ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bast40d01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SC5D_TOBAC (Q9ZT29) Delta-7-sterol-C5(6)-desaturase (EC 1.3.3.-)... 156 3e-38
2SC5D1_ARATH (Q39208) Delta-7-sterol-C5(6)-desaturase 1 (EC 1.3.3... 140 1e-33
3SC5D2_ARATH (Q9M883) Putative delta-7-sterol-C5(6)-desaturase 2 ... 132 5e-31
4SGLT_VIBPA (P96169) Sodium/glucose cotransporter (Na(+)/glucose ... 33 0.43
5BSU1_SCHPO (P33532) Vitamin B6 transporter bsu1 (Vitamin B6 upta... 31 1.6
6NUOH_MYCTU (P65561) NADH-quinone oxidoreductase chain H (EC 1.6.... 30 3.6
7NUOH_MYCBO (P65562) NADH-quinone oxidoreductase chain H (EC 1.6.... 30 3.6
8WBP11_BRARE (Q6P0D5) WW domain-binding protein 11 (WBP-11) 30 3.6
9PRP28_CRYNE (Q5KNF8) Pre-mRNA splicing ATP-dependent RNA helicas... 29 4.7
10ATG13_EMENI (Q5BBK4) Autophagy-related protein 13 29 6.2
11RPGF3_MOUSE (Q8VCC8) Rap guanine nucleotide exchange factor 3 (c... 28 8.1
12RPGF3_RAT (Q9Z1C8) Rap guanine nucleotide exchange factor 3 (cAM... 28 8.1

>SC5D_TOBAC (Q9ZT29) Delta-7-sterol-C5(6)-desaturase (EC 1.3.3.-) (Delta-7-C-5|
           sterol desaturase) (Delta7-sterol-C5-desaturase)
          Length = 271

 Score =  156 bits (394), Expect = 3e-38
 Identities = 72/115 (62%), Positives = 86/115 (74%)
 Frame = +1

Query: 103 EDYWGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCF 282
           EDY   F EET +YN + L   +P    WW  LPH L+ WLRN IGG LLYF +GFLWCF
Sbjct: 2   EDYLKQFVEETSFYNRLVLGTFMPES--WWGPLPHMLQGWLRNYIGGVLLYFISGFLWCF 59

Query: 283 VIYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCF 447
            IY+ KRN YIPKDA+P+ +AM  QI VA KAMP+YCALPS+SE+MIE+GWT+CF
Sbjct: 60  YIYHLKRNVYIPKDAIPSNKAMLLQISVAMKAMPWYCALPSLSEYMIENGWTKCF 114



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>SC5D1_ARATH (Q39208) Delta-7-sterol-C5(6)-desaturase 1 (EC 1.3.3.-)|
           (Delta-7-C-5 sterol desaturase 1)
           (Delta7-sterol-C5-desaturase 1) (STEROL1 protein) (Dwarf
           7 protein)
          Length = 281

 Score =  140 bits (354), Expect = 1e-33
 Identities = 64/109 (58%), Positives = 81/109 (74%)
 Frame = +1

Query: 121 FREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFVIYYWK 300
           F +ET +YN I LS ++P     W  LPH L++WLRN + G LLYF +GFLWCF IYY K
Sbjct: 11  FVDETSFYNRIVLSHLLPAN--LWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYYLK 68

Query: 301 RNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCF 447
            N Y+PKDA+PT++AM+ Q+ VA KAMP+Y  LP+VSE MIE GWT+CF
Sbjct: 69  INVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCF 117



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>SC5D2_ARATH (Q9M883) Putative delta-7-sterol-C5(6)-desaturase 2 (EC 1.3.3.-)|
           (Delta-7-C-5 sterol desaturase 2)
           (Delta7-sterol-C5-desaturase 2) (Homolog of DWF7
           protein)
          Length = 279

 Score =  132 bits (331), Expect = 5e-31
 Identities = 61/114 (53%), Positives = 80/114 (70%)
 Frame = +1

Query: 106 DYWGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV 285
           DY      ET +YN + LS ++P     W  LPH L++WLRN + G +LYF +GFLWCF 
Sbjct: 7   DYNDQIVNETSFYNRMVLSHLLPVN--LWEPLPHFLQTWLRNYLAGNILYFISGFLWCFY 64

Query: 286 IYYWKRNAYIPKDAVPTVEAMKKQIIVASKAMPFYCALPSVSEHMIESGWTRCF 447
           IYY K N Y+PK+++PT +AM  QI VA KAMP+Y  LP+VSE+MIE GWT+C+
Sbjct: 65  IYYLKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCY 118



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>SGLT_VIBPA (P96169) Sodium/glucose cotransporter (Na(+)/glucose symporter)|
          Length = 543

 Score = 32.7 bits (73), Expect = 0.43
 Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 5/60 (8%)
 Frame = +1

