ WVMG Sbjct: 162 LIGLGIVYWFIGKWVMG 178
>CHVA_AGRT5 (P0A2V0) Beta-(1-->2)glucan export ATP-binding protein chvA| (Attachment protein) Length = 588 Score = 29.3 bits (64), Expect = 4.8 Identities = 11/17 (64%), Positives = 14/17 (82%), Gaps = 2/17 (11%) Frame = +1 Query: 106 LVGAGLVYWFV--WVMG 150 L+G G+VYWF+ WVMG Sbjct: 162 LIGLGIVYWFIGKWVMG 178
>YDJZ_ECOLI (P76221) UPF0043 inner membrane protein ydjZ| Length = 235 Score = 28.9 bits (63), Expect = 6.3 Identities = 15/43 (34%), Positives = 23/43 (53%), Gaps = 3/43 (6%) Frame = +1 Query: 88 MAWTAGLVGAGLVYWFVWVMGAAEVK---GKRAVDLKMGSITR 207 ++WT+ + GA L ++ VMG V+ GK +D G TR Sbjct: 101 LSWTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTR 143
>APU_THESA (P36905) Amylopullulanase precursor (Alpha-amylase/pullulanase)| [Includes: Alpha-amylase (EC 3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (EC 3.2.1.41) (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosida Length = 1279 Score = 28.9 bits (63), Expect = 6.3 Identities = 16/60 (26%), Positives = 27/60 (45%), Gaps = 3/60 (5%) Frame = +1 Query: 193 GSITRDKVQDKYTQYWSFFRRPKETATTQ---ASADKVPAFVDTFYNLVTDIYEWGWGQS 363 G++T + D Y +W + T +T+ S DK+P V T + + +W G S Sbjct: 107 GNLTLNLTNDSYVTFWFDYNTQSVTDSTKYTPISNDKLPRLVGTIQSAIGAGKDWDPGTS 166
>LDB3_HUMAN (O75112) LIM domain-binding protein 3 (Z-band alternatively spliced| PDZ-motif protein) (Protein cypher) Length = 727 Score = 28.5 bits (62), Expect = 8.2 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = -1 Query: 158 SAAPITHTNQ*TSPAPTSPAVHAIVAGSILPS 63 S+AP THT+ PA +P + SI PS Sbjct: 375 SSAPATHTSYSEGPAAPAPKPRVVTTASIRPS 406 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 27,833,934 Number of Sequences: 219361 Number of extensions: 499872 Number of successful extensions: 2022 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 1996 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 2019 length of database: 80,573,946 effective HSP length: 102 effective length of database: 58,199,124 effective search space used: 2793557952 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
