ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bast50b05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RAD9A_HUMAN (Q99638) Cell cycle checkpoint control protein RAD9A... 30 2.3
2ROM1_YEAST (P53046) RHO1 GDP-GTP exchange protein 1 (Protein kin... 30 3.1
3GAG_IPMA (P11365) Retrovirus-related Gag polyprotein [Contains: ... 30 4.0
4VRP1_YEAST (P37370) Verprolin 30 4.0
5COG1_MOUSE (Q9Z160) Conserved oligomeric Golgi complex component... 29 6.8

>RAD9A_HUMAN (Q99638) Cell cycle checkpoint control protein RAD9A (EC 3.1.11.2)|
           (DNA repair exonuclease rad9 homolog A) (hRAD9)
          Length = 391

 Score = 30.4 bits (67), Expect = 2.3
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
 Frame = -1

Query: 445 PDRPDKTGPWSQKE-----GTCPASDPRNK*NNVKSSGISLAPVRAPTYPTPL 302
           P  P   GP S++E      T P + P  K  ++    I LAPVR+P  P+P+
Sbjct: 331 PQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSI-LAPVRSPQGPSPV 382



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>ROM1_YEAST (P53046) RHO1 GDP-GTP exchange protein 1 (Protein kinase C|
           suppressor SKC1)
          Length = 1155

 Score = 30.0 bits (66), Expect = 3.1
 Identities = 29/115 (25%), Positives = 46/115 (40%), Gaps = 13/115 (11%)
 Frame = -1

Query: 358 KSSGISLA-PVRAPTYPTPLKSFHKVGLESS--------STGSSFPADSAKPVPLAVVSL 206
           K+SG  +  P +  T PTP  + H + L SS        ST S F  +S K       + 
Sbjct: 163 KNSGSHMGDPNQLSTPPTPKSAGHTMELHSSFNGKHSSSSTSSLFALESLKTQNRRSSNS 222

Query: 205 DSRQGQWESR*SIHARH*LDDE----AFGYLKRVIVTPAVYPRLVEFLHFDIQST 53
            +   Q+    + H RH    +    +   +  +  TP VYP L+  +    + T
Sbjct: 223 SNHSSQYRRHTNQHQRHHSRSKSSPVSLTEISMIKGTPLVYPALLSLIAIKFKQT 277



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>GAG_IPMA (P11365) Retrovirus-related Gag polyprotein [Contains: Protease (EC|
           3.4.23.-)]
          Length = 827

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 23/72 (31%), Positives = 35/72 (48%)
 Frame = -1

Query: 436 PDKTGPWSQKEGTCPASDPRNK*NNVKSSGISLAPVRAPTYPTPLKSFHKVGLESSSTGS 257
           PD    +  K G+   S PR++   +K  G  L+  R  +   P K+ HKV L ++S   
Sbjct: 513 PDSQSAYVPKNGS---SGPRSQ--GLKDMGTGLSGPRKQSERRPRKT-HKVDLRAASDFL 566

Query: 256 SFPADSAKPVPL 221
             P  S +PVP+
Sbjct: 567 LMPQMSIQPVPV 578



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>VRP1_YEAST (P37370) Verprolin|
          Length = 817

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 21/66 (31%), Positives = 29/66 (43%)
 Frame = -1

Query: 394 PASDPRNK*NNVKSSGISLAPVRAPTYPTPLKSFHKVGLESSSTGSSFPADSAKPVPLAV 215
           P+  P ++ +  KSS ISL  V AP  P+           S  T  S P  +  P P   
Sbjct: 197 PSVRPAHRSHQRKSSNISLPSVSAPPLPS----------ASLPTHVSNPPQAPPPPPTPT 246

Query: 214 VSLDSR 197
           + LDS+
Sbjct: 247 IGLDSK 252



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>COG1_MOUSE (Q9Z160) Conserved oligomeric Golgi complex component 1 (Low|
           density lipoprotein receptor defect B-complementing
           protein)
          Length = 980

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +2

Query: 191 SLSTIQRNHSQGNGLGGISGERRPC 265
           +L T+ R H  G G+G + GE + C
Sbjct: 293 TLETVTRQHPTGKGIGALQGEMKLC 317


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,164,766
Number of Sequences: 219361
Number of extensions: 1459005
Number of successful extensions: 3502
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 3408
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3501
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3014947676
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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