Query: 265 GFLWCFVIYYWKRNAYIPKD--AVPTVEAMKKQIIVAS---KAMPFYCALPSVSEHMIES 429
           G LW   +YYW  N YI +   A  +V   +K I+ A+     +PF   LP ++ ++I S
Sbjct: 254 GGLWVANLYYWGFNQYIIQRTLAAKSVSEAQKGIVFAAFLKLIVPFLVVLPGIAAYVITS 313



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>BSU1_SCHPO (P33532) Vitamin B6 transporter bsu1 (Vitamin B6 uptake protein 1)|
           (Changed amiloride resistance protein 1) (Amiloride
           transporter car1)
          Length = 526

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 14/60 (23%), Positives = 28/60 (46%)
 Frame = +1

Query: 115 GLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFVIYY 294
           G+ R E  + + +F++ + P G   +    HP   W+   +G  ++  A G  W  ++ Y
Sbjct: 379 GVARPEARFTSALFITLLFPAGMFLFAFTCHPPFPWMSPIVGNSMVTVANGHNWMCILNY 438



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>NUOH_MYCTU (P65561) NADH-quinone oxidoreductase chain H (EC 1.6.99.5) (NADH|
           dehydrogenase I, chain H) (NDH-1, chain H)
          Length = 410

 Score = 29.6 bits (65), Expect = 3.6
 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +1

Query: 184 GWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV-IYYWKRNAYIPK 321
           G W A P PL  W     G + L + T  +W F+ IY+W R A +P+
Sbjct: 265 GGWHA-PWPLNMWASANTGWWPLIWFTAKVWGFLFIYFWLR-ATLPR 309



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>NUOH_MYCBO (P65562) NADH-quinone oxidoreductase chain H (EC 1.6.99.5) (NADH|
           dehydrogenase I, chain H) (NDH-1, chain H)
          Length = 410

 Score = 29.6 bits (65), Expect = 3.6
 Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +1

Query: 184 GWWRALPHPLRSWLRNCIGGYLLYFATGFLWCFV-IYYWKRNAYIPK 321
           G W A P PL  W     G + L + T  +W F+ IY+W R A +P+
Sbjct: 265 GGWHA-PWPLNMWASANTGWWPLIWFTAKVWGFLFIYFWLR-ATLPR 309



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>WBP11_BRARE (Q6P0D5) WW domain-binding protein 11 (WBP-11)|
          Length = 640

 Score = 29.6 bits (65), Expect = 3.6
 Identities = 13/21 (61%), Positives = 14/21 (66%)
 Frame = -1

Query: 224 SQERSGCGSARHQPPPPGTTA 162
           S E +  GSA   PPPPGTTA
Sbjct: 512 SNEGAPSGSALQMPPPPGTTA 532



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>PRP28_CRYNE (Q5KNF8) Pre-mRNA splicing ATP-dependent RNA helicase PRP28 (EC|
           3.6.1.-)
          Length = 738

 Score = 29.3 bits (64), Expect = 4.7
 Identities = 16/47 (34%), Positives = 22/47 (46%)
 Frame = +1

Query: 112 WGLFREETDWYNEIFLSAVVPGGGGWWRALPHPLRSWLRNCIGGYLL 252
           W +FRE+         S    GGG     +PHPLR+W  + I   +L
Sbjct: 294 WRIFRED--------FSIAARGGG-----IPHPLRNWRESAIPSQIL 327



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>ATG13_EMENI (Q5BBK4) Autophagy-related protein 13|
          Length = 974

 Score = 28.9 bits (63), Expect = 6.2
 Identities = 13/39 (33%), Positives = 21/39 (53%)
 Frame = -1

Query: 215 RSGCGSARHQPPPPGTTALRKISLYQSVSSRNRPQ*SSP 99
           R+    +RH PPP  TT+ RK   +   ++ + P  +SP
Sbjct: 470 RTSLSDSRHMPPPSVTTSSRKPPSFGPDNANSSPNSASP 508



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>RPGF3_MOUSE (Q8VCC8) Rap guanine nucleotide exchange factor 3 (cAMP-regulated|
           guanine nucleotide exchange factor I) (cAMP-GEFI)
           (Exchange factor directly activated by cAMP 1) (Epac 1)
          Length = 876

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +3

Query: 303 QRLHPQRCCPYSRSYEEANNCCIKG 377
           +R+H  RCC Y   +E     CI+G
Sbjct: 4   KRMHRPRCCSYQLVFEHRRPSCIQG 28



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>RPGF3_RAT (Q9Z1C8) Rap guanine nucleotide exchange factor 3 (cAMP-regulated|
           guanine nucleotide exchange factor I) (cAMP-GEFI)
           (Exchange factor directly activated by cAMP 1) (Epac 1)
          Length = 884

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +3

Query: 303 QRLHPQRCCPYSRSYEEANNCCIKG 377
           +R+H  RCC Y   +E     CI+G
Sbjct: 4   KRMHRPRCCSYQLVFEHRRPSCIQG 28


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,594,642
Number of Sequences: 219361
Number of extensions: 721589
Number of successful extensions: 2480
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2409
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2476
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2735358828
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